UniProtKB - P40261 (NNMT_HUMAN)
Protein
Nicotinamide N-methyltransferase
Gene
NNMT
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Catalytic activityi
- EC:2.1.1.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 20 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 25 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 69 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 85 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 90 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 163 | S-adenosyl-L-methionine1 Publication | 1 |
GO - Molecular functioni
- nicotinamide N-methyltransferase activity Source: GO_Central
- N-methyltransferase activity Source: GO_Central
- pyridine N-methyltransferase activity Source: Reactome
GO - Biological processi
- animal organ regeneration Source: Ensembl
- methylation Source: Reactome
- NAD biosynthesis via nicotinamide riboside salvage pathway Source: Reactome
- response to drug Source: Ensembl
- response to organonitrogen compound Source: Ensembl
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.1, 2681 |
PathwayCommonsi | P40261 |
Reactomei | R-HSA-156581, Methylation R-HSA-197264, Nicotinamide salvaging R-HSA-2408508, Metabolism of ingested SeMet, Sec, MeSec into H2Se |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NNMT |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000166741.7 |
HGNCi | HGNC:7861, NNMT |
MIMi | 600008, gene |
neXtProti | NX_P40261 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 4837 |
OpenTargetsi | ENSG00000166741 |
PharmGKBi | PA251 |
Miscellaneous databases
Pharosi | P40261, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2346486 |
DrugBanki | DB00627, Niacin |
DrugCentrali | P40261 |
Polymorphism and mutation databases
BioMutai | NNMT |
DMDMi | 730163 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159706 | 1 – 264 | Nicotinamide N-methyltransferaseAdd BLAST | 264 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 39 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | P40261 |
jPOSTi | P40261 |
MassIVEi | P40261 |
PaxDbi | P40261 |
PeptideAtlasi | P40261 |
PRIDEi | P40261 |
ProteomicsDBi | 55358 |
TopDownProteomicsi | P40261 |
PTM databases
iPTMneti | P40261 |
MetOSitei | P40261 |
PhosphoSitePlusi | P40261 |
Expressioni
Tissue specificityi
Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
Gene expression databases
Bgeei | ENSG00000166741, Expressed in right lobe of liver and 210 other tissues |
ExpressionAtlasi | P40261, baseline and differential |
Genevisiblei | P40261, HS |
Organism-specific databases
HPAi | ENSG00000166741, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 110900, 7 interactors |
IntActi | P40261, 2 interactors |
STRINGi | 9606.ENSP00000441434 |
Chemistry databases
BindingDBi | P40261 |
Miscellaneous databases
RNActi | P40261, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40261 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40261 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 64 | S-adenosyl-L-methionine binding1 Publication | 2 | |
Regioni | 142 – 143 | S-adenosyl-L-methionine binding1 Publication | 2 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.Curated
Phylogenomic databases
eggNOGi | KOG4564, Eukaryota |
GeneTreei | ENSGT00390000011708 |
HOGENOMi | CLU_082526_1_1_1 |
InParanoidi | P40261 |
OMAi | YNFGSRH |
OrthoDBi | 1054662at2759 |
PhylomeDBi | P40261 |
TreeFami | TF313114 |
Family and domain databases
InterProi | View protein in InterPro IPR025820, NNMT/PNMT/TEMT_CS IPR000940, NNMT_TEMT_trans IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR10867, PTHR10867, 1 hit |
Pfami | View protein in Pfam PF01234, NNMT_PNMT_TEMT, 1 hit |
PIRSFi | PIRSF000384, PNMTase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS01100, NNMT_PNMT_TEMT, 1 hit PS51681, SAM_MT_NNMT_PNMT_TEMT, 1 hit |
i Sequence
Sequence statusi: Complete.
P40261-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC
60 70 80 90 100
LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK
110 120 130 140 150
EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG
160 170 180 190 200
AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK
210 220 230 240 250
SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE
260
GLFSLVARKL SRPL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08021 mRNA Translation: AAA19904.1 U20971, U20970 Genomic DNA Translation: AAA93158.1 BC000234 mRNA Translation: AAH00234.1 |
CCDSi | CCDS8368.1 |
PIRi | A54060 |
RefSeqi | NP_006160.1, NM_006169.2 |
Genome annotation databases
Ensembli | ENST00000299964; ENSP00000299964; ENSG00000166741 ENST00000535401; ENSP00000441434; ENSG00000166741 |
GeneIDi | 4837 |
KEGGi | hsa:4837 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U08021 mRNA Translation: AAA19904.1 U20971, U20970 Genomic DNA Translation: AAA93158.1 BC000234 mRNA Translation: AAH00234.1 |
CCDSi | CCDS8368.1 |
PIRi | A54060 |
RefSeqi | NP_006160.1, NM_006169.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2IIP | X-ray | 2.05 | A/B/C/D | 1-264 | [»] | |
3ROD | X-ray | 2.72 | A/B/C/D | 1-264 | [»] | |
5YJF | X-ray | 2.49 | A/B/C/D | 1-264 | [»] | |
6B1A | X-ray | 2.30 | A/B/C/D | 1-264 | [»] | |
6CHH | X-ray | 2.30 | A/B/C/D | 1-264 | [»] | |
6ORR | X-ray | 2.25 | A/B/C/D | 1-264 | [»] | |
6PVE | X-ray | 2.30 | A/B/C/D | 1-264 | [»] | |
6PVS | X-ray | 2.58 | A/B/C/D | 1-264 | [»] | |
SMRi | P40261 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110900, 7 interactors |
IntActi | P40261, 2 interactors |
STRINGi | 9606.ENSP00000441434 |
Chemistry databases
BindingDBi | P40261 |
ChEMBLi | CHEMBL2346486 |
DrugBanki | DB00627, Niacin |
DrugCentrali | P40261 |
PTM databases
iPTMneti | P40261 |
MetOSitei | P40261 |
PhosphoSitePlusi | P40261 |
Polymorphism and mutation databases
BioMutai | NNMT |
DMDMi | 730163 |
Proteomic databases
EPDi | P40261 |
jPOSTi | P40261 |
MassIVEi | P40261 |
PaxDbi | P40261 |
PeptideAtlasi | P40261 |
PRIDEi | P40261 |
ProteomicsDBi | 55358 |
TopDownProteomicsi | P40261 |
Protocols and materials databases
Antibodypediai | 18379, 462 antibodies |
DNASUi | 4837 |
Genome annotation databases
Ensembli | ENST00000299964; ENSP00000299964; ENSG00000166741 ENST00000535401; ENSP00000441434; ENSG00000166741 |
GeneIDi | 4837 |
KEGGi | hsa:4837 |
Organism-specific databases
CTDi | 4837 |
DisGeNETi | 4837 |
EuPathDBi | HostDB:ENSG00000166741.7 |
GeneCardsi | NNMT |
HGNCi | HGNC:7861, NNMT |
HPAi | ENSG00000166741, Tissue enhanced (liver) |
MIMi | 600008, gene |
neXtProti | NX_P40261 |
OpenTargetsi | ENSG00000166741 |
PharmGKBi | PA251 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4564, Eukaryota |
GeneTreei | ENSGT00390000011708 |
HOGENOMi | CLU_082526_1_1_1 |
InParanoidi | P40261 |
OMAi | YNFGSRH |
OrthoDBi | 1054662at2759 |
PhylomeDBi | P40261 |
TreeFami | TF313114 |
Enzyme and pathway databases
BRENDAi | 2.1.1.1, 2681 |
PathwayCommonsi | P40261 |
Reactomei | R-HSA-156581, Methylation R-HSA-197264, Nicotinamide salvaging R-HSA-2408508, Metabolism of ingested SeMet, Sec, MeSec into H2Se |
Miscellaneous databases
BioGRID-ORCSi | 4837, 4 hits in 839 CRISPR screens |
ChiTaRSi | NNMT, human |
EvolutionaryTracei | P40261 |
GeneWikii | NNMT |
GenomeRNAii | 4837 |
Pharosi | P40261, Tchem |
PROi | PR:P40261 |
RNActi | P40261, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166741, Expressed in right lobe of liver and 210 other tissues |
ExpressionAtlasi | P40261, baseline and differential |
Genevisiblei | P40261, HS |
Family and domain databases
InterProi | View protein in InterPro IPR025820, NNMT/PNMT/TEMT_CS IPR000940, NNMT_TEMT_trans IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR10867, PTHR10867, 1 hit |
Pfami | View protein in Pfam PF01234, NNMT_PNMT_TEMT, 1 hit |
PIRSFi | PIRSF000384, PNMTase, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS01100, NNMT_PNMT_TEMT, 1 hit PS51681, SAM_MT_NNMT_PNMT_TEMT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NNMT_HUMAN | |
Accessioni | P40261Primary (citable) accession number: P40261 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM