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Protein

Proteasome subunit alpha type-4

Gene

PRE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.

Miscellaneous

Present with 16800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  • proteasomal ubiquitin-independent protein catabolic process Source: SGD
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciYEAST:G3O-33452-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-187577 SCF(Skp2)-mediated degradation of p27/p21
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-4 (EC:3.4.25.1)
Alternative name(s):
Macropain subunit PRE6
Multicatalytic endopeptidase complex subunit PRE6
Proteasome component PRE6
Proteinase YSCE subunit PRE6
Gene namesi
Name:PRE6
Ordered Locus Names:YOL038W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL038W
SGDiS000005398 PRE6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241651 – 254Proteasome subunit alpha type-4Add BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40303
PaxDbiP40303
PRIDEiP40303

PTM databases

iPTMnetiP40303

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with CIC1.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi34364, 276 interactors
ComplexPortaliCPX-2262 26S Proteasome complex
DIPiDIP-4844N
IntActiP40303, 52 interactors
MINTiP40303
STRINGi4932.YOL038W

Structurei

Secondary structure

1254
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Turni13 – 15Combined sources3
Helixi18 – 27Combined sources10
Beta strandi33 – 37Combined sources5
Beta strandi42 – 47Combined sources6
Turni57 – 59Combined sources3
Beta strandi63 – 68Combined sources6
Beta strandi71 – 77Combined sources7
Helixi79 – 100Combined sources22
Helixi106 – 119Combined sources14
Turni120 – 122Combined sources3
Beta strandi123 – 126Combined sources4
Beta strandi130 – 137Combined sources8
Beta strandi146 – 150Combined sources5
Turni152 – 154Combined sources3
Beta strandi156 – 165Combined sources10
Helixi168 – 176Combined sources9
Beta strandi181 – 183Combined sources3
Helixi188 – 203Combined sources16
Beta strandi204 – 207Combined sources4
Beta strandi210 – 216Combined sources7
Turni217 – 219Combined sources3
Beta strandi220 – 223Combined sources4
Helixi226 – 236Combined sources11
Helixi238 – 241Combined sources4

3D structure databases

ProteinModelPortaliP40303
SMRiP40303
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40303

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074753
HOGENOMiHOG000091085
InParanoidiP40303
KOiK02731
OMAiEVAIMEN
OrthoDBiEOG092C47D8

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR037560 Proteasome_alpha_7-like
IPR000426 Proteasome_asu_N
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
PANTHERiPTHR11599:SF15 PTHR11599:SF15, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P40303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGYDRALSI FSPDGHIFQV EYALEAVKRG TCAVGVKGKN CVVLGCERRS
60 70 80 90 100
TLKLQDTRIT PSKVSKIDSH VVLSFSGLNA DSRILIEKAR VEAQSHRLTL
110 120 130 140 150
EDPVTVEYLT RYVAGVQQRY TQSGGVRPFG VSTLIAGFDP RDDEPKLYQT
160 170 180 190 200
EPSGIYSSWS AQTIGRNSKT VREFLEKNYD RKEPPATVEE CVKLTVRSLL
210 220 230 240 250
EVVQTGAKNI EITVVKPDSD IVALSSEEIN QYVTQIEQEK QEQQEQDKKK

KSNH
Length:254
Mass (Da):28,439
Last modified:February 1, 1995 - v1
Checksum:i73AEE63B836E618A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34348 Genomic DNA Translation: AAA34903.1
Z74780 Genomic DNA Translation: CAA99040.1
BK006948 Genomic DNA Translation: DAA10745.1
PIRiB55904
RefSeqiNP_014604.1, NM_001183292.1

Genome annotation databases

EnsemblFungiiYOL038W; YOL038W; YOL038W
GeneIDi854119
KEGGisce:YOL038W

Similar proteinsi

Entry informationi

Entry nameiPSA4_YEAST
AccessioniPrimary (citable) accession number: P40303
Secondary accession number(s): D6W229
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 194 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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