UniProtKB - P40477 (NU159_YEAST)
Nucleoporin NUP159
NUP159
Functioni
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.
13 PublicationsMiscellaneous
GO - Molecular functioni
- adenyl-nucleotide exchange factor activity Source: SGD
- structural constituent of nuclear pore Source: SGD
- transcription corepressor activity Source: GO_Central
GO - Biological processi
- ncRNA export from nucleus Source: SGD
- NLS-bearing protein import into nucleus Source: SGD
- nuclear pore localization Source: SGD
- nucleocytoplasmic transport Source: ComplexPortal
- poly(A)+ mRNA export from nucleus Source: SGD
- protein export from nucleus Source: SGD
- ribosomal large subunit export from nucleus Source: SGD
- ribosomal small subunit export from nucleus Source: SGD
- selective autophagy Source: SGD
Keywordsi
Biological process | mRNA transport, Protein transport, Translocation, Transport |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoporin NUP159Alternative name(s): Nuclear pore protein NUP159 |
Gene namesi | Name:NUP159 Synonyms:NUP158, RAT7 Ordered Locus Names:YIL115C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001377, NUP159 |
VEuPathDBi | FungiDB:YIL115C |
Subcellular locationi
Nucleus
- nuclear pore complex 4 Publications
- Nucleus membrane ; Peripheral membrane protein ; Cytoplasmic side
Nucleus
- nuclear envelope Source: ComplexPortal
- nuclear membrane Source: UniProtKB-SubCell
- nuclear pore Source: SGD
- nuclear pore central transport channel Source: SGD
- nuclear pore cytoplasmic filaments Source: SGD
- nucleus Source: GO_Central
Keywords - Cellular componenti
Membrane, Nuclear pore complex, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204849 | 1 – 1460 | Nucleoporin NUP159Add BLAST | 1460 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 404 | PhosphoserineCombined sources | 1 | |
Modified residuei | 657 | PhosphoserineCombined sources | 1 | |
Modified residuei | 724 | PhosphoserineCombined sources | 1 | |
Modified residuei | 735 | PhosphoserineCombined sources | 1 | |
Modified residuei | 745 | PhosphoserineCombined sources | 1 | |
Modified residuei | 803 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 805 | PhosphoserineCombined sources | 1 | |
Modified residuei | 819 | PhosphoserineCombined sources | 1 | |
Modified residuei | 889 | PhosphoserineCombined sources | 1 | |
Modified residuei | 940 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P40477 |
PaxDbi | P40477 |
PRIDEi | P40477 |
PTM databases
iPTMneti | P40477 |
Interactioni
Subunit structurei
Component of the nuclear pore complex (NPC) (PubMed:17546040). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Part of the NUP82 subcomplex, interacts with NUP82 through its C-terminal coiled coil (PubMed:9736720, PubMed:17546040, PubMed:23223634, PubMed:25646085). This subcomplex is the base for interactions with NUP116 and GLE2, with NUP42 and GLE1 and with DYN2 (PubMed:17546040, PubMed:23223634).
Interacts directly with DYN2 (PubMed:17546040, PubMed:23223634, PubMed:25646085).
Interacts through its FG repeats with karyopherins, such as heterodimeric mRNA transport factor MEX67/MTR2, CRM1 (XPO1), and PSE1 (GSP1-GDP dependent). Interaction with CRM1 (XPO1) is GSP1-GTP dependent and stimulated by RNA1. NUP159 also interacts with GLE1 and the ATP-dependent RNA helicase DBP5.
13 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 34876, 245 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-2314N |
IntActi | P40477, 18 interactors |
MINTi | P40477 |
STRINGi | 4932.YIL115C |
Miscellaneous databases
RNActi | P40477, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P40477 |
SMRi | P40477 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40477 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 228 – 231 | FG 1 | 4 | |
Repeati | 267 – 270 | PXFG 1 | 4 | |
Repeati | 462 – 470 | SXFGXPXFG 1 | 9 | |
Repeati | 503 – 511 | SXFGXPXFG 2; approximate | 9 | |
Repeati | 522 – 530 | SXFGXPXFG 3; approximate | 9 | |
Repeati | 532 – 535 | PXFG 2 | 4 | |
Repeati | 548 – 556 | SXFGXPXFG 4 | 9 | |
Repeati | 558 – 561 | PXFG 3 | 4 | |
Repeati | 574 – 582 | SXFGXPXFG 5 | 9 | |
Repeati | 584 – 587 | PXFG 4 | 4 | |
Repeati | 600 – 608 | SXFGXPXFG 6 | 9 | |
Repeati | 610 – 613 | SXFG 1 | 4 | |
Repeati | 624 – 632 | SXFGXPXFG 7; approximate | 9 | |
Repeati | 642 – 645 | FG 2 | 4 | |
Repeati | 687 – 690 | FG 3 | 4 | |
Repeati | 704 – 707 | FXFG 1 | 4 | |
Repeati | 709 – 712 | SXFG 2 | 4 | |
Repeati | 728 – 731 | FXFG 2 | 4 | |
Repeati | 842 – 845 | PXFG 5 | 4 | |
Repeati | 873 – 876 | FXFG 3 | 4 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 500 | Interaction with DBP5Add BLAST | 500 | |
Regioni | 401 – 435 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 483 – 504 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 497 – 701 | Interactions with CRM1 and GLE1Add BLAST | 205 | |
Regioni | 533 – 619 | DisorderedSequence analysisAdd BLAST | 87 | |
Regioni | 647 – 704 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 727 – 824 | DisorderedSequence analysisAdd BLAST | 98 | |
Regioni | 861 – 1092 | DisorderedSequence analysisAdd BLAST | 232 | |
Regioni | 1086 – 1175 | Interaction with DYN22 PublicationsAdd BLAST | 90 | |
Regioni | 1223 – 1460 | Interaction with NUP821 PublicationAdd BLAST | 238 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1279 – 1320 | Add BLAST | 42 | |
Coiled coili | 1383 – 1418 | Add BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 413 – 427 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 483 – 497 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 533 – 571 | Polar residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 578 – 619 | Polar residuesSequence analysisAdd BLAST | 42 | |
Compositional biasi | 647 – 673 | Polar residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 683 – 704 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 727 – 766 | Polar residuesSequence analysisAdd BLAST | 40 | |
Compositional biasi | 778 – 802 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 861 – 893 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 914 – 937 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 942 – 963 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 964 – 999 | Basic and acidic residuesSequence analysisAdd BLAST | 36 | |
Compositional biasi | 1016 – 1092 | Basic and acidic residuesSequence analysisAdd BLAST | 77 |
Domaini
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | KOG3630, Eukaryota |
HOGENOMi | CLU_250354_0_0_1 |
InParanoidi | P40477 |
OMAi | QAFENDE |
Family and domain databases
DisProti | DP01078 |
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR039462, Nup159/Nup146_N IPR015943, WD40/YVTN_repeat-like_dom_sf |
Pfami | View protein in Pfam PF16755, NUP214, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSSLKDEVPT ETSEDFGFKF LGQKQILPSF NEKLPFASLQ NLDISNSKSL
60 70 80 90 100
FVAASGSKAV VGELQLLRDH ITSDSTPLTF KWEKEIPDVI FVCFHGDQVL
110 120 130 140 150
VSTRNALYSL DLEELSEFRT VTSFEKPVFQ LKNVNNTLVI LNSVNDLSAL
160 170 180 190 200
DLRTKSTKQL AQNVTSFDVT NSQLAVLLKD RSFQSFAWRN GEMEKQFEFS
210 220 230 240 250
LPSELEELPV EEYSPLSVTI LSPQDFLAVF GNVISETDDE VSYDQKMYII
260 270 280 290 300
KHIDGSASFQ ETFDITPPFG QIVRFPYMYK VTLSGLIEPD ANVNVLASSC
310 320 330 340 350
SSEVSIWDSK QVIEPSQDSE RAVLPISEET DKDTNPIGVA VDVVTSGTIL
360 370 380 390 400
EPCSGVDTIE RLPLVYILNN EGSLQIVGLF HVAAIKSGHY SINLESLEHE
410 420 430 440 450
KSLSPTSEKI PIAGQEQEEK KKNNESSKAL SENPFTSANT SGFTFLKTQP
460 470 480 490 500
AAANSLQSQS SSTFGAPSFG SSAFKIDLPS VSSTSTGVAS SEQDATDPAS
510 520 530 540 550
AKPVFGKPAF GAIAKEPSTS EYAFGKPSFG APSFGSGKSS VESPASGSAF
560 570 580 590 600
GKPSFGTPSF GSGNSSVEPP ASGSAFGKPS FGTPSFGSGN SSAEPPASGS
610 620 630 640 650
AFGKPSFGTS AFGTASSNET NSGSIFGKAA FGSSSFAPAN NELFGSNFTI
660 670 680 690 700
SKPTVDSPKE VDSTSPFPSS GDQSEDESKS DVDSSSTPFG TKPNTSTKPK
710 720 730 740 750
TNAFDFGSSS FGSGFSKALE SVGSDTTFKF GTQASPFSSQ LGNKSPFSSF
760 770 780 790 800
TKDDTENGSL SKGSTSEIND DNEEHESNGP NVSGNDLTDS TVEQTSSTRL
810 820 830 840 850
PETPSDEDGE VVEEEAQKSP IGKLTETIKK SANIDMAGLK NPVFGNHVKA
860 870 880 890 900
KSESPFSAFA TNITKPSSTT PAFSFGNSTM NKSNTSTVSP MEEADTKETS
910 920 930 940 950
EKGPITLKSV ENPFLPAKEE RTGESSKKDH NDDPKDGYVS GSEISVRTSE
960 970 980 990 1000
SAFDTTANEE IPKSQDVNNH EKSETDPKYS QHAVVDHDNK SKEMNETSKN
1010 1020 1030 1040 1050
NERSGQPNHG VQGDGIALKK DNEKENFDSN MAIKQFEDHQ SSEEDASEKD
1060 1070 1080 1090 1100
SRQSSEVKES DDNMSLNSDR DESISESYDK LEDINTDELP HGGEAFKARE
1110 1120 1130 1140 1150
VSASADFDVQ TSLEDNYAES GIQTDLSESS KENEVQTDAI PVKHNSTQTV
1160 1170 1180 1190 1200
KKEAVDNGLQ TEPVETCNFS VQTFEGDENY LAEQCKPKQL KEYYTSAKVS
1210 1220 1230 1240 1250
NIPFVSQNST LRLIESTFQT VEAEFTVLME NIRNMDTFFT DQSSIPLVKR
1260 1270 1280 1290 1300
TVRSINNLYT WRIPEAEILL NIQNNIKCEQ MQITNANIQD LKEKVTDYVR
1310 1320 1330 1340 1350
KDIAQITEDV ANAKEEYLFL MHFDDASSGY VKDLSTHQFR MQKTLRQKLF
1360 1370 1380 1390 1400
DVSAKINHTE ELLNILKLFT VKNKRLDDNP LVAKLAKESL ARDGLLKEIK
1410 1420 1430 1440 1450
LLREQVSRLQ LEEKGKKASS FDASSSITKD MKGFKVVEVG LAMNTKKQIG
1460
DFFKNLNMAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L40634 Genomic DNA Translation: AAC41652.1 Z38125 Genomic DNA Translation: CAA86265.1 BK006942 Genomic DNA Translation: DAA08438.1 |
PIRi | S48457 |
RefSeqi | NP_012151.1, NM_001179463.1 |
Genome annotation databases
EnsemblFungii | YIL115C_mRNA; YIL115C; YIL115C |
GeneIDi | 854691 |
KEGGi | sce:YIL115C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L40634 Genomic DNA Translation: AAC41652.1 Z38125 Genomic DNA Translation: CAA86265.1 BK006942 Genomic DNA Translation: DAA08438.1 |
PIRi | S48457 |
RefSeqi | NP_012151.1, NM_001179463.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XIP | X-ray | 2.50 | A | 2-387 | [»] | |
3PBP | X-ray | 2.60 | C/F/I/L | 1425-1460 | [»] | |
3RRM | X-ray | 2.88 | C | 2-387 | [»] | |
3TKN | X-ray | 3.40 | B/E/H | 1425-1460 | [»] | |
4DS1 | X-ray | 1.85 | B/D | 1116-1126 | [»] | |
7N9F | electron microscopy | 37.00 | w/x | 1-1460 | [»] | |
AlphaFoldDBi | P40477 | |||||
SMRi | P40477 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34876, 245 interactors |
ComplexPortali | CPX-824, Nuclear pore complex |
DIPi | DIP-2314N |
IntActi | P40477, 18 interactors |
MINTi | P40477 |
STRINGi | 4932.YIL115C |
Protein family/group databases
TCDBi | 1.I.1.1.1, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | P40477 |
Proteomic databases
MaxQBi | P40477 |
PaxDbi | P40477 |
PRIDEi | P40477 |
Protocols and materials databases
DNASUi | 854691 |
Genome annotation databases
EnsemblFungii | YIL115C_mRNA; YIL115C; YIL115C |
GeneIDi | 854691 |
KEGGi | sce:YIL115C |
Organism-specific databases
SGDi | S000001377, NUP159 |
VEuPathDBi | FungiDB:YIL115C |
Phylogenomic databases
eggNOGi | KOG3630, Eukaryota |
HOGENOMi | CLU_250354_0_0_1 |
InParanoidi | P40477 |
OMAi | QAFENDE |
Miscellaneous databases
EvolutionaryTracei | P40477 |
PROi | PR:P40477 |
RNActi | P40477, protein |
Family and domain databases
DisProti | DP01078 |
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR039462, Nup159/Nup146_N IPR015943, WD40/YVTN_repeat-like_dom_sf |
Pfami | View protein in Pfam PF16755, NUP214, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NU159_YEAST | |
Accessioni | P40477Primary (citable) accession number: P40477 Secondary accession number(s): D6VVH2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references