UniProtKB - P40056 (GET2_YEAST)
Protein
Golgi to ER traffic protein 2
Gene
GET2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in DNA replication and DNA damage response and also in cell wall function.UniRule annotation7 Publications
GO - Molecular functioni
- protein-membrane adaptor activity Source: SGD
GO - Biological processi
- mitophagy Source: SGD
- protein insertion into ER membrane Source: SGD
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: SGD
Keywordsi
Biological process | ER-Golgi transport, Transport |
Protein family/group databases
TCDBi | 3.A.21.1.1, the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family |
Names & Taxonomyi
Protein namesi | Recommended name: Golgi to ER traffic protein 2UniRule annotationAlternative name(s): Guided entry of tail-anchored proteins 2 Hydroxyurea resistance protein 2UniRule annotation Required for meiotic nuclear division protein 7UniRule annotation |
Gene namesi | Ordered Locus Names:YER083C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YER083C |
SGDi | S000000885, GET2 |
Subcellular locationi
Endoplasmic reticulum
Golgi apparatus
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
- GET complex Source: SGD
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 148 | CytoplasmicUniRule annotationAdd BLAST | 147 | |
Transmembranei | 149 – 169 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 170 – 196 | LumenalUniRule annotationAdd BLAST | 27 | |
Transmembranei | 197 – 216 | HelicalUniRule annotationAdd BLAST | 20 | |
Topological domaini | 217 – 263 | CytoplasmicUniRule annotationAdd BLAST | 47 | |
Transmembranei | 264 – 284 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 285 | LumenalUniRule annotation | 1 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000097362 | 2 – 285 | Golgi to ER traffic protein 2Add BLAST | 284 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 45 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 173 | N-linked (GlcNAc...) asparagineUniRule annotation | 1 | |
Glycosylationi | 196 | N-linked (GlcNAc...) asparagineUniRule annotation | 1 |
Keywords - PTMi
Acetylation, Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P40056 |
PaxDbi | P40056 |
PRIDEi | P40056 |
TopDownProteomicsi | P40056 |
PTM databases
iPTMneti | P40056 |
Interactioni
Subunit structurei
Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.
UniRule annotation4 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 36828, 970 interactors |
ComplexPortali | CPX-956, GET complex |
DIPi | DIP-4508N |
IntActi | P40056, 10 interactors |
MINTi | P40056 |
STRINGi | 4932.YER083C |
Miscellaneous databases
RNActi | P40056, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40056 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P40056 |
Family & Domainsi
Sequence similaritiesi
Belongs to the GET2 family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QW0H, Eukaryota |
HOGENOMi | CLU_066477_0_0_1 |
InParanoidi | P40056 |
OMAi | GAMVLPQ |
Family and domain databases
HAMAPi | MF_03114, Get2, 1 hit |
InterProi | View protein in InterPro IPR014802, GET2 IPR028143, Get2/sif1 |
PANTHERi | PTHR28263, PTHR28263, 1 hit |
Pfami | View protein in Pfam PF08690, GET2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P40056-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSELTEAEKR RLLRERRQKK FSNGGASSRL NKITGQASSH LNAESPLDAP
60 70 80 90 100
SAAKTTPPAS VHSATPDIKE DSNVAPQLDL LKQLAAMQGQ GTGKSTPQDS
110 120 130 140 150
STPDLLSLLS SMNTGMPSAE GTPSFGQAAP AAPINQAALD YHDYLLNRLK
160 170 180 190 200
AWTILVKWVF FLLPYLYLIT RPNSSVWPAY AFTQSAWFAP LRNPSNFTRI
210 220 230 240 250
FATFEFLSIS IYYQLLKNVE HKSKIKNLQD TNKLVKLVSL VPEGVIPVAN
260 270 280
LKGKLITLLQ YWDLLSMLIT DISFVLIVLG LLTYL
Sequence cautioni
The sequence AAB64638 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18839 Genomic DNA Translation: AAB64638.1 Different initiation. BK006939 Genomic DNA Translation: DAA07744.1 |
PIRi | S50586 |
RefSeqi | NP_011006.2, NM_001178974.1 |
Genome annotation databases
EnsemblFungii | YER083C_mRNA; YER083C; YER083C |
GeneIDi | 856817 |
KEGGi | sce:YER083C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18839 Genomic DNA Translation: AAB64638.1 Different initiation. BK006939 Genomic DNA Translation: DAA07744.1 |
PIRi | S50586 |
RefSeqi | NP_011006.2, NM_001178974.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SJD | X-ray | 4.60 | D/E | 2-35 | [»] | |
3ZS9 | X-ray | 2.10 | C/D | 1-38 | [»] | |
SMRi | P40056 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36828, 970 interactors |
ComplexPortali | CPX-956, GET complex |
DIPi | DIP-4508N |
IntActi | P40056, 10 interactors |
MINTi | P40056 |
STRINGi | 4932.YER083C |
Protein family/group databases
TCDBi | 3.A.21.1.1, the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family |
PTM databases
iPTMneti | P40056 |
Proteomic databases
MaxQBi | P40056 |
PaxDbi | P40056 |
PRIDEi | P40056 |
TopDownProteomicsi | P40056 |
Genome annotation databases
EnsemblFungii | YER083C_mRNA; YER083C; YER083C |
GeneIDi | 856817 |
KEGGi | sce:YER083C |
Organism-specific databases
EuPathDBi | FungiDB:YER083C |
SGDi | S000000885, GET2 |
Phylogenomic databases
eggNOGi | ENOG502QW0H, Eukaryota |
HOGENOMi | CLU_066477_0_0_1 |
InParanoidi | P40056 |
OMAi | GAMVLPQ |
Miscellaneous databases
EvolutionaryTracei | P40056 |
PROi | PR:P40056 |
RNActi | P40056, protein |
Family and domain databases
HAMAPi | MF_03114, Get2, 1 hit |
InterProi | View protein in InterPro IPR014802, GET2 IPR028143, Get2/sif1 |
PANTHERi | PTHR28263, PTHR28263, 1 hit |
Pfami | View protein in Pfam PF08690, GET2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GET2_YEAST | |
Accessioni | P40056Primary (citable) accession number: P40056 Secondary accession number(s): D3DLZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | May 16, 2006 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD