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Entry version 144 (16 Oct 2019)
Sequence version 1 (01 Feb 1995)
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Protein

Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

ALG8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc2-PP-Dol.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: SGD
  • dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity Source: GO_Central

GO - Biological processi

  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein N-linked glycosylation Source: SGD

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MetaCyc:YOR067C-MONOMER
YEAST:YOR067C-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.4.1.265 984

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00378

Protein family/group databases

Carbohydrate-Active enZymes

More...
CAZyi
GT57 Glycosyltransferase Family 57

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ALG8
Ordered Locus Names:YOR067C
ORF Names:YOR29-18
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XV

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YOR067C

Saccharomyces Genome Database

More...
SGDi
S000005593 ALG8

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 42LumenalSequence analysisAdd BLAST42
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei43 – 63HelicalSequence analysisAdd BLAST21
Topological domaini64 – 92CytoplasmicSequence analysisAdd BLAST29
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 139LumenalSequence analysisAdd BLAST26
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 168CytoplasmicSequence analysis8
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 219LumenalSequence analysisAdd BLAST30
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 266CytoplasmicSequence analysisAdd BLAST26
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 307LumenalSequence analysisAdd BLAST20
Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
Topological domaini329 – 382CytoplasmicSequence analysisAdd BLAST54
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Topological domaini404 – 419LumenalSequence analysisAdd BLAST16
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Topological domaini441 – 447CytoplasmicSequence analysis7
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 470LumenalSequence analysis2
Transmembranei471 – 491HelicalSequence analysisAdd BLAST21
Topological domaini492 – 504CytoplasmicSequence analysisAdd BLAST13
Transmembranei505 – 525HelicalSequence analysisAdd BLAST21
Topological domaini526 – 543LumenalSequence analysisAdd BLAST18
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 577CytoplasmicSequence analysisAdd BLAST13

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001741681 – 577Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST577

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P40351

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P40351

PRoteomics IDEntifications database

More...
PRIDEi
P40351

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P40351

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
34466, 234 interactors

Database of interacting proteins

More...
DIPi
DIP-7542N

Molecular INTeraction database

More...
MINTi
P40351

STRING: functional protein association networks

More...
STRINGi
4932.YOR067C

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000168174

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P40351

KEGG Orthology (KO)

More...
KOi
K03849

Identification of Orthologs from Complete Genome Data

More...
OMAi
DFDVHRN

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR039487 ALG8
IPR004856 Glyco_trans_ALG6/ALG8

The PANTHER Classification System

More...
PANTHERi
PTHR12413 PTHR12413, 1 hit
PTHR12413:SF2 PTHR12413:SF2, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03155 Alg6_Alg8, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P40351-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKGDRSRQNM AVTKKAKLKK NDEPKKVLKT AATEKGEGSK RYSLWNFWIS
60 70 80 90 100
TLFLKLLLIP DYFSTDFDVH RNWLAITNKL PISEWYYEHT SQWTLDYPPF
110 120 130 140 150
FAYFEWFLSQ FVPKSVRDDG CLDIVEIGKF GLPTIVFQRL TVIFSEILLF
160 170 180 190 200
VILQIYINTT KLSERSQSFV VASSIVLSPG FLIIDHIHFQ YNGFLFAILI
210 220 230 240 250
GSIVAAKNKR YILCAVLYTT AICFKHIFLY LAPCYFVFLL RAYVLNVNNF
260 270 280 290 300
KFKSYKDFLF LIRWANLLKL ATVVVGIFTI CFLPFAHQMP QVLSRLFPFS
310 320 330 340 350
RGLTHAYWAP NFWALYSFMD KILTTVMLKL PYVHTFATKF IKPPLIPQNI
360 370 380 390 400
KEINERLAAN NNGSKGLVQD VFFVILPQIP PKLTFILTIF YQVLAVLPLL
410 420 430 440 450
FDPSFKRFVG SLTLCGLASF LFGWHVHEKA IMLVIIPFTF LVGFDRRLLV
460 470 480 490 500
PFMLVASAGY VSLYPLLYKG QDFFIKTLYT YVWCIIYFAA FRKTTKISSS
510 520 530 540 550
VERRIFFLDR LALTYIFSLL PIVTVLQILD EVKWRYSFLQ KFEFLGLMIY
560 570
SVYCSLGIIS SWFALSWLYN FDELLWQ
Length:577
Mass (Da):67,385
Last modified:February 1, 1995 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA59716F526B7B5A6
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X75929 Genomic DNA Translation: CAA53533.1
Z74975 Genomic DNA Translation: CAA99260.1
Z70678 Genomic DNA Translation: CAA94552.1
BK006948 Genomic DNA Translation: DAA10846.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S47961

NCBI Reference Sequences

More...
RefSeqi
NP_014710.1, NM_001183486.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YOR067C_mRNA; YOR067C; YOR067C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
854233

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YOR067C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75929 Genomic DNA Translation: CAA53533.1
Z74975 Genomic DNA Translation: CAA99260.1
Z70678 Genomic DNA Translation: CAA94552.1
BK006948 Genomic DNA Translation: DAA10846.1
PIRiS47961
RefSeqiNP_014710.1, NM_001183486.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGridi34466, 234 interactors
DIPiDIP-7542N
MINTiP40351
STRINGi4932.YOR067C

Protein family/group databases

CAZyiGT57 Glycosyltransferase Family 57

PTM databases

iPTMnetiP40351

Proteomic databases

MaxQBiP40351
PaxDbiP40351
PRIDEiP40351

Genome annotation databases

EnsemblFungiiYOR067C_mRNA; YOR067C; YOR067C
GeneIDi854233
KEGGisce:YOR067C

Organism-specific databases

EuPathDBiFungiDB:YOR067C
SGDiS000005593 ALG8

Phylogenomic databases

HOGENOMiHOG000168174
InParanoidiP40351
KOiK03849
OMAiDFDVHRN

Enzyme and pathway databases

UniPathwayiUPA00378
BioCyciMetaCyc:YOR067C-MONOMER
YEAST:YOR067C-MONOMER
BRENDAi2.4.1.265 984
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P40351

Family and domain databases

InterProiView protein in InterPro
IPR039487 ALG8
IPR004856 Glyco_trans_ALG6/ALG8
PANTHERiPTHR12413 PTHR12413, 1 hit
PTHR12413:SF2 PTHR12413:SF2, 1 hit
PfamiView protein in Pfam
PF03155 Alg6_Alg8, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiALG8_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P40351
Secondary accession number(s): D6W2D0
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 16, 2019
This is version 144 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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