UniProtKB - P39946 (LIS1_YEAST)
Protein
Nuclear distribution protein PAC1
Gene
PAC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck. Following plus end binding, dynein may be offloaded to the cortex by NUM1. Required for viability in the absence of the kinesin-related microtubule-dependent motor protein CIN8.UniRule annotation3 Publications
GO - Molecular functioni
- dynein complex binding Source: GO_Central
- identical protein binding Source: IntAct
- microtubule plus-end binding Source: SGD
GO - Biological processi
- cell division Source: UniProtKB-KW
- establishment of mitotic spindle orientation Source: GO_Central
- microtubule organizing center organization Source: GO_Central
- microtubule sliding Source: UniProtKB-UniRule
- nuclear migration Source: GO_Central
- nuclear migration along microtubule Source: SGD
- positive regulation of ATP-dependent microtubule motor activity, plus-end-directed Source: SGD
- positive regulation of microtubule plus-end binding Source: SGD
- regulation of microtubule motor activity Source: GO_Central
- vesicle transport along microtubule Source: GO_Central
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-8951664, Neddylation R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear distribution protein PAC1UniRule annotationAlternative name(s): Lissencephaly-1 homologUniRule annotation Short name: LIS-1UniRule annotation nudF homologUniRule annotation |
Gene namesi | Ordered Locus Names:YOR269W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR269W |
SGDi | S000005795, PAC1 |
Subcellular locationi
Cytoskeleton
Nucleus
Note: Localizes to the plus ends of cytoplasmic microtubules.
Cytoskeleton
- cytoplasmic microtubule Source: SGD
- microtubule associated complex Source: GO_Central
- spindle pole Source: UniProtKB-SubCell
Nucleus
- nuclear envelope Source: GO_Central
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- kinetochore Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051113 | 1 – 494 | Nuclear distribution protein PAC1Add BLAST | 494 |
Proteomic databases
MaxQBi | P39946 |
PaxDbi | P39946 |
PRIDEi | P39946 |
Interactioni
Subunit structurei
Self-associates.
Interacts with NDL1. May form a complex with dynein.
1 PublicationBinary interactionsi
Hide detailsP39946
With | #Exp. | IntAct |
---|---|---|
KEL1 [P38853] | 3 | EBI-12838,EBI-9619 |
NNF1 [P47149] | 3 | EBI-12838,EBI-12098 |
itself | 3 | EBI-12838,EBI-12838 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- microtubule plus-end binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 34658, 366 interactors |
DIPi | DIP-1485N |
IntActi | P39946, 14 interactors |
MINTi | P39946 |
STRINGi | 4932.YOR269W |
Miscellaneous databases
RNActi | P39946, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 46 | LisHUniRule annotationAdd BLAST | 33 | |
Repeati | 153 – 192 | WD 1Add BLAST | 40 | |
Repeati | 196 – 244 | WD 2Add BLAST | 49 | |
Repeati | 251 – 292 | WD 3Add BLAST | 42 | |
Repeati | 295 – 334 | WD 4Add BLAST | 40 | |
Repeati | 347 – 395 | WD 5Add BLAST | 49 | |
Repeati | 415 – 454 | WD 6Add BLAST | 40 | |
Repeati | 457 – 492 | WD 7Add BLAST | 36 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 90 – 123 | UniRule annotationAdd BLAST | 34 |
Domaini
Dimerization mediated by the LisH domain may be required to activate dynein.UniRule annotation
Sequence similaritiesi
Belongs to the WD repeat LIS1/nudF family.UniRule annotation
Keywords - Domaini
Coiled coil, Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0295, Eukaryota |
GeneTreei | ENSGT00940000155039 |
HOGENOMi | CLU_000288_57_15_1 |
InParanoidi | P39946 |
OMAi | PERYAMS |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03141, lis1, 1 hit |
InterProi | View protein in InterPro IPR017252, Dynein_regulator_LIS1 IPR020472, G-protein_beta_WD-40_rep IPR037190, LIS1_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PIRSFi | PIRSF037647, Dynein_regulator_Lis1, 1 hit |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF109925, SSF109925, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 2 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P39946-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNWQQQLPL TDTQKNELDK SVLRYLNWNY KQTVRHEHAQ DYESVRHAIV
60 70 80 90 100
TLSGFLLQES VDRQEFISNN DTSNESMVDI DELLLPKKWN SIVRLQKKII
110 120 130 140 150
ELEQNTETLV SQIKDLNTQV SELAQFKPTT SNGTSAHNVL KWIPRNLPSC
160 170 180 190 200
LINVESSVTS VKLHPNLPIV FVATDHGKLY AFDLFNYTIP LASLQSHTKA
210 220 230 240 250
ITSMDVLFTN YTNSSKKNYL VIVTASKDLQ IHVFKWVSEE CKFQQIRSLL
260 270 280 290 300
GHEHIVSAVK IWQKNNDVHI ASCSRDQTVK IWDFHNGWSL KTFQPHSQWV
310 320 330 340 350
RSIDVLGDYI ISGSHDTTLR LTHWPSGNGL SVGTGHEFPI EKVKFIHFIE
360 370 380 390 400
DSPEIRFRTP STDRYKNWGM QYCVSASRDR TIKIWEIPLP TLMAHRAPIP
410 420 430 440 450
NPTDSNFRCV LTLKGHLSWV RDISIRGQYL FSCADDKSVR CWDLNTGQCL
460 470 480 490
HVWEKLHTGF VNCLDLDVDF DSNVTPRQMM VTGGLDCKSN VFMR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16827 Genomic DNA Translation: AAB00685.1 X89633 Genomic DNA Translation: CAA61775.1 Z75177 Genomic DNA Translation: CAA99493.1 BK006948 Genomic DNA Translation: DAA11035.1 |
PIRi | S67166 |
RefSeqi | NP_014912.1, NM_001183688.1 |
Genome annotation databases
EnsemblFungii | YOR269W_mRNA; YOR269W; YOR269W |
GeneIDi | 854443 |
KEGGi | sce:YOR269W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U16827 Genomic DNA Translation: AAB00685.1 X89633 Genomic DNA Translation: CAA61775.1 Z75177 Genomic DNA Translation: CAA99493.1 BK006948 Genomic DNA Translation: DAA11035.1 |
PIRi | S67166 |
RefSeqi | NP_014912.1, NM_001183688.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5VH9 | electron microscopy | 7.70 | B | 140-493 | [»] | |
5VLJ | electron microscopy | 10.50 | B/C | 140-493 | [»] | |
SMRi | P39946 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34658, 366 interactors |
DIPi | DIP-1485N |
IntActi | P39946, 14 interactors |
MINTi | P39946 |
STRINGi | 4932.YOR269W |
Proteomic databases
MaxQBi | P39946 |
PaxDbi | P39946 |
PRIDEi | P39946 |
Genome annotation databases
EnsemblFungii | YOR269W_mRNA; YOR269W; YOR269W |
GeneIDi | 854443 |
KEGGi | sce:YOR269W |
Organism-specific databases
EuPathDBi | FungiDB:YOR269W |
SGDi | S000005795, PAC1 |
Phylogenomic databases
eggNOGi | KOG0295, Eukaryota |
GeneTreei | ENSGT00940000155039 |
HOGENOMi | CLU_000288_57_15_1 |
InParanoidi | P39946 |
OMAi | PERYAMS |
Enzyme and pathway databases
Reactomei | R-SCE-8951664, Neddylation R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P39946 |
RNActi | P39946, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03141, lis1, 1 hit |
InterProi | View protein in InterPro IPR017252, Dynein_regulator_LIS1 IPR020472, G-protein_beta_WD-40_rep IPR037190, LIS1_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PIRSFi | PIRSF037647, Dynein_regulator_Lis1, 1 hit |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF109925, SSF109925, 1 hit SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 2 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LIS1_YEAST | |
Accessioni | P39946Primary (citable) accession number: P39946 Secondary accession number(s): D6W2W9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD