ATG32

Functionⁱ

Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation.9 Publications

GO - Biological processⁱ

autophagy of mitochondrion
Source: SGD
Inferred from mutant phenotypeⁱ
- 19619494
- 19619495

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Autophagy

Biological process

Autophagy

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31395-MONOMER

BioCycⁱ

YEAST:G3O-31395-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Autophagy-related protein 32 Alternative name(s): Extracellular mutant protein 37
Gene namesⁱ	Name:ATG32 Synonyms:ECM17 Ordered Locus Names:YIL146C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome IX

Organism-specific databases

EuPathDBⁱ	FungiDB:YIL146C
Saccharomyces Genome Database More...SGDⁱ	S000001408 ATG32

Topology

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Transmembraneⁱ	390 – 414	HelicalSequence analysisAdd BLAST		25

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion outer membrane, Vacuole

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	114	S → A, N, G or Y: Abolishes mitophagy and impairs interaction with ATG11. 1 Publication		1
Mutagenesisⁱ	119	S → A: Decreases mitophagy and impairs interaction with ATG11. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086920	1 – 529	Autophagy-related protein 32Add BLAST		529

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	114	Phosphoserine1 Publication		1
Modified residueⁱ	119	Phosphoserine1 Publication		1

Post-translational modificationⁱ

Phosphorylation of Ser-114 and Ser-119 are critically important for mitophagy and for the ATG11-ATG32 interaction. Phosphorylation depends on both HOG1 and PBS2.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P40458
PaxDbⁱ	P40458
PRIDEⁱ	P40458

PTM databases

iPTMnetⁱ	P40458

iPTMnetⁱ

P40458

Interactionⁱ

Subunit structureⁱ

interacts with ATG8 and ATG11.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ATG11	Q12527	2	EBI-25256,EBI-31977
CKA1	P15790	2	EBI-25256,EBI-9533

Show more details

Protein-protein interaction databases

BioGridⁱ	34846, 241 interactors
IntActⁱ	P40458, 6 interactors
Molecular INTeraction database More...MINTⁱ	P40458
STRINGⁱ	4932.YIL146C

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3VXW	X-ray	3.00	B	85-90	[»]
ProteinModelPortalⁱ	P40458
SMRⁱ	P40458
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ATG32 family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidⁱ	P40458
KEGG Orthology (KO) More...KOⁱ	K17984
OMAⁱ	NDINTHT
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2FBN

Sequenceⁱ

Sequence statusⁱ: Complete.

P40458-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVLEYQQREG KGSSSKSMPP DSSSTTIHTC SEAQTGEDKG LLDPHLSVLE 
        60         70         80         90        100
LLSKTGHSPS PMGQNLVTSI DISGNHNVND SISGSWQAIQ PLDLGASFIP 
       110        120        130        140        150
ERCSSQTTNG SILSSSDTSE EEQELLQAPA ADIINIIKQG QEGANVVSPS 
       160        170        180        190        200
HPFKQLQKII SLPLPGKEKT PFNEQDDDGD EDEAFEEDSV TITKSLTSST 
       210        220        230        240        250
NSFVMPKLSL TQKNPVFRLL ILGRTGSSFY QSIPKEYQSL FELPKYHDSA 
       260        270        280        290        300
TFPQYTGIVI IFQELREMVS LLNRIVQYSQ GKPVIPICQP GQVIQVKNVL 
       310        320        330        340        350
KSFLRNKLVK LLFPPVVVTN KRDLKKMFQR LQDLSLEYGE DVNEEDNDDE 
       360        370        380        390        400
AIHTKSRSYC RNKKAENSKK KSPKSNKKPK RKKQKFFTSW FTWGISITIG 
       410        420        430        440        450
ISFGCCVTYF VTAAYEHQTV KSLSLRPSIL ASLLSLDSSS DTINTPATAS 
       460        470        480        490        500
PSSTEQFLWF DKGTLQINFH SDGFIMKSLT IIKETWGKMN TFVLHALSKP 
       510        520 
LKFLENLNKS SEFSIDESNR ILALGYILL

Length:529

Mass (Da):58,969

Last modified:February 1, 1995 - v1

Checksum:ⁱDB6A3DAFD50FB619

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z38059 Genomic DNA Translation: CAA86132.1 AY692933 Genomic DNA Translation: AAT92952.1 BK006942 Genomic DNA Translation: DAA08407.1
PIRⁱ	S48388
NCBI Reference Sequences More...RefSeqⁱ	NP_012120.1, NM_001179494.1

Genome annotation databases

EnsemblFungiⁱ	YIL146C; YIL146C; YIL146C
GeneIDⁱ	854660
KEGGⁱ	sce:YIL146C

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P40458	Autophagy-related protein 32		YEAS2		529
Autophagy-related protein 32		YEAS6		529
Autophagy-related protein 32		YEAS7		529
Autophagy-related protein 32		YEAS8		529
ATG32p Mitochondrial outer membrane protein required to initiate mitophagy		Saccharomyces sp. 'boulardii'		529
+4

Protein	Similar proteins		Species	Score	Length
P40458	Autophagy-related protein 32		YEAS2		529
Autophagy-related protein 32		YEAS6		529
Autophagy-related protein 32		YEAS7		529
Autophagy-related protein 32		YEAS8		529
ATG32p Mitochondrial outer membrane protein required to initiate mitophagy		Saccharomyces sp. 'boulardii'		529
+8

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z38059 Genomic DNA Translation: CAA86132.1 AY692933 Genomic DNA Translation: AAT92952.1 BK006942 Genomic DNA Translation: DAA08407.1
PIRⁱ	S48388
NCBI Reference Sequences More...RefSeqⁱ	NP_012120.1, NM_001179494.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3VXW	X-ray	3.00	B	85-90	[»]
ProteinModelPortalⁱ	P40458
SMRⁱ	P40458
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	34846, 241 interactors
IntActⁱ	P40458, 6 interactors
Molecular INTeraction database More...MINTⁱ	P40458
STRINGⁱ	4932.YIL146C

PTM databases

iPTMnetⁱ	P40458

iPTMnetⁱ

P40458

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P40458
PaxDbⁱ	P40458
PRIDEⁱ	P40458

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YIL146C; YIL146C; YIL146C
GeneIDⁱ	854660
KEGGⁱ	sce:YIL146C

Organism-specific databases

EuPathDBⁱ	FungiDB:YIL146C
Saccharomyces Genome Database More...SGDⁱ	S000001408 ATG32

Phylogenomic databases

InParanoidⁱ	P40458
KEGG Orthology (KO) More...KOⁱ	K17984
OMAⁱ	NDINTHT
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2FBN

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31395-MONOMER

BioCycⁱ

YEAST:G3O-31395-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P40458

PROⁱ

PR:P40458

Family and domain databases

ProtoNetⁱ	Search...

ProtoNetⁱ

Search...

Entry informationⁱ

Entry nameⁱ	ATG32_YEAST
Accessionⁱ	P40458Primary (citable) accession number: P40458 Secondary accession number(s): D6VVE1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
	Last sequence update:	February 1, 1995
	Last modified:	May 23, 2018
	This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

UniProtKB

UniParc

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Supporting data

UniProtKB - P40458 (ATG32_YEAST)

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Autophagy-related protein 32

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Vacuole

Mitochondrion

Other locations

Mitochondrion

Vacuole

Other locations

Topology

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ