RFC2

Functionⁱ

Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. RFC2 binds ATP and single-stranded DNA.3 Publications

Miscellaneous

Present with 4610 molecules/cell in log phase SD medium.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	28	ATP; via carbonyl oxygen	1
Binding siteⁱ	32	ATP	1
Binding siteⁱ	171	ATP	1
Binding siteⁱ	229	ATP	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	65 – 73	ATP		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
DNA binding Source: UniProtKB-KW
purine nucleotide binding
Source: SGD
Inferred from sequence or structural similarityⁱ
- 7651383

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Cell cycle, DNA replication
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-31701-MONOMER
Reactomeⁱ	R-SCE-110312 Translesion synthesis by REV1 R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex R-SCE-110320 Translesion Synthesis by POLH R-SCE-174411 Polymerase switching on the C-strand of the telomere R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair R-SCE-5655862 Translesion synthesis by POLK R-SCE-5656121 Translesion synthesis by POLI R-SCE-5656169 Termination of translesion DNA synthesis R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-SCE-5696400 Dual Incision in GG-NER R-SCE-6782135 Dual incision in TC-NER R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091 Polymerase switching

BioCycⁱ

YEAST:G3O-31701-MONOMER

Reactomeⁱ

R-SCE-110312 Translesion synthesis by REV1
R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-110320 Translesion Synthesis by POLH
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5655862 Translesion synthesis by POLK
R-SCE-5656121 Translesion synthesis by POLI
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-69091 Polymerase switching

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Replication factor C subunit 2 Short name: Replication factor C2 Alternative name(s): Activator 1 41 kDa subunit
Gene namesⁱ	Name:RFC2 Ordered Locus Names:YJR068W ORF Names:J1808
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome X

Organism-specific databases

EuPathDBⁱ	FungiDB:YJR068W
Saccharomyces Genome Database More...SGDⁱ	S000003829 RFC2

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000121760	1 – 353	Replication factor C subunit 2Add BLAST		353

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	1	N-acetylmethionineCombined sources		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P40348
PaxDbⁱ	P40348
PRIDEⁱ	P40348

PTM databases

iPTMnetⁱ	P40348

iPTMnetⁱ

P40348

Interactionⁱ

Subunit structureⁱ

Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Component of the RAD24-RFC complex which consists of RAD14, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex. Component of the ELG1-RFC complex which consists of ELG1, RFC2, RFC3, RFC4 and RFC5. Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. RFC2 interacts with ECO1.9 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CTF18	P49956	2	EBI-14992,EBI-4560
CTF8	P38877	2	EBI-14992,EBI-5216
ELG1	Q12050	2	EBI-14992,EBI-32195
RAD24	P32641	5	EBI-14992,EBI-14675
RFC3	P38629	4	EBI-14992,EBI-15000

Protein-protein interaction databases

BioGridⁱ	33825, 338 interactors
ComplexPortalⁱ	CPX-1731 CTF18-RFC complex CPX-1807 Rad17 RFC-like complex CPX-422 ELG1-RFC complex CPX-545 DNA replication factor C complex
Database of interacting proteins More...DIPⁱ	DIP-2528N
IntActⁱ	P40348, 53 interactors
Molecular INTeraction database More...MINTⁱ	P40348
STRINGⁱ	4932.YJR068W

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	27 – 30	Combined sources	4
Turnⁱ	36 – 38	Combined sources	3
Helixⁱ	46 – 52	Combined sources	7
Beta strandⁱ	61 – 64	Combined sources	4
Helixⁱ	71 – 88	Combined sources	18
Beta strandⁱ	91 – 95	Combined sources	5
Beta strandⁱ	97 – 99	Combined sources	3
Helixⁱ	103 – 106	Combined sources	4
Helixⁱ	109 – 115	Combined sources	7
Helixⁱ	125 – 128	Combined sources	4
Beta strandⁱ	135 – 139	Combined sources	5
Helixⁱ	142 – 144	Combined sources	3
Helixⁱ	147 – 159	Combined sources	13
Turnⁱ	160 – 163	Combined sources	4
Beta strandⁱ	164 – 171	Combined sources	8
Helixⁱ	173 – 175	Combined sources	3
Helixⁱ	178 – 183	Combined sources	6
Beta strandⁱ	184 – 188	Combined sources	5
Helixⁱ	194 – 206	Combined sources	13
Turnⁱ	207 – 209	Combined sources	3
Helixⁱ	214 – 223	Combined sources	10
Helixⁱ	228 – 237	Combined sources	10
Helixⁱ	239 – 245	Combined sources	7
Helixⁱ	253 – 260	Combined sources	8
Helixⁱ	266 – 276	Combined sources	11
Helixⁱ	280 – 292	Combined sources	13
Helixⁱ	300 – 310	Combined sources	11
Beta strandⁱ	312 – 314	Combined sources	3
Helixⁱ	316 – 333	Combined sources	18
Helixⁱ	339 – 352	Combined sources	14

Show more details

3D structure databases

ProteinModelPortalⁱ	P40348
SMRⁱ	P40348
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P40348

EvolutionaryTraceⁱ

P40348

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074917
HOGENOMⁱ	HOG000224154
InParanoidⁱ	P40348
KEGG Orthology (KO) More...KOⁱ	K10755
OMAⁱ	EADSMTH
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C39MN

Family and domain databases

InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR003959 ATPase_AAA_core IPR008921 DNA_pol3_clamp-load_cplx_C IPR027417 P-loop_NTPase IPR013748 Rep_factorC_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00004 AAA, 1 hit PF08542 Rep_fac_C, 1 hit
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit
SUPFAMⁱ	SSF48019 SSF48019, 1 hit SSF52540 SSF52540, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P40348-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFEGFGPNKK RKISKLAAEQ SLAQQPWVEK YRPKNLDEVT AQDHAVTVLK 
        60         70         80         90        100
KTLKSANLPH MLFYGPPGTG KTSTILALTK ELYGPDLMKS RILELNASDE 
       110        120        130        140        150
RGISIVREKV KNFARLTVSK PSKHDLENYP CPPYKIIILD EADSMTADAQ 
       160        170        180        190        200
SALRRTMETY SGVTRFCLIC NYVTRIIDPL ASRCSKFRFK ALDASNAIDR 
       210        220        230        240        250
LRFISEQENV KCDDGVLERI LDISAGDLRR GITLLQSASK GAQYLGDGKN 
       260        270        280        290        300
ITSTQVEELA GVVPHDILIE IVEKVKSGDF DEIKKYVNTF MKSGWSAASV 
       310        320        330        340        350
VNQLHEYYIT NDNFDTNFKN QISWLLFTTD SRLNNGTNEH IQLLNLLVKI 

SQL

Length:353

Mass (Da):39,742

Last modified:February 1, 1995 - v1

Checksum:ⁱ59B2C4223B0D13BF

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	12	K → R in AAS56246 (PubMed:17322287).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	D28499 Genomic DNA Translation: BAA05858.1 U26028 Genomic DNA Translation: AAC49061.1 Z49568 Genomic DNA Translation: CAA89596.1 L47993 Genomic DNA Translation: AAB39294.1 AY557920 Genomic DNA Translation: AAS56246.1 BK006943 Genomic DNA Translation: DAA08855.1
PIRⁱ	S45531
NCBI Reference Sequences More...RefSeqⁱ	NP_012602.3, NM_001181726.3

Genome annotation databases

EnsemblFungiⁱ	YJR068W; YJR068W; YJR068W
GeneIDⁱ	853531
KEGGⁱ	sce:YJR068W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P40348	Rfc2p		YEASC		353	UniRef90_P40348
RFC2-like protein		SACEU		353
Rfc2p		SACAR		353
Replication factor C subunit 2		YEAS1		353
K7_Rfc2p		YEASK		353
+13

Protein	Similar proteins		Species	Score	Length	Source
P40348	Rfc2p		YEASC		353	UniRef50_P40348
Rfc2p		SACAR		353
Rfc2p		YEASO		353
RFC2-like protein		SACK1		353
Replication factor C subunit 2		YEAS1		353
+68

Entry informationⁱ

Entry nameⁱ	RFC2_YEAST
Accessionⁱ	P40348Primary (citable) accession number: P40348 Secondary accession number(s): D6VWN9, E9P8U1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
	Last sequence update:	February 1, 1995
	Last modified:	September 12, 2018
	This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniParc

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UniRef

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Supporting data

UniProtKB - P40348 (RFC2_YEAST)

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Replication factor C subunit 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ