UniProtKB - P40016 (RPN3_YEAST)
Protein
26S proteasome regulatory subunit RPN3
Gene
RPN3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Miscellaneous
Present with 16700 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- enzyme regulator activity Source: InterPro
GO - Biological processi
- regulation of protein catabolic process Source: InterPro
- ubiquitin-dependent protein catabolic process Source: GO_Central
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit RPN3 |
Gene namesi | Name:RPN3 Synonyms:SUN2 Ordered Locus Names:YER021W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000823, RPN3 |
VEuPathDBi | FungiDB:YER021W |
Subcellular locationi
Other locations
- proteasome regulatory particle, lid subcomplex Source: SGD
Keywords - Cellular componenti
ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000173827 | 2 – 523 | 26S proteasome regulatory subunit RPN3Add BLAST | 522 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 454 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
N-acetylated by NAT1.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P40016 |
PaxDbi | P40016 |
PRIDEi | P40016 |
PTM databases
iPTMneti | P40016 |
Interactioni
Subunit structurei
The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity).
By similarityBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 36755, 133 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-1322N |
IntActi | P40016, 38 interactors |
MINTi | P40016 |
STRINGi | 4932.YER021W |
Miscellaneous databases
RNActi | P40016, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P40016 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 270 – 450 | PCIPROSITE-ProRule annotationAdd BLAST | 181 |
Sequence similaritiesi
Belongs to the proteasome subunit S3 family.Curated
Phylogenomic databases
eggNOGi | KOG2581, Eukaryota |
GeneTreei | ENSGT00940000153653 |
HOGENOMi | CLU_019858_1_2_1 |
InParanoidi | P40016 |
OMAi | HTFTLII |
Family and domain databases
InterProi | View protein in InterPro IPR013586, 26S_Psome_reg_C IPR000717, PCI_dom IPR035267, PSMD3 IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR10758:SF2, PTHR10758:SF2, 1 hit |
Pfami | View protein in Pfam PF01399, PCI, 1 hit PF08375, Rpn3_C, 1 hit |
SMARTi | View protein in SMART SM00088, PINT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P40016-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASTAVMMDV DSSGVNDLHH SEKKYAEEDQ VQELLKVLNE ISKTTLTLDP
60 70 80 90 100
RYIWRSLKDL SSLRNQELLN AETLCFTVNV LYPDSSSFKK NLLKFITSNH
110 120 130 140 150
KSSVPGSAEL RNSYPASFYS VNTEKKTIEV TAEINCFMHL LVQLFLWDSK
160 170 180 190 200
ELEQLVEFNR KVVIPNLLCY YNLRSLNLIN AKLWFYIYLS HETLARSSEE
210 220 230 240 250
INSDNQNIIL RSTMMKFLKI ASLKHDNETK AMLINLILRD FLNNGEVDSA
260 270 280 290 300
SDFISKLEYP HTDVSSSLEA RYFFYLSKIN AIQLDYSTAN EYIIAAIRKA
310 320 330 340 350
PHNSKSLGFL QQSNKLHCCI QLLMGDIPEL SFFHQSNMQK SLLPYYHLTK
360 370 380 390 400
AVKLGDLKKF TSTITKYKQL LLKDDTYQLC VRLRSNVIKT GIRIISLTYK
410 420 430 440 450
KISLRDICLK LNLDSEQTVE YMVSRAIRDG VIEAKINHED GFIETTELLN
460 470 480 490 500
IYDSEDPQQV FDERIKFANQ LHDEYLVSMR YPEDKKTQQN EKSENGENDD
510 520
DTLDGDLMDD MSDISDLDDL GFL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 355 | G → S no nucleotide entry (PubMed:8668124).Curated | 1 | |
Sequence conflicti | 355 | G → S in BAA11208 (PubMed:9017604).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D78023 Genomic DNA Translation: BAA11208.1 U18778 Genomic DNA Translation: AAB64554.1 BK006939 Genomic DNA Translation: DAA07674.1 |
PIRi | S50479 |
RefSeqi | NP_010938.1, NM_001178912.1 |
Genome annotation databases
EnsemblFungii | YER021W_mRNA; YER021W; YER021W |
GeneIDi | 856742 |
KEGGi | sce:YER021W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D78023 Genomic DNA Translation: BAA11208.1 U18778 Genomic DNA Translation: AAB64554.1 BK006939 Genomic DNA Translation: DAA07674.1 |
PIRi | S50479 |
RefSeqi | NP_010938.1, NM_001178912.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J47 | electron microscopy | - | S | 455-478 | [»] | |
3JCK | electron microscopy | 3.50 | A | 131-523 | [»] | |
3JCO | electron microscopy | 4.80 | S | 1-523 | [»] | |
3JCP | electron microscopy | 4.60 | S | 1-523 | [»] | |
4CR2 | electron microscopy | 7.70 | S | 1-523 | [»] | |
4CR3 | electron microscopy | 9.30 | S | 1-523 | [»] | |
4CR4 | electron microscopy | 8.80 | S | 1-523 | [»] | |
5A5B | electron microscopy | 9.50 | S | 1-523 | [»] | |
5MPB | electron microscopy | 7.80 | S | 1-523 | [»] | |
5MPC | electron microscopy | 7.70 | S | 1-523 | [»] | |
5MPD | electron microscopy | 4.10 | S | 1-523 | [»] | |
5MPE | electron microscopy | 4.50 | S | 1-523 | [»] | |
5WVI | electron microscopy | 6.30 | S | 1-523 | [»] | |
5WVK | electron microscopy | 4.20 | S | 1-523 | [»] | |
6FVT | electron microscopy | 4.10 | S | 18-492 | [»] | |
6FVU | electron microscopy | 4.50 | S | 18-492 | [»] | |
6FVV | electron microscopy | 5.40 | S | 18-492 | [»] | |
6FVW | electron microscopy | 4.50 | S | 18-492 | [»] | |
6FVX | electron microscopy | 4.90 | S | 18-492 | [»] | |
6FVY | electron microscopy | 6.10 | S | 18-492 | [»] | |
6J2C | electron microscopy | 7.00 | S | 1-523 | [»] | |
6J2N | electron microscopy | 7.50 | S | 1-523 | [»] | |
6J2Q | electron microscopy | 3.80 | S | 1-523 | [»] | |
6J2X | electron microscopy | 3.80 | S | 1-523 | [»] | |
6J30 | electron microscopy | 4.50 | S | 1-523 | [»] | |
SMRi | P40016 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36755, 133 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-1322N |
IntActi | P40016, 38 interactors |
MINTi | P40016 |
STRINGi | 4932.YER021W |
PTM databases
iPTMneti | P40016 |
Proteomic databases
MaxQBi | P40016 |
PaxDbi | P40016 |
PRIDEi | P40016 |
Genome annotation databases
EnsemblFungii | YER021W_mRNA; YER021W; YER021W |
GeneIDi | 856742 |
KEGGi | sce:YER021W |
Organism-specific databases
SGDi | S000000823, RPN3 |
VEuPathDBi | FungiDB:YER021W |
Phylogenomic databases
eggNOGi | KOG2581, Eukaryota |
GeneTreei | ENSGT00940000153653 |
HOGENOMi | CLU_019858_1_2_1 |
InParanoidi | P40016 |
OMAi | HTFTLII |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P40016 |
RNActi | P40016, protein |
Family and domain databases
InterProi | View protein in InterPro IPR013586, 26S_Psome_reg_C IPR000717, PCI_dom IPR035267, PSMD3 IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR10758:SF2, PTHR10758:SF2, 1 hit |
Pfami | View protein in Pfam PF01399, PCI, 1 hit PF08375, Rpn3_C, 1 hit |
SMARTi | View protein in SMART SM00088, PINT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS50250, PCI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPN3_YEAST | |
Accessioni | P40016Primary (citable) accession number: P40016 Secondary accession number(s): D3DLS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 5, 2010 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families