Vacuolar protein 8

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• identical protein binding Source: IntAct <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Mechanistic insight into the nucleus-vacuole junction based on the Vac8p-Nvj1p crystal structure."
    Jeong H., Park J., Kim H.I., Lee M., Ko Y.J., Lee S., Jun Y., Lee C.
    Proc Natl Acad Sci U S A 114:E4539-E4548(2017) [PubMed] [Europe PMC] [Abstract]
• protein-membrane adaptor activity Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole."
    Tang F., Kauffman E.J., Novak J.L., Nau J.J., Catlett N.L., Weisman L.S.
    Nature 422:87-92(2003) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• autophagosome assembly Source: SGDInferred from mutant phenotypeⁱ
  • "Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae."
    Hollenstein D.M., Gomez-Sanchez R., Ciftci A., Kriegenburg F., Mari M., Torggler R., Licheva M., Reggiori F., Kraft C.
    J Cell Sci 132:0-0(2019) [PubMed] [Europe PMC] [Abstract]
• autophagy Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Vac8 Controls Vacuolar Membrane Dynamics during Different Autophagy Pathways in Saccharomyces cerevisiae."
    Boutouja F., Stiehm C.M., Reidick C., Mastalski T., Brinkmeier R., Magraoui F.E., Platta H.W.
    Cells 8:0-0(2019) [PubMed] [Europe PMC] [Abstract]
• Cvt vesicle assembly Source: SGDInferred from mutant phenotypeⁱ
  • Ref.5

    "Apg13p and Vac8p are part of a complex of phosphoproteins that are required for cytoplasm to vacuole targeting."
    Scott S.V., Nice D.C. III, Nau J.J., Weisman L.S., Kamada Y., Keizer-Gunnink I., Funakoshi T., Veenhuis M., Ohsumi Y., Klionsky D.J.
    J. Biol. Chem. 275:25840-25849(2000) [PubMed] [Europe PMC] [Abstract]

    Cited for: PHOSPHORYLATION, INTERACTION WITH ATG13.
• macroautophagy Source: SGDInferred from mutant phenotypeⁱ
  • "Atg17 regulates the magnitude of the autophagic response."
    Cheong H., Yorimitsu T., Reggiori F., Legakis J.E., Wang C.W., Klionsky D.J.
    Mol. Biol. Cell 16:3438-3453(2005) [PubMed] [Europe PMC] [Abstract]
• nucleus-vacuole junction assembly Source: SGDInferred from mutant phenotypeⁱ
  • "Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p."
    Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S., Goldfarb D.S.
    Mol. Biol. Cell 11:2445-2457(2000) [PubMed] [Europe PMC] [Abstract]
• pexophagy Source: SGDInferred from direct assayⁱ
  • "Vac8 Controls Vacuolar Membrane Dynamics during Different Autophagy Pathways in Saccharomyces cerevisiae."
    Boutouja F., Stiehm C.M., Reidick C., Mastalski T., Brinkmeier R., Magraoui F.E., Platta H.W.
    Cells 8:0-0(2019) [PubMed] [Europe PMC] [Abstract]
• piecemeal microautophagy of the nucleus Source: SGDInferred from mutant phenotypeⁱ
  • "Piecemeal microautophagy of nucleus in Saccharomyces cerevisiae."
    Roberts P., Moshitch-Moshkovitz S., Kvam E., O'Toole E., Winey M., Goldfarb D.S.
    Mol. Biol. Cell 14:129-141(2003) [PubMed] [Europe PMC] [Abstract]
  • "Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
    Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
    Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
• protein localization to membrane raft Source: SGDInferred from mutant phenotypeⁱ
  • "Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p."
    Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S., Goldfarb D.S.
    Mol. Biol. Cell 11:2445-2457(2000) [PubMed] [Europe PMC] [Abstract]
• ribophagy Source: SGDInferred from direct assayⁱ
  • "Vac8 Controls Vacuolar Membrane Dynamics during Different Autophagy Pathways in Saccharomyces cerevisiae."
    Boutouja F., Stiehm C.M., Reidick C., Mastalski T., Brinkmeier R., Magraoui F.E., Platta H.W.
    Cells 8:0-0(2019) [PubMed] [Europe PMC] [Abstract]
• vacuole fusion, non-autophagic Source: SGDInferred from mutant phenotypeⁱ
  • "Fusion of docked membranes requires the armadillo repeat protein Vac8p."
    Wang Y.X., Kauffman E.J., Duex J.E., Weisman L.S.
    J Biol Chem 276:35133-35140(2001) [PubMed] [Europe PMC] [Abstract]
  • "The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8."
    Hou H., Subramanian K., LaGrassa T.J., Markgraf D., Dietrich L.E., Urban J., Decker N., Ungermann C.
    Proc Natl Acad Sci U S A 102:17366-17371(2005) [PubMed] [Europe PMC] [Abstract]
• vacuole inheritance Source: SGDInferred from mutant phenotypeⁱ
  • "Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through the direct interaction of Vac8p with Nvj1p."
    Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S., Goldfarb D.S.
    Mol. Biol. Cell 11:2445-2457(2000) [PubMed] [Europe PMC] [Abstract]
  • "The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8."
    Hou H., Subramanian K., LaGrassa T.J., Markgraf D., Dietrich L.E., Urban J., Decker N., Ungermann C.
    Proc Natl Acad Sci U S A 102:17366-17371(2005) [PubMed] [Europe PMC] [Abstract]
  • "Multiple classes of yeast mutants are defective in vacuole partitioning yet target vacuole proteins correctly."
    Wang Y.X., Zhao H., Harding T.M., Gomes de Mesquita D.S., Woldringh C.L., Klionsky D.J., Munn A.L., Weisman L.S.
    Mol Biol Cell 7:1375-1389(1996) [PubMed] [Europe PMC] [Abstract]

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationⁱ

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• identical protein binding Source: IntActInferred from physical interactionⁱ
  • "Mechanistic insight into the nucleus-vacuole junction based on the Vac8p-Nvj1p crystal structure."
    Jeong H., Park J., Kim H.I., Lee M., Ko Y.J., Lee S., Jun Y., Lee C.
    Proc Natl Acad Sci U S A 114:E4539-E4548(2017) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK 
        60         70         80         90        100
ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ 
       110        120        130        140        150
IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI 
       160        170        180        190        200
TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN 
       210        220        230        240        250
RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP 
       260        270        280        290        300
RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL 
       310        320        330        340        350
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI 
       360        370        380        390        400
QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF 
       410        420        430        440        450
AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN 
       460        470        480        490        500
YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE 
       510        520        530        540        550
DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN 
       560        570 
DTGSEHQPVE DASLELYNIT QQILQFLH                         

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. Yeast chromosome V
   Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
4. SIMILARITY comments
   Index of protein domains and families