UniProtKB - P40260 (MEP1_YEAST)
Protein
Ammonium transporter MEP1
Gene
MEP1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. Can also transport methylamine. The affinity of MEP1 is about twenty times lower than that of MEP2. MEP3 has the lowest affinity.2 Publications
GO - Molecular functioni
- ammonium transmembrane transporter activity Source: SGD
GO - Biological processi
- ammonium transport Source: SGD
- nitrogen utilization Source: SGD
Keywordsi
Biological process | Ammonia transport, Transport |
Protein family/group databases
TCDBi | 1.A.11.3.1, the ammonium transporter channel (amt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ammonium transporter MEP1 |
Gene namesi | Name:MEP1 Synonyms:AMT1 Ordered Locus Names:YGR121C ORF Names:G6331 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003353, MEP1 |
VEuPathDBi | FungiDB:YGR121C |
Subcellular locationi
Other locations
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 18 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 19 – 39 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 40 – 49 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 50 – 70 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 71 – 109 | ExtracellularSequence analysisAdd BLAST | 39 | |
Transmembranei | 110 – 130 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 131 – 140 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 141 – 161 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 162 – 174 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 175 – 195 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 196 – 210 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 211 – 231 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 232 – 240 | ExtracellularSequence analysis | 9 | |
Transmembranei | 241 – 261 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 262 – 268 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 269 – 289 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 290 | ExtracellularSequence analysis | 1 | |
Transmembranei | 291 – 311 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 312 – 331 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 332 – 352 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 353 – 373 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 374 – 394 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 395 – 492 | CytoplasmicSequence analysisAdd BLAST | 98 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139754 | 1 – 492 | Ammonium transporter MEP1Add BLAST | 492 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 442 | PhosphoserineCombined sources | 1 | |
Modified residuei | 445 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P40260 |
PRIDEi | P40260 |
PTM databases
iPTMneti | P40260 |
Interactioni
Binary interactionsi
P40260
With | #Exp. | IntAct |
---|---|---|
MEP3 [P53390] | 3 | EBI-10714,EBI-10729 |
Protein-protein interaction databases
BioGRIDi | 33367, 132 interactors |
DIPi | DIP-5530N |
IntActi | P40260, 14 interactors |
MINTi | P40260 |
STRINGi | 4932.YGR121C |
Miscellaneous databases
RNActi | P40260, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0682, Eukaryota |
GeneTreei | ENSGT00530000064546 |
HOGENOMi | CLU_000445_33_0_1 |
InParanoidi | P40260 |
OMAi | CFILWIG |
Family and domain databases
Gene3Di | 1.10.3430.10, 1 hit |
InterProi | View protein in InterPro IPR029020, Ammonium/urea_transptr IPR001905, Ammonium_transpt IPR018047, Ammonium_transpt_CS IPR024041, NH4_transpt_AmtB-like_dom |
Pfami | View protein in Pfam PF00909, Ammonium_transp, 1 hit |
TIGRFAMsi | TIGR00836, amt, 1 hit |
PROSITEi | View protein in PROSITE PS01219, AMMONIUM_TRANSP, 1 hit |
i Sequence
Sequence statusi: Complete.
P40260-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL
60 70 80 90 100
ALIWVVLMAT LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK
110 120 130 140 150
KFDEDAYPEL AYATFQMMFS CVNLSIIAGA TAERGRLLPH MVFLFILATI
160 170 180 190 200
GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI EILSAVSGFV YSWFLGKRNE
210 220 230 240 250
KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS VYAFMNTCLS
260 270 280 290 300
AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ
310 320 330 340 350
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI
360 370 380 390 400
GMDGTTEHEG GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG
410 420 430 440 450
MRLRISEEAE EAGMDEDQIG EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR
460 470 480 490
VNNAHLAAER SSSGTNSSSD GNGEMIQSEK ILPIHQEDPA NR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77608 Genomic DNA Translation: CAA54699.1 X83099 Genomic DNA Translation: CAA58156.1 Z72906 Genomic DNA Translation: CAA97132.1 BK006941 Genomic DNA Translation: DAA08213.1 |
PIRi | S46225 |
RefSeqi | NP_011636.3, NM_001181250.3 |
Genome annotation databases
EnsemblFungii | YGR121C_mRNA; YGR121C; YGR121C |
GeneIDi | 853019 |
KEGGi | sce:YGR121C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77608 Genomic DNA Translation: CAA54699.1 X83099 Genomic DNA Translation: CAA58156.1 Z72906 Genomic DNA Translation: CAA97132.1 BK006941 Genomic DNA Translation: DAA08213.1 |
PIRi | S46225 |
RefSeqi | NP_011636.3, NM_001181250.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 33367, 132 interactors |
DIPi | DIP-5530N |
IntActi | P40260, 14 interactors |
MINTi | P40260 |
STRINGi | 4932.YGR121C |
Protein family/group databases
TCDBi | 1.A.11.3.1, the ammonium transporter channel (amt) family |
PTM databases
iPTMneti | P40260 |
Proteomic databases
PaxDbi | P40260 |
PRIDEi | P40260 |
Genome annotation databases
EnsemblFungii | YGR121C_mRNA; YGR121C; YGR121C |
GeneIDi | 853019 |
KEGGi | sce:YGR121C |
Organism-specific databases
SGDi | S000003353, MEP1 |
VEuPathDBi | FungiDB:YGR121C |
Phylogenomic databases
eggNOGi | KOG0682, Eukaryota |
GeneTreei | ENSGT00530000064546 |
HOGENOMi | CLU_000445_33_0_1 |
InParanoidi | P40260 |
OMAi | CFILWIG |
Miscellaneous databases
PROi | PR:P40260 |
RNActi | P40260, protein |
Family and domain databases
Gene3Di | 1.10.3430.10, 1 hit |
InterProi | View protein in InterPro IPR029020, Ammonium/urea_transptr IPR001905, Ammonium_transpt IPR018047, Ammonium_transpt_CS IPR024041, NH4_transpt_AmtB-like_dom |
Pfami | View protein in Pfam PF00909, Ammonium_transp, 1 hit |
TIGRFAMsi | TIGR00836, amt, 1 hit |
PROSITEi | View protein in PROSITE PS01219, AMMONIUM_TRANSP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MEP1_YEAST | |
Accessioni | P40260Primary (citable) accession number: P40260 Secondary accession number(s): D6VUQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families