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Protein

Proteasome subunit beta type-10

Gene

PSMB10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease
Biological processHost-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-HSA-1236974 ER-Phagosome pathway
R-HSA-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174113 SCF-beta-TrCP mediated degradation of Emi1
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-180534 Vpu mediated degradation of CD4
R-HSA-180585 Vif-mediated degradation of APOBEC3G
R-HSA-187577 SCF(Skp2)-mediated degradation of p27/p21
R-HSA-195253 Degradation of beta-catenin by the destruction complex
R-HSA-202424 Downstream TCR signaling
R-HSA-211733 Regulation of activated PAK-2p34 by proteasome mediated degradation
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-382556 ABC-family proteins mediated transport
R-HSA-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-HSA-4608870 Asymmetric localization of PCP proteins
R-HSA-4641257 Degradation of AXIN
R-HSA-4641258 Degradation of DVL
R-HSA-5358346 Hedgehog ligand biogenesis
R-HSA-5362768 Hh mutants that don't undergo autocatalytic processing are degraded by ERAD
R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5610780 Degradation of GLI1 by the proteasome
R-HSA-5610783 Degradation of GLI2 by the proteasome
R-HSA-5610785 GLI3 is processed to GLI3R by the proteasome
R-HSA-5632684 Hedgehog 'on' state
R-HSA-5658442 Regulation of RAS by GAPs
R-HSA-5668541 TNFR2 non-canonical NF-kB pathway
R-HSA-5676590 NIK-->noncanonical NF-kB signaling
R-HSA-5678895 Defective CFTR causes cystic fibrosis
R-HSA-5687128 MAPK6/MAPK4 signaling
R-HSA-5689603 UCH proteinases
R-HSA-5689880 Ub-specific processing proteases
R-HSA-68827 CDT1 association with the CDC6:ORC:origin complex
R-HSA-68949 Orc1 removal from chromatin
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-69229 Ubiquitin-dependent degradation of Cyclin D1
R-HSA-69481 G2/M Checkpoints
R-HSA-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-HSA-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-HSA-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-HSA-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
R-HSA-8939902 Regulation of RUNX2 expression and activity
R-HSA-8941858 Regulation of RUNX3 expression and activity
R-HSA-8948751 Regulation of PTEN stability and activity
R-HSA-8951664 Neddylation
R-HSA-9010553 Regulation of expression of SLITs and ROBOs
R-HSA-9020702 Interleukin-1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.014

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-10 (EC:3.4.25.1)
Alternative name(s):
Low molecular mass protein 10
Macropain subunit MECl-1
Multicatalytic endopeptidase complex subunit MECl-1
Proteasome MECl-1
Proteasome subunit beta-2i
Gene namesi
Name:PSMB10
Synonyms:LMP10, MECL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000205220.11
HGNCiHGNC:9538 PSMB10
MIMi176847 gene
neXtProtiNX_P40306

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5699
OpenTargetsiENSG00000205220
PharmGKBiPA33883

Chemistry databases

ChEMBLiCHEMBL3317334
DrugBankiDB08889 Carfilzomib

Polymorphism and mutation databases

BioMutaiPSMB10
DMDMi730376

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266511 – 39Removed in mature formBy similarityAdd BLAST39
ChainiPRO_000002665240 – 273Proteasome subunit beta type-10Add BLAST234

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei230PhosphoserineCombined sources1

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei39 – 40Cleavage; by autolysisBy similarity2

Keywords - PTMi

Acetylation, Phosphoprotein, Zymogen

Proteomic databases

EPDiP40306
MaxQBiP40306
PaxDbiP40306
PeptideAtlasiP40306
PRIDEiP40306
ProteomicsDBi55360

2D gel databases

OGPiP40306
SWISS-2DPAGEiP40306

PTM databases

iPTMnetiP40306
PhosphoSitePlusiP40306

Expressioni

Developmental stagei

Highly expressed in immature dendritic cells (at protein level).1 Publication

Inductioni

Up-regulated by IFNG/IFN-gamma (at protein level). Up-regulated by IRF1. Up-regulated by TNF (at protein level). Up-regulated by tetrodotoxin (TTX) in glial cells. Up-regulated in Crohn's bowel disease (CD). Up-regulated by CD40L via the NFKB1 pathway in cancer cells.7 Publications

Gene expression databases

BgeeiENSG00000205220
CleanExiHS_PSMB10
ExpressionAtlasiP40306 baseline and differential
GenevisibleiP40306 HS

Organism-specific databases

HPAiCAB034384
HPA030224
HPA030225

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB7. Component of the spermatoproteasome, a form of the proteasome specifically found in testis.1 Publication
(Microbial infection) Interacts with HIV-1 TAT protein.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi111672, 23 interactors
IntActiP40306, 11 interactors
MINTiP40306
STRINGi9606.ENSP00000351314

Chemistry databases

BindingDBiP40306

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6AVOelectron microscopy3.80B/E40-273[»]
ProteinModelPortaliP40306
SMRiP40306
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0173 Eukaryota
COG0638 LUCA
GeneTreeiENSGT00510000046533
HOGENOMiHOG000182856
HOVERGENiHBG093416
InParanoidiP40306
KOiK02733
OMAiQTLFRYR
OrthoDBiEOG091G0E5S
PhylomeDBiP40306
TreeFamiTF106222

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000243 Pept_T1A_subB
IPR034384 Proteasome_beta10
IPR024689 Proteasome_bsu_C
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PANTHERiPTHR11599:SF41 PTHR11599:SF41, 1 hit
PfamiView protein in Pfam
PF12465 Pr_beta_C, 1 hit
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPALEPRG GFSFENCQRN ASLERVLPGL KVPHARKTGT TIAGLVFQDG
60 70 80 90 100
VILGADTRAT NDSVVADKSC EKIHFIAPKI YCCGAGVAAD AEMTTRMVAS
110 120 130 140 150
KMELHALSTG REPRVATVTR ILRQTLFRYQ GHVGASLIVG GVDLTGPQLY
160 170 180 190 200
GVHPHGSYSR LPFTALGSGQ DAALAVLEDR FQPNMTLEAA QGLLVEAVTA
210 220 230 240 250
GILGDLGSGG NVDACVITKT GAKLLRTLSS PTEPVKRSGR YHFVPGTTAV
260 270
LTQTVKPLTL ELVEETVQAM EVE
Length:273
Mass (Da):28,936
Last modified:February 1, 1995 - v1
Checksum:iD6728E50513A45B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71874 Genomic DNA Translation: CAA50709.1
Y13640 mRNA Translation: CAA73982.1
BT019723 mRNA Translation: AAV38528.1
BT019724 mRNA Translation: AAV38529.1
AK312208 mRNA Translation: BAG35141.1
CH471092 Genomic DNA Translation: EAW83187.1
BC017198 mRNA Translation: AAH17198.1
BC052369 mRNA Translation: AAH52369.1
CCDSiCCDS10853.1
PIRiI38135
RefSeqiNP_002792.1, NM_002801.3
UniGeneiHs.9661

Genome annotation databases

EnsembliENST00000358514; ENSP00000351314; ENSG00000205220
GeneIDi5699
KEGGihsa:5699
UCSCiuc002eux.3 human

Similar proteinsi

Entry informationi

Entry nameiPSB10_HUMAN
AccessioniPrimary (citable) accession number: P40306
Secondary accession number(s): B2R5J4, Q5U098
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 187 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

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