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Protein

Calmodulin-sensitive adenylate cyclase

Gene

cya

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. EF is a calmodulin-dependent adenylyl cyclase that, when associated with PA, causes edema. EF is not toxic by itself and it is required for the survival of germinated spores within macrophages at the early stages of infection. Provokes dramatic elevation of intracellular cAMP levels in the host.

Miscellaneous

EF binds to the heptamer formed by cleaved PA on the host cell membrane. This step is followed by internalization of the hetero-oligomeric complex by receptor-mediated endocytosis. EF requires passage through an acidic vesicle for activity. At acidic pH, the pore is inserted into the membrane, allowing translocation of EF, which probably contributes actively to its own insertion into the membrane. EF remains associated to the vesicle membrane after translocation to the cytosol, with the catalytic domains being exposed on the cytoplasmic face.

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Activity regulationi

It has an absolute requirement for host calmodulin for its activation. Inhibited by ethyl 5-aminopyrazolo[1,5-a]quinazoline-3-carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei351Proton acceptor1
Metal bindingi491Magnesium1
Metal bindingi493Magnesium1

GO - Molecular functioni

  • adenylate cyclase activity Source: Reactome
  • ATP binding Source: CAFA
  • calcium- and calmodulin-responsive adenylate cyclase activity Source: CACAO
  • calmodulin binding Source: CAFA
  • metal ion binding Source: CAFA
  • metallopeptidase activity Source: InterPro
  • toxin activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Lyase, Toxin
Biological processcAMP biosynthesis, Virulence
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5210891 Uptake and function of anthrax toxins

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-sensitive adenylate cyclase (EC:4.6.1.1)
Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase
Anthrax edema toxin adenylate cyclase component
Edema factor
Short name:
EF
Gene namesi
Name:cya
Ordered Locus Names:pXO1-122, BXA0141, GBAA_pXO1_0142
Encoded oniPlasmid pXO10 Publication
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Plasmid pXO1

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi169V → A: No effect. 1 Publication1
Mutagenesisi170Y → A: Loss of cytotoxicity due to inability to bind PA. 1 Publication1
Mutagenesisi171Y → A: Loss of cytotoxicity due to inability to bind PA. 1 Publication1
Mutagenesisi172E → A: No effect. 1 Publication1
Mutagenesisi173I → A: Loss of cytotoxicity due to inability to bind PA. 1 Publication1
Mutagenesisi174G → A: No effect. 1 Publication1
Mutagenesisi175K → A: Loss of cytotoxicity due to inability to bind PA. 1 Publication1
Mutagenesisi329R → M: Great decrease in activity. 1 Publication1
Mutagenesisi346K → M or R: Loss of activity. 2 Publications1
Mutagenesisi346K → Q: Loss of activity due to inability to bind the substrate. 2 Publications1
Mutagenesisi353K → M, R or A: Loss of activity. 1 Publication1
Mutagenesisi436E → Q: Decreases activity. 1 Publication1
Mutagenesisi443E → Q: Decreases activity. 1 Publication1
Mutagenesisi491D → N: Great decrease in activity. 1 Publication1
Mutagenesisi493D → N: Great decrease in activity. 1 Publication1
Mutagenesisi523L → A: Little effect on activation by calmodulin. 1 Publication1
Mutagenesisi525K → A: Great decrease in calmodulin binding. 1 Publication1
Mutagenesisi526Q → A: Little effect on activation by calmodulin. 1 Publication1
Mutagenesisi529V → A: Little effect on activation by calmodulin. 1 Publication1
Mutagenesisi577H → N or D: Loss of function. 1 Publication1
Mutagenesisi583N → A: Decreases activity. 1 Publication1
Mutagenesisi583N → Q or H: Loss of function. 1 Publication1
Mutagenesisi588E → A: Loss of function. 1 Publication1
Mutagenesisi590D → A: Decreases activity. 1 Publication1
Mutagenesisi639N → A: Decreases catalysis rate. 1 Publication1
Mutagenesisi647D → A: Decreases activity due to reduced activation by calmodulin. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5396

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000000131734 – 800Calmodulin-sensitive adenylate cyclaseAdd BLAST767

Proteomic databases

PRIDEiP40136

Interactioni

Subunit structurei

Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these is toxic by itself. PA+LF forms the lethal toxin (LeTx); PA+EF forms the edema toxin (EdTx). EF probably forms oligomers as part of the translocation machinery, formed by a heterocomplex of PA63 monomers and EF subunits, and it is functional as a monomer in the host cell.

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-31055N
IntActiP40136, 3 interactors

Chemistry databases

BindingDBiP40136

Structurei

Secondary structure

1800
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00395
ProteinModelPortaliP40136
SMRiP40136
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40136

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 290Interaction with protective antigenAdd BLAST257
Regioni294 – 349Catalytic CA1Add BLAST56
Regioni350 – 489Catalytic CBAdd BLAST140
Regioni490 – 622Catalytic CA2Add BLAST133
Regioni623 – 800Interaction with calmodulinAdd BLAST178

Domaini

The N-terminal region contains the residues responsible for binding to PA63. The C-terminal region contains the calmodulin-dependent activation domain and the catalytic site. This region is composed of three globular domains: CA, CB and a helical domain connected to CA by a linker. The active site lies at the interface of CA and CB. The metal ion is coordinated by residues from CA; calmodulin probably binds in a multistep fashion first to residues in CA and then to residues present in the linker and the helical domain.
The PA-binding region is found in both B.anthracis EF and LF.

Sequence similaritiesi

Belongs to the adenylyl cyclase class-2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000034573
KOiK11029

Family and domain databases

Gene3Di3.40.390.10, 1 hit
3.90.1760.10, 1 hit
InterProiView protein in InterPro
IPR035099 Anthrax_toxin_C-terminal
IPR005165 Anthrax_toxin_edema_cen
IPR037017 Anthrax_toxin_edema_cen_sf
IPR003541 Anthrax_toxin_lethal/edema
IPR014781 Anthrax_toxin_lethal/edema_N/C
IPR024079 MetalloPept_cat_dom_sf
PfamiView protein in Pfam
PF03497 Anthrax_toxA, 1 hit
PF07737 ATLF, 1 hit
PRINTSiPR01392 ANTHRAXTOXNA
SUPFAMiSSF81298 SSF81298, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40136-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRNKFIPNK FSIISFSVLL FAISSSQAIE VNAMNEHYTE SDIKRNHKTE
60 70 80 90 100
KNKTEKEKFK DSINNLVKTE FTNETLDKIQ QTQDLLKKIP KDVLEIYSEL
110 120 130 140 150
GGEIYFTDID LVEHKELQDL SEEEKNSMNS RGEKVPFASR FVFEKKRETP
160 170 180 190 200
KLIINIKDYA INSEQSKEVY YEIGKGISLD IISKDKSLDP EFLNLIKSLS
210 220 230 240 250
DDSDSSDLLF SQKFKEKLEL NNKSIDINFI KENLTEFQHA FSLAFSYYFA
260 270 280 290 300
PDHRTVLELY APDMFEYMNK LEKGGFEKIS ESLKKEGVEK DRIDVLKGEK
310 320 330 340 350
ALKASGLVPE HADAFKKIAR ELNTYILFRP VNKLATNLIK SGVATKGLNV
360 370 380 390 400
HGKSSDWGPV AGYIPFDQDL SKKHGQQLAV EKGNLENKKS ITEHEGEIGK
410 420 430 440 450
IPLKLDHLRI EELKENGIIL KGKKEIDNGK KYYLLESNNQ VYEFRISDEN
460 470 480 490 500
NEVQYKTKEG KITVLGEKFN WRNIEVMAKN VEGVLKPLTA DYDLFALAPS
510 520 530 540 550
LTEIKKQIPQ KEWDKVVNTP NSLEKQKGVT NLLIKYGIER KPDSTKGTLS
560 570 580 590 600
NWQKQMLDRL NEAVKYTGYT GGDVVNHGTE QDNEEFPEKD NEIFIINPEG
610 620 630 640 650
EFILTKNWEM TGRFIEKNIT GKDYLYYFNR SYNKIAPGNK AYIEWTDPIT
660 670 680 690 700
KAKINTIPTS AEFIKNLSSI RRSSNVGVYK DSGDKDEFAK KESVKKIAGY
710 720 730 740 750
LSDYYNSANH IFSQEKKRKI SIFRGIQAYN EIENVLKSKQ IAPEYKNYFQ
760 770 780 790 800
YLKERITNQV QLLLTHQKSN IEFKLLYKQL NFTENETDNF EVFQKIIDEK
Length:800
Mass (Da):92,478
Last modified:February 1, 1995 - v1
Checksum:iF4F7EB485DF4C5A6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti350V → E in AAA79215 (PubMed:3149607).Curated1
Sequence conflicti510Q → T AA sequence (PubMed:3149607).Curated1
Sequence conflicti512 – 513EW → RM AA sequence (PubMed:3149607).Curated2
Sequence conflicti760V → L in CAA00652 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23179 Genomic DNA Translation: AAA22374.1
M24074 Genomic DNA Translation: AAA79215.1
A07289 Unassigned DNA Translation: CAA00652.1 Sequence problems.
AF065404 Genomic DNA Translation: AAD32426.1
AE011190 Genomic DNA Translation: AAM26089.1
AE017336 Genomic DNA Translation: AAT28883.2
AJ413930 Genomic DNA Translation: CAC93924.1
AJ413931 Genomic DNA Translation: CAC93925.1
PIRiB59106
JS0029
RefSeqiNP_052818.1, NC_001496.1
WP_000197748.1, NZ_QPKQ01000017.1

Genome annotation databases

EnsemblBacteriaiAAM26089; AAM26089; BX_A0142
AAT28883; AAT28883; GBAA_pXO1_0142
GeneIDi3361726
KEGGibar:GBAA_pXO1_0142

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23179 Genomic DNA Translation: AAA22374.1
M24074 Genomic DNA Translation: AAA79215.1
A07289 Unassigned DNA Translation: CAA00652.1 Sequence problems.
AF065404 Genomic DNA Translation: AAD32426.1
AE011190 Genomic DNA Translation: AAM26089.1
AE017336 Genomic DNA Translation: AAT28883.2
AJ413930 Genomic DNA Translation: CAC93924.1
AJ413931 Genomic DNA Translation: CAC93925.1
PIRiB59106
JS0029
RefSeqiNP_052818.1, NC_001496.1
WP_000197748.1, NZ_QPKQ01000017.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K8TX-ray2.60A291-800[»]
1K90X-ray2.75A/B/C291-800[»]
1K93X-ray2.95A/B/C291-800[»]
1LVCX-ray3.60A/B/C291-800[»]
1PK0X-ray3.30A/B/C292-798[»]
1S26X-ray3.00A/B/C291-800[»]
1SK6X-ray3.20A/B/C291-800[»]
1XFUX-ray3.35A/B/C/D/E/F64-800[»]
1XFVX-ray3.35A/B/C/D/E/F33-800[»]
1XFWX-ray3.40A/B/C/D/E/F33-800[»]
1XFXX-ray3.20A/B/C/D/E/F33-800[»]
1XFYX-ray3.30A/B/C/D/E/F33-800[»]
1XFZX-ray3.25A/B/C/D/E/F33-800[»]
1Y0VX-ray3.60A/B/C/D/E/F33-800[»]
DisProtiDP00395
ProteinModelPortaliP40136
SMRiP40136
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-31055N
IntActiP40136, 3 interactors

Chemistry databases

BindingDBiP40136
ChEMBLiCHEMBL5396

Proteomic databases

PRIDEiP40136

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM26089; AAM26089; BX_A0142
AAT28883; AAT28883; GBAA_pXO1_0142
GeneIDi3361726
KEGGibar:GBAA_pXO1_0142

Phylogenomic databases

HOGENOMiHOG000034573
KOiK11029

Enzyme and pathway databases

ReactomeiR-HSA-5210891 Uptake and function of anthrax toxins

Miscellaneous databases

EvolutionaryTraceiP40136
PROiPR:P40136

Family and domain databases

Gene3Di3.40.390.10, 1 hit
3.90.1760.10, 1 hit
InterProiView protein in InterPro
IPR035099 Anthrax_toxin_C-terminal
IPR005165 Anthrax_toxin_edema_cen
IPR037017 Anthrax_toxin_edema_cen_sf
IPR003541 Anthrax_toxin_lethal/edema
IPR014781 Anthrax_toxin_lethal/edema_N/C
IPR024079 MetalloPept_cat_dom_sf
PfamiView protein in Pfam
PF03497 Anthrax_toxA, 1 hit
PF07737 ATLF, 1 hit
PRINTSiPR01392 ANTHRAXTOXNA
SUPFAMiSSF81298 SSF81298, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYAA_BACAN
AccessioniPrimary (citable) accession number: P40136
Secondary accession number(s): Q937W4, Q937W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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