UniProtKB - P40485 (SLM1_YEAST)
Protein
Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1
Gene
SLM1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Together with SLM2, effector of the TORC2- and calcineurin-signaling pathways. Phosphorylated and activated by TORC2 under favorable growth conditions. Mediates actin polarization via inhibition of calcineurin-dependent transcription. Upon nutrient limitation or environmental stress, gets dephosphorylated by calcineurin. Dephosphorylation inhibits its interaction with TORC2, thereby antagonizing TORC2 signaling and mediating calcineurin-dependent actin depolarization. Also functions in heat-induced, calcineurin-mediated uracil permease (FUR4) endocytosis.4 Publications
Miscellaneous
Present with 5190 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- identical protein binding Source: IntAct
- phosphatidylinositol-4,5-bisphosphate binding Source: SGD
- sphingolipid binding Source: SGD
GO - Biological processi
- actin cytoskeleton organization Source: SGD
- actin filament bundle assembly Source: SGD
- eisosome assembly Source: SGD
- endosomal transport Source: SGD
- establishment or maintenance of actin cytoskeleton polarity Source: SGD
- protein localization to plasma membrane Source: SGD
- regulation of cell growth Source: SGD
- TORC2 signaling Source: InterPro
- TOR signaling Source: SGD
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1Alternative name(s): Synthetic lethal with MSS4 protein 1 TORC2 effector protein SLM1 |
Gene namesi | Name:SLM1 Synonyms:LIT2 Ordered Locus Names:YIL105C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YIL105C |
SGDi | S000001367, SLM1 |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Peripheral membrane protein 3 Publications; Cytoplasmic side 3 Publications
Note: Localizes to cortical punctate structures. Correct localization requires phosphatidylinositol 4,5-bisphosphate and functional TORC2.
Mitochondrion
- mitochondrion Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cytoplasm Source: SGD
- TORC2 complex Source: InterPro
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 477 – 478 | RR → AA in SLM1-PHM2; reduces phosphoinositide binding by 95%; when associated with A-487. 1 Publication | 2 | |
Mutagenesisi | 483 | K → A in SLM1-PHM1; reduces phosphoinositide binding by 80% and causes mislocalization to the cytoplasm; when associated with A-487. 2 Publications | 1 | |
Mutagenesisi | 487 | K → A in SLM1-PHM1; reduces phosphoinositide binding by 80% and causes mislocalization to the cytoplasm; when associated with A-483. In SLM1-PHM2; reduces phosphoinositide binding by 95%; when associated with 477-AA-478. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202965 | 1 – 686 | Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1Add BLAST | 686 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 145 | PhosphoserineCombined sources | 1 | |
Modified residuei | 150 | PhosphoserineCombined sources | 1 | |
Modified residuei | 153 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by the target of rapamycin complex 2 (TORC2) and dephosphorylated by serine/threonine-protein phosphatase 2B (calcineurin).1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P40485 |
PaxDbi | P40485 |
PRIDEi | P40485 |
PTM databases
iPTMneti | P40485 |
Interactioni
Subunit structurei
Heterodimer of SLM1-SLM2. Binds phosphatidylinositol 4,5-bisphosphate, which is required for function.
Interacts with the TORC2 subunits AVO2, BIT61 and TOR2.
Interacts with the calcineurin catalytic subunits CNA1 and CNA2.
4 PublicationsBinary interactionsi
Hide detailsP40485
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-25172,EBI-25172 |
SLM2 [P53955] | 3 | EBI-25172,EBI-28706 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 34886, 151 interactors |
DIPi | DIP-1353N |
ELMi | P40485 |
IntActi | P40485, 27 interactors |
MINTi | P40485 |
STRINGi | 4932.YIL105C |
Miscellaneous databases
RNActi | P40485, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40485 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 468 – 581 | PHPROSITE-ProRule annotationAdd BLAST | 114 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 296 – 381 | Sequence analysisAdd BLAST | 86 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 673 – 678 | PXIXIT-like, required for interaction with CNA1 and CNA2, and calcineurin-dependent dephosphorylation | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 45 – 63 | Poly-GlnAdd BLAST | 19 | |
Compositional biasi | 133 – 136 | Poly-Asn | 4 | |
Compositional biasi | 166 – 172 | Poly-Gln | 7 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502QRAF, Eukaryota |
GeneTreei | ENSGT00940000176324 |
HOGENOMi | CLU_013663_1_0_1 |
InParanoidi | P40485 |
OMAi | WQTFDEW |
Family and domain databases
CDDi | cd13311, PH_Slm1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR033191, Slm1/Slm2 IPR043453, Slm1_PH |
PANTHERi | PTHR31941:SF12, PTHR31941:SF12, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P40485-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKNNTMTSA VSDMLSQQQL NLQHLHNLQQ HTRSMTSADH ANVLQQQQQQ
60 70 80 90 100
QQQQQQQQQQ QQQSASFQNG SLTSDINQQS YLNGQPVPST SNSTFQNNRT
110 120 130 140 150
LTMNSGGLQG IISNGSPNID SNTNVTIAVP DPNNNNGKQL QGKNSLTNTS
160 170 180 190 200
ILSRARSSLQ RQRLAQQQQQ QQDPRSPLVI LVPTAAQPTD ILAARFSAWR
210 220 230 240 250
NVIKSVIVYL TEIASIQDEI VRQQLRLSHA VQFPFFSIEN QYQPSSQEDK
260 270 280 290 300
SVQKFFLPLG NGSIQDLPTI LNQYHESLAS SASKASRELT NDVIPRLEDL
310 320 330 340 350
RRDLIVKIKE IKSLQSDFKN SCSKELQQTK QAMKQFQESL KDARYSVPKQ
360 370 380 390 400
DPFLTKLALD RQIKKQLQEE NFLHEAFDNL ETSGAELEKI VVMEIQNSLT
410 420 430 440 450
IYARLLGQEA QLVFDILISK LDSGFFNVDP QFEWDNFISR DPNFLLPNLP
460 470 480 490 500
MRTFKEIVYK YQFDPLTYEI KSGFLERRSK FLKSYSKGYY VLTPNFLHEF
510 520 530 540 550
KTADRKKDLV PVMSLALSEC TVTEHSRKNS TSSPNSTGSD AKFVLHAKQN
560 570 580 590 600
GIIRRGHNWV FKADSYESMM SWFDNLKILT STSNIQDKYK FITQKLNLNS
610 620 630 640 650
DGKPKLTNNH TSINKYQLSN ANSTMVENDE NDDINSNYVG STVTPKLDNQ
660 670 680
TNTNTSMSSL PDTNDSELQD QVPNIYIQTP INDFKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38125 Genomic DNA Translation: CAA86275.1 BK006942 Genomic DNA Translation: DAA08448.1 |
PIRi | S48467 |
RefSeqi | NP_012161.1, NM_001179453.1 |
Genome annotation databases
EnsemblFungii | YIL105C_mRNA; YIL105C; YIL105C |
GeneIDi | 854701 |
KEGGi | sce:YIL105C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38125 Genomic DNA Translation: CAA86275.1 BK006942 Genomic DNA Translation: DAA08448.1 |
PIRi | S48467 |
RefSeqi | NP_012161.1, NM_001179453.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3NSU | X-ray | 2.00 | A/B | 469-583 | [»] | |
4A5K | X-ray | 1.76 | A/B/C/D | 469-583 | [»] | |
4A6F | X-ray | 1.68 | A/B | 469-583 | [»] | |
4A6H | X-ray | 1.45 | A/B/C/D | 469-583 | [»] | |
4A6K | X-ray | 1.80 | A/B/C/D | 469-583 | [»] | |
SMRi | P40485 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34886, 151 interactors |
DIPi | DIP-1353N |
ELMi | P40485 |
IntActi | P40485, 27 interactors |
MINTi | P40485 |
STRINGi | 4932.YIL105C |
PTM databases
iPTMneti | P40485 |
Proteomic databases
MaxQBi | P40485 |
PaxDbi | P40485 |
PRIDEi | P40485 |
Genome annotation databases
EnsemblFungii | YIL105C_mRNA; YIL105C; YIL105C |
GeneIDi | 854701 |
KEGGi | sce:YIL105C |
Organism-specific databases
EuPathDBi | FungiDB:YIL105C |
SGDi | S000001367, SLM1 |
Phylogenomic databases
eggNOGi | ENOG502QRAF, Eukaryota |
GeneTreei | ENSGT00940000176324 |
HOGENOMi | CLU_013663_1_0_1 |
InParanoidi | P40485 |
OMAi | WQTFDEW |
Miscellaneous databases
PROi | PR:P40485 |
RNActi | P40485, protein |
Family and domain databases
CDDi | cd13311, PH_Slm1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR033191, Slm1/Slm2 IPR043453, Slm1_PH |
PANTHERi | PTHR31941:SF12, PTHR31941:SF12, 1 hit |
Pfami | View protein in Pfam PF00169, PH, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SLM1_YEAST | |
Accessioni | P40485Primary (citable) accession number: P40485 Secondary accession number(s): D6VVI2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | October 7, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names