UniProtKB - P40458 (ATG32_YEAST)
Protein
Autophagy-related protein 32
Gene
ATG32
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation.9 Publications
GO - Biological processi
- autophagy of mitochondrion Source: SGD
- mitochondria-nucleus signaling pathway Source: SGD
Keywordsi
Biological process | Autophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Autophagy-related protein 32Alternative name(s): Extracellular mutant protein 37 |
Gene namesi | Name:ATG32 Synonyms:ECM17 Ordered Locus Names:YIL146C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001408, ATG32 |
VEuPathDBi | FungiDB:YIL146C |
Subcellular locationi
Mitochondrion
Vacuole
Other locations
Note: Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation.
Mitochondrion
- integral component of mitochondrial outer membrane Source: SGD
- mitochondrion Source: SGD
Vacuole
- vacuolar membrane Source: UniProtKB-SubCell
Other locations
- phagophore assembly site membrane Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 390 – 414 | HelicalSequence analysisAdd BLAST | 25 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membrane, VacuolePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 114 | S → A, N, G or Y: Abolishes mitophagy and impairs interaction with ATG11. 1 Publication | 1 | |
Mutagenesisi | 119 | S → A: Decreases mitophagy and impairs interaction with ATG11. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086920 | 1 – 529 | Autophagy-related protein 32Add BLAST | 529 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 114 | Phosphoserine1 Publication | 1 | |
Modified residuei | 119 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Phosphorylation of Ser-114 and Ser-119 are critically important for mitophagy and for the ATG11-ATG32 interaction. Phosphorylation depends on both HOG1 and PBS2.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P40458 |
PaxDbi | P40458 |
PRIDEi | P40458 |
PTM databases
iPTMneti | P40458 |
Interactioni
Subunit structurei
interacts with ATG8 and ATG11.
4 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 34846, 242 interactors |
IntActi | P40458, 6 interactors |
MINTi | P40458 |
STRINGi | 4932.YIL146C |
Miscellaneous databases
RNActi | P40458, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40458 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATG32 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QY5V, Eukaryota |
HOGENOMi | CLU_039418_0_0_1 |
InParanoidi | P40458 |
OMAi | IPICQPG |
i Sequence
Sequence statusi: Complete.
P40458-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLEYQQREG KGSSSKSMPP DSSSTTIHTC SEAQTGEDKG LLDPHLSVLE
60 70 80 90 100
LLSKTGHSPS PMGQNLVTSI DISGNHNVND SISGSWQAIQ PLDLGASFIP
110 120 130 140 150
ERCSSQTTNG SILSSSDTSE EEQELLQAPA ADIINIIKQG QEGANVVSPS
160 170 180 190 200
HPFKQLQKII SLPLPGKEKT PFNEQDDDGD EDEAFEEDSV TITKSLTSST
210 220 230 240 250
NSFVMPKLSL TQKNPVFRLL ILGRTGSSFY QSIPKEYQSL FELPKYHDSA
260 270 280 290 300
TFPQYTGIVI IFQELREMVS LLNRIVQYSQ GKPVIPICQP GQVIQVKNVL
310 320 330 340 350
KSFLRNKLVK LLFPPVVVTN KRDLKKMFQR LQDLSLEYGE DVNEEDNDDE
360 370 380 390 400
AIHTKSRSYC RNKKAENSKK KSPKSNKKPK RKKQKFFTSW FTWGISITIG
410 420 430 440 450
ISFGCCVTYF VTAAYEHQTV KSLSLRPSIL ASLLSLDSSS DTINTPATAS
460 470 480 490 500
PSSTEQFLWF DKGTLQINFH SDGFIMKSLT IIKETWGKMN TFVLHALSKP
510 520
LKFLENLNKS SEFSIDESNR ILALGYILL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38059 Genomic DNA Translation: CAA86132.1 AY692933 Genomic DNA Translation: AAT92952.1 BK006942 Genomic DNA Translation: DAA08407.1 |
PIRi | S48388 |
RefSeqi | NP_012120.1, NM_001179494.1 |
Genome annotation databases
EnsemblFungii | YIL146C_mRNA; YIL146C; YIL146C |
GeneIDi | 854660 |
KEGGi | sce:YIL146C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38059 Genomic DNA Translation: CAA86132.1 AY692933 Genomic DNA Translation: AAT92952.1 BK006942 Genomic DNA Translation: DAA08407.1 |
PIRi | S48388 |
RefSeqi | NP_012120.1, NM_001179494.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VXW | X-ray | 3.00 | B | 85-90 | [»] | |
5WLP | NMR | - | A | 200-341 | [»] | |
SMRi | P40458 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34846, 242 interactors |
IntActi | P40458, 6 interactors |
MINTi | P40458 |
STRINGi | 4932.YIL146C |
PTM databases
iPTMneti | P40458 |
Proteomic databases
MaxQBi | P40458 |
PaxDbi | P40458 |
PRIDEi | P40458 |
Genome annotation databases
EnsemblFungii | YIL146C_mRNA; YIL146C; YIL146C |
GeneIDi | 854660 |
KEGGi | sce:YIL146C |
Organism-specific databases
SGDi | S000001408, ATG32 |
VEuPathDBi | FungiDB:YIL146C |
Phylogenomic databases
eggNOGi | ENOG502QY5V, Eukaryota |
HOGENOMi | CLU_039418_0_0_1 |
InParanoidi | P40458 |
OMAi | IPICQPG |
Miscellaneous databases
PROi | PR:P40458 |
RNActi | P40458, protein |
Family and domain databases
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG32_YEAST | |
Accessioni | P40458Primary (citable) accession number: P40458 Secondary accession number(s): D6VVE1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families