UniProtKB - P40429 (RL13A_HUMAN)
Protein
60S ribosomal protein L13a
Gene
RPL13A
Organism
Homo sapiens (Human)
Status
Functioni
Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.4 Publications
GO - Molecular functioni
- mRNA binding Source: GO_Central
- RNA binding Source: UniProtKB
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- cellular response to interferon-gamma Source: UniProtKB
- negative regulation of formation of translation preinitiation complex Source: UniProtKB
- negative regulation of translation Source: UniProtKB
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
- SRP-dependent cotranslational protein targeting to membrane Source: Reactome
- translation Source: UniProtKB
- translational initiation Source: Reactome
- viral transcription Source: Reactome
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein |
Biological process | Translation regulation |
Enzyme and pathway databases
PathwayCommonsi | P40429 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-156902, Peptide chain elongation R-HSA-1799339, SRP-dependent cotranslational protein targeting to membrane R-HSA-192823, Viral mRNA Translation R-HSA-2408557, Selenocysteine synthesis R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72764, Eukaryotic Translation Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9633012, Response of EIF2AK4 (GCN2) to amino acid deficiency R-HSA-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
SIGNORi | P40429 |
Protein family/group databases
MoonProti | P40429 |
Names & Taxonomyi
Protein namesi | Recommended name: 60S ribosomal protein L13aAlternative name(s): 23 kDa highly basic protein Large ribosomal subunit protein uL131 Publication |
Gene namesi | Name:RPL13A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000142541.16 |
HGNCi | HGNC:10304, RPL13A |
neXtProti | NX_P40429 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: Reactome
- cytosolic large ribosomal subunit Source: UniProtKB
Nucleus
Other locations
- cytoplasm Source: UniProtKB
- focal adhesion Source: UniProtKB
- GAIT complex Source: UniProtKB
- large ribosomal subunit Source: ProtInc
- membrane Source: UniProtKB
- ribonucleoprotein complex Source: UniProtKB
- ribosome Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 77 | S → A: Loss of interferon-gamma induced phosphorylation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 23521 |
OpenTargetsi | ENSG00000142541 |
PharmGKBi | PA38122 |
Miscellaneous databases
Pharosi | P40429, Tbio |
Chemistry databases
DrugBanki | DB02494, (S)-3-phenyllactic acid DB07374, Anisomycin DB08437, Puromycin |
Polymorphism and mutation databases
BioMutai | RPL13A |
DMDMi | 730451 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources3 Publications | |||
ChainiPRO_0000133769 | 2 – 203 | 60S ribosomal protein L13aAdd BLAST | 202 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources3 Publications | 1 | |
Modified residuei | 59 | CitrullineBy similarity | 1 | |
Modified residuei | 77 | Phosphoserine; by ZIPK/DAPK31 Publication | 1 | |
Modified residuei | 140 | CitrullineBy similarity | 1 | |
Modified residuei | 191 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Phosphorylation at Ser-77 upon interferon-gamma treatment in monocytes involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.2 Publications
Citrullinated by PADI4.By similarity
Keywords - PTMi
Acetylation, Citrullination, PhosphoproteinProteomic databases
EPDi | P40429 |
jPOSTi | P40429 |
MassIVEi | P40429 |
MaxQBi | P40429 |
PaxDbi | P40429 |
PeptideAtlasi | P40429 |
PRIDEi | P40429 |
ProteomicsDBi | 55374 |
TopDownProteomicsi | P40429 |
PTM databases
iPTMneti | P40429 |
MetOSitei | P40429 |
PhosphoSitePlusi | P40429 |
SwissPalmi | P40429 |
Expressioni
Gene expression databases
Bgeei | ENSG00000142541, Expressed in left ovary and 112 other tissues |
ExpressionAtlasi | P40429, baseline and differential |
Genevisiblei | P40429, HS |
Organism-specific databases
HPAi | ENSG00000142541, Low tissue specificity |
Interactioni
Subunit structurei
Component of the 60S ribosome.
Component of the GAIT complex.
Interacts with EIF4G1.
1 Publication3 PublicationsProtein-protein interaction databases
BioGRIDi | 117068, 187 interactors |
ComplexPortali | CPX-5183, 60S cytosolic large ribosomal subunit |
CORUMi | P40429 |
IntActi | P40429, 79 interactors |
MINTi | P40429 |
STRINGi | 9606.ENSP00000375730 |
Miscellaneous databases
RNActi | P40429, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the universal ribosomal protein uL13 family.Curated
Phylogenomic databases
eggNOGi | KOG3204, Eukaryota |
GeneTreei | ENSGT00390000010799 |
InParanoidi | P40429 |
OrthoDBi | 1312756at2759 |
PhylomeDBi | P40429 |
TreeFami | TF300159 |
Family and domain databases
CDDi | cd00392, Ribosomal_L13, 1 hit |
Gene3Di | 3.90.1180.10, 1 hit |
HAMAPi | MF_01366, Ribosomal_L13, 1 hit |
InterProi | View protein in InterPro IPR005822, Ribosomal_L13 IPR023563, Ribosomal_L13_CS IPR005755, Ribosomal_L13_euk/arc IPR036899, Ribosomal_L13_sf |
PANTHERi | PTHR11545, PTHR11545, 1 hit |
Pfami | View protein in Pfam PF00572, Ribosomal_L13, 1 hit |
SUPFAMi | SSF52161, SSF52161, 1 hit |
TIGRFAMsi | TIGR01077, L13_A_E, 1 hit |
PROSITEi | View protein in PROSITE PS00783, RIBOSOMAL_L13, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P40429-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN
60 70 80 90 100
KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLPH KTKRGQAALD
110 120 130 140 150
RLKVFDGIPP PYDKKKRMVV PAALKVVRLK PTRKFAYLGR LAHEVGWKYQ
160 170 180 190 200
AVTATLEEKR KEKAKIHYRK KKQLMRLRKQ AEKNVEKKID KYTEVLKTHG
LLV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8J015 | Q8J015_HUMAN | 60S ribosomal protein L13a | RPL13A RPL13a, hCG_1998057 | 142 | Annotation score: | ||
M0QYS1 | M0QYS1_HUMAN | 60S ribosomal protein L13a | RPL13A | 210 | Annotation score: | ||
M0QZU1 | M0QZU1_HUMAN | 60S ribosomal protein L13a | RPL13A | 46 | Annotation score: | ||
A0A096LPE0 | A0A096LPE0_HUMAN | 60S ribosomal protein L13a | RPL13A | 48 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56932 mRNA Translation: CAA40254.1 AB028893 Genomic DNA Translation: BAA88214.1 AK291120 mRNA Translation: BAF83809.1 CH471177 Genomic DNA Translation: EAW52495.1 BC000514 mRNA Translation: AAH00514.1 BC001675 mRNA Translation: AAH01675.1 BC001836 mRNA Translation: AAH01836.1 BC062537 mRNA Translation: AAH62537.1 BC065236 mRNA Translation: AAH65236.1 BC070223 mRNA Translation: AAH70223.1 BC071929 mRNA Translation: AAH71929.1 |
CCDSi | CCDS12768.1 |
PIRi | S29539 |
RefSeqi | NP_001257420.1, NM_001270491.1 NP_036555.1, NM_012423.3 |
Genome annotation databases
Ensembli | ENST00000391857; ENSP00000375730; ENSG00000142541 |
GeneIDi | 23521 |
KEGGi | hsa:23521 |
UCSCi | uc002pny.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56932 mRNA Translation: CAA40254.1 AB028893 Genomic DNA Translation: BAA88214.1 AK291120 mRNA Translation: BAF83809.1 CH471177 Genomic DNA Translation: EAW52495.1 BC000514 mRNA Translation: AAH00514.1 BC001675 mRNA Translation: AAH01675.1 BC001836 mRNA Translation: AAH01836.1 BC062537 mRNA Translation: AAH62537.1 BC065236 mRNA Translation: AAH65236.1 BC070223 mRNA Translation: AAH70223.1 BC071929 mRNA Translation: AAH71929.1 |
CCDSi | CCDS12768.1 |
PIRi | S29539 |
RefSeqi | NP_001257420.1, NM_001270491.1 NP_036555.1, NM_012423.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4UG0 | electron microscopy | - | LO | 1-203 | [»] | |
4V6X | electron microscopy | 5.00 | CO | 1-203 | [»] | |
5AJ0 | electron microscopy | 3.50 | AO | 1-203 | [»] | |
5LKS | electron microscopy | 3.60 | LO | 1-203 | [»] | |
5T2C | electron microscopy | 3.60 | I | 1-203 | [»] | |
6EK0 | electron microscopy | 2.90 | LO | 1-203 | [»] | |
6IP5 | electron microscopy | 3.90 | 2I | 1-203 | [»] | |
6IP6 | electron microscopy | 4.50 | 2I | 1-203 | [»] | |
6IP8 | electron microscopy | 3.90 | 2I | 1-203 | [»] | |
6LQM | electron microscopy | 3.09 | V | 1-203 | [»] | |
6LSR | electron microscopy | 3.13 | V | 1-203 | [»] | |
6LSS | electron microscopy | 3.23 | V | 1-203 | [»] | |
6LU8 | electron microscopy | 3.13 | V | 1-203 | [»] | |
6OLE | electron microscopy | 3.10 | P | 5-199 | [»] | |
6OLF | electron microscopy | 3.90 | P | 5-199 | [»] | |
6OLG | electron microscopy | 3.40 | AO | 5-199 | [»] | |
6OLI | electron microscopy | 3.50 | P | 5-199 | [»] | |
6OLZ | electron microscopy | 3.90 | AO | 5-199 | [»] | |
6OM0 | electron microscopy | 3.10 | P | 5-199 | [»] | |
6OM7 | electron microscopy | 3.70 | P | 5-199 | [»] | |
6QZP | electron microscopy | 2.90 | LO | 3-203 | [»] | |
6W6L | electron microscopy | 3.84 | P | 1-203 | [»] | |
6Y0G | electron microscopy | 3.20 | LO | 1-203 | [»] | |
6Y2L | electron microscopy | 3.00 | LO | 1-203 | [»] | |
6Y57 | electron microscopy | 3.50 | LO | 1-203 | [»] | |
6Y6X | electron microscopy | 2.80 | LO | 3-203 | [»] | |
6Z6L | electron microscopy | 3.00 | LO | 1-203 | [»] | |
6Z6M | electron microscopy | 3.10 | LO | 1-203 | [»] | |
6Z6N | electron microscopy | 2.90 | LO | 1-203 | [»] | |
6ZM7 | electron microscopy | 2.70 | LO | 1-203 | [»] | |
6ZME | electron microscopy | 3.00 | LO | 1-203 | [»] | |
6ZMI | electron microscopy | 2.60 | LO | 1-203 | [»] | |
6ZMO | electron microscopy | 3.10 | LO | 1-203 | [»] | |
SMRi | P40429 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117068, 187 interactors |
ComplexPortali | CPX-5183, 60S cytosolic large ribosomal subunit |
CORUMi | P40429 |
IntActi | P40429, 79 interactors |
MINTi | P40429 |
STRINGi | 9606.ENSP00000375730 |
Chemistry databases
DrugBanki | DB02494, (S)-3-phenyllactic acid DB07374, Anisomycin DB08437, Puromycin |
Protein family/group databases
MoonProti | P40429 |
PTM databases
iPTMneti | P40429 |
MetOSitei | P40429 |
PhosphoSitePlusi | P40429 |
SwissPalmi | P40429 |
Polymorphism and mutation databases
BioMutai | RPL13A |
DMDMi | 730451 |
Proteomic databases
EPDi | P40429 |
jPOSTi | P40429 |
MassIVEi | P40429 |
MaxQBi | P40429 |
PaxDbi | P40429 |
PeptideAtlasi | P40429 |
PRIDEi | P40429 |
ProteomicsDBi | 55374 |
TopDownProteomicsi | P40429 |
Protocols and materials databases
Antibodypediai | 45892, 98 antibodies |
DNASUi | 23521 |
Genome annotation databases
Ensembli | ENST00000391857; ENSP00000375730; ENSG00000142541 |
GeneIDi | 23521 |
KEGGi | hsa:23521 |
UCSCi | uc002pny.5, human |
Organism-specific databases
CTDi | 23521 |
DisGeNETi | 23521 |
EuPathDBi | HostDB:ENSG00000142541.16 |
GeneCardsi | RPL13A |
HGNCi | HGNC:10304, RPL13A |
HPAi | ENSG00000142541, Low tissue specificity |
neXtProti | NX_P40429 |
OpenTargetsi | ENSG00000142541 |
PharmGKBi | PA38122 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3204, Eukaryota |
GeneTreei | ENSGT00390000010799 |
InParanoidi | P40429 |
OrthoDBi | 1312756at2759 |
PhylomeDBi | P40429 |
TreeFami | TF300159 |
Enzyme and pathway databases
PathwayCommonsi | P40429 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-156902, Peptide chain elongation R-HSA-1799339, SRP-dependent cotranslational protein targeting to membrane R-HSA-192823, Viral mRNA Translation R-HSA-2408557, Selenocysteine synthesis R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72764, Eukaryotic Translation Termination R-HSA-9010553, Regulation of expression of SLITs and ROBOs R-HSA-9633012, Response of EIF2AK4 (GCN2) to amino acid deficiency R-HSA-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-HSA-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
SIGNORi | P40429 |
Miscellaneous databases
BioGRID-ORCSi | 23521, 514 hits in 855 CRISPR screens |
ChiTaRSi | RPL13A, human |
GeneWikii | RPL13A |
GenomeRNAii | 23521 |
Pharosi | P40429, Tbio |
PROi | PR:P40429 |
RNActi | P40429, protein |
Gene expression databases
Bgeei | ENSG00000142541, Expressed in left ovary and 112 other tissues |
ExpressionAtlasi | P40429, baseline and differential |
Genevisiblei | P40429, HS |
Family and domain databases
CDDi | cd00392, Ribosomal_L13, 1 hit |
Gene3Di | 3.90.1180.10, 1 hit |
HAMAPi | MF_01366, Ribosomal_L13, 1 hit |
InterProi | View protein in InterPro IPR005822, Ribosomal_L13 IPR023563, Ribosomal_L13_CS IPR005755, Ribosomal_L13_euk/arc IPR036899, Ribosomal_L13_sf |
PANTHERi | PTHR11545, PTHR11545, 1 hit |
Pfami | View protein in Pfam PF00572, Ribosomal_L13, 1 hit |
SUPFAMi | SSF52161, SSF52161, 1 hit |
TIGRFAMsi | TIGR01077, L13_A_E, 1 hit |
PROSITEi | View protein in PROSITE PS00783, RIBOSOMAL_L13, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RL13A_HUMAN | |
Accessioni | P40429Primary (citable) accession number: P40429 Secondary accession number(s): A8K505 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Ribosomal proteins
Ribosomal proteins families and list of entries