UniProtKB - P40339 (RFC4_YEAST)
Replication factor C subunit 4
RFC4
Functioni
Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.
3 PublicationsMiscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | ATP; via carbonyl oxygen | 1 | |
Binding sitei | 24 | ATP; via amide nitrogen | 1 | |
Binding sitei | 145 | ATP | 1 | |
Binding sitei | 203 | ATP | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 49 – 57 | ATP | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- DNA clamp unloading Source: ComplexPortal
- DNA damage checkpoint signaling Source: ComplexPortal
- DNA-dependent DNA replication Source: ComplexPortal
- DNA repair Source: GO_Central
- leading strand elongation Source: SGD
- mitotic sister chromatid cohesion Source: ComplexPortal
- sister chromatid cohesion Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-110312, Translesion synthesis by REV1 R-SCE-110314, Recognition of DNA damage by PCNA-containing replication complex R-SCE-110320, Translesion Synthesis by POLH R-SCE-176187, Activation of ATR in response to replication stress R-SCE-5655862, Translesion synthesis by POLK R-SCE-5656121, Translesion synthesis by POLI R-SCE-5656169, Termination of translesion DNA synthesis R-SCE-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091, Polymerase switching |
Names & Taxonomyi
Protein namesi | Recommended name: Replication factor C subunit 4Short name: Replication factor C4 Alternative name(s): Activator 1 37 kDa subunit |
Gene namesi | Name:RFC4 Ordered Locus Names:YOL094C ORF Names:O0923 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005454, RFC4 |
VEuPathDBi | FungiDB:YOL094C |
Subcellular locationi
Nucleus
- Nucleus Curated
Cytosol
- cytosol Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- Ctf18 RFC-like complex Source: SGD
- DNA replication factor C complex Source: SGD
- Elg1 RFC-like complex Source: SGD
- Rad17 RFC-like complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121771 | 1 – 323 | Replication factor C subunit 4Add BLAST | 323 |
Proteomic databases
MaxQBi | P40339 |
PaxDbi | P40339 |
PRIDEi | P40339 |
Interactioni
Subunit structurei
Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP.
Component of the RAD24-RFC complex which consists of RAD14, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex.
Component of the ELG1-RFC complex which consists of ELG1, RFC2, RFC3, RFC4 and RFC5.
Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. RFC4 interacts with ECO1.
8 PublicationsBinary interactionsi
P40339
With | #Exp. | IntAct |
---|---|---|
CTF18 [P49956] | 5 | EBI-15009,EBI-4560 |
CTF8 [P38877] | 2 | EBI-15009,EBI-5216 |
ELG1 [Q12050] | 5 | EBI-15009,EBI-32195 |
RAD24 [P32641] | 2 | EBI-15009,EBI-14675 |
Protein-protein interaction databases
BioGRIDi | 34308, 333 interactors |
ComplexPortali | CPX-1731, CTF18-RFC complex CPX-1807, Rad17 RFC-like complex CPX-422, ELG1-RFC complex CPX-545, DNA replication factor C complex |
DIPi | DIP-2530N |
IntActi | P40339, 47 interactors |
MINTi | P40339 |
STRINGi | 4932.YOL094C |
Miscellaneous databases
RNActi | P40339, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P40339 |
SMRi | P40339 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40339 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0991, Eukaryota |
GeneTreei | ENSGT00550000075050 |
HOGENOMi | CLU_042324_0_1_1 |
InParanoidi | P40339 |
OMAi | SCNYSSQ |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR008921, DNA_pol3_clamp-load_cplx_C IPR027417, P-loop_NTPase IPR013748, Rep_factorC_C |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF08542, Rep_fac_C, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKTLSLQLP WVEKYRPQVL SDIVGNKETI DRLQQIAKDG NMPHMIISGM
60 70 80 90 100
PGIGKTTSVH CLAHELLGRS YADGVLELNA SDDRGIDVVR NQIKHFAQKK
110 120 130 140 150
LHLPPGKHKI VILDEADSMT AGAQQALRRT MELYSNSTRF AFACNQSNKI
160 170 180 190 200
IEPLQSRCAI LRYSKLSDED VLKRLLQIIK LEDVKYTNDG LEAIIFTAEG
210 220 230 240 250
DMRQAINNLQ STVAGHGLVN ADNVFKIVDS PHPLIVKKML LASNLEDSIQ
260 270 280 290 300
ILRTDLWKKG YSSIDIVTTS FRVTKNLAQV KESVRLEMIK EIGLTHMRIL
310 320
EGVGTYLQLA SMLAKIHKLN NKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L20502 Genomic DNA Translation: AAA34970.1 U26030 Genomic DNA Translation: AAC49063.1 X83121 Genomic DNA Translation: CAA58185.1 Z74836 Genomic DNA Translation: CAA99106.1 BK006948 Genomic DNA Translation: DAA10690.1 |
PIRi | A53845 |
RefSeqi | NP_014547.1, NM_001183348.1 |
Genome annotation databases
EnsemblFungii | YOL094C_mRNA; YOL094C; YOL094C |
GeneIDi | 854059 |
KEGGi | sce:YOL094C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L20502 Genomic DNA Translation: AAA34970.1 U26030 Genomic DNA Translation: AAC49063.1 X83121 Genomic DNA Translation: CAA58185.1 Z74836 Genomic DNA Translation: CAA99106.1 BK006948 Genomic DNA Translation: DAA10690.1 |
PIRi | A53845 |
RefSeqi | NP_014547.1, NM_001183348.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SXJ | X-ray | 2.85 | B | 1-323 | [»] | |
AlphaFoldDBi | P40339 | |||||
SMRi | P40339 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34308, 333 interactors |
ComplexPortali | CPX-1731, CTF18-RFC complex CPX-1807, Rad17 RFC-like complex CPX-422, ELG1-RFC complex CPX-545, DNA replication factor C complex |
DIPi | DIP-2530N |
IntActi | P40339, 47 interactors |
MINTi | P40339 |
STRINGi | 4932.YOL094C |
Proteomic databases
MaxQBi | P40339 |
PaxDbi | P40339 |
PRIDEi | P40339 |
Genome annotation databases
EnsemblFungii | YOL094C_mRNA; YOL094C; YOL094C |
GeneIDi | 854059 |
KEGGi | sce:YOL094C |
Organism-specific databases
SGDi | S000005454, RFC4 |
VEuPathDBi | FungiDB:YOL094C |
Phylogenomic databases
eggNOGi | KOG0991, Eukaryota |
GeneTreei | ENSGT00550000075050 |
HOGENOMi | CLU_042324_0_1_1 |
InParanoidi | P40339 |
OMAi | SCNYSSQ |
Enzyme and pathway databases
Reactomei | R-SCE-110312, Translesion synthesis by REV1 R-SCE-110314, Recognition of DNA damage by PCNA-containing replication complex R-SCE-110320, Translesion Synthesis by POLH R-SCE-176187, Activation of ATR in response to replication stress R-SCE-5655862, Translesion synthesis by POLK R-SCE-5656121, Translesion synthesis by POLI R-SCE-5656169, Termination of translesion DNA synthesis R-SCE-5696397, Gap-filling DNA repair synthesis and ligation in GG-NER R-SCE-6782135, Dual incision in TC-NER R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091, Polymerase switching |
Miscellaneous databases
EvolutionaryTracei | P40339 |
PROi | PR:P40339 |
RNActi | P40339, protein |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR008921, DNA_pol3_clamp-load_cplx_C IPR027417, P-loop_NTPase IPR013748, Rep_factorC_C |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF08542, Rep_fac_C, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RFC4_YEAST | |
Accessioni | P40339Primary (citable) accession number: P40339 Secondary accession number(s): D6W1X4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families