UniProtKB - P40046 (VTC1_YEAST)
Protein
Vacuolar transporter chaperone 1
Gene
VTC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the vacuolar transporter chaperone (VTC) complex, which plays a role in vacuolar membrane fusion. Required for SEC18/NSF activity in SNARE priming, membrane binding of LMA1 and V0 trans-complex formation.3 Publications
Miscellaneous
Present with 12074 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- mRNA binding Source: SGD
GO - Biological processi
- lysosomal microautophagy Source: SGD
- polyphosphate biosynthetic process Source: SGD
- polyphosphate metabolic process Source: SGD
- vacuolar transport Source: SGD
- vacuole fusion, non-autophagic Source: SGD
Keywordsi
Molecular function | Chaperone |
Protein family/group databases
TCDBi | 9.B.51.1.6, the uncharacterized duf202/yidh (yidh) family |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar transporter chaperone 1Alternative name(s): Negative regulator of CDC42 protein 1 Phosphate metabolism protein 4 |
Gene namesi | Name:VTC1 Synonyms:NRF1, PHM4 Ordered Locus Names:YER072W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000874, VTC1 |
VEuPathDBi | FungiDB:YER072W |
Subcellular locationi
Vacuole
- Vacuole membrane 2 Publications; Multi-pass membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Nucleus
- nuclear membrane Source: SGD
Vacuole
- fungal-type vacuole membrane Source: SGD
- intrinsic component of vacuolar membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- vacuolar transporter chaperone complex Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 32 | Cytoplasmic1 PublicationAdd BLAST | 31 | |
Transmembranei | 33 – 53 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 54 – 59 | Vacuolar1 Publication | 6 | |
Transmembranei | 60 – 80 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 81 – 98 | Cytoplasmic1 PublicationAdd BLAST | 18 | |
Transmembranei | 99 – 119 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 120 – 129 | Vacuolar1 Publication | 10 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000065934 | 2 – 129 | Vacuolar transporter chaperone 1Add BLAST | 128 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P40046 |
PaxDbi | P40046 |
PRIDEi | P40046 |
TopDownProteomicsi | P40046 |
PTM databases
iPTMneti | P40046 |
Expressioni
Inductioni
By low phosphate.1 Publication
Interactioni
Subunit structurei
The VTC complex is an integral membrane heterooligomer composed of VTC1, VTC2, VTC3 and VTC4. The complex interacts with the v-SNARE NYV1 and with the V0 subunit of V-ATPase VPH1.
1 PublicationProtein-protein interaction databases
BioGRIDi | 36815, 148 interactors |
ComplexPortali | CPX-784, Vacuolar transporter chaperone complex |
IntActi | P40046, 16 interactors |
MINTi | P40046 |
STRINGi | 4932.YER072W |
Miscellaneous databases
RNActi | P40046, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the VTC1 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4580, Eukaryota |
GeneTreei | ENSGT00940000176488 |
HOGENOMi | CLU_141247_1_0_1 |
InParanoidi | P40046 |
OMAi | TYHWRAS |
Family and domain databases
InterProi | View protein in InterPro IPR003807, DUF202 |
Pfami | View protein in Pfam PF02656, DUF202, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P40046-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSAPLLQRT PGKKIALPTR VEPKVFFANE RTFLSWLNFT VMLGGLGVGL
60 70 80 90 100
LNFGDKIGRV SAGLFTFVAM GTMIYALVTY HWRAAAIRRR GSGPYDDRLG
110 120
PTLLCFFLLV AVIINFILRL KYNDANTKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18813 Genomic DNA Translation: AAB64608.1 BK006939 Genomic DNA Translation: DAA07731.1 |
PIRi | S50575 |
RefSeqi | NP_010995.1, NM_001178963.1 |
Genome annotation databases
EnsemblFungii | YER072W_mRNA; YER072W; YER072W |
GeneIDi | 856803 |
KEGGi | sce:YER072W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18813 Genomic DNA Translation: AAB64608.1 BK006939 Genomic DNA Translation: DAA07731.1 |
PIRi | S50575 |
RefSeqi | NP_010995.1, NM_001178963.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36815, 148 interactors |
ComplexPortali | CPX-784, Vacuolar transporter chaperone complex |
IntActi | P40046, 16 interactors |
MINTi | P40046 |
STRINGi | 4932.YER072W |
Protein family/group databases
TCDBi | 9.B.51.1.6, the uncharacterized duf202/yidh (yidh) family |
PTM databases
iPTMneti | P40046 |
Proteomic databases
MaxQBi | P40046 |
PaxDbi | P40046 |
PRIDEi | P40046 |
TopDownProteomicsi | P40046 |
Protocols and materials databases
DNASUi | 856803 |
Genome annotation databases
EnsemblFungii | YER072W_mRNA; YER072W; YER072W |
GeneIDi | 856803 |
KEGGi | sce:YER072W |
Organism-specific databases
SGDi | S000000874, VTC1 |
VEuPathDBi | FungiDB:YER072W |
Phylogenomic databases
eggNOGi | KOG4580, Eukaryota |
GeneTreei | ENSGT00940000176488 |
HOGENOMi | CLU_141247_1_0_1 |
InParanoidi | P40046 |
OMAi | TYHWRAS |
Miscellaneous databases
PROi | PR:P40046 |
RNActi | P40046, protein |
Family and domain databases
InterProi | View protein in InterPro IPR003807, DUF202 |
Pfami | View protein in Pfam PF02656, DUF202, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VTC1_YEAST | |
Accessioni | P40046Primary (citable) accession number: P40046 Secondary accession number(s): D3DLX7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - SIMILARITY comments
Index of protein domains and families