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Protein

ATP-dependent DNA helicase MPH1

Gene

MPH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance (PubMed:10880470, PubMed:15126389, PubMed:15634678, PubMed:16121259). Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair (PubMed:19136626). Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork (PubMed:20347428).1 Publication5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 114ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • DNA replication, Okazaki fragment processing Source: SGD
  • donor selection Source: SGD
  • interstrand cross-link repair Source: SGD
  • negative regulation of strand invasion Source: SGD
  • recombinational repair Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31423-MONOMER
BRENDAi3.6.4.12 984
ReactomeiR-SCE-1169408 ISG15 antiviral mechanism
R-SCE-5689880 Ub-specific processing proteases
R-SCE-936440 Negative regulators of DDX58/IFIH1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase MPH1Curated (EC:3.6.4.121 Publication)
Alternative name(s):
FANCM-like protein 1By similarity
Mutator phenotype protein 11 Publication
Gene namesi
Ordered Locus Names:YIR002CImported
ORF Names:YIB2C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001441 MPH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551931 – 993ATP-dependent DNA helicase MPH1Add BLAST993

Proteomic databases

MaxQBiP40562
PaxDbiP40562
PRIDEiP40562

PTM databases

iPTMnetiP40562

Interactioni

Subunit structurei

Interacts with the MHF histone-fold complex composed of MHF1 and MHF2 to form the FANCM-MHF complex.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SMC5Q082045EBI-25369,EBI-34125

Protein-protein interaction databases

BioGridi34993, 262 interactors
DIPiDIP-5016N
IntActiP40562, 9 interactors
STRINGi4932.YIR002C

Structurei

3D structure databases

ProteinModelPortaliP40562
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 261Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST168
Domaini507 – 655Helicase C-terminalPROSITE-ProRule annotationAdd BLAST149

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi209 – 212DEAH boxPROSITE-ProRule annotation4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000046789
HOGENOMiHOG000066122
InParanoidiP40562
KOiK14635
OMAiPEGIKWR
OrthoDBiEOG092C15BQ

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR039686 FANCM/Mph1-like
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PANTHERiPTHR14025:SF20 PTHR14025:SF20, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

P40562-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASADDYFSD FEDDELDKLY EKAINKSVKE TITRRAVPVQ KDLHDNVLPG
60 70 80 90 100
QKTVYEEIQR DVSFGPTHHE LDYDALSFYV YPTNYEVRDY QYTIVHKSLF
110 120 130 140 150
QNTLCAIPTG MGKTFIASTV MLNYFRWTKK AKIIFTAPTR PLVAQQIKAC
160 170 180 190 200
LGITGIPSDQ TAILLDKSRK NREEIWANKR VFFATPQVVE NDLKRGVLDP
210 220 230 240 250
KDIVCLVIDE AHRATGSSAY TNVVKFIDRF NSSYRLLALT ATPASDLEGV
260 270 280 290 300
QEVVNNLDIS KIEIRTEESM DIVKYMKKRK KEKIEVPLLL EIEDIIEQLG
310 320 330 340 350
MAVKPVLQQA IELGIYEECD PSQINAFKAM QQSQKIIANP TIPEGIKWRN
360 370 380 390 400
FFILQLLNNV GQMLKRLKIY GIRTFFNYFQ NKCTEFTTKY NLKKSTNKIA
410 420 430 440 450
AEFYYHPILK NIKNQCENYL SDPKFVGHGK LQCVRDELMD FFQKRGSDSR
460 470 480 490 500
VIIFTELRES ALEIVKFIDS VADDQIRPHI FIGQARAKEG FDEVKYTRKH
510 520 530 540 550
APKGRKKVER LHRQEQEKFL EAERTKRAAN DKLERSARRT GSSEEAQISG
560 570 580 590 600
MNQKMQKEVI HNFKKGEYNV LVCTSIGEEG LDIGEVDLII CYDTTSSPIK
610 620 630 640 650
NIQRMGRTGR KRDGKIVLLF SSNESYKFER AMEDYSTLQA LISKQCIDYK
660 670 680 690 700
KSDRIIPEDI IPECHETLIT INDENEIINE MEDVDEVIRY ATQCMMGKKV
710 720 730 740 750
KPKKAITKKK RVQENKKPKK FFMPDNVETS IVSASTLINK FLVNESGGKQ
760 770 780 790 800
LVTSNENPSK KRKIFKALDN LENDSTEEAS SSLETEDEEV SDDNNVFIAE
810 820 830 840 850
GQNGCQKDLE TAIIRTGESL TTLKPLHNFE RPNMALFVND CGLPTKIEKN
860 870 880 890 900
VKDIRGNQHN LEKEKSCTVD KNNMVLSLDD WNFFRNRYIP EGVSFDVEPN
910 920 930 940 950
FVQYTKGVKV PHCHKVSKII TLFNDESNDN KKRTIDMNYT KCLARGMLRD
960 970 980 990
EKKFVKVNDK SQVDNNSVNH DSSQSFTLSN AELDDILGSD SDF
Length:993
Mass (Da):114,058
Last modified:February 1, 1995 - v1
Checksum:i474DDC99C543171F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA No translation available.
Z38062 Genomic DNA Translation: CAA86204.1
BK006942 Genomic DNA Translation: DAA08548.1
PIRiS48436
RefSeqiNP_012267.1, NM_001179524.1

Genome annotation databases

EnsemblFungiiYIR002C; YIR002C; YIR002C
GeneIDi854818
KEGGisce:YIR002C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79743 Genomic DNA No translation available.
Z38062 Genomic DNA Translation: CAA86204.1
BK006942 Genomic DNA Translation: DAA08548.1
PIRiS48436
RefSeqiNP_012267.1, NM_001179524.1

3D structure databases

ProteinModelPortaliP40562
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34993, 262 interactors
DIPiDIP-5016N
IntActiP40562, 9 interactors
STRINGi4932.YIR002C

PTM databases

iPTMnetiP40562

Proteomic databases

MaxQBiP40562
PaxDbiP40562
PRIDEiP40562

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR002C; YIR002C; YIR002C
GeneIDi854818
KEGGisce:YIR002C

Organism-specific databases

SGDiS000001441 MPH1

Phylogenomic databases

GeneTreeiENSGT00510000046789
HOGENOMiHOG000066122
InParanoidiP40562
KOiK14635
OMAiPEGIKWR
OrthoDBiEOG092C15BQ

Enzyme and pathway databases

BioCyciYEAST:G3O-31423-MONOMER
BRENDAi3.6.4.12 984
ReactomeiR-SCE-1169408 ISG15 antiviral mechanism
R-SCE-5689880 Ub-specific processing proteases
R-SCE-936440 Negative regulators of DDX58/IFIH1 signaling

Miscellaneous databases

PROiPR:P40562

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR039686 FANCM/Mph1-like
IPR006935 Helicase/UvrB_N
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PANTHERiPTHR14025:SF20 PTHR14025:SF20, 1 hit
PfamiView protein in Pfam
PF00271 Helicase_C, 1 hit
PF04851 ResIII, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMPH1_YEAST
AccessioniPrimary (citable) accession number: P40562
Secondary accession number(s): D6VVT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names
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