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UniProtKB - P40341 (YTA12_YEAST)
Protein
Mitochondrial respiratory chain complexes assembly protein YTA12
Gene
YTA12
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides.
2 PublicationsMiscellaneous
Present with 11500 molecules/cell in log phase SD medium.1 Publication
Cofactori
Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 613 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 614 | By similarity | 1 | |
Metal bindingi | 617 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 689 | Zinc; catalyticBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 388 – 395 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: SGD
- ATP-dependent peptidase activity Source: InterPro
- ATP hydrolysis activity Source: SGD
- metalloendopeptidase activity Source: InterPro
- metallopeptidase activity Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular protein-containing complex assembly Source: SGD
- mitochondrial protein processing Source: GO_Central
- protein catabolic process Source: ComplexPortal
- protein-containing complex assembly Source: ComplexPortal
- proteolysis Source: SGD
- signal peptide processing Source: SGD
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.24.B18, 984 |
Protein family/group databases
MEROPSi | M41.003 |
TCDBi | 3.A.29.1.1, the mitochondrial inner membrane i-aaa protease complex (mimp) familly |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial respiratory chain complexes assembly protein YTA12 (EC:3.4.24.-)Alternative name(s): Tat-binding homolog 12 |
Gene namesi | Name:YTA12 Synonyms:RCA1 Ordered Locus Names:YMR089C ORF Names:YM9582.14C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004695, YTA12 |
VEuPathDBi | FungiDB:YMR089C |
Subcellular locationi
Mitochondrion
- Mitochondrion membrane 1 Publication; Multi-pass membrane protein 1 Publication
Mitochondrion
- m-AAA complex Source: SGD
- mitochondrial inner boundary membrane Source: SGD
- mitochondrial inner membrane Source: SGD
- mitochondrion Source: SGD
Other locations
- cytoplasm Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 178 – 194 | HelicalSequence analysisAdd BLAST | 17 | |
Transmembranei | 294 – 311 | HelicalSequence analysisAdd BLAST | 18 |
Keywords - Cellular componenti
Membrane, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 614 | E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084676 | 1 – 825 | Mitochondrial respiratory chain complexes assembly protein YTA12Add BLAST | 825 |
Proteomic databases
MaxQBi | P40341 |
PaxDbi | P40341 |
PRIDEi | P40341 |
Interactioni
Subunit structurei
Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.
1 PublicationBinary interactionsi
P40341
With | #Exp. | IntAct |
---|---|---|
AFG3 [P39925] | 3 | EBI-14858,EBI-2317 |
Protein-protein interaction databases
BioGRIDi | 35264, 188 interactors |
ComplexPortali | CPX-1654, m-AAA complex |
DIPi | DIP-889N |
IntActi | P40341, 9 interactors |
MINTi | P40341 |
STRINGi | 4932.YMR089C |
Miscellaneous databases
RNActi | P40341, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 43 – 67 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 115 – 173 | DisorderedSequence analysisAdd BLAST | 59 | |
Regioni | 806 – 825 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 46 – 67 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 115 – 136 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 152 – 170 | Pro residuesSequence analysisAdd BLAST | 19 |
Sequence similaritiesi
In the N-terminal section; belongs to the AAA ATPase family.Curated
In the C-terminal section; belongs to the peptidase M41 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0731, Eukaryota |
HOGENOMi | CLU_000688_23_0_1 |
InParanoidi | P40341 |
OMAi | WQEFRNT |
Family and domain databases
Gene3Di | 1.20.58.760, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01458, FtsH, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR005936, FtsH IPR027417, P-loop_NTPase IPR011546, Pept_M41_FtsH_extracell IPR000642, Peptidase_M41 IPR037219, Peptidase_M41-like |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF06480, FtsH_ext, 1 hit PF01434, Peptidase_M41, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF140990, SSF140990, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01241, FtsH_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
i Sequence
Sequence statusi: Complete.
P40341-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK
60 70 80 90 100
NEDNNEDAKL NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI
110 120 130 140 150
DESIRRLEDA VLKQESNRIQ EERKEKEEEN GPSKAKSNRT KEQGYFEGNN
160 170 180 190 200
SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL FQIGLTFFLL SFLLDLLNSL
210 220 230 240 250
EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG KNQADNYGRN
260 270 280 290 300
FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP
310 320 330 340 350
TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV
360 370 380 390 400
AGCDEAKEEI MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA
410 420 430 440 450
TAGEAGVPFY FVSGSEFVEM FVGVGAARVR DLFKTARENA PSIVFIDEID
460 470 480 490 500
AIGKARQKGN FSGANDEREN TLNQMLVEMD GFTPADHVVV LAGTNRPDIL
510 520 530 540 550
DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE IFDLKNRLAA
560 570 580 590 600
LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK
610 620 630 640 650
LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP
660 670 680 690 700
GDIFLLTEQQ LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM
710 720 730 740 750
VTELGMSDKI GWVNYQKRDD SDLTKPFSDE TGDIIDSEVY RIVQECHDRC
760 770 780 790 800
TKLLKEKAED VEKIAQVLLK KEVLTREDMI DLLGKRPFPE RNDAFDKYLN
810 820
DYETEKIRKE EEKNEKRNEP KPSTN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | D → G in AAT93118 (PubMed:17322287).Curated | 1 | |
Sequence conflicti | 349 – 350 | DV → EL in CAA56955 (PubMed:7754704).Curated | 2 | |
Sequence conflicti | 653 | I → V in AAA62606 (PubMed:7929327).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09358 Genomic DNA Translation: AAA62606.1 X81068 Genomic DNA Translation: CAA56955.1 Z49259 Genomic DNA Translation: CAA89236.1 AY693099 Genomic DNA Translation: AAT93118.1 BK006946 Genomic DNA Translation: DAA09986.1 |
PIRi | S54465 |
RefSeqi | NP_013807.1, NM_001182589.1 |
Genome annotation databases
EnsemblFungii | YMR089C_mRNA; YMR089C; YMR089C |
GeneIDi | 855114 |
KEGGi | sce:YMR089C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09358 Genomic DNA Translation: AAA62606.1 X81068 Genomic DNA Translation: CAA56955.1 Z49259 Genomic DNA Translation: CAA89236.1 AY693099 Genomic DNA Translation: AAT93118.1 BK006946 Genomic DNA Translation: DAA09986.1 |
PIRi | S54465 |
RefSeqi | NP_013807.1, NM_001182589.1 |
3D structure databases
SMRi | P40341 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35264, 188 interactors |
ComplexPortali | CPX-1654, m-AAA complex |
DIPi | DIP-889N |
IntActi | P40341, 9 interactors |
MINTi | P40341 |
STRINGi | 4932.YMR089C |
Protein family/group databases
MEROPSi | M41.003 |
TCDBi | 3.A.29.1.1, the mitochondrial inner membrane i-aaa protease complex (mimp) familly |
Proteomic databases
MaxQBi | P40341 |
PaxDbi | P40341 |
PRIDEi | P40341 |
Genome annotation databases
EnsemblFungii | YMR089C_mRNA; YMR089C; YMR089C |
GeneIDi | 855114 |
KEGGi | sce:YMR089C |
Organism-specific databases
SGDi | S000004695, YTA12 |
VEuPathDBi | FungiDB:YMR089C |
Phylogenomic databases
eggNOGi | KOG0731, Eukaryota |
HOGENOMi | CLU_000688_23_0_1 |
InParanoidi | P40341 |
OMAi | WQEFRNT |
Enzyme and pathway databases
BRENDAi | 3.4.24.B18, 984 |
Miscellaneous databases
PROi | PR:P40341 |
RNActi | P40341, protein |
Family and domain databases
Gene3Di | 1.20.58.760, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_01458, FtsH, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR005936, FtsH IPR027417, P-loop_NTPase IPR011546, Pept_M41_FtsH_extracell IPR000642, Peptidase_M41 IPR037219, Peptidase_M41-like |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF06480, FtsH_ext, 1 hit PF01434, Peptidase_M41, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF140990, SSF140990, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01241, FtsH_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | YTA12_YEAST | |
Accessioni | P40341Primary (citable) accession number: P40341 Secondary accession number(s): D6VZR2, E9P917 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families