UniProtKB - P40009 (YND1_YEAST)
Protein
Golgi apyrase
Gene
YND1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Has equal high activity toward ADP/ATP, GDP/GTP, and UDP/UTP and approximately 50% less toward CDP/CTP and thiamine pyrophosphate. Has no activity toward GMP. Required for Golgi glycosylation and cell wall integrity. Together with CDC55, required for adenovirus E4orf4 (early region 4 open reading frame 4) induced toxicity, the apyrase activity is not required for this function. Plays a role in sphingolipid synthesis.3 Publications
Miscellaneous
Present with 450 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- a ribonucleoside 5'-triphosphate + 2 H2O = a ribonucleoside 5'-phosphate + 2 H+ + 2 phosphate2 PublicationsEC:3.6.1.52 Publications
Cofactori
Ca2+By similarity, Mg2+By similarity, Mn2+By similarityNote: A divalent cation Ca2+, Mg2+ or Mn2+.By similarity
Activity regulationi
Activity is inhibited both by interaction with VMA13 and by V-ATPase acidification of the lumen. The activity of VMA13 is not required for YND1 inhibition.1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 152 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- 8-oxo-dGTP phosphohydrolase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- dATP phosphohydrolase activity Source: UniProtKB-EC
- dCTP phosphohydrolase activity Source: UniProtKB-EC
- dGTP phosphohydrolase activity Source: UniProtKB-EC
- dTTP phosphohydrolase activity Source: UniProtKB-EC
- dUTP phosphohydrolase activity Source: UniProtKB-EC
- GTP phosphohydrolase activity Source: UniProtKB-EC
- nucleoside-diphosphatase activity Source: SGD
- nucleoside-triphosphatase activity Source: SGD
GO - Biological processi
- protein glycosylation Source: SGD
- sphingolipid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism, Sphingolipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-30192-MONOMER |
Reactomei | R-SCE-8850843, Phosphate bond hydrolysis by NTPDase proteins |
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Golgi apyrase (EC:3.6.1.5)Alternative name(s): ATP-diphosphatase ATP-diphosphohydrolase Adenosine diphosphatase Short name: ADPase Golgi nucleoside diphosphatase Yeast nucleoside diphosphatase 1 |
Gene namesi | Name:YND1 Ordered Locus Names:YER005W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000807, YND1 |
VEuPathDBi | FungiDB:YER005W |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 2 Publications
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Golgi apparatus
- Golgi apparatus Source: SGD
- Golgi membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 500 | LumenalSequence analysisAdd BLAST | 500 | |
Transmembranei | 501 – 517 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 518 – 630 | CytoplasmicSequence analysisAdd BLAST | 113 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Cells are partially resistant to E4orf4 and can partially suppress the spindle checkpoint defect in CDC55 deletion mutant. Does not suppress the rapamycin-resistance of CDC55 deletion mutant. YND1 and CDC55 double mutant is fully resistant to E4orf4. Deletion mutant suppresses the synthetic lethality of triple mutants, where UPC2 and ECM22 are knocked out along with either HAP1, ERG6 or ERG28.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 152 | E → Q: Expressed at lower levels. GTPase activity reduced. 1 Publication | 1 | |
Mutagenesisi | 189 | S → A: Expressed at the same level as wild-type. GTPase and GDPase activities decreased more than 20-fold toward GTP and 3-fold for GDP. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209920 | 1 – 630 | Golgi apyraseAdd BLAST | 630 |
Proteomic databases
MaxQBi | P40009 |
PaxDbi | P40009 |
PRIDEi | P40009 |
PTM databases
iPTMneti | P40009 |
Interactioni
Subunit structurei
Interacts with activator subunit VMA13 of vacuolar H+-ATPase.
Interacts with CDC55; this interaction is disrupted by adenovirus E4orf4, which remains associated with both YND1 and CDC55.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 36735, 98 interactors |
DIPi | DIP-7607N |
IntActi | P40009, 8 interactors |
MINTi | P40009 |
STRINGi | 4932.YER005W |
Miscellaneous databases
RNActi | P40009, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the GDA1/CD39 NTPase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1386, Eukaryota |
GeneTreei | ENSGT01010000222402 |
HOGENOMi | CLU_010246_3_3_1 |
InParanoidi | P40009 |
OMAi | CDVEHTQ |
Family and domain databases
InterProi | View protein in InterPro IPR000407, GDA1_CD39_NTPase |
PANTHERi | PTHR11782, PTHR11782, 1 hit |
Pfami | View protein in Pfam PF01150, GDA1_CD39, 1 hit |
PROSITEi | View protein in PROSITE PS01238, GDA1_CD39_NTPASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P40009-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLIENTNDRF GIVIDAGSSG SRIHVFKWQD TESLLHATNQ DSQSILQSVP
60 70 80 90 100
HIHQEKDWTF KLNPGLSSFE KKPQDAYKSH IKPLLDFAKN IIPESHWSSC
110 120 130 140 150
PVFIQATAGM RLLPQDIQSS ILDGLCQGLK HPAEFLVEDC SAQIQVIDGE
160 170 180 190 200
TEGLYGWLGL NYLYGHFNDY NPEVSDHFTF GFMDMGGAST QIAFAPHDSG
210 220 230 240 250
EIARHRDDIA TIFLRSVNGD LQKWDVFVST WLGFGANQAR RRYLAQLINT
260 270 280 290 300
LPENTNDYEN DDFSTRNLND PCMPRGSSTD FEFKDTIFHI AGSGNYEQCT
310 320 330 340 350
KSIYPLLLKN MPCDDEPCLF NGVHAPRIDF ANDKFIGTSE YWYTANDVFK
360 370 380 390 400
LGGEYNFDKF SKSLREFCNS NWTQILANSD KGVYNSIPEN FLKDACFKGN
410 420 430 440 450
WVLNILHEGF DMPRIDVDAE NVNDRPLFQS VEKVEERELS WTLGRILLYA
460 470 480 490 500
SGSILAGNDD FMVGIAPSER RTKLTGKKFI PGKLLESDQL RKQSSSLSNK
510 520 530 540 550
GFLMWFAIIC CIFYLIFHRS HIIRRRFSGL YNITKDFKTG IRRRLKFLRR
560 570 580 590 600
SDPFSRLEEG ELGTDVDGFK DVYRMKSSSM FDLGKSSATM QREHEPQRTA
610 620 630
SQSANLAPSN LRPAFSMADF SKFKDSRLYD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 498 | S → P in AAT92897 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF203695 Genomic DNA Translation: AAF17573.1 U18778 Genomic DNA Translation: AAB64538.1 AY692878 Genomic DNA Translation: AAT92897.1 BK006939 Genomic DNA Translation: DAA07655.1 |
PIRi | S50463 |
RefSeqi | NP_010920.3, NM_001178896.3 |
Genome annotation databases
EnsemblFungii | YER005W_mRNA; YER005W; YER005W |
GeneIDi | 856722 |
KEGGi | sce:YER005W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF203695 Genomic DNA Translation: AAF17573.1 U18778 Genomic DNA Translation: AAB64538.1 AY692878 Genomic DNA Translation: AAT92897.1 BK006939 Genomic DNA Translation: DAA07655.1 |
PIRi | S50463 |
RefSeqi | NP_010920.3, NM_001178896.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36735, 98 interactors |
DIPi | DIP-7607N |
IntActi | P40009, 8 interactors |
MINTi | P40009 |
STRINGi | 4932.YER005W |
PTM databases
iPTMneti | P40009 |
Proteomic databases
MaxQBi | P40009 |
PaxDbi | P40009 |
PRIDEi | P40009 |
Genome annotation databases
EnsemblFungii | YER005W_mRNA; YER005W; YER005W |
GeneIDi | 856722 |
KEGGi | sce:YER005W |
Organism-specific databases
SGDi | S000000807, YND1 |
VEuPathDBi | FungiDB:YER005W |
Phylogenomic databases
eggNOGi | KOG1386, Eukaryota |
GeneTreei | ENSGT01010000222402 |
HOGENOMi | CLU_010246_3_3_1 |
InParanoidi | P40009 |
OMAi | CDVEHTQ |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | MetaCyc:G3O-30192-MONOMER |
Reactomei | R-SCE-8850843, Phosphate bond hydrolysis by NTPDase proteins |
Miscellaneous databases
PROi | PR:P40009 |
RNActi | P40009, protein |
Family and domain databases
InterProi | View protein in InterPro IPR000407, GDA1_CD39_NTPase |
PANTHERi | PTHR11782, PTHR11782, 1 hit |
Pfami | View protein in Pfam PF01150, GDA1_CD39, 1 hit |
PROSITEi | View protein in PROSITE PS01238, GDA1_CD39_NTPASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YND1_YEAST | |
Accessioni | P40009Primary (citable) accession number: P40009 Secondary accession number(s): D3DLQ1, Q6B252 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families