Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thrombopoietin

Gene

Thpo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Hormone

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombopoietin
Alternative name(s):
C-mpl ligand
Short name:
ML
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
Short name:
MGDF
Myeloproliferative leukemia virus oncogene ligand
Gene namesi
Name:Thpo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:101875 Thpo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000841222 – 356ThrombopoietinAdd BLAST335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 172Sequence analysis
Disulfide bondi50 ↔ 106Sequence analysis
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi235N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi336N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi351N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP40226
PRIDEiP40226

PTM databases

PhosphoSitePlusiP40226

Expressioni

Tissue specificityi

Found mainly in the liver, kidney and skeletal muscle.

Inductioni

Expression in the liver and bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle).1 Publication

Gene expression databases

BgeeiENSMUSG00000022847 Expressed in 47 organ(s), highest expression level in kidney blood vessel
CleanExiMM_THPO
ExpressionAtlasiP40226 baseline and differential
GenevisibleiP40226 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075756

Structurei

3D structure databases

ProteinModelPortaliP40226
SMRiP40226
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

Sequence similaritiesi

Belongs to the EPO/TPO family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IVHV Eukaryota
ENOG41116B9 LUCA
GeneTreeiENSGT00390000006294
HOGENOMiHOG000082495
HOVERGENiHBG002468
InParanoidiP40226
KOiK06854
OMAiPLNGTHG
OrthoDBiEOG091G0DW3
PhylomeDBiP40226
TreeFamiTF338084

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR019767 EPO/TPO_CS
IPR001323 EPO_TPO
IPR003978 Thrombopoeitin
PANTHERiPTHR10560 PTHR10560, 1 hit
PfamiView protein in Pfam
PF00758 EPO_TPO, 1 hit
PRINTSiPR01485 THROMBOPTN
SUPFAMiSSF47266 SSF47266, 1 hit
PROSITEiView protein in PROSITE
PS00817 EPO_TPO, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P40226-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELTDLLLAA MLLAVARLTL SSPVAPACDP RLLNKLLRDS HLLHSRLSQC
60 70 80 90 100
PDVDPLSIPV LLPAVDFSLG EWKTQTEQSK AQDILGAVSL LLEGVMAARG
110 120 130 140 150
QLEPSCLSSL LGQLSGQVRL LLGALQGLLG TQLPLQGRTT AHKDPNALFL
160 170 180 190 200
SLQQLLRGKV RFLLLVEGPT LCVRRTLPTT AVPSSTSQLL TLNKFPNRTS
210 220 230 240 250
GLLETNFSVT ARTAGPGLLS RLQGFRVKIT PGQLNQTSRS PVQISGYLNR
260 270 280 290 300
THGPVNGTHG LFAGTSLQTL EASDISPGAF NKGSLAFNLQ GGLPPSPSLA
310 320 330 340 350
PDGHTPFPPS PALPTTHGSP PQLHPLFPDP STTMPNSTAP HPVTMYPHPR

NLSQET
Length:356
Mass (Da):37,836
Last modified:February 1, 1995 - v1
Checksum:iD346DBAE8392053C
GO
Isoform 2 (identifier: P40226-2) [UniParc]FASTAAdd to basket
Also known as: TPO-2

The sequence of this isoform differs from the canonical sequence as follows:
     133-136: Missing.

Show »
Length:352
Mass (Da):37,384
Checksum:iB7121C4BC7CCAF4A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A338P6C8A0A338P6C8_MOUSE
Thrombopoietin
Thpo
231Annotation score:
A0A338P6S5A0A338P6S5_MOUSE
Thrombopoietin
Thpo
385Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001452133 – 136Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34169 mRNA Translation: AAA40436.1
BC003803 mRNA Translation: AAH03803.1
CCDSiCCDS37292.1 [P40226-1]
PIRiS45330
RefSeqiNP_001166976.1, NM_001173505.1 [P40226-1]
NP_001276823.1, NM_001289894.1 [P40226-2]
NP_033405.1, NM_009379.3 [P40226-1]
UniGeneiMm.3943

Genome annotation databases

EnsembliENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847 [P40226-1]
ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847 [P40226-1]
GeneIDi21832
KEGGimmu:21832
UCSCiuc007yra.2 mouse [P40226-1]
uc033gyk.1 mouse [P40226-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34169 mRNA Translation: AAA40436.1
BC003803 mRNA Translation: AAH03803.1
CCDSiCCDS37292.1 [P40226-1]
PIRiS45330
RefSeqiNP_001166976.1, NM_001173505.1 [P40226-1]
NP_001276823.1, NM_001289894.1 [P40226-2]
NP_033405.1, NM_009379.3 [P40226-1]
UniGeneiMm.3943

3D structure databases

ProteinModelPortaliP40226
SMRiP40226
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075756

PTM databases

PhosphoSitePlusiP40226

Proteomic databases

PaxDbiP40226
PRIDEiP40226

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076422; ENSMUSP00000075756; ENSMUSG00000022847 [P40226-1]
ENSMUST00000115437; ENSMUSP00000111097; ENSMUSG00000022847 [P40226-1]
GeneIDi21832
KEGGimmu:21832
UCSCiuc007yra.2 mouse [P40226-1]
uc033gyk.1 mouse [P40226-2]

Organism-specific databases

CTDi7066
MGIiMGI:101875 Thpo

Phylogenomic databases

eggNOGiENOG410IVHV Eukaryota
ENOG41116B9 LUCA
GeneTreeiENSGT00390000006294
HOGENOMiHOG000082495
HOVERGENiHBG002468
InParanoidiP40226
KOiK06854
OMAiPLNGTHG
OrthoDBiEOG091G0DW3
PhylomeDBiP40226
TreeFamiTF338084

Miscellaneous databases

PROiPR:P40226
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022847 Expressed in 47 organ(s), highest expression level in kidney blood vessel
CleanExiMM_THPO
ExpressionAtlasiP40226 baseline and differential
GenevisibleiP40226 MM

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR019767 EPO/TPO_CS
IPR001323 EPO_TPO
IPR003978 Thrombopoeitin
PANTHERiPTHR10560 PTHR10560, 1 hit
PfamiView protein in Pfam
PF00758 EPO_TPO, 1 hit
PRINTSiPR01485 THROMBOPTN
SUPFAMiSSF47266 SSF47266, 1 hit
PROSITEiView protein in PROSITE
PS00817 EPO_TPO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPO_MOUSE
AccessioniPrimary (citable) accession number: P40226
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again