UniProtKB - P40384 (SPO11_SCHPO)
Protein
Meiotic recombination protein rec12
Gene
rec12
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Required for formation of the double-strand breaks (DSBs) that initiate meiotic recombination (PubMed:10882124). Required for crossover recombination and chiasmatic segregation of chromosomes during meiosis I. Also involved in the faithful equational segregation of chromosomes during meiosis II (PubMed:12437782, PubMed:16303567).3 Publications
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 98 | Nucleophile1 Publication | 1 | |
Metal bindingi | 179 | MagnesiumBy similarity | 1 | |
Metal bindingi | 229 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: PomBase
- DNA binding Source: GO_Central
- DNA end binding Source: PomBase
- endodeoxyribonuclease activity, producing 3'-phosphomonoesters Source: PomBase
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- homologous chromosome orientation involved in meiotic metaphase I plate congression Source: PomBase
- meiotic DNA double-strand break formation Source: PomBase
- meiotic DNA double-strand break processing Source: GO_Central
- reciprocal meiotic recombination Source: PomBase
Keywordsi
Biological process | Chromosome partition, Meiosis |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Meiotic recombination protein rec121 PublicationAlternative name(s): SPO11 protein homolog1 Publication |
Gene namesi | Name:rec121 Publication ORF Names:SPAC17A5.11Imported |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC17A5.11, rec12 |
VEuPathDBi | FungiDB:SPAC17A5.11 |
Subcellular locationi
Nucleus
- nuclear chromosome Source: PomBase
- nucleus Source: PomBase
Other locations
- cytoplasm Source: UniProtKB-SubCell
- site of double-strand break Source: PomBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 98 | Y → F: Errors in meiosis II segregation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145477 | 1 – 345 | Meiotic recombination protein rec12Add BLAST | 345 |
Proteomic databases
PaxDbi | P40384 |
Expressioni
Developmental stagei
Most abundant at 2 hours after induction of meiosis, at the time of premeiotic DNA synthesis. Found at much lower levels before and after this time.1 Publication
Interactioni
Subunit structurei
Component of the DSB catalytic core (DSBC) complex, composed of at least rec12, rec6 and rec14. The complex interacts with mde2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 278626, 56 interactors |
STRINGi | 4896.SPAC17A5.11.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the TOP6A family.Curated
Phylogenomic databases
eggNOGi | KOG2795, Eukaryota |
HOGENOMi | CLU_037229_0_1_1 |
InParanoidi | P40384 |
OMAi | EIEWLDE |
PhylomeDBi | P40384 |
Family and domain databases
CDDi | cd00223, TOPRIM_TopoIIB_SPO, 1 hit |
InterProi | View protein in InterPro IPR002815, Spo11/TopoVI_A IPR013049, Spo11/TopoVI_A_N IPR036078, Spo11/TopoVI_A_sf IPR034136, TOPRIM_Topo6A/Spo11 |
PANTHERi | PTHR10848, PTHR10848, 1 hit |
Pfami | View protein in Pfam PF04406, TP6A_N, 1 hit |
PRINTSi | PR01550, TOP6AFAMILY |
SUPFAMi | SSF56726, SSF56726, 1 hit |
i Sequence
Sequence statusi: Complete.
P40384-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNSNDKKKVV RSWIEQFVHD FVEQLSKPTK DSVNVALKRR KHNSWNGSLD
60 70 80 90 100
SKANERQKVK VFSFPRNETT IAQLFRVLDC VHEAVISDTV ITKRDIYYRD
110 120 130 140 150
VDLFKRQTVV DELLGDISNT IGCSRSDLNV EASAKGLVFG SIHIALENGT
160 170 180 190 200
VITATKPLLI SHHRISSITS TAKWVLVIEK EAVFQTLTEE ALADTIIVTA
210 220 230 240 250
KGFPDLMTRK FLVKLAKALP DAKFFGIFDW DPHGLCIYSC FKYGSNAYSH
260 270 280 290 300
EPHSQLRNLQ LLGPLYEDIF NKNQEFSLKL NKRDIKMITT LLQFEGFQKE
310 320 330 340
PVVREQLQRM LFIQKKAEIQ AILEFPSWIK GKLADADKSG KHSVR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF195027 mRNA Translation: AAF06017.1 CU329670 Genomic DNA Translation: CAB11511.1 L14774 Genomic DNA Translation: AAA35332.1 |
PIRi | T37826 |
RefSeqi | NP_593479.1, NM_001018912.1 |
Genome annotation databases
EnsemblFungii | SPAC17A5.11.1; SPAC17A5.11.1:pep; SPAC17A5.11 |
GeneIDi | 2542150 |
KEGGi | spo:SPAC17A5.11 |
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF195027 mRNA Translation: AAF06017.1 CU329670 Genomic DNA Translation: CAB11511.1 L14774 Genomic DNA Translation: AAA35332.1 |
PIRi | T37826 |
RefSeqi | NP_593479.1, NM_001018912.1 |
3D structure databases
SMRi | P40384 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 278626, 56 interactors |
STRINGi | 4896.SPAC17A5.11.1 |
Proteomic databases
PaxDbi | P40384 |
Genome annotation databases
EnsemblFungii | SPAC17A5.11.1; SPAC17A5.11.1:pep; SPAC17A5.11 |
GeneIDi | 2542150 |
KEGGi | spo:SPAC17A5.11 |
Organism-specific databases
PomBasei | SPAC17A5.11, rec12 |
VEuPathDBi | FungiDB:SPAC17A5.11 |
Phylogenomic databases
eggNOGi | KOG2795, Eukaryota |
HOGENOMi | CLU_037229_0_1_1 |
InParanoidi | P40384 |
OMAi | EIEWLDE |
PhylomeDBi | P40384 |
Miscellaneous databases
PROi | PR:P40384 |
Family and domain databases
CDDi | cd00223, TOPRIM_TopoIIB_SPO, 1 hit |
InterProi | View protein in InterPro IPR002815, Spo11/TopoVI_A IPR013049, Spo11/TopoVI_A_N IPR036078, Spo11/TopoVI_A_sf IPR034136, TOPRIM_Topo6A/Spo11 |
PANTHERi | PTHR10848, PTHR10848, 1 hit |
Pfami | View protein in Pfam PF04406, TP6A_N, 1 hit |
PRINTSi | PR01550, TOP6AFAMILY |
SUPFAMi | SSF56726, SSF56726, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPO11_SCHPO | |
Accessioni | P40384Primary (citable) accession number: P40384 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families