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Protein

Manganese-transporting ATPase 1

Gene

SPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.1 Publication

Miscellaneous

Present with 1870 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4874-aspartylphosphate intermediateBy similarity1
Metal bindingi816MagnesiumBy similarity1
Metal bindingi820MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular calcium ion homeostasis Source: SGD
  • cellular manganese ion homeostasis Source: SGD
  • sterol homeostasis Source: SGD
  • transmembrane transport Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30153-MONOMER
ReactomeiR-SCE-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.10.3 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese-transporting ATPase 1 (EC:3.6.3.-)
Gene namesi
Name:SPF1
Ordered Locus Names:YEL031W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL031W
SGDiS000000757 SPF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 43HelicalSequence analysisAdd BLAST22
Topological domaini44 – 49ExtracellularSequence analysis6
Transmembranei50 – 72HelicalSequence analysisAdd BLAST23
Topological domaini73 – 191CytoplasmicSequence analysisAdd BLAST119
Transmembranei192 – 214HelicalSequence analysisAdd BLAST23
Topological domaini215 – 217ExtracellularSequence analysis3
Transmembranei218 – 236HelicalSequence analysisAdd BLAST19
Topological domaini237 – 399CytoplasmicSequence analysisAdd BLAST163
Transmembranei400 – 419HelicalSequence analysisAdd BLAST20
Topological domaini420 – 432ExtracellularSequence analysisAdd BLAST13
Transmembranei433 – 454HelicalSequence analysisAdd BLAST22
Topological domaini455 – 993CytoplasmicSequence analysisAdd BLAST539
Transmembranei994 – 1013HelicalSequence analysisAdd BLAST20
Topological domaini1014 – 1020ExtracellularSequence analysis7
Transmembranei1021 – 1037HelicalSequence analysisAdd BLAST17
Topological domaini1038 – 1055CytoplasmicSequence analysisAdd BLAST18
Transmembranei1056 – 1079HelicalSequence analysisAdd BLAST24
Topological domaini1080 – 1099ExtracellularSequence analysisAdd BLAST20
Transmembranei1100 – 1122HelicalSequence analysisAdd BLAST23
Topological domaini1123 – 1133CytoplasmicSequence analysisAdd BLAST11
Transmembranei1134 – 1153HelicalSequence analysisAdd BLAST20
Topological domaini1154 – 1170ExtracellularSequence analysisAdd BLAST17
Transmembranei1171 – 1193HelicalSequence analysisAdd BLAST23
Topological domaini1194 – 1215CytoplasmicSequence analysisAdd BLAST22

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Specific reduction in the luminal concentration of manganese ions, and increase of cytosolic Mn2+-dependent processes. Interruption of sphingolipids biosynthesis, reduced exit of GPI-anchored proteins from the ER, reduced protein mannosylation. Overexpression leads to an increase of luminal manganese.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi487D → N: Loss of ATPase activity and transport function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463491 – 1215Manganese-transporting ATPase 1Add BLAST1215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei324PhosphoserineCombined sources1
Modified residuei936PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39986
PaxDbiP39986
PRIDEiP39986

PTM databases

CarbonylDBiP39986
iPTMnetiP39986

Interactioni

Protein-protein interaction databases

BioGridi36698, 457 interactors
DIPiDIP-6637N
IntActiP39986, 10 interactors
STRINGi4932.YEL031W

Structurei

3D structure databases

ProteinModelPortaliP39986
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000075064
HOGENOMiHOG000199432
InParanoidiP39986
KOiK14950
OMAiQVSTFAI
OrthoDBiEOG092C0IZK

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006544 P-type_TPase_V
IPR001757 P_typ_ATPase
SUPFAMiSSF56784 SSF56784, 3 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01494 ATPase_P-type, 2 hits
TIGR01657 P-ATPase-V, 1 hit
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

P39986-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKSFVSSP IVRDSTLLVP KSLIAKPYVL PFFPLYATFA QLYFQQYDRY
60 70 80 90 100
IKGPEWTFVY LGTLVSLNIL VMLMPAWNVK IKAKFNYSTT KNVNEATHIL
110 120 130 140 150
IYTTPNNGSD GIVEIQRVTE AGSLQTFFQF QKKRFLWHEN EQVFSSPKFL
160 170 180 190 200
VDESPKIGDF QKCKGHSGDL THLKRLYGEN SFDIPIPTFM ELFKEHAVAP
210 220 230 240 250
LFVFQVFCVA LWLLDEFWYY SLFNLFMIIS MEAAAVFQRL TALKEFRTMG
260 270 280 290 300
IKPYTINVFR NKKWVALQTN ELLPMDLVSI TRTAEESAIP CDLILLDGSA
310 320 330 340 350
IVNEAMLSGE STPLLKESIK LRPSEDNLQL DGVDKIAVLH GGTKALQVTP
360 370 380 390 400
PEHKSDIPPP PDGGALAIVT KTGFETSQGS LVRVMIYSAE RVSVDNKEAL
410 420 430 440 450
MFILFLLIFA VIASWYVWVE GTKMGRIQSK LILDCILIIT SVVPPELPME
460 470 480 490 500
LTMAVNSSLA ALAKFYVYCT EPFRIPFAGR IDVCCFDKTG TLTGEDLVFE
510 520 530 540 550
GLAGISADSE NIRHLYSAAE APESTILVIG AAHALVKLED GDIVGDPMEK
560 570 580 590 600
ATLKAVGWAV ERKNSNYREG TGKLDIIRRF QFSSALKRSA SIASHNDALF
610 620 630 640 650
AAVKGAPETI RERLSDIPKN YDEIYKSFTR SGSRVLALAS KSLPKMSQSK
660 670 680 690 700
IDDLNRDDVE SELTFNGFLI FHCPLKDDAI ETIKMLNESS HRSIMITGDN
710 720 730 740 750
PLTAVHVAKE VGIVFGETLI LDRAGKSDDN QLLFRDVEET VSIPFDPSKD
760 770 780 790 800
TFDHSKLFDR YDIAVTGYAL NALEGHSQLR DLLRHTWVYA RVSPSQKEFL
810 820 830 840 850
LNTLKDMGYQ TLMCGDGTND VGALKQAHVG IALLNGTEEG LKKLGEQRRL
860 870 880 890 900
EGMKMMYIKQ TEFMARWNQP QPPVPEPIAH LFPPGPKNPH YLKALESKGT
910 920 930 940 950
VITPEIRKAV EEANSKPVEV IKPNGLSEKK PADLASLLLN SAGDAQGDEA
960 970 980 990 1000
PALKLGDASC AAPFTSKLAN VSAVTNIIRQ GRCALVNTIQ MYKILALNCL
1010 1020 1030 1040 1050
ISAYSLSIIY MAGVKFGDGQ ATVSGLLLSV CFLSISRGKP LEKLSKQRPQ
1060 1070 1080 1090 1100
SGIFNVYIMG SILSQFAVHI ATLVYITTEI YKLEPREPQV DLEKEFAPSL
1110 1120 1130 1140 1150
LNTGIFIIQL VQQVSTFAVN YQGEPFRENI RSNKGMYYGL LGVTGLALAS
1160 1170 1180 1190 1200
ATEFLPELNE AMKFVPMTDD FKIKLTLTLL LDFFGSWGVE HFFKFFFMDD
1210
KPSDISVQQV KIASK
Length:1,215
Mass (Da):135,269
Last modified:February 1, 1995 - v1
Checksum:i7A9960D34B91B5AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18530 Genomic DNA Translation: AAB64508.1
BK006939 Genomic DNA Translation: DAA07622.1
PIRiS50428
RefSeqiNP_010883.3, NM_001178846.3

Genome annotation databases

EnsemblFungiiYEL031W; YEL031W; YEL031W
GeneIDi856681
KEGGisce:YEL031W

Similar proteinsi

Entry informationi

Entry nameiATC6_YEAST
AccessioniPrimary (citable) accession number: P39986
Secondary accession number(s): D3DLL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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