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UniProtKB - P40160 (RIO2_YEAST)
Protein
Serine/threonine-protein kinase RIO2
Gene
RIO2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for the final endonucleolytic cleavage of 20S pre-rRNA at site D in the cytoplasm, converting it into the mature 18S rRNA. Involved in normal export of the pre-40S particles from the nucleus to the cytoplasm. No longer associates with pre-40S subunits in RPS19 disruptions, suggesting it acts after the ribosomal protein in 18S rRNA maturation.
5 PublicationsMiscellaneous
Present with 10300 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Cofactori
Mg2+1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 123 | ATPBy similarity | 1 | |
Active sitei | 229 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
GO - Biological processi
- maturation of SSU-rRNA Source: GO_Central
- maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- positive regulation of RNA import into nucleus Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Ribosome biogenesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RIO2 Ordered Locus Names:YNL207W ORF Names:N1342 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005151, RIO2 |
VEuPathDBi | FungiDB:YNL207W |
Subcellular locationi
Cytosol
- cytosol Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
- preribosome, small subunit precursor Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | Y → H in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with V-186, H-210, D-364, G-371 and G-417. 1 Publication | 1 | |
Mutagenesisi | 105 | K → I: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 186 | I → V in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, H-210, D-364, G-371 and G-417. 1 Publication | 1 | |
Mutagenesisi | 210 | Y → H in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, D-364, G-371 and G-417. 1 Publication | 1 | |
Mutagenesisi | 227 | H → F: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 229 | D → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 364 | E → D in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, G-371 and G-417. 1 Publication | 1 | |
Mutagenesisi | 371 | D → G in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-417. 1 Publication | 1 | |
Mutagenesisi | 417 | D → G in RIO2-1; nuclear export of ribosomal 40S subunits impaired; when associated with H-73, V-186, H-210, D-364 and G-371. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213532 | 1 – 425 | Serine/threonine-protein kinase RIO2Add BLAST | 425 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 346 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Autophosphorylated.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P40160 |
PaxDbi | P40160 |
PRIDEi | P40160 |
PTM databases
iPTMneti | P40160 |
Interactioni
Subunit structurei
Component of a late pre-40S ribosomal particle, composed of the 40S ribosomal proteins and the non-ribosomal factors DIM1, DIM2, ENP1, HRR25, LTV1, NOB1, RIO2 and TSR1.
1 PublicationBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 35629, 225 interactors |
DIPi | DIP-4894N |
IntActi | P40160, 50 interactors |
MINTi | P40160 |
STRINGi | 4932.YNL207W |
Miscellaneous databases
RNActi | P40160, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P40160 |
SMRi | P40160 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 95 – 257 | Protein kinaseAdd BLAST | 163 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 350 – 420 | DisorderedSequence analysisAdd BLAST | 71 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 357 – 400 | Acidic residuesSequence analysisAdd BLAST | 44 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2268, Eukaryota |
GeneTreei | ENSGT00390000003255 |
HOGENOMi | CLU_018693_0_0_1 |
InParanoidi | P40160 |
OMAi | GYTNFRE |
Family and domain databases
CDDi | cd05144, RIO2_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR030484, Rio2 IPR015285, RIO2_wHTH_N IPR000687, RIO_kinase IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF09202, Rio2_N, 1 hit |
SMARTi | View protein in SMART SM00090, RIO, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF56112, SSF56112, 1 hit |
i Sequence
Sequence statusi: Complete.
P40160-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLDTSHMRY LTTDDFRVLQ AVEQGSRSHE VVPTPLIHQI SGMRSQSGTN
60 70 80 90 100
RAISDLAKLS LISKMRNVKY DGYRLTYNGI DYLALKTMLN RDTVYSVGNT
110 120 130 140 150
IGVGKESDIY KVSDKNGNPR VMKIHRLGRT SFHSVRNNRD YLKKSNQGAN
160 170 180 190 200
WMHLSRLAAN KEYQFMSMLY SKGFKVPEPF DNSRHIVVME LIEGYPMRRL
210 220 230 240 250
RKHKNIPKLY SDLMCFIVDL ANSGLIHCDF NEFNIMIKDK LEDENDCGFV
260 270 280 290 300
VIDFPQCISI QHQDADYYFQ RDVDCIRRFF KKKLKYEPKP DSSMLDTEGF
310 320 330 340 350
GDGYKYAYPD FKRDVKRTDN LDELVQASGF SKKHPGDRGL ETAVESMRNA
360 370 380 390 400
VYNSDDDMSN DEAEEENGEG DYSEEDEYYD SELDNESSED DSEDAQEEEN
410 420
ERIIEALSSG VENLKMDKLG NYILE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78898 Genomic DNA Translation: CAA55501.1 Z71483 Genomic DNA Translation: CAA96109.1 BK006947 Genomic DNA Translation: DAA10349.1 |
PIRi | S50724 |
RefSeqi | NP_014192.1, NM_001183045.1 |
Genome annotation databases
EnsemblFungii | YNL207W_mRNA; YNL207W; YNL207W |
GeneIDi | 855514 |
KEGGi | sce:YNL207W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X78898 Genomic DNA Translation: CAA55501.1 Z71483 Genomic DNA Translation: CAA96109.1 BK006947 Genomic DNA Translation: DAA10349.1 |
PIRi | S50724 |
RefSeqi | NP_014192.1, NM_001183045.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EML | electron microscopy | 3.60 | r | 1-425 | [»] | |
6FAI | electron microscopy | 3.40 | l | 1-425 | [»] | |
6RBD | electron microscopy | 3.47 | l | 1-425 | [»] | |
6Y7C | electron microscopy | 3.80 | l | 1-425 | [»] | |
AlphaFoldDBi | P40160 | |||||
SMRi | P40160 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35629, 225 interactors |
DIPi | DIP-4894N |
IntActi | P40160, 50 interactors |
MINTi | P40160 |
STRINGi | 4932.YNL207W |
PTM databases
iPTMneti | P40160 |
Proteomic databases
MaxQBi | P40160 |
PaxDbi | P40160 |
PRIDEi | P40160 |
Genome annotation databases
EnsemblFungii | YNL207W_mRNA; YNL207W; YNL207W |
GeneIDi | 855514 |
KEGGi | sce:YNL207W |
Organism-specific databases
SGDi | S000005151, RIO2 |
VEuPathDBi | FungiDB:YNL207W |
Phylogenomic databases
eggNOGi | KOG2268, Eukaryota |
GeneTreei | ENSGT00390000003255 |
HOGENOMi | CLU_018693_0_0_1 |
InParanoidi | P40160 |
OMAi | GYTNFRE |
Enzyme and pathway databases
Reactomei | R-SCE-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
PROi | PR:P40160 |
RNActi | P40160, protein |
Family and domain databases
CDDi | cd05144, RIO2_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR030484, Rio2 IPR015285, RIO2_wHTH_N IPR000687, RIO_kinase IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF09202, Rio2_N, 1 hit |
SMARTi | View protein in SMART SM00090, RIO, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF56112, SSF56112, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RIO2_YEAST | |
Accessioni | P40160Primary (citable) accession number: P40160 Secondary accession number(s): D6W0Y3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families