UniProtKB - P39951 (CDK1_RAT)
Cyclin-dependent kinase 1
Cdk1
Functioni
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 128 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: RGD
- cyclin binding Source: RGD
- cyclin-dependent protein kinase activity Source: RGD
- cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- histone kinase activity Source: RGD
- Hsp70 protein binding Source: RGD
- kinase activity Source: RGD
- protein kinase activity Source: RGD
- protein serine/threonine kinase activity Source: ParkinsonsUK-UCL
- RNA polymerase II CTD heptapeptide repeat kinase activity Source: RGD
GO - Biological processi
- animal organ regeneration Source: RGD
- apoptotic process Source: UniProtKB-KW
- cell aging Source: RGD
- cell division Source: UniProtKB-KW
- cell population proliferation Source: RGD
- cellular response to hydrogen peroxide Source: RGD
- chromosome condensation Source: RGD
- epithelial cell differentiation Source: RGD
- G2/M transition of mitotic cell cycle Source: GO_Central
- Golgi disassembly Source: UniProtKB
- mitotic cell cycle Source: RGD
- mitotic cell cycle phase transition Source: RGD
- mitotic G2 DNA damage checkpoint Source: RGD
- negative regulation of apoptotic process Source: RGD
- peptidyl-serine phosphorylation Source: UniProtKB
- peptidyl-threonine phosphorylation Source: UniProtKB
- positive regulation of cardiac muscle cell proliferation Source: RGD
- positive regulation of DNA replication Source: RGD
- positive regulation of G2/M transition of mitotic cell cycle Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of mitochondrial ATP synthesis coupled electron transport Source: RGD
- positive regulation of mitotic cell cycle Source: RGD
- positive regulation of protein import into nucleus Source: RGD
- positive regulation of protein localization to nucleus Source: RGD
- protein-containing complex assembly Source: RGD
- protein localization to kinetochore Source: RGD
- protein phosphorylation Source: RGD
- regulation of circadian rhythm Source: UniProtKB
- response to activity Source: RGD
- response to amine Source: RGD
- response to axon injury Source: RGD
- response to cadmium ion Source: RGD
- response to copper ion Source: RGD
- response to drug Source: RGD
- response to ethanol Source: RGD
- response to hydrogen peroxide Source: RGD
- response to organic cyclic compound Source: RGD
- response to organonitrogen compound Source: RGD
- response to toxic substance Source: RGD
- rhythmic process Source: UniProtKB-KW
- ventricular cardiac muscle cell development Source: RGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Apoptosis, Biological rhythms, Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 5301 |
Reactomei | R-RNO-110056, MAPK3 (ERK1) activation R-RNO-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-176408, Regulation of APC/C activators between G1/S and early anaphase R-RNO-176412, Phosphorylation of the APC/C R-RNO-176417, Phosphorylation of Emi1 R-RNO-2299718, Condensation of Prophase Chromosomes R-RNO-2500257, Resolution of Sister Chromatid Cohesion R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-RNO-3301854, Nuclear Pore Complex (NPC) Disassembly R-RNO-380259, Loss of Nlp from mitotic centrosomes R-RNO-380270, Recruitment of mitotic centrosome proteins and complexes R-RNO-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-RNO-380320, Recruitment of NuMA to mitotic centrosomes R-RNO-4419969, Depolymerisation of the Nuclear Lamina R-RNO-5620912, Anchoring of the basal body to the plasma membrane R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689896, Ovarian tumor domain proteases R-RNO-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-RNO-6804757, Regulation of TP53 Degradation R-RNO-68875, Mitotic Prophase R-RNO-69273, Cyclin A/B1/B2 associated events during G2/M transition R-RNO-69478, G2/M DNA replication checkpoint R-RNO-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854518, AURKA Activation by TPX2 R-RNO-8878166, Transcriptional regulation by RUNX2 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Cdk1 Synonyms:Cdc2, Cdc2a, Cdkn1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2319, Cdk1 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
- spindle By similarity
Nucleus
- Nucleus 2 Publications
Mitochondrion
- Mitochondrion By similarity
Other locations
- Cytoplasm By similarity
Note: Cytoplasmic during the interphase. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle (By similarity).By similarity
Cytoskeleton
- centrosome Source: UniProtKB
- mitotic spindle Source: UniProtKB
- spindle microtubule Source: RGD
Cytosol
- cytosol Source: Ensembl
Mitochondrion
- mitochondrial matrix Source: Ensembl
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Other locations
- cyclin B1-CDK1 complex Source: RGD
- cyclin-dependent protein kinase holoenzyme complex Source: RGD
- cytoplasm Source: RGD
- midbody Source: RGD
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085727 | 1 – 297 | Cyclin-dependent kinase 1Add BLAST | 297 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 4 | Phosphotyrosine; by PKRBy similarity | 1 | |
Modified residuei | 6 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 6 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 9 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 9 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 14 | Phosphothreonine; by PKMYT1By similarity | 1 | |
Modified residuei | 15 | Phosphotyrosine; by PKMYT1, WEE1, WEE2 and PKC/PRKCDBy similarity | 1 | |
Modified residuei | 15 | Phosphotyrosine; by WEE1 and WEE2By similarity | 1 | |
Modified residuei | 19 | PhosphotyrosineBy similarity | 1 | |
Cross-linki | 20 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 39 | PhosphoserineBy similarity | 1 | |
Modified residuei | 77 | PhosphotyrosineBy similarity | 1 | |
Cross-linki | 139 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 141 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 161 | Phosphothreonine; by CAKBy similarity | 1 | |
Modified residuei | 178 | PhosphoserineBy similarity | 1 | |
Modified residuei | 222 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 245 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 248 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P39951 |
PaxDbi | P39951 |
PRIDEi | P39951 |
PTM databases
iPTMneti | P39951 |
PhosphoSitePlusi | P39951 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000000632, Expressed in thymus and 20 other tissues |
Genevisiblei | P39951, RN |
Interactioni
Subunit structurei
Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin.
Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Binds to RB1 and other transcription factors such as FOXO1 and RUNX2. Promotes G2-M transition when in complex with a cyclin-B.
Interacts with DLGAP5. Binds to the CDK inhibitors CDKN1A/p21 and CDKN1B/p27. Isoform 2 is unable to complex with cyclin-B1 and also fails to bind to CDKN1A/p21.
Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. Associates with cyclins-A and B1 during S-phase in regenerating hepatocytes.
Interacts with FANCC.
Interacts with CEP63; this interaction recruits CDK1 to centrosomes.
Interacts with CENPA.
Interacts with NR1D1 (By similarity).
Interacts with proteasome subunit PSMA8; to participate in meiosis progression during spermatogenesis (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- cyclin binding Source: RGD
- Hsp70 protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248460, 1 interactor |
ComplexPortali | CPX-2063, Cyclin A1-CDK1 complex CPX-2064, Cyclin A2-CDK1 complex CPX-2072, Cyclin B1-CDK1 complex |
CORUMi | P39951 |
IntActi | P39951, 2 interactors |
STRINGi | 10116.ENSRNOP00000000783 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 287 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 284 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000153335 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P39951 |
OMAi | MTHPYFD |
OrthoDBi | 1010560at2759 |
PhylomeDBi | P39951 |
TreeFami | TF101021 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEDYIKIEKI GEGTYGVVYK GRHRTTGQIV AMKKIRLESE EEGVPSTAIR
60 70 80 90 100
EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQFM
110 120 130 140 150
DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA
160 170 180 190 200
RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK
210 220 230 240 250
KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA
260 270 280 290
SHVKNLDENG LDLLSKMLVY DPAKRISGKM ALKHPYFDDL DNQIKKM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60767 mRNA Translation: CAA43177.1 BC091549 mRNA Translation: AAH91549.1 |
PIRi | S24913 |
RefSeqi | NP_062169.1, NM_019296.1 XP_006256415.1, XM_006256353.3 |
Genome annotation databases
Ensembli | ENSRNOT00000000783; ENSRNOP00000000783; ENSRNOG00000000632 ENSRNOT00000081113; ENSRNOP00000070138; ENSRNOG00000000632 ENSRNOT00000086806; ENSRNOP00000072365; ENSRNOG00000000632 |
GeneIDi | 54237 |
KEGGi | rno:54237 |
UCSCi | RGD:2319, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60767 mRNA Translation: CAA43177.1 BC091549 mRNA Translation: AAH91549.1 |
PIRi | S24913 |
RefSeqi | NP_062169.1, NM_019296.1 XP_006256415.1, XM_006256353.3 |
3D structure databases
SMRi | P39951 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248460, 1 interactor |
ComplexPortali | CPX-2063, Cyclin A1-CDK1 complex CPX-2064, Cyclin A2-CDK1 complex CPX-2072, Cyclin B1-CDK1 complex |
CORUMi | P39951 |
IntActi | P39951, 2 interactors |
STRINGi | 10116.ENSRNOP00000000783 |
PTM databases
iPTMneti | P39951 |
PhosphoSitePlusi | P39951 |
Proteomic databases
jPOSTi | P39951 |
PaxDbi | P39951 |
PRIDEi | P39951 |
Genome annotation databases
Ensembli | ENSRNOT00000000783; ENSRNOP00000000783; ENSRNOG00000000632 ENSRNOT00000081113; ENSRNOP00000070138; ENSRNOG00000000632 ENSRNOT00000086806; ENSRNOP00000072365; ENSRNOG00000000632 |
GeneIDi | 54237 |
KEGGi | rno:54237 |
UCSCi | RGD:2319, rat |
Organism-specific databases
CTDi | 983 |
RGDi | 2319, Cdk1 |
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000153335 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P39951 |
OMAi | MTHPYFD |
OrthoDBi | 1010560at2759 |
PhylomeDBi | P39951 |
TreeFami | TF101021 |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 5301 |
Reactomei | R-RNO-110056, MAPK3 (ERK1) activation R-RNO-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-RNO-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-RNO-176408, Regulation of APC/C activators between G1/S and early anaphase R-RNO-176412, Phosphorylation of the APC/C R-RNO-176417, Phosphorylation of Emi1 R-RNO-2299718, Condensation of Prophase Chromosomes R-RNO-2500257, Resolution of Sister Chromatid Cohesion R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-RNO-3301854, Nuclear Pore Complex (NPC) Disassembly R-RNO-380259, Loss of Nlp from mitotic centrosomes R-RNO-380270, Recruitment of mitotic centrosome proteins and complexes R-RNO-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-RNO-380320, Recruitment of NuMA to mitotic centrosomes R-RNO-4419969, Depolymerisation of the Nuclear Lamina R-RNO-5620912, Anchoring of the basal body to the plasma membrane R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-5689896, Ovarian tumor domain proteases R-RNO-6804114, TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest R-RNO-6804757, Regulation of TP53 Degradation R-RNO-68875, Mitotic Prophase R-RNO-69273, Cyclin A/B1/B2 associated events during G2/M transition R-RNO-69478, G2/M DNA replication checkpoint R-RNO-75035, Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex R-RNO-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-RNO-8854518, AURKA Activation by TPX2 R-RNO-8878166, Transcriptional regulation by RUNX2 |
Miscellaneous databases
PROi | PR:P39951 |
Gene expression databases
Bgeei | ENSRNOG00000000632, Expressed in thymus and 20 other tissues |
Genevisiblei | P39951, RN |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK1_RAT | |
Accessioni | P39951Primary (citable) accession number: P39951 Secondary accession number(s): Q5BJB4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families