UniProtKB - P40327 (PRS4_YEAST)
Protein
26S proteasome regulatory subunit 4 homolog
Gene
RPT2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). Has ATPase activity.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 223 – 230 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: UniProtKB-KW
- proteasome-activating ATPase activity Source: SGD
- TBP-class protein binding Source: GO_Central
GO - Biological processi
- cellular protein-containing complex localization Source: SGD
- nonfunctional rRNA decay Source: SGD
- peptide catabolic process Source: SGD
- positive regulation of protein catabolic process Source: SGD
- proteasome regulatory particle assembly Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit 4 homologAlternative name(s): Tat-binding homolog 5 |
Gene namesi | Name:RPT2 Synonyms:YHS4, YTA5 Ordered Locus Names:YDL007W ORF Names:D2920 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002165, RPT2 |
VEuPathDBi | FungiDB:YDL007W |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: UniProtKB-SubCell
- proteasome regulatory particle, base subcomplex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 229 | K → Q: 73% loss of ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000084685 | 2 – 437 | 26S proteasome regulatory subunit 4 homologAdd BLAST | 436 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Cross-linki | 234 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 255 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 290 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Lipoprotein, Myristate, Ubl conjugationProteomic databases
MaxQBi | P40327 |
PaxDbi | P40327 |
PRIDEi | P40327 |
PTM databases
iPTMneti | P40327 |
Interactioni
Binary interactionsi
Hide detailsP40327
With | #Exp. | IntAct |
---|---|---|
RPN1 [P38764] | 6 | EBI-13901,EBI-15913 |
RPN11 [P43588] | 2 | EBI-13901,EBI-11219 |
RPT1 [P33299] | 8 | EBI-13901,EBI-13910 |
GO - Molecular functioni
- TBP-class protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 32047, 348 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-6282N |
IntActi | P40327, 38 interactors |
MINTi | P40327 |
STRINGi | 4932.YDL007W |
Miscellaneous databases
RNActi | P40327, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the AAA ATPase family.Curated
Phylogenomic databases
eggNOGi | KOG0726, Eukaryota |
GeneTreei | ENSGT00990000203571 |
HOGENOMi | CLU_000688_2_3_1 |
InParanoidi | P40327 |
OMAi | ARCKLRY |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P40327-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGQGVSSGQD KKKKKGSNQK PKYEPPVQSK FGRKKRKGGP ATAEKLPNIY
60 70 80 90 100
PSTRCKLKLL RMERIKDHLL LEEEFVSNSE ILKPFEKKQE EEKKQLEEIR
110 120 130 140 150
GNPLSIGTLE EIIDDDHAIV TSPTMPDYYV SILSFVDKEL LEPGCSVLLH
160 170 180 190 200
HKTMSIVGVL QDDADPMVSV MKMDKSPTES YSDIGGLESQ IQEIKESVEL
210 220 230 240 250
PLTHPELYEE MGIKPPKGVI LYGAPGTGKT LLAKAVANQT SATFLRIVGS
260 270 280 290 300
ELIQKYLGDG PRLCRQIFKV AGENAPSIVF IDEIDAIGTK RYDSNSGGER
310 320 330 340 350
EIQRTMLELL NQLDGFDDRG DVKVIMATNK IETLDPALIR PGRIDRKILF
360 370 380 390 400
ENPDLSTKKK ILGIHTSKMN LSEDVNLETL VTTKDDLSGA DIQAMCTEAG
410 420 430
LLALRERRMQ VTAEDFKQAK ERVMKNKVEE NLEGLYL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 347 | K → N in AAA97498 (PubMed:7721833).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X81070 Genomic DNA Translation: CAA56957.1 L17040 Genomic DNA Translation: AAA97498.1 Z48432 Genomic DNA Translation: CAA88352.1 Z74055 Genomic DNA Translation: CAA98563.1 BK006938 Genomic DNA Translation: DAA11841.1 |
PIRi | S46613 |
RefSeqi | NP_010277.1, NM_001180066.1 |
Genome annotation databases
EnsemblFungii | YDL007W_mRNA; YDL007W; YDL007W |
GeneIDi | 851557 |
KEGGi | sce:YDL007W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X81070 Genomic DNA Translation: CAA56957.1 L17040 Genomic DNA Translation: AAA97498.1 Z48432 Genomic DNA Translation: CAA88352.1 Z74055 Genomic DNA Translation: CAA98563.1 BK006938 Genomic DNA Translation: DAA11841.1 |
PIRi | S46613 |
RefSeqi | NP_010277.1, NM_001180066.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCO | electron microscopy | 4.80 | I | 1-437 | [»] | |
3JCP | electron microscopy | 4.60 | I | 1-437 | [»] | |
4CR2 | electron microscopy | 7.70 | I | 1-437 | [»] | |
4CR3 | electron microscopy | 9.30 | I | 1-437 | [»] | |
4CR4 | electron microscopy | 8.80 | I | 1-437 | [»] | |
5A5B | electron microscopy | 9.50 | I | 1-437 | [»] | |
5MP9 | electron microscopy | 4.10 | I | 1-437 | [»] | |
5MPA | electron microscopy | 4.50 | I | 1-437 | [»] | |
5MPB | electron microscopy | 7.80 | I | 1-437 | [»] | |
5MPC | electron microscopy | 7.70 | I | 1-437 | [»] | |
5WVI | electron microscopy | 6.30 | I | 1-437 | [»] | |
5WVK | electron microscopy | 4.20 | I | 1-437 | [»] | |
6EF0 | electron microscopy | 4.43 | I | 166-436 | [»] | |
6EF1 | electron microscopy | 4.73 | I | 167-437 | [»] | |
6EF2 | electron microscopy | 4.27 | I | 178-437 | [»] | |
6EF3 | electron microscopy | 4.17 | I | 1-437 | [»] | |
6FVT | electron microscopy | 4.10 | I | 53-437 | [»] | |
6FVU | electron microscopy | 4.50 | I | 54-437 | [»] | |
6FVV | electron microscopy | 5.40 | I | 53-437 | [»] | |
6FVW | electron microscopy | 4.50 | I | 53-437 | [»] | |
6FVX | electron microscopy | 4.90 | I | 53-437 | [»] | |
6FVY | electron microscopy | 6.10 | I | 53-437 | [»] | |
6J2C | electron microscopy | 7.00 | I | 1-437 | [»] | |
6J2N | electron microscopy | 7.50 | I | 1-437 | [»] | |
6J2Q | electron microscopy | 3.80 | I | 1-437 | [»] | |
6J2X | electron microscopy | 3.80 | I | 1-437 | [»] | |
6J30 | electron microscopy | 4.50 | I | 1-437 | [»] | |
SMRi | P40327 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32047, 348 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-6282N |
IntActi | P40327, 38 interactors |
MINTi | P40327 |
STRINGi | 4932.YDL007W |
PTM databases
iPTMneti | P40327 |
Proteomic databases
MaxQBi | P40327 |
PaxDbi | P40327 |
PRIDEi | P40327 |
Genome annotation databases
EnsemblFungii | YDL007W_mRNA; YDL007W; YDL007W |
GeneIDi | 851557 |
KEGGi | sce:YDL007W |
Organism-specific databases
SGDi | S000002165, RPT2 |
VEuPathDBi | FungiDB:YDL007W |
Phylogenomic databases
eggNOGi | KOG0726, Eukaryota |
GeneTreei | ENSGT00990000203571 |
HOGENOMi | CLU_000688_2_3_1 |
InParanoidi | P40327 |
OMAi | ARCKLRY |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases |
Miscellaneous databases
PROi | PR:P40327 |
RNActi | P40327, protein |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRS4_YEAST | |
Accessioni | P40327Primary (citable) accession number: P40327 Secondary accession number(s): D6VRY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 192 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families