UniProtKB - P40343 (VPS27_YEAST)
Protein
Vacuolar protein sorting-associated protein 27
Gene
VPS27
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane (PubMed:3062374, PubMed:1493335, PubMed:9265642, PubMed:11416128, PubMed:12055639, PubMed:11872141, PubMed:12900393, PubMed:14581452, PubMed:15166140, PubMed:15107463, PubMed:15086794, PubMed:17135292, PubMed:18508771). Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi (PubMed:11208109, PubMed:8649377, PubMed:9015300). Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC (PubMed:8649377). Might also function as an alternate adapter in the COPIb clathrin-like coat (PubMed:17101773).17 Publications
Miscellaneous
Present with 172 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 170 – 230 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphatidylinositol-3-phosphate binding Source: SGD
- protein domain specific binding Source: CAFA
- protein heterodimerization activity Source: CAFA
- ubiquitin binding Source: SGD
GO - Biological processi
- ATP export Source: SGD
- late endosome to vacuole transport Source: SGD
- lysosomal microautophagy Source: SGD
- microlipophagy Source: SGD
- multivesicular body sorting pathway Source: CAFA
- positive regulation of protein maturation Source: CAFA
- protein retention in Golgi apparatus Source: SGD
- protein secretion Source: CAFA
- protein targeting to vacuole Source: SGD
- protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
Keywordsi
Ligand | Metal-binding, Zinc |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 27Alternative name(s): Golgi retention defective protein 11 |
Gene namesi | Name:VPS27 Synonyms:DID7, GRD11, SSV17, VPL23, VPT27 Ordered Locus Names:YNR006W ORF Names:N2038 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005289, VPS27 |
VEuPathDBi | FungiDB:YNR006W |
Subcellular locationi
Endosome
- Endosome membrane 6 Publications; Peripheral membrane protein 6 Publications; Cytoplasmic side 6 Publications
Endosome
- endosome Source: SGD
- endosome membrane Source: UniProtKB-SubCell
- ESCRT-0 complex Source: SGD
Vacuole
- vacuolar membrane Source: SGD
Other locations
- protein-containing complex Source: CAFA
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 185 – 186 | LL → AA: Decreases the association to PtdIns(3)P containing membranes. 1 Publication | 2 | |
Mutagenesisi | 193 | R → A: Decreases the association to PtdIns(3)P containing membranes. 1 Publication | 1 | |
Mutagenesisi | 270 | S → D: Reduces strongly the ubiquitin-binding activity. 1 Publication | 1 | |
Mutagenesisi | 313 | S → D: Reduces strongly the ubiquitin-binding activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065893 | 1 – 622 | Vacuolar protein sorting-associated protein 27Add BLAST | 622 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 157 | PhosphoserineCombined sources | 1 | |
Cross-linki | 294 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 495 | PhosphoserineCombined sources | 1 | |
Modified residuei | 613 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P40343 |
PaxDbi | P40343 |
PRIDEi | P40343 |
PTM databases
iPTMneti | P40343 |
Interactioni
Subunit structurei
Component of the ESCRT-0 complex composed of HSE1 and VPS27 (PubMed:12055639, PubMed:14581452, PubMed:15086794).
Interacts with ENT3 and ENT5, the ESCRT-I subunits VPS23 and VPS28 and with the COPIb subunits SEC27, SEC28 and SEC33 (PubMed:12900393, PubMed:15107463, PubMed:17101773). May form a complex composed of VPS27, HSE1 and DOA1 (PubMed:18508771).
Interacts with DOA1 (PubMed:18508771).
Interacts with ubiquitin (PubMed:11988742, PubMed:12750381, PubMed:14581452).
9 PublicationsBinary interactionsi
Hide detailsP40343
With | #Exp. | IntAct |
---|---|---|
HSE1 [P38753] | 8 | EBI-20380,EBI-1382 |
SEC28 [P40509] | 2 | EBI-20380,EBI-4884 |
STP22 [P25604] | 7 | EBI-20380,EBI-411625 |
UBB [P0CG53] from Bos taurus. | 6 | EBI-20380,EBI-5333021 |
GO - Molecular functioni
- protein domain specific binding Source: CAFA
- protein heterodimerization activity Source: CAFA
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 35831, 753 interactors |
ComplexPortali | CPX-1622, ESCRT-0 complex |
DIPi | DIP-1741N |
IntActi | P40343, 7 interactors |
MINTi | P40343 |
STRINGi | 4932.YNR006W |
Miscellaneous databases
RNActi | P40343, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40343 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40343 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 149 | VHSPROSITE-ProRule annotationAdd BLAST | 132 | |
Domaini | 258 – 277 | UIM 1PROSITE-ProRule annotationAdd BLAST | 20 | |
Domaini | 301 – 320 | UIM 2PROSITE-ProRule annotationAdd BLAST | 20 |
Domaini
The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns3P) which is required for the association to endosomal membranes.
Both IUM domains are necessary for efficient binding to ubiquitin.
Sequence similaritiesi
Belongs to the VPS27 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 170 – 230 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1818, Eukaryota |
GeneTreei | ENSGT00940000170673 |
HOGENOMi | CLU_011862_2_0_1 |
InParanoidi | P40343 |
OMAi | VCSREFM |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR008942, ENTH_VHS IPR017073, Ubi-bd_Hrs_VPS27 IPR003903, UIM_dom IPR002014, VHS_dom IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF02809, UIM, 2 hits PF00790, VHS, 1 hit |
PIRSFi | PIRSF036956, Hrs_Vps27, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00726, UIM, 2 hits SM00288, VHS, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50330, UIM, 2 hits PS50179, VHS, 1 hit PS50178, ZF_FYVE, 1 hit |
i Sequence
Sequence statusi: Complete.
P40343-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVSTPSELD ALIEQATSES IPNGDLDLPI ALEISDVLRS RRVNPKDSMR
60 70 80 90 100
CIKKRILNTA DNPNTQLSSW KLTNICVKNG GTPFIKEICS REFMDTMEHV
110 120 130 140 150
ILREDSNEEL SELVKTILYE LYVAFKNDSQ LNYVAKVYDK LISRGIKFPE
160 170 180 190 200
KLTLSNSPTA MFDSKTPADW IDSDACMICS KKFSLLNRKH HCRSCGGVFC
210 220 230 240 250
QEHSSNSIPL PDLGIYEPVR VCDSCFEDYD LKRHDDSKKS KKHRHKRKKD
260 270 280 290 300
RDYSTPEDEE ELIRKAIELS LKESRNSASS EPIVPVVESK NEVKRQEIEE
310 320 330 340 350
EEDPDLKAAI QESLREAEEA KLRSERQKAS RQMQPQQPSP QPQPIHSVDL
360 370 380 390 400
SDEEKDSIYM FASLVEKMKS RPLNEILEDS KLQNLAQRVF ASKARLNYAL
410 420 430 440 450
NDKAQKYNTL IEMNGKISEI MNIYDRLLEQ QLQSINLSQQ YTLPQVPSDP
460 470 480 490 500
YNYLTENVQN PAESYQTPPL QQLSSHQYKP QQDVSRQQSV KANSSPTTNI
510 520 530 540 550
DHLKTIDVTP HAQQKPQSHV ELAPSDPPYP KEEAEDEGTQ AVQDEESSTQ
560 570 580 590 600
ESRERPYPVE TENGETSINK RPQGITRYDF PTVPARKFVQ PESTVPLPAS
610 620
SSEIPIKEER PPSPQEELLI EL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 321 – 322 | KL → NV in AAA96002 (PubMed:7593183).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U24218 Genomic DNA Translation: AAA96002.1 X77395 Genomic DNA Translation: CAA54574.1 Z71620 Genomic DNA Translation: CAA96282.1 BK006947 Genomic DNA Translation: DAA10547.1 |
PIRi | S45129 |
RefSeqi | NP_014403.3, NM_001183183.3 |
Genome annotation databases
EnsemblFungii | YNR006W_mRNA; YNR006W; YNR006W |
GeneIDi | 855739 |
KEGGi | sce:YNR006W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U24218 Genomic DNA Translation: AAA96002.1 X77395 Genomic DNA Translation: CAA54574.1 Z71620 Genomic DNA Translation: CAA96282.1 BK006947 Genomic DNA Translation: DAA10547.1 |
PIRi | S45129 |
RefSeqi | NP_014403.3, NM_001183183.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1O06 | X-ray | 1.45 | A | 301-320 | [»] | |
1Q0V | NMR | - | A | 249-329 | [»] | |
1Q0W | NMR | - | A | 255-278 | [»] | |
1VFY | X-ray | 1.15 | A | 163-230 | [»] | |
2KDI | NMR | - | A | 258-277 | [»] | |
2PJW | X-ray | 3.01 | V | 348-438 | [»] | |
3R42 | X-ray | 1.87 | B | 445-453 | [»] | |
6NJG | X-ray | 2.35 | B | 256-278 | [»] | |
SMRi | P40343 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35831, 753 interactors |
ComplexPortali | CPX-1622, ESCRT-0 complex |
DIPi | DIP-1741N |
IntActi | P40343, 7 interactors |
MINTi | P40343 |
STRINGi | 4932.YNR006W |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family |
PTM databases
iPTMneti | P40343 |
Proteomic databases
MaxQBi | P40343 |
PaxDbi | P40343 |
PRIDEi | P40343 |
Genome annotation databases
EnsemblFungii | YNR006W_mRNA; YNR006W; YNR006W |
GeneIDi | 855739 |
KEGGi | sce:YNR006W |
Organism-specific databases
SGDi | S000005289, VPS27 |
VEuPathDBi | FungiDB:YNR006W |
Phylogenomic databases
eggNOGi | KOG1818, Eukaryota |
GeneTreei | ENSGT00940000170673 |
HOGENOMi | CLU_011862_2_0_1 |
InParanoidi | P40343 |
OMAi | VCSREFM |
Miscellaneous databases
EvolutionaryTracei | P40343 |
PROi | PR:P40343 |
RNActi | P40343, protein |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR008942, ENTH_VHS IPR017073, Ubi-bd_Hrs_VPS27 IPR003903, UIM_dom IPR002014, VHS_dom IPR000306, Znf_FYVE IPR017455, Znf_FYVE-rel IPR011011, Znf_FYVE_PHD IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363, FYVE, 1 hit PF02809, UIM, 2 hits PF00790, VHS, 1 hit |
PIRSFi | PIRSF036956, Hrs_Vps27, 1 hit |
SMARTi | View protein in SMART SM00064, FYVE, 1 hit SM00726, UIM, 2 hits SM00288, VHS, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit SSF57903, SSF57903, 1 hit |
PROSITEi | View protein in PROSITE PS50330, UIM, 2 hits PS50179, VHS, 1 hit PS50178, ZF_FYVE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS27_YEAST | |
Accessioni | P40343Primary (citable) accession number: P40343 Secondary accession number(s): D6W1I1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 5, 2010 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families