UniProtKB - P40433 (6P21_YEAST)
Protein
6-phosphofructo-2-kinase 1
Gene
PFK26
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Synthesis of fructose 2,6-bisphosphate.
Miscellaneous
Present with 1710 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.7.1.105
Activity regulationi
Phosphorylation results in the activation of the kinase activity.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 277 | Sequence analysis | 1 | |
Active sitei | 309 | Sequence analysis | 1 | |
Binding sitei | 343 | Fructose-6-phosphateBy similarity | 1 | |
Active sitei | 404 | Phosphoserine intermediateBy similarity | 1 | |
Active sitei | 497 | Sequence analysis | 1 | |
Active sitei | 565 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 190 – 197 | ATPSequence analysis | 8 |
GO - Molecular functioni
- 6-phosphofructo-2-kinase activity Source: SGD
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- fructose 2,6-bisphosphate metabolic process Source: SGD
- fructose metabolic process Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | YEAST:YIL107C-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phosphofructo-2-kinase 1 (EC:2.7.1.105)Short name: 6PF-2-K 1 Alternative name(s): Phosphofructokinase 2 I |
Gene namesi | Name:PFK26 Ordered Locus Names:YIL107C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001369, PFK26 |
VEuPathDBi | FungiDB:YIL107C |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000179976 | 1 – 827 | 6-phosphofructo-2-kinase 1Add BLAST | 827 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 92 | PhosphoserineCombined sources | 1 | |
Modified residuei | 157 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 644 | PhosphoserineCombined sources | 1 | |
Modified residuei | 652 | PhosphoserineCombined sources | 1 | |
Modified residuei | 659 | PhosphoserineCombined sources | 1 | |
Modified residuei | 667 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P40433 |
PaxDbi | P40433 |
PRIDEi | P40433 |
PTM databases
iPTMneti | P40433 |
Interactioni
Binary interactionsi
P40433
With | #Exp. | IntAct |
---|---|---|
SSB1 [P11484] | 2 | EBI-1956,EBI-8627 |
Protein-protein interaction databases
BioGRIDi | 34884, 185 interactors |
IntActi | P40433, 271 interactors |
MINTi | P40433 |
STRINGi | 4932.YIL107C |
Miscellaneous databases
RNActi | P40433, protein |
Family & Domainsi
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
HOGENOMi | CLU_006383_3_2_1 |
InParanoidi | P40433 |
OMAi | PIKQMRM |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
i Sequence
Sequence statusi: Complete.
P40433-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKPVDFSET SPVPPDIDLA PTQSPHHVAP SQDSSYDLLS RSSDDKIDAE
60 70 80 90 100
KGPHDELSKH LPLFQKRPLS DTPISSNWNS PGITEENTPS DSPENSATNL
110 120 130 140 150
KSLHRLHIND ETQLKNAKIP TNDTTDYMPP SDGANEVTRI DLKDIKSPTR
160 170 180 190 200
HHKRRPTTID VPGLTKSKTS PDGLISKEDS GSKLVIVMVG LPATGKSFIT
210 220 230 240 250
NKLSRFLNYS LYYCKVFNVG NTRRKFAKEH GLKDQDSKFF EPKNADSTRL
260 270 280 290 300
RDKWAMDTLD ELLDYLLEGS GSVGIFDATN TSRERRKNVL ARIRKRSPHL
310 320 330 340 350
KVLFLESVCS DHALVQKNIR LKLFGPDYKG KDPESSLKDF KSRLANYLKA
360 370 380 390 400
YEPIEDDENL QYIKMIDVGK KVIAYNIQGF LASQTVYYLL NFNLADRQIW
410 420 430 440 450
ITRSGESEDN VSGRIGGNSH LTPRGLRFAK SLPKFIARQR EIFYQNLMQQ
460 470 480 490 500
KKNNENTDGN IYNDFFVWTS MRARTIGTAQ YFNEDDYPIK QMKMLDELSA
510 520 530 540 550
GDYDGMTYPE IKNNFPEEFE KRQKDKLRYR YPGIGGESYM DVINRLRPVI
560 570 580 590 600
TELERIEDNV LIITHRVVAR ALLGYFMNLS MGIIANLDVP LHCVYCLEPK
610 620 630 640 650
PYGITWSLWE YDEASDSFSK VPQTDLNTTR VKEVGLVYNE RRYSVIPTAP
660 670 680 690 700
PSARSSFASD FLSRKRSNPT SASSSQSELS EQPKNSVSAQ TGSNNTTLIG
710 720 730 740 750
SNFNIKNENG DSRIPLSAPL MATNTSNNIL DGGGTSISIH RPRVVPNQNN
760 770 780 790 800
VNPLLANNNK AASNVPNVKK SAATPRQIFE IDKVDEKLSM LKNKSFLLHG
810 820
KDYPNNADNN DNEDIRAKTM NRSQSHV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 382 | A → R in AAA34858 (PubMed:1657152).Curated | 1 | |
Sequence conflicti | 477 | G → E AA sequence (PubMed:1851090).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38125 Genomic DNA Translation: CAA86273.1 M80801 Genomic DNA Translation: AAA34858.1 AY692819 Genomic DNA Translation: AAT92838.1 BK006942 Genomic DNA Translation: DAA08446.1 |
PIRi | S48465 |
RefSeqi | NP_012159.1, NM_001179455.1 |
Genome annotation databases
EnsemblFungii | YIL107C_mRNA; YIL107C; YIL107C |
GeneIDi | 854699 |
KEGGi | sce:YIL107C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z38125 Genomic DNA Translation: CAA86273.1 M80801 Genomic DNA Translation: AAA34858.1 AY692819 Genomic DNA Translation: AAT92838.1 BK006942 Genomic DNA Translation: DAA08446.1 |
PIRi | S48465 |
RefSeqi | NP_012159.1, NM_001179455.1 |
3D structure databases
SMRi | P40433 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34884, 185 interactors |
IntActi | P40433, 271 interactors |
MINTi | P40433 |
STRINGi | 4932.YIL107C |
PTM databases
iPTMneti | P40433 |
Proteomic databases
MaxQBi | P40433 |
PaxDbi | P40433 |
PRIDEi | P40433 |
Genome annotation databases
EnsemblFungii | YIL107C_mRNA; YIL107C; YIL107C |
GeneIDi | 854699 |
KEGGi | sce:YIL107C |
Organism-specific databases
SGDi | S000001369, PFK26 |
VEuPathDBi | FungiDB:YIL107C |
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
HOGENOMi | CLU_006383_3_2_1 |
InParanoidi | P40433 |
OMAi | PIKQMRM |
Enzyme and pathway databases
BioCyci | YEAST:YIL107C-MONOMER |
Miscellaneous databases
PROi | PR:P40433 |
RNActi | P40433, protein |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 6P21_YEAST | |
Accessioni | P40433Primary (citable) accession number: P40433 Secondary accession number(s): D6VVI0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IX
Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names