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Protein

Glutathione peroxidase-like peroxiredoxin HYR1

Gene

HYR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYR1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYR1/GPX3 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598'), which causes its nuclear accumulation and activation, and a reduced Cys-36 in HYR1/GPX3.9 Publications

Miscellaneous

Present with 8000 molecules/cell in log phase SD medium.1 Publication
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide.1 Publication

Caution

Was originally thought to be a glutathione peroxidase (PubMed:10480913) or a phospholipid hydroperoxide glutathione peroxidase (PubMed:11445588), but functions as an atypical 2-Cys peroxiredoxin using thioredoxin as reducing power instead (PubMed:12437921).3 Publications

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36Cysteine sulfenic acid (-SOH) intermediate1 Publication1 Publication1

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: SGD

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciYEAST:YIR037W-MONOMER
ReactomeiR-SCE-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-SCE-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-SCE-3299685 Detoxification of Reactive Oxygen Species
R-SCE-5628897 TP53 Regulates Metabolic Genes
R-SCE-74259 Purine catabolism
R-SCE-9018676 Biosynthesis of D-series resolvins
R-SCE-9018896 Biosynthesis of E-series 18(S)-resolvins
R-SCE-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-SCE-9023661 Biosynthesis of E-series 18(R)-resolvins

Protein family/group databases

PeroxiBasei3742 SceGPx03

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione peroxidase-like peroxiredoxin HYR1Curated (EC:1.11.1.152 Publications)
Alternative name(s):
Glutathione peroxidase homolog 31 Publication
Short name:
GPx 3
Hydrogen peroxide resistance protein 11 Publication
Oxidant receptor peroxidase 11 Publication
Phospholipid hydroperoxide glutathione peroxidase 31 Publication
Short name:
PHGPx3
Gene namesi
Name:HYR11 Publication
Synonyms:GPX31 Publication, ORP11 Publication
Ordered Locus Names:YIR037WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001476 HYR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Peroxisome

Pathology & Biotechi

Disruption phenotypei

Sensitive to hydrogen peroxide and tert-butyl hydroperoxide (t-BHP).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi82C → S: Loss of enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000666431 – 163Glutathione peroxidase-like peroxiredoxin HYR1Add BLAST163

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 82Redox-active1 Publication
Disulfide bondi36Interchain (with C-598 in YAP1); transient2 Publications

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP40581
PaxDbiP40581
PRIDEiP40581
TopDownProteomicsiP40581

PTM databases

iPTMnetiP40581

Expressioni

Inductioni

In contrast to the other two peroxiredoxins, HYR1/GPX3 expression is constitutive, not stress-induced.1 Publication

Interactioni

Subunit structurei

Interacts with YAP1 and probably YBP1.1 Publication

Protein-protein interaction databases

BioGridi35028, 93 interactors
DIPiDIP-1275N
IntActiP40581, 14 interactors
MINTiP40581
STRINGi4932.YIR037W

Structurei

Secondary structure

1163
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP40581
SMRiP40581
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40581

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00760000119230
HOGENOMiHOG000277054
InParanoidiP40581
KOiK00432
OMAiNQFGSQD
OrthoDBiEOG092C4H0P

Family and domain databases

CDDicd00340 GSH_Peroxidase, 1 hit
InterProiView protein in InterPro
IPR000889 Glutathione_peroxidase
IPR029759 GPX_AS
IPR029760 GPX_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR11592 PTHR11592, 1 hit
PfamiView protein in Pfam
PF00255 GSHPx, 1 hit
PIRSFiPIRSF000303 Glutathion_perox, 1 hit
PRINTSiPR01011 GLUTPROXDASE
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00460 GLUTATHIONE_PEROXID_1, 1 hit
PS00763 GLUTATHIONE_PEROXID_2, 1 hit
PS51355 GLUTATHIONE_PEROXID_3, 1 hit

Sequencei

Sequence statusi: Complete.

P40581-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEFYKLAPV DKKGQPFPFD QLKGKVVLIV NVASKCGFTP QYKELEALYK
60 70 80 90 100
RYKDEGFTII GFPCNQFGHQ EPGSDEEIAQ FCQLNYGVTF PIMKKIDVNG
110 120 130 140 150
GNEDPVYKFL KSQKSGMLGL RGIKWNFEKF LVDKKGKVYE RYSSLTKPSS
160
LSETIEELLK EVE
Length:163
Mass (Da):18,641
Last modified:February 1, 1995 - v1
Checksum:i46C42B81E895C1A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22446 Genomic DNA Translation: AAA64283.1
Z38061 Genomic DNA Translation: CAA86197.1
BK006942 Genomic DNA Translation: DAA08584.1
PIRiS48499
RefSeqiNP_012303.1, NM_001179559.1

Genome annotation databases

EnsemblFungiiYIR037W; YIR037W; YIR037W
GeneIDi854855
KEGGisce:YIR037W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22446 Genomic DNA Translation: AAA64283.1
Z38061 Genomic DNA Translation: CAA86197.1
BK006942 Genomic DNA Translation: DAA08584.1
PIRiS48499
RefSeqiNP_012303.1, NM_001179559.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CMIX-ray2.02A1-163[»]
ProteinModelPortaliP40581
SMRiP40581
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35028, 93 interactors
DIPiDIP-1275N
IntActiP40581, 14 interactors
MINTiP40581
STRINGi4932.YIR037W

Protein family/group databases

PeroxiBasei3742 SceGPx03

PTM databases

iPTMnetiP40581

Proteomic databases

MaxQBiP40581
PaxDbiP40581
PRIDEiP40581
TopDownProteomicsiP40581

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR037W; YIR037W; YIR037W
GeneIDi854855
KEGGisce:YIR037W

Organism-specific databases

SGDiS000001476 HYR1

Phylogenomic databases

GeneTreeiENSGT00760000119230
HOGENOMiHOG000277054
InParanoidiP40581
KOiK00432
OMAiNQFGSQD
OrthoDBiEOG092C4H0P

Enzyme and pathway databases

BioCyciYEAST:YIR037W-MONOMER
ReactomeiR-SCE-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-SCE-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-SCE-3299685 Detoxification of Reactive Oxygen Species
R-SCE-5628897 TP53 Regulates Metabolic Genes
R-SCE-74259 Purine catabolism
R-SCE-9018676 Biosynthesis of D-series resolvins
R-SCE-9018896 Biosynthesis of E-series 18(S)-resolvins
R-SCE-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-SCE-9023661 Biosynthesis of E-series 18(R)-resolvins

Miscellaneous databases

EvolutionaryTraceiP40581
PROiPR:P40581

Family and domain databases

CDDicd00340 GSH_Peroxidase, 1 hit
InterProiView protein in InterPro
IPR000889 Glutathione_peroxidase
IPR029759 GPX_AS
IPR029760 GPX_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR11592 PTHR11592, 1 hit
PfamiView protein in Pfam
PF00255 GSHPx, 1 hit
PIRSFiPIRSF000303 Glutathion_perox, 1 hit
PRINTSiPR01011 GLUTPROXDASE
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00460 GLUTATHIONE_PEROXID_1, 1 hit
PS00763 GLUTATHIONE_PEROXID_2, 1 hit
PS51355 GLUTATHIONE_PEROXID_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGPX3_YEAST
AccessioniPrimary (citable) accession number: P40581
Secondary accession number(s): D6VVW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names
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Main funding by: National Institutes of Health

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