UniProtKB - P40466 (FKH1_YEAST)
Protein
Fork head protein homolog 1
Gene
FKH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transcription factor that regulates the expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle (PubMed:10959837, PubMed:10894548, PubMed:10894549, PubMed:11562353, PubMed:12702877, PubMed:17283050, PubMed:24504085). The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20 as well as SWI5 and ACE2, transcription factors required for the subsequent temporal wave of cell cycle regulated gene expression in the M/G1 phase interval (PubMed:10959837, PubMed:10894548, PubMed:11562353). Involved in HMRa silencing (PubMed:10747051). FKH1 and FKH2 associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination, and coordinate early transcription elongation and pre-mRNA processing (PubMed:12702877). Both FKH1 and FKH2 play a role as regulators of lifespan in collaboration with the anaphase-promoting complex (APC), likely through combined regulation of stress response, genomic stability, and cell cycle regulation (PubMed:22438832). FKH1 and FKH2 function also in controlling yeast cell morphology by preventing preudohyphal growth (PubMed:10747051, PubMed:10894548). Acts as a rate-limiting replication origin activator via its interactin with the origin recognition complex (ORC) (PubMed:22265405, PubMed:26728715). Plays a transcription-independent role in recombination donor preference during mating-type switching through binding to the recombination enhancer (RE), a 700-bp cis-acting element that controls recombination along the left arm of chromosome III (PubMed:12183363, PubMed:16809780, PubMed:22496671, PubMed:27257873).15 Publications
Miscellaneous
Present with 1720 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 302 – 393 | Fork-headPROSITE-ProRule annotationAdd BLAST | 92 |
GO - Molecular functioni
- centromeric DNA binding Source: SGD
- chromatin binding Source: SGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: SGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: SGD
- DNA replication origin binding Source: SGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: SGD
- sequence-specific DNA binding Source: SGD
GO - Biological processi
- chromatin remodeling Source: SGD
- donor selection Source: SGD
- mRNA 3'-end processing Source: SGD
- negative regulation of pseudohyphal growth Source: SGD
- negative regulation of transcription elongation from RNA polymerase II promoter Source: SGD
- negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
- negative regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
- positive regulation of DNA-dependent DNA replication initiation Source: SGD
- positive regulation of DNA replication initiation Source: SGD
- positive regulation of silent mating-type cassette heterochromatin assembly Source: SGD
- positive regulation of transcription involved in G2/M transition of mitotic cell cycle Source: SGD
- regulation of transcription by RNA polymerase II Source: GO_Central
- termination of RNA polymerase II transcription Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Fork head protein homolog 11 Publication |
Gene namesi | Name:FKH11 Publication Ordered Locus Names:YIL131C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001393, FKH1 |
VEuPathDBi | FungiDB:YIL131C |
Pathology & Biotechi
Disruption phenotypei
Causes only a modest decline in CLB2 cluster genes expression, but this expression is abolished when both FKH1 and FKH2 are deleted (PubMed:10959837). Leads to a defect in silencing HMRa (PubMed:10747051). Causes a form of yeast pseudohyphal growth, when FKH2 is also deleted (PubMed:10747051). Affects cell-cycle progression and CLB2 mRNA expression (PubMed:10747051). Leads to defect in pre-mRNA 3' end formation during transcription of targeted genes (PubMed:12702877).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 80 | R → A: Leads to reduced colony size when FKH2 is deleted. 1 Publication | 1 | |
Mutagenesisi | 110 | S → A: Leads to reduced colony size when FKH2 is deleted. 1 Publication | 1 | |
Mutagenesisi | 111 | R → A: Leads to reduced colony size when FKH2 is deleted. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091903 | 1 – 484 | Fork head protein homolog 1Add BLAST | 484 |
Proteomic databases
MaxQBi | P40466 |
PaxDbi | P40466 |
PRIDEi | P40466 |
PTM databases
iPTMneti | P40466 |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 34860, 131 interactors |
DIPi | DIP-5220N |
IntActi | P40466, 34 interactors |
STRINGi | 4932.YIL131C |
Miscellaneous databases
RNActi | P40466, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 76 – 142 | FHAPROSITE-ProRule annotationAdd BLAST | 67 |
Domaini
The phosphothreonine-binding FHA domain is required for the interaction with MPH1 and controlling recombination donor preference during mating-type switching.1 Publication
Phylogenomic databases
eggNOGi | KOG2294, Eukaryota |
GeneTreei | ENSGT00940000173882 |
HOGENOMi | CLU_044386_0_0_1 |
InParanoidi | P40466 |
OMAi | WIADHYA |
Family and domain databases
CDDi | cd00059, FH, 1 hit cd00060, FHA, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000253, FHA_dom IPR001766, Fork_head_dom IPR008984, SMAD_FHA_dom_sf IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00498, FHA, 1 hit PF00250, Forkhead, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit SM00240, FHA, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS50006, FHA_DOMAIN, 1 hit PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P40466-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVTSREQKF SGKYSSYTAQ DRQGLVNAVT CVLSSSSDPV AVSSDYSNSL
60 70 80 90 100
SIAREVNAYA KIAGCDWTYY VQKLEVTIGR NTDSLNLNAV PGTVVKKNID
110 120 130 140 150
IDLGPAKIVS RKHAAIRFNL ESGSWELQIF GRNGAKVNFR RIPTGPDSPP
160 170 180 190 200
TVLQSGCIID IGGVQMIFIL PEQETIISDY CLNHLMPKLL STYGTNGNNN
210 220 230 240 250
PLLRNIIEGS TYLREQRLQE EARLQQLDHL HTPLSSSSDV NPIGDPHGDT
260 270 280 290 300
IMMEEDEEDE NYTRGGIRPN TYTSSSNNAV TNGNVPHIEN PSDLSLDENR
310 320 330 340 350
YIKPPQSYAS MITQAILSTP EGSISLADIY KFISDNYAFY RFSQMAWQNS
360 370 380 390 400
VRHNLSLNKA FEKVPKRAGQ QGKGMNWKIS DEVRRDFLNK WNAGKLSKIR
410 420 430 440 450
RGASVTRQLQ LHMSKFGEIP APESSSIDPR GIKAQKVKKS LQATSSILGE
460 470 480
SAPQLQRTQL TGQISTTTSM DVTTNANVNN SSLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38848 Genomic DNA Translation: AAA60938.1 DQ115392 Genomic DNA Translation: AAZ22496.1 Z38059 Genomic DNA Translation: CAA86147.1 BK006942 Genomic DNA Translation: DAA08422.1 |
PIRi | S48403 |
RefSeqi | NP_012135.1, NM_001179479.1 |
Genome annotation databases
EnsemblFungii | YIL131C_mRNA; YIL131C; YIL131C |
GeneIDi | 854675 |
KEGGi | sce:YIL131C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L38848 Genomic DNA Translation: AAA60938.1 DQ115392 Genomic DNA Translation: AAZ22496.1 Z38059 Genomic DNA Translation: CAA86147.1 BK006942 Genomic DNA Translation: DAA08422.1 |
PIRi | S48403 |
RefSeqi | NP_012135.1, NM_001179479.1 |
3D structure databases
SMRi | P40466 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34860, 131 interactors |
DIPi | DIP-5220N |
IntActi | P40466, 34 interactors |
STRINGi | 4932.YIL131C |
PTM databases
iPTMneti | P40466 |
Proteomic databases
MaxQBi | P40466 |
PaxDbi | P40466 |
PRIDEi | P40466 |
Genome annotation databases
EnsemblFungii | YIL131C_mRNA; YIL131C; YIL131C |
GeneIDi | 854675 |
KEGGi | sce:YIL131C |
Organism-specific databases
SGDi | S000001393, FKH1 |
VEuPathDBi | FungiDB:YIL131C |
Phylogenomic databases
eggNOGi | KOG2294, Eukaryota |
GeneTreei | ENSGT00940000173882 |
HOGENOMi | CLU_044386_0_0_1 |
InParanoidi | P40466 |
OMAi | WIADHYA |
Miscellaneous databases
PROi | PR:P40466 |
RNActi | P40466, protein |
Family and domain databases
CDDi | cd00059, FH, 1 hit cd00060, FHA, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR000253, FHA_dom IPR001766, Fork_head_dom IPR008984, SMAD_FHA_dom_sf IPR018122, TF_fork_head_CS_1 IPR030456, TF_fork_head_CS_2 IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00498, FHA, 1 hit PF00250, Forkhead, 1 hit |
PRINTSi | PR00053, FORKHEAD |
SMARTi | View protein in SMART SM00339, FH, 1 hit SM00240, FHA, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF49879, SSF49879, 1 hit |
PROSITEi | View protein in PROSITE PS50006, FHA_DOMAIN, 1 hit PS00657, FORK_HEAD_1, 1 hit PS00658, FORK_HEAD_2, 1 hit PS50039, FORK_HEAD_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FKH1_YEAST | |
Accessioni | P40466Primary (citable) accession number: P40466 Secondary accession number(s): D6VVF6, Q45U14 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names