UniProtKB - P40417 (ERKA_DROME)
Mitogen-activated protein kinase ERK-A
rl
Functioni
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway to regulate proliferation, differentiation and effect cell fate decisions in various tissues (PubMed:8157002, PubMed:19965758, PubMed:22140578, PubMed:27552662).
Required downstream of phl/Raf in the sev/sevenless, tor/torso, and EGF receptor homolog Egfr signal transduction pathways (PubMed:8157002).
Required for embryonic epithelial tissue repair (PubMed:22140578).
During larval development, mediates Ptth/tor signaling leading to the production of ecdysone, a hormone required for the initiation of metamorphosis (PubMed:19965758).
4 PublicationsCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 67 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 162 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 44 – 52 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: FlyBase
- protein kinase binding Source: FlyBase
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: FlyBase
- protein serine kinase activity Source: RHEA
- transcription factor binding Source: FlyBase
GO - Biological processi
- behavioral response to ethanol Source: FlyBase
- branching involved in open tracheal system development Source: FlyBase
- cell surface receptor signaling pathway Source: GO_Central
- cellular response to arsenic-containing substance Source: FlyBase
- cellular response to cadmium ion Source: FlyBase
- cellular response to reactive oxygen species Source: FlyBase
- cellular response to starvation Source: FlyBase
- defense response to virus Source: FlyBase
- determination of adult lifespan Source: FlyBase
- dorsal appendage formation Source: FlyBase
- epidermal growth factor receptor signaling pathway Source: FlyBase
- ERK1 and ERK2 cascade Source: FlyBase
- fibroblast growth factor receptor signaling pathway Source: FlyBase
- imaginal disc-derived wing morphogenesis Source: FlyBase
- imaginal disc-derived wing vein specification Source: FlyBase
- insulin receptor signaling pathway Source: FlyBase
- intracellular signal transduction Source: GO_Central
- leg disc proximal/distal pattern formation Source: FlyBase
- lymph gland crystal cell differentiation Source: FlyBase
- lymph gland plasmatocyte differentiation Source: FlyBase
- MAPK cascade Source: UniProtKB
- metamorphosis Source: FlyBase
- mitotic cell cycle Source: FlyBase
- modulation of chemical synaptic transmission Source: FlyBase
- negative regulation of macroautophagy Source: FlyBase
- positive regulation of cell population proliferation Source: FlyBase
- positive regulation of cell size Source: FlyBase
- positive regulation of DNA-binding transcription factor activity Source: FlyBase
- positive regulation of photoreceptor cell differentiation Source: FlyBase
- positive regulation of wound healing Source: FlyBase
- R7 cell fate commitment Source: FlyBase
- regulation of heart morphogenesis Source: FlyBase
- regulation of response to drug Source: FlyBase
- sevenless signaling pathway Source: FlyBase
- terminal branching, open tracheal system Source: FlyBase
- terminal region determination Source: FlyBase
- torso signaling pathway Source: FlyBase
- tracheal pit formation in open tracheal system Source: FlyBase
- vascular endothelial growth factor receptor signaling pathway Source: FlyBase
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 1994 |
Reactomei | R-DME-110056, MAPK3 (ERK1) activation R-DME-112409, RAF-independent MAPK1/3 activation R-DME-112411, MAPK1 (ERK2) activation R-DME-1181150, Signaling by NODAL R-DME-1295596, Spry regulation of FGF signaling R-DME-1502540, Signaling by Activin R-DME-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-DME-170968, Frs2-mediated activation R-DME-198753, ERK/MAPK targets R-DME-202670, ERKs are inactivated R-DME-2029482, Regulation of actin dynamics for phagocytic cup formation R-DME-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription R-DME-2559580, Oxidative Stress Induced Senescence R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-2559585, Oncogene Induced Senescence R-DME-2871796, FCERI mediated MAPK activation R-DME-3371453, Regulation of HSF1-mediated heat shock response R-DME-375165, NCAM signaling for neurite out-growth R-DME-437239, Recycling pathway of L1 R-DME-445144, Signal transduction by L1 R-DME-450341, Activation of the AP-1 family of transcription factors R-DME-456926, Thrombin signalling through proteinase activated receptors (PARs) R-DME-5654726, Negative regulation of FGFR1 signaling R-DME-5654733, Negative regulation of FGFR4 signaling R-DME-5663213, RHO GTPases Activate WASPs and WAVEs R-DME-5673001, RAF/MAP kinase cascade R-DME-5674135, MAP2K and MAPK activation R-DME-5674499, Negative feedback regulation of MAPK pathway R-DME-5675221, Negative regulation of MAPK pathway R-DME-6798695, Neutrophil degranulation R-DME-74749, Signal attenuation R-DME-9634635, Estrogen-stimulated signaling through PRKCZ R-DME-9634638, Estrogen-dependent nuclear events downstream of ESR-membrane signaling |
SignaLinki | P40417 |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase ERK-A (EC:2.7.11.24)Alternative name(s): Extracellular-regulated kinase A Protein rolled |
Gene namesi | ORF Names:CG12559Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003256, rl |
VEuPathDBi | VectorBase:FBgn0003256 |
Subcellular locationi
Cytosol
- cytosol Source: FlyBase
Nucleus
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: GO_Central
- neuromuscular junction Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186309 | 1 – 376 | Mitogen-activated protein kinase ERK-AAdd BLAST | 376 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 198 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 200 | Phosphotyrosine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P40417 |
PTM databases
iPTMneti | P40417 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | FBgn0003256, Expressed in embryo and 26 other tissues |
ExpressionAtlasi | P40417, baseline and differential |
Genevisiblei | P40417, DM |
Interactioni
Binary interactionsi
P40417
GO - Molecular functioni
- protein kinase binding Source: FlyBase
- transcription factor binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 78013, 85 interactors |
DIPi | DIP-17266N |
IntActi | P40417, 14 interactors |
MINTi | P40417 |
STRINGi | 7227.FBpp0112426 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 326 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 289 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 198 – 200 | TXY | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
GeneTreei | ENSGT00940000156771 |
InParanoidi | P40417 |
OMAi | QAISNDH |
PhylomeDBi | P40417 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008349, MAPK_ERK1/2 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01770, ERK1ERK2MAPK |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEEFNSSGSV VNGTGSTEVP QSNAEVIRGQ IFEVGPRYIK LAYIGEGAYG
60 70 80 90 100
MVVSADDTLT NQRVAIKKIS PFEHQTYCQR TLREITILTR FKHENIIDIR
110 120 130 140 150
DILRVDSIDQ MRDVYIVQCL METDLYKLLK TQRLSNDHIC YFLYQILRGL
160 170 180 190 200
KYIHSANVLH RDLKPSNLLL NKTCDLKICD FGLARIADPE HDHTGFLTEY
210 220 230 240 250
VATRWYRAPE IMLNSKGYTK SIDIWSVGCI LAEMLSNRPI FPGKHYLDQL
260 270 280 290 300
NHILGVLGSP SRDDLECIIN EKARNYLESL PFKPNVPWAK LFPNADALAL
310 320 330 340 350
DLLGKMLTFN PHKRIPVEEA LAHPYLEQYY DPGDEPVAEV PFRINMENDD
360 370
ISRDALKSLI FEETLKFKER QPDNAP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8Y4W5 | A8Y4W5_DROME | RE73195p | rl 12559, 2, BcDNA:RE08694, CG18732, CT34260 | 190 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047792 | 1 – 110 | Missing in isoform H. CuratedAdd BLAST | 110 | |
Alternative sequenceiVSP_047793 | 1 – 45 | Missing in isoform G. CuratedAdd BLAST | 45 | |
Alternative sequenceiVSP_047794 | 46 – 53 | EGAYGMVV → MESALVIR in isoform G. Curated | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95124 mRNA Translation: AAA28677.1 AE013599 Genomic DNA Translation: EAA46310.2 AE013599 Genomic DNA Translation: EAA46311.2 AE013599 Genomic DNA Translation: EAA46312.4 AE013599 Genomic DNA Translation: EDP28106.2 AE013599 Genomic DNA Translation: EDP28107.1 AE013599 Genomic DNA Translation: EYR77323.1 AY070996 mRNA Translation: AAL48618.1 |
PIRi | A46036 B46036 |
RefSeqi | NP_001015121.3, NM_001015121.3 [P40417-2] NP_001015122.1, NM_001015122.3 [P40417-3] NP_001015123.1, NM_001015123.3 [P40417-3] NP_001104348.2, NM_001110878.4 [P40417-1] NP_001104349.1, NM_001110879.2 [P40417-3] NP_001287635.1, NM_001300706.2 [P40417-3] |
Genome annotation databases
EnsemblMetazoai | FBtr0113700; FBpp0112423; FBgn0003256 [P40417-3] FBtr0113702; FBpp0112425; FBgn0003256 [P40417-3] FBtr0113703; FBpp0112426; FBgn0003256 [P40417-3] FBtr0329919; FBpp0302952; FBgn0003256 [P40417-2] FBtr0329920; FBpp0302953; FBgn0003256 [P40417-1] FBtr0345337; FBpp0311493; FBgn0003256 [P40417-3] |
GeneIDi | 3354888 |
KEGGi | dme:Dmel_CG12559 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95124 mRNA Translation: AAA28677.1 AE013599 Genomic DNA Translation: EAA46310.2 AE013599 Genomic DNA Translation: EAA46311.2 AE013599 Genomic DNA Translation: EAA46312.4 AE013599 Genomic DNA Translation: EDP28106.2 AE013599 Genomic DNA Translation: EDP28107.1 AE013599 Genomic DNA Translation: EYR77323.1 AY070996 mRNA Translation: AAL48618.1 |
PIRi | A46036 B46036 |
RefSeqi | NP_001015121.3, NM_001015121.3 [P40417-2] NP_001015122.1, NM_001015122.3 [P40417-3] NP_001015123.1, NM_001015123.3 [P40417-3] NP_001104348.2, NM_001110878.4 [P40417-1] NP_001104349.1, NM_001110879.2 [P40417-3] NP_001287635.1, NM_001300706.2 [P40417-3] |
3D structure databases
SMRi | P40417 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 78013, 85 interactors |
DIPi | DIP-17266N |
IntActi | P40417, 14 interactors |
MINTi | P40417 |
STRINGi | 7227.FBpp0112426 |
PTM databases
iPTMneti | P40417 |
Proteomic databases
PaxDbi | P40417 |
Protocols and materials databases
DNASUi | 3354888 |
Genome annotation databases
EnsemblMetazoai | FBtr0113700; FBpp0112423; FBgn0003256 [P40417-3] FBtr0113702; FBpp0112425; FBgn0003256 [P40417-3] FBtr0113703; FBpp0112426; FBgn0003256 [P40417-3] FBtr0329919; FBpp0302952; FBgn0003256 [P40417-2] FBtr0329920; FBpp0302953; FBgn0003256 [P40417-1] FBtr0345337; FBpp0311493; FBgn0003256 [P40417-3] |
GeneIDi | 3354888 |
KEGGi | dme:Dmel_CG12559 |
Organism-specific databases
CTDi | 3354888 |
FlyBasei | FBgn0003256, rl |
VEuPathDBi | VectorBase:FBgn0003256 |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
GeneTreei | ENSGT00940000156771 |
InParanoidi | P40417 |
OMAi | QAISNDH |
PhylomeDBi | P40417 |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 1994 |
Reactomei | R-DME-110056, MAPK3 (ERK1) activation R-DME-112409, RAF-independent MAPK1/3 activation R-DME-112411, MAPK1 (ERK2) activation R-DME-1181150, Signaling by NODAL R-DME-1295596, Spry regulation of FGF signaling R-DME-1502540, Signaling by Activin R-DME-162658, Golgi Cisternae Pericentriolar Stack Reorganization R-DME-170968, Frs2-mediated activation R-DME-198753, ERK/MAPK targets R-DME-202670, ERKs are inactivated R-DME-2029482, Regulation of actin dynamics for phagocytic cup formation R-DME-2173796, SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription R-DME-2559580, Oxidative Stress Induced Senescence R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-2559585, Oncogene Induced Senescence R-DME-2871796, FCERI mediated MAPK activation R-DME-3371453, Regulation of HSF1-mediated heat shock response R-DME-375165, NCAM signaling for neurite out-growth R-DME-437239, Recycling pathway of L1 R-DME-445144, Signal transduction by L1 R-DME-450341, Activation of the AP-1 family of transcription factors R-DME-456926, Thrombin signalling through proteinase activated receptors (PARs) R-DME-5654726, Negative regulation of FGFR1 signaling R-DME-5654733, Negative regulation of FGFR4 signaling R-DME-5663213, RHO GTPases Activate WASPs and WAVEs R-DME-5673001, RAF/MAP kinase cascade R-DME-5674135, MAP2K and MAPK activation R-DME-5674499, Negative feedback regulation of MAPK pathway R-DME-5675221, Negative regulation of MAPK pathway R-DME-6798695, Neutrophil degranulation R-DME-74749, Signal attenuation R-DME-9634635, Estrogen-stimulated signaling through PRKCZ R-DME-9634638, Estrogen-dependent nuclear events downstream of ESR-membrane signaling |
SignaLinki | P40417 |
Miscellaneous databases
BioGRID-ORCSi | 3354888, 1 hit in 3 CRISPR screens |
ChiTaRSi | rl, fly |
GenomeRNAii | 3354888 |
PROi | PR:P40417 |
Gene expression databases
Bgeei | FBgn0003256, Expressed in embryo and 26 other tissues |
ExpressionAtlasi | P40417, baseline and differential |
Genevisiblei | P40417, DM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008349, MAPK_ERK1/2 IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01770, ERK1ERK2MAPK |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ERKA_DROME | |
Accessioni | P40417Primary (citable) accession number: P40417 Secondary accession number(s): A0A021WW06 Q9W5M3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | September 18, 2013 | |
Last modified: | February 23, 2022 | |
This is version 209 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families