UniProtKB - P39968 (VAC8_YEAST)
Protein
Vacuolar protein 8
Gene
VAC8
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.1 Publication
Miscellaneous
Present with 5680 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein-membrane adaptor activity Source: SGD
GO - Biological processi
- autophagosome assembly Source: SGD
- autophagy Source: SGD
- Cvt vesicle assembly Source: SGD
- macroautophagy Source: SGD
- nucleus-vacuole junction assembly Source: SGD
- pexophagy Source: SGD
- piecemeal microautophagy of the nucleus Source: SGD
- protein localization to membrane raft Source: SGD
- ribophagy Source: SGD
- vacuole fusion, non-autophagic Source: SGD
- vacuole inheritance Source: SGD
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein 8 |
Gene namesi | Name:VAC8 Synonyms:YEB3 Ordered Locus Names:YEL013W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YEL013W |
SGDi | S000000739, VAC8 |
Subcellular locationi
Vacuole
- Vacuole membrane 1 Publication; Lipid-anchor 1 Publication
Cytosol
- cytosol Source: SGD
Vacuole
- fungal-type vacuole membrane Source: SGD
Other locations
- integral component of membrane Source: SGD
- membrane raft Source: SGD
- Myo2p-Vac17p-Vac8p transport complex Source: SGD
- nucleus-vacuole junction Source: SGD
- phagophore assembly site Source: SGD
Keywords - Cellular componenti
Membrane, VacuolePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 4 – 7 | CCSC → GTSS in VAC8-3; not palmitoylated. 1 Publication | 4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000064302 | 2 – 578 | Vacuolar protein 8Add BLAST | 577 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Lipidationi | 4 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 5 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 7 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 11 | PhosphoserineCombined sources | 1 | |
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Cross-linki | 77 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Cross-linki | 515 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Post-translational modificationi
Palmitoylated on one or more of its N-terminal cysteine residues by PFA3, which is required for vacuole fusion.3 Publications
Keywords - PTMi
Isopeptide bond, Lipoprotein, Myristate, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P39968 |
PaxDbi | P39968 |
PRIDEi | P39968 |
PTM databases
iPTMneti | P39968 |
SwissPalmi | P39968 |
Interactioni
Binary interactionsi
Hide detailsP39968
With | #Exp. | IntAct |
---|---|---|
ATG13 [Q06628] | 7 | EBI-20212,EBI-36188 |
NVJ1 [P38881] | 10 | EBI-20212,EBI-24885 |
VAB2 [P40003] | 3 | EBI-20212,EBI-22275 |
VAC17 [P25591] | 3 | EBI-20212,EBI-21800 |
itself | 2 | EBI-20212,EBI-20212 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 36717, 333 interactors |
ComplexPortali | CPX-1304, MYO2-VAC17-VAC8 transport complex CPX-1381, NVJ1-VAC8 nucleus-vacuole junction complex |
DIPi | DIP-5121N |
IntActi | P39968, 29 interactors |
MINTi | P39968 |
STRINGi | 4932.YEL013W |
Miscellaneous databases
RNActi | P39968, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P39968 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 37 – 75 | ARM 1Add BLAST | 39 | |
Repeati | 76 – 114 | ARM 2Add BLAST | 39 | |
Repeati | 116 – 155 | ARM 3Add BLAST | 40 | |
Repeati | 157 – 196 | ARM 4Add BLAST | 40 | |
Repeati | 198 – 237 | ARM 5Add BLAST | 40 | |
Repeati | 239 – 280 | ARM 6Add BLAST | 42 | |
Repeati | 282 – 321 | ARM 7Add BLAST | 40 | |
Repeati | 323 – 363 | ARM 8Add BLAST | 41 | |
Repeati | 407 – 446 | ARM 9Add BLAST | 40 |
Sequence similaritiesi
Belongs to the beta-catenin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4224, Eukaryota |
GeneTreei | ENSGT00940000167949 |
HOGENOMi | CLU_021483_0_0_1 |
InParanoidi | P39968 |
OMAi | ISIHPMN |
Family and domain databases
Gene3Di | 1.25.10.10, 3 hits |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo |
Pfami | View protein in Pfam PF00514, Arm, 8 hits |
SMARTi | View protein in SMART SM00185, ARM, 9 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 7 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P39968-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSCCSCLKD SSDEASVSPI ADNEREAVTL LLGYLEDKDQ LDFYSGGPLK
60 70 80 90 100
ALTTLVYSDN LNLQRSAALA FAEITEKYVR QVSREVLEPI LILLQSQDPQ
110 120 130 140 150
IQVAACAALG NLAVNNENKL LIVEMGGLEP LINQMMGDNV EVQCNAVGCI
160 170 180 190 200
TNLATRDDNK HKIATSGALI PLTKLAKSKH IRVQRNATGA LLNMTHSEEN
210 220 230 240 250
RKELVNAGAV PVLVSLLSST DPDVQYYCTT ALSNIAVDEA NRKKLAQTEP
260 270 280 290 300
RLVSKLVSLM DSPSSRVKCQ ATLALRNLAS DTSYQLEIVR AGGLPHLVKL
310 320 330 340 350
IQSDSIPLVL ASVACIRNIS IHPLNEGLIV DAGFLKPLVR LLDYKDSEEI
360 370 380 390 400
QCHAVSTLRN LAASSEKNRK EFFESGAVEK CKELALDSPV SVQSEISACF
410 420 430 440 450
AILALADVSK LDLLEANILD ALIPMTFSQN QEVSGNAAAA LANLCSRVNN
460 470 480 490 500
YTKIIEAWDR PNEGIRGFLI RFLKSDYATF EHIALWTILQ LLESHNDKVE
510 520 530 540 550
DLVKNDDDII NGVRKMADVT FERLQRSGID VKNPGSNNNP SSNDNNSNNN
560 570
DTGSEHQPVE DASLELYNIT QQILQFLH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF005267 mRNA Translation: AAQ13402.1 U18530 Genomic DNA Translation: AAB64490.1 BK006939 Genomic DNA Translation: DAA07640.1 |
PIRi | S50446 |
RefSeqi | NP_010903.3, NM_001178828.3 |
Genome annotation databases
EnsemblFungii | YEL013W_mRNA; YEL013W; YEL013W |
GeneIDi | 856702 |
KEGGi | sce:YEL013W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF005267 mRNA Translation: AAQ13402.1 U18530 Genomic DNA Translation: AAB64490.1 BK006939 Genomic DNA Translation: DAA07640.1 |
PIRi | S50446 |
RefSeqi | NP_010903.3, NM_001178828.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5XJG | X-ray | 2.40 | A/C | 10-515 | [»] | |
6KBM | X-ray | 2.90 | A | 10-515 | [»] | |
6KBN | X-ray | 3.20 | A/C | 1-578 | [»] | |
SMRi | P39968 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36717, 333 interactors |
ComplexPortali | CPX-1304, MYO2-VAC17-VAC8 transport complex CPX-1381, NVJ1-VAC8 nucleus-vacuole junction complex |
DIPi | DIP-5121N |
IntActi | P39968, 29 interactors |
MINTi | P39968 |
STRINGi | 4932.YEL013W |
PTM databases
iPTMneti | P39968 |
SwissPalmi | P39968 |
Proteomic databases
MaxQBi | P39968 |
PaxDbi | P39968 |
PRIDEi | P39968 |
Genome annotation databases
EnsemblFungii | YEL013W_mRNA; YEL013W; YEL013W |
GeneIDi | 856702 |
KEGGi | sce:YEL013W |
Organism-specific databases
EuPathDBi | FungiDB:YEL013W |
SGDi | S000000739, VAC8 |
Phylogenomic databases
eggNOGi | KOG4224, Eukaryota |
GeneTreei | ENSGT00940000167949 |
HOGENOMi | CLU_021483_0_0_1 |
InParanoidi | P39968 |
OMAi | ISIHPMN |
Miscellaneous databases
PROi | PR:P39968 |
RNActi | P39968, protein |
Family and domain databases
Gene3Di | 1.25.10.10, 3 hits |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000225, Armadillo |
Pfami | View protein in Pfam PF00514, Arm, 8 hits |
SMARTi | View protein in SMART SM00185, ARM, 9 hits |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50176, ARM_REPEAT, 7 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VAC8_YEAST | |
Accessioni | P39968Primary (citable) accession number: P39968 Secondary accession number(s): D3DLN6, Q549T0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD