UniProtKB - P39998 (EDC3_YEAST)
Protein
Enhancer of mRNA-decapping protein 3
Gene
EDC3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Stimulates decapping of both stable and unstable mRNA during mRNA decay. Does not affect nonsense-mediated mRNA decay. Required for normal P-body assembly.2 Publications
Miscellaneous
Present with 2340 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- mRNA binding Source: SGD
GO - Biological processi
- cellular response to osmotic stress Source: SGD
- deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
- P-body assembly Source: SGD
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD
Enzyme and pathway databases
Reactomei | R-SCE-430039, mRNA decay by 5' to 3' exoribonuclease |
Names & Taxonomyi
Protein namesi | Recommended name: Enhancer of mRNA-decapping protein 3 |
Gene namesi | Name:EDC3 Synonyms:LSM16 Ordered Locus Names:YEL015W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YEL015W |
SGDi | S000000741, EDC3 |
Subcellular locationi
Other locations
- P-body 1 Publication
Note: Is concentrated in several cytoplasmic foci called P bodies (or cytoplasmic processing bodies) which represent sites of mRNA decapping and 5' to 3' exonucleotidic decay.
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 360 – 361 | RH → AA: Abolishes homodimerization. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202613 | 1 – 551 | Enhancer of mRNA-decapping protein 3Add BLAST | 551 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 257 | PhosphoserineCombined sources | 1 | |
Modified residuei | 261 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P39998 |
PaxDbi | P39998 |
PRIDEi | P39998 |
PTM databases
iPTMneti | P39998 |
Interactioni
Subunit structurei
Homodimer.
Interacts with DCP2.
1 PublicationBinary interactionsi
Hide detailsP39998
With | #Exp. | IntAct |
---|---|---|
DCP1 [Q12517] | 4 | EBI-22300,EBI-38519 |
DCP2 [P53550] | 6 | EBI-22300,EBI-270 |
DHH1 [P39517] | 3 | EBI-22300,EBI-158 |
Protein-protein interaction databases
BioGRIDi | 36716, 90 interactors |
DIPi | DIP-842N |
IntActi | P39998, 24 interactors |
MINTi | P39998 |
STRINGi | 4932.YEL015W |
Miscellaneous databases
RNActi | P39998, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P39998 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 93 – 129 | DFDFPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 288 – 527 | YjeF N-terminalPROSITE-ProRule annotationAdd BLAST | 240 |
Sequence similaritiesi
Belongs to the EDC3 family.Curated
Phylogenomic databases
eggNOGi | KOG2585, Eukaryota |
GeneTreei | ENSGT00390000016435 |
HOGENOMi | CLU_037134_0_0_1 |
InParanoidi | P39998 |
OMAi | NLAMFDK |
Family and domain databases
Gene3Di | 3.40.50.10260, 1 hit |
InterProi | View protein in InterPro IPR025762, DFDF IPR019050, FDF_dom IPR004443, YjeF_N_dom IPR036652, YjeF_N_dom_sf |
Pfami | View protein in Pfam PF09532, FDF, 1 hit PF03853, YjeF_N, 1 hit |
SMARTi | View protein in SMART SM01199, FDF, 1 hit |
SUPFAMi | SSF64153, SSF64153, 1 hit |
PROSITEi | View protein in PROSITE PS51512, DFDF, 1 hit PS51385, YJEF_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P39998-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSQFVGFGVQ VELKDGKLIQ GKIAKATSKG LTLNDVQFGD GGKSQAFKVR
60 70 80 90 100
ASRLKDLKVL TVASQSGKRK QQRQQQQQND YNQNRGEHID WQDDDVSKIK
110 120 130 140 150
QQEDFDFQRN LGMFNKKDVF AQLKQNDDIL PENRLQGHNR KQTQLQQNNY
160 170 180 190 200
QNDELVIPDA KKDSWNKISS RNEQSTHQSQ PQQDAQDDLV LEDDEHEYDV
210 220 230 240 250
DDIDDPKYLP ITQSLNITHL IHSATNSPSI NDKTKGTVIN DKDQVLAKLG
260 270 280 290 300
QMIISQSRSN STSLPAANKQ TTIRSKNTKQ NIPMATPVQL LEMESITSEF
310 320 330 340 350
FSINSAGLLE NFAVNASFFL KQKLGGRARL RLQNSNPEPL VVILASDSNR
360 370 380 390 400
SGAKALALGR HLCQTGHIRV ITLFTCSQNE LQDSMVKKQT DIYKKCGGKI
410 420 430 440 450
VNSVSSLESA METLNSPVEI VIDAMQGYDC TLSDLAGTSE VIESRIKSMI
460 470 480 490 500
SWCNKQRGST KVWSLDIPNG FDAGSGMPDI FFSDRIEATG IICSGWPLIA
510 520 530 540 550
INNLIANLPS LEDAVLIDIG IPQGAYSQRT SLRKFQNCDL FVTDGSLLLD
L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18530 Genomic DNA Translation: AAB64492.1 BK006939 Genomic DNA Translation: DAA07639.1 |
PIRi | S50444 |
RefSeqi | NP_010901.1, NM_001178830.1 |
Genome annotation databases
EnsemblFungii | YEL015W_mRNA; YEL015W; YEL015W |
GeneIDi | 856700 |
KEGGi | sce:YEL015W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18530 Genomic DNA Translation: AAB64492.1 BK006939 Genomic DNA Translation: DAA07639.1 |
PIRi | S50444 |
RefSeqi | NP_010901.1, NM_001178830.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4BRU | X-ray | 3.24 | B | 77-116 | [»] | |
6Y3Z | X-ray | 3.49 | C | 1-66 | [»] | |
SMRi | P39998 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36716, 90 interactors |
DIPi | DIP-842N |
IntActi | P39998, 24 interactors |
MINTi | P39998 |
STRINGi | 4932.YEL015W |
PTM databases
iPTMneti | P39998 |
Proteomic databases
MaxQBi | P39998 |
PaxDbi | P39998 |
PRIDEi | P39998 |
Genome annotation databases
EnsemblFungii | YEL015W_mRNA; YEL015W; YEL015W |
GeneIDi | 856700 |
KEGGi | sce:YEL015W |
Organism-specific databases
EuPathDBi | FungiDB:YEL015W |
SGDi | S000000741, EDC3 |
Phylogenomic databases
eggNOGi | KOG2585, Eukaryota |
GeneTreei | ENSGT00390000016435 |
HOGENOMi | CLU_037134_0_0_1 |
InParanoidi | P39998 |
OMAi | NLAMFDK |
Enzyme and pathway databases
Reactomei | R-SCE-430039, mRNA decay by 5' to 3' exoribonuclease |
Miscellaneous databases
PROi | PR:P39998 |
RNActi | P39998, protein |
Family and domain databases
Gene3Di | 3.40.50.10260, 1 hit |
InterProi | View protein in InterPro IPR025762, DFDF IPR019050, FDF_dom IPR004443, YjeF_N_dom IPR036652, YjeF_N_dom_sf |
Pfami | View protein in Pfam PF09532, FDF, 1 hit PF03853, YjeF_N, 1 hit |
SMARTi | View protein in SMART SM01199, FDF, 1 hit |
SUPFAMi | SSF64153, SSF64153, 1 hit |
PROSITEi | View protein in PROSITE PS51512, DFDF, 1 hit PS51385, YJEF_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EDC3_YEAST | |
Accessioni | P39998Primary (citable) accession number: P39998 Secondary accession number(s): D3DLN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD