UniProtKB - P40336 (VP26A_MOUSE)
Vacuolar protein sorting-associated protein 26A
Vps26a
Functioni
GO - Biological processi
- intracellular protein transport Source: GO_Central
- retrograde transport, endosome to Golgi Source: MGI
- retrograde transport, endosome to plasma membrane Source: UniProtKB
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-3238698, WNT ligand biogenesis and trafficking |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 26AAlternative name(s): H<beta>58 protein Short name: H beta 58 Vesicle protein sorting 26A Short name: mVPS26 |
Gene namesi | Name:Vps26a Synonyms:Vps26 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1353654, Vps26a |
Subcellular locationi
Endosome
- Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication
- Early endosome 2 Publications
Other locations
- Cytoplasm 1 Publication
Note: Predominantly found in early not late endosomes.1 Publication
Cytosol
- cytosol Source: MGI
Endosome
- early endosome Source: UniProtKB
- endosome Source: MGI
- endosome membrane Source: MGI
- tubular endosome Source: UniProtKB
Lysosome
- lysosome Source: MGI
Other locations
- retromer complex Source: UniProtKB
- retromer, cargo-selective complex Source: MGI
- vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 44 | D → A: Decreases interaction with SNX27. 1 Publication | 1 | |
Mutagenesisi | 154 | L → A: Decreases interaction with SNX27. 1 Publication | 1 | |
Mutagenesisi | 235 – 236 | IM → DN: Disrupts interaction with VPS35:VPS29 dimer; no endosomal localization. | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000073008 | 1 – 327 | Vacuolar protein sorting-associated protein 26AAdd BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 315 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P40336 |
jPOSTi | P40336 |
MaxQBi | P40336 |
PaxDbi | P40336 |
PeptideAtlasi | P40336 |
PRIDEi | P40336 |
PTM databases
iPTMneti | P40336 |
PhosphoSitePlusi | P40336 |
SwissPalmi | P40336 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020078, Expressed in spermatocyte and 324 other tissues |
Genevisiblei | P40336, MM |
Interactioni
Subunit structurei
Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35 (PubMed:18088321, PubMed:20875039). The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (Probable). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex (By similarity).
Interacts with VPS29, VPS35, SNX27 (PubMed:18088321, PubMed:20875039, PubMed:25136126).
Interacts with SNX1, SNX2, SNX5, SNX6, SNX3, RAB7A, ECPAS, EHD1, WASHC5, SORL1 (By similarity).
1 PublicationBy similarity3 PublicationsBinary interactionsi
Isoform 1 [P40336-1]
With | #Exp. | IntAct |
---|---|---|
Vps35 [Q9EQH3] | 2 | EBI-15553779,EBI-775825 |
Protein-protein interaction databases
BioGRIDi | 206007, 21 interactors |
CORUMi | P40336 |
DIPi | DIP-32041N |
IntActi | P40336, 5 interactors |
MINTi | P40336 |
STRINGi | 10090.ENSMUSP00000090130 |
Miscellaneous databases
RNActi | P40336, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P40336 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3063, Eukaryota |
GeneTreei | ENSGT00950000183064 |
HOGENOMi | CLU_031077_0_0_1 |
InParanoidi | P40336 |
OMAi | KPRTNFH |
OrthoDBi | 987411at2759 |
PhylomeDBi | P40336 |
TreeFami | TF300907 |
Family and domain databases
Gene3Di | 2.60.40.640, 2 hits |
InterProi | View protein in InterPro IPR014752, Arrestin_C IPR028934, Vps26-related |
PANTHERi | PTHR12233, PTHR12233, 1 hit |
Pfami | View protein in Pfam PF03643, Vps26, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSFLGGFFGP ICEIDVALND GETRKMAEMK TEDGKVEKHY LFYDGESVSG
60 70 80 90 100
KVNLAFKQPG KRLEHQGIRI EFVGQIELFN DKSNTHEFVN LVKELALPGE
110 120 130 140 150
LTQSRSYDFE FMQVEKPYES YIGANVRLRY FLKVTIVRRL TDLVKEYDLI
160 170 180 190 200
VHQLATYPDV NNSIKMEVGI EDCLHIEFEY NKSKYHLKDV IVGKIYFLLV
210 220 230 240 250
RIKIQHMELQ LIKKEITGIG PSTTTETETI AKYEIMDGAP VKGESIPIRL
260 270 280 290 300
FLAGYDPTPT MRDVNKKFSV RYFLNLVLVD EEDRRYFKQQ EIILWRKAPE
310 320
KLRKQRTNFH QRFESPDSQA SAEQPEM
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P7Z9 | A0A1W2P7Z9_MOUSE | Vacuolar protein sorting-associated... | Vps26a | 123 | Annotation score: | ||
A0A1W2P7R7 | A0A1W2P7R7_MOUSE | Vacuolar protein sorting-associated... | Vps26a | 63 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 | E → Q in BAC25745 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 71 | E → G in BAE35367 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 79 | F → L in BAE40171 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 188 | K → R in BAE40415 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019926 | 1 | M → MSEPLPAFLDRLWGPWLGTR SPPSRSSAASPSK in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S41204 mRNA Translation: AAB22718.1 AK028096 mRNA Translation: BAC25745.1 AK150244 mRNA Translation: BAE29408.1 AK147989 mRNA Translation: BAE28271.1 AK151360 mRNA Translation: BAE30335.1 AK159783 mRNA Translation: BAE35367.1 AK167592 mRNA Translation: BAE39650.1 AK165853 mRNA Translation: BAE38415.1 AK168213 mRNA Translation: BAE40171.1 AK168537 mRNA Translation: BAE40415.1 BC007148 mRNA Translation: AAH07148.1 |
CCDSi | CCDS35921.1 [P40336-1] CCDS48579.1 [P40336-2] |
PIRi | A44882 B44882 |
RefSeqi | NP_001106826.1, NM_001113355.1 [P40336-2] NP_598433.1, NM_133672.3 [P40336-1] |
Genome annotation databases
Ensembli | ENSMUST00000092473; ENSMUSP00000090130; ENSMUSG00000020078 [P40336-2] ENSMUST00000105447; ENSMUSP00000101087; ENSMUSG00000020078 [P40336-1] |
GeneIDi | 30930 |
KEGGi | mmu:30930 |
UCSCi | uc007fhf.2, mouse [P40336-2] uc007fhg.2, mouse [P40336-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S41204 mRNA Translation: AAB22718.1 AK028096 mRNA Translation: BAC25745.1 AK150244 mRNA Translation: BAE29408.1 AK147989 mRNA Translation: BAE28271.1 AK151360 mRNA Translation: BAE30335.1 AK159783 mRNA Translation: BAE35367.1 AK167592 mRNA Translation: BAE39650.1 AK165853 mRNA Translation: BAE38415.1 AK168213 mRNA Translation: BAE40171.1 AK168537 mRNA Translation: BAE40415.1 BC007148 mRNA Translation: AAH07148.1 |
CCDSi | CCDS35921.1 [P40336-1] CCDS48579.1 [P40336-2] |
PIRi | A44882 B44882 |
RefSeqi | NP_001106826.1, NM_001113355.1 [P40336-2] NP_598433.1, NM_133672.3 [P40336-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4P2A | X-ray | 2.70 | A | 9-327 | [»] | |
6VAC | electron microscopy | 5.70 | B | 1-327 | [»] | |
SMRi | P40336 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 206007, 21 interactors |
CORUMi | P40336 |
DIPi | DIP-32041N |
IntActi | P40336, 5 interactors |
MINTi | P40336 |
STRINGi | 10090.ENSMUSP00000090130 |
PTM databases
iPTMneti | P40336 |
PhosphoSitePlusi | P40336 |
SwissPalmi | P40336 |
Proteomic databases
EPDi | P40336 |
jPOSTi | P40336 |
MaxQBi | P40336 |
PaxDbi | P40336 |
PeptideAtlasi | P40336 |
PRIDEi | P40336 |
Protocols and materials databases
Antibodypediai | 28695, 193 antibodies |
DNASUi | 30930 |
Genome annotation databases
Ensembli | ENSMUST00000092473; ENSMUSP00000090130; ENSMUSG00000020078 [P40336-2] ENSMUST00000105447; ENSMUSP00000101087; ENSMUSG00000020078 [P40336-1] |
GeneIDi | 30930 |
KEGGi | mmu:30930 |
UCSCi | uc007fhf.2, mouse [P40336-2] uc007fhg.2, mouse [P40336-1] |
Organism-specific databases
CTDi | 9559 |
MGIi | MGI:1353654, Vps26a |
Phylogenomic databases
eggNOGi | KOG3063, Eukaryota |
GeneTreei | ENSGT00950000183064 |
HOGENOMi | CLU_031077_0_0_1 |
InParanoidi | P40336 |
OMAi | KPRTNFH |
OrthoDBi | 987411at2759 |
PhylomeDBi | P40336 |
TreeFami | TF300907 |
Enzyme and pathway databases
Reactomei | R-MMU-3238698, WNT ligand biogenesis and trafficking |
Miscellaneous databases
BioGRID-ORCSi | 30930, 1 hit in 19 CRISPR screens |
ChiTaRSi | Vps26a, mouse |
PROi | PR:P40336 |
RNActi | P40336, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020078, Expressed in spermatocyte and 324 other tissues |
Genevisiblei | P40336, MM |
Family and domain databases
Gene3Di | 2.60.40.640, 2 hits |
InterProi | View protein in InterPro IPR014752, Arrestin_C IPR028934, Vps26-related |
PANTHERi | PTHR12233, PTHR12233, 1 hit |
Pfami | View protein in Pfam PF03643, Vps26, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VP26A_MOUSE | |
Accessioni | P40336Primary (citable) accession number: P40336 Secondary accession number(s): Q3TGY3 Q8C1E9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families