UniProtKB - P40557 (EPS1_YEAST)
Protein
ER-retained PMA1-suppressing protein 1
Gene
EPS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as a membrane-bound chaperone in endoplasmic reticulum quality control. Probably facilitates presentation of substrate to membrane-bound components of the degradation machinery.3 Publications
Miscellaneous
Present with 6020 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- Catalyzes the rearrangement of -S-S- bonds in proteins. EC:5.3.4.1
GO - Molecular functioni
- protein disulfide isomerase activity Source: SGD
- protein-disulfide reductase (glutathione) activity Source: SGD
- unfolded protein binding Source: SGD
GO - Biological processi
- protein retention in ER lumen Source: SGD
- ubiquitin-dependent ERAD pathway Source: SGD
Keywordsi
Molecular function | Chaperone, Isomerase |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:EPS1 Ordered Locus Names:YIL005W ORF Names:YIA5W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001267, EPS1 |
VEuPathDBi | FungiDB:YIL005W |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 2 Publications; Single-pass membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 646 – 666 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | Sequence analysisAdd BLAST | 27 | |
ChainiPRO_0000034221 | 28 – 701 | ER-retained PMA1-suppressing protein 1Add BLAST | 674 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 60 ↔ 63 | Redox-activePROSITE-ProRule annotation | ||
Glycosylationi | 85 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 200 ↔ 203 | Redox-activePROSITE-ProRule annotation | ||
Glycosylationi | 264 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 299 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 370 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | P40557 |
PaxDbi | P40557 |
PRIDEi | P40557 |
PTM databases
iPTMneti | P40557 |
Interactioni
Subunit structurei
Interacts with mutated PMA1-D378N but not wild type PMA1.
Interacts with EUG1, KAR2, MPD1 AND PDI1.
2 PublicationsGO - Molecular functioni
- unfolded protein binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 34987, 69 interactors |
DIPi | DIP-3860N |
IntActi | P40557, 2 interactors |
MINTi | P40557 |
STRINGi | 4932.YIL005W |
Miscellaneous databases
RNActi | P40557, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 142 | Thioredoxin 1PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 408 – 446 | Thioredoxin 2PROSITE-ProRule annotationAdd BLAST | 39 |
Sequence similaritiesi
Belongs to the protein disulfide isomerase family.Curated
Keywords - Domaini
Redox-active center, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0191, Eukaryota |
HOGENOMi | CLU_024937_0_0_1 |
InParanoidi | P40557 |
OMAi | NAREIMD |
Family and domain databases
Gene3Di | 3.40.30.10, 2 hits |
InterProi | View protein in InterPro IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 2 hits |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P40557-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKMNLKRLVV TFFSCITFLL KFTIAAAEPP EGFPEPLNPT NFKEELSKGL
60 70 80 90 100
HIIDFYSPYC PHCKHLAPVW METWEEFKEE SKTLNITFSQ VNCIESADLC
110 120 130 140 150
GDENIEYFPE IRLYNPSGYI KSFTETPRTK ESLIAFARRE SMDPNNLDTD
160 170 180 190 200
LDSAKSESQY LEGFDFLELI AGKATRPHLV SFWPTKDMKN SDDSLEFKNC
210 220 230 240 250
DKCHEFQRTW KIISRQLAVD DINTGHVNCE SNPTICEELG FGDLVKITNH
260 270 280 290 300
RADREPKVAL VLPNKTSNNL FDYPNGYSAK SDGYVDFARR TFTNSKFPNI
310 320 330 340 350
TEGELEKKAN RDIDFLQERG RVTNNDIHLV FSYDPETVVI EDFDILEYLI
360 370 380 390 400
EPLSKIPNIY LHQIDKNLIN LSRNLFGRMY EKINYDASQT QKVFNKEYFT
410 420 430 440 450
MNTVTQLPTF FMFKDGDPIS YVFPGYSTTE MRNIDAIMDW VKKYSNPLVT
460 470 480 490 500
EVDSSNLKKL ISFQTKSYSD LAIQLISSTD HKHIKGSNKL IKNLLLASWE
510 520 530 540 550
YEHIRMENNF EEINERRARK ADGIKKIKEK KAPANKIVDK MREEIPHMDQ
560 570 580 590 600
KKLLLGYLDI SKEKNFFRKY GITGEYKIGD VIIIDKSNNY YYNKDNFGNS
610 620 630 640 650
LTSNNPQLLR EAFVSLNIPS KALYSSKLKG RLINSPFHNV LSFLDIIHGN
660 670 680 690 700
GMPGYLIVIV LFIAILKGPS IYRRYKVRKH YRAKRNAVGI LGNMEKKKNQ
D
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38113 Genomic DNA Translation: CAA86246.1 AY723829 Genomic DNA Translation: AAU09746.1 X79743 Genomic DNA No translation available. BK006942 Genomic DNA Translation: DAA08541.1 |
PIRi | S48452 |
RefSeqi | NP_012261.1, NM_001179355.1 |
Genome annotation databases
EnsemblFungii | YIL005W_mRNA; YIL005W; YIL005W |
GeneIDi | 854812 |
KEGGi | sce:YIL005W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38113 Genomic DNA Translation: CAA86246.1 AY723829 Genomic DNA Translation: AAU09746.1 X79743 Genomic DNA No translation available. BK006942 Genomic DNA Translation: DAA08541.1 |
PIRi | S48452 |
RefSeqi | NP_012261.1, NM_001179355.1 |
3D structure databases
SMRi | P40557 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34987, 69 interactors |
DIPi | DIP-3860N |
IntActi | P40557, 2 interactors |
MINTi | P40557 |
STRINGi | 4932.YIL005W |
PTM databases
iPTMneti | P40557 |
Proteomic databases
MaxQBi | P40557 |
PaxDbi | P40557 |
PRIDEi | P40557 |
Genome annotation databases
EnsemblFungii | YIL005W_mRNA; YIL005W; YIL005W |
GeneIDi | 854812 |
KEGGi | sce:YIL005W |
Organism-specific databases
SGDi | S000001267, EPS1 |
VEuPathDBi | FungiDB:YIL005W |
Phylogenomic databases
eggNOGi | KOG0191, Eukaryota |
HOGENOMi | CLU_024937_0_0_1 |
InParanoidi | P40557 |
OMAi | NAREIMD |
Miscellaneous databases
PROi | PR:P40557 |
RNActi | P40557, protein |
Family and domain databases
Gene3Di | 3.40.30.10, 2 hits |
InterProi | View protein in InterPro IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 2 hits |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPS1_YEAST | |
Accessioni | P40557Primary (citable) accession number: P40557 Secondary accession number(s): D6VVS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names - SIMILARITY comments
Index of protein domains and families