UniProtKB - P39940 (RSP5_YEAST)
E3 ubiquitin-protein ligase RSP5
RSP5
Functioni
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner.
12 PublicationsMiscellaneous
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 777 | Glycyl thioester intermediate | 1 |
GO - Molecular functioni
- phosphatidylinositol binding Source: SGD
- ubiquitin binding Source: SGD
- ubiquitin protein ligase activity Source: MGI
- ubiquitin-protein transferase activity Source: SGD
GO - Biological processi
- cellular response to UV Source: SGD
- chromatin assembly or disassembly Source: SGD
- late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
- mitochondria-associated ubiquitin-dependent protein catabolic process Source: SGD
- mitochondrion organization Source: SGD
- poly(A)+ mRNA export from nucleus Source: SGD
- positive regulation of endocytosis Source: SGD
- positive regulation of fatty acid biosynthetic process Source: SGD
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: SGD
- positive regulation of protein catabolic process Source: GO_Central
- positive regulation of receptor-mediated endocytosis Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
- protein autoubiquitination Source: SGD
- protein monoubiquitination Source: SGD
- protein polyubiquitination Source: SGD
- protein ubiquitination Source: SGD
- regulation of actin cytoskeleton organization Source: SGD
- regulation of dolichol biosynthetic process Source: SGD
- regulation of ergosterol biosynthetic process Source: SGD
- regulation of initiation of mating projection growth Source: SGD
- regulation of mRNA export from nucleus Source: SGD
- regulation of multivesicular body size Source: SGD
- regulation of nitrogen utilization Source: SGD
- regulation of phosphate metabolic process Source: SGD
- regulation of protein localization Source: SGD
- regulation of ribosomal large subunit export from nucleus Source: SGD
- regulation of rRNA processing Source: SGD
- regulation of tRNA export from nucleus Source: SGD
- regulation of tRNA processing Source: SGD
- regulation of ubiquinone biosynthetic process Source: SGD
- response to xenobiotic stimulus Source: SGD
- ribophagy Source: SGD
- ubiquitin-dependent endocytosis Source: SGD
- ubiquitin-dependent protein catabolic process Source: MGI
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
BRENDAi | 2.3.2.26, 984 |
Reactomei | R-SCE-8948751, Regulation of PTEN stability and activity R-SCE-9013420, RHOU GTPase cycle R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family 8.A.30.1.4, the nedd4-family interacting protein-2 (nedd4) family |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RSP5 (EC:2.3.2.26)Alternative name(s): HECT-type E3 ubiquitin transferase RSP5 Reverses SPT-phenotype protein 5 |
Gene namesi | Name:RSP5 Synonyms:MDP1, NPI1 Ordered Locus Names:YER125W ORF Names:SYGP-ORF41 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000927, RSP5 |
VEuPathDBi | FungiDB:YER125W |
Subcellular locationi
Nucleus
- Nucleus Curated
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- actin patch 1 Publication
Cytoskeleton
- actin cortical patch Source: UniProtKB-SubCell
Endosome
- endosome membrane Source: SGD
Golgi apparatus
- Golgi apparatus Source: SGD
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: SGD
- plasma membrane Source: SGD
Other locations
- cellular bud tip Source: SGD
- cytoplasm Source: SGD
- RSP5-BUL ubiquitin ligase complex Source: ComplexPortal
- ubiquitin ligase complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 516 | Y → A: Has subtle defects on both initial ubiquitination and chain elongation of substrate proteins. | 1 | |
Mutagenesisi | 521 | Y → A: Has defects on both initial ubiquitination and chain elongation of substrate proteins. | 1 | |
Mutagenesisi | 537 | I → D: Has defects on both initial ubiquitination and chain elongation of substrate proteins. | 1 | |
Mutagenesisi | 618 | F → D: Has defects on both initial ubiquitination and chain elongation of substrate proteins. | 1 | |
Mutagenesisi | 733 | L → S in RSP5-1; impairs ubiquitin-thioester formation and catalysis of substrate ubiquitination. 1 Publication | 1 | |
Mutagenesisi | 777 | C → A: Loss of ubiquitination. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120335 | 1 – 809 | E3 ubiquitin-protein ligase RSP5Add BLAST | 809 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 258 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P39940 |
PaxDbi | P39940 |
PRIDEi | P39940 |
PTM databases
iPTMneti | P39940 |
Interactioni
Subunit structurei
Component of the RSP5-BUL1/2 ubiquitin ligase complex composed of at least RSP5 and BUL1 or BUL2.
Forms also a ternary complex with RUP1 and UBP2.
Interacts (via WW domains) with LSB1, PIN3/LSB2 and RCR1 (via PY motifs).
Interacts with HSE1, LAS17, ROG3, ROD1 and RVS167.
9 PublicationsBinary interactionsi
P39940
GO - Molecular functioni
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 36869, 1260 interactors |
ComplexPortali | CPX-2921, RSP5-BUL1 ubiquitin ligase complex CPX-2923, RSP5-BUL2 ubiquitin ligase complex |
DIPi | DIP-2238N |
ELMi | P39940 |
IntActi | P39940, 189 interactors |
MINTi | P39940 |
STRINGi | 4932.YER125W |
Miscellaneous databases
RNActi | P39940, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P39940 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P39940 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 105 | C2PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 229 – 262 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 331 – 364 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 387 – 420 | WW 3PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 705 – 809 | HECTPROSITE-ProRule annotationAdd BLAST | 105 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 99 – 122 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 139 – 240 | DisorderedSequence analysisAdd BLAST | 102 | |
Regioni | 257 – 298 | DisorderedSequence analysisAdd BLAST | 42 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 103 – 119 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 139 – 225 | Polar residuesSequence analysisAdd BLAST | 87 | |
Compositional biasi | 257 – 277 | Polar residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0940, Eukaryota |
HOGENOMi | CLU_002173_0_0_1 |
InParanoidi | P39940 |
OMAi | RAHQSRM |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit cd00201, WW, 3 hits |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR024928, E3_ub_ligase_SMURF1 IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF00632, HECT, 1 hit PF00397, WW, 3 hits |
PIRSFi | PIRSF001569, E3_ub_ligase_SMURF1, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00119, HECTc, 1 hit SM00456, WW, 3 hits |
SUPFAMi | SSF49562, SSF49562, 1 hit SSF51045, SSF51045, 3 hits SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS50237, HECT, 1 hit PS01159, WW_DOMAIN_1, 3 hits PS50020, WW_DOMAIN_2, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPSSISVKLV AAESLYKRDV FRSPDPFAVL TIDGYQTKST SAAKKTLNPY
60 70 80 90 100
WNETFKFDDI NENSILTIQV FDQKKFKKKD QGFLGVVNVR VGDVLGHLDE
110 120 130 140 150
DTATSSGRPR EETITRDLKK SNDGMAVSGR LIVVLSKLPS SSPHSQAPSG
160 170 180 190 200
HTASSSTNTS STTRTNGHST SSTRNHSTSH PSRGTAQAVE STLQSGTTAA
210 220 230 240 250
TNTATTSHRS TNSTSSATRQ YSSFEDQYGR LPPGWERRTD NFGRTYYVDH
260 270 280 290 300
NTRTTTWKRP TLDQTEAERG NQLNANTELE RRQHRGRTLP GGSSDNSSVT
310 320 330 340 350
VQVGGGSNIP PVNGAAAAAF AATGGTTSGL GELPSGWEQR FTPEGRAYFV
360 370 380 390 400
DHNTRTTTWV DPRRQQYIRT YGPTNTTIQQ QPVSQLGPLP SGWEMRLTNT
410 420 430 440 450
ARVYFVDHNT KTTTWDDPRL PSSLDQNVPQ YKRDFRRKVI YFRSQPALRI
460 470 480 490 500
LPGQCHIKVR RKNIFEDAYQ EIMRQTPEDL KKRLMIKFDG EEGLDYGGVS
510 520 530 540 550
REFFFLLSHE MFNPFYCLFE YSAYDNYTIQ INPNSGINPE HLNYFKFIGR
560 570 580 590 600
VVGLGVFHRR FLDAFFVGAL YKMMLRKKVV LQDMEGVDAE VYNSLNWMLE
610 620 630 640 650
NSIDGVLDLT FSADDERFGE VVTVDLKPDG RNIEVTDGNK KEYVELYTQW
660 670 680 690 700
RIVDRVQEQF KAFMDGFNEL IPEDLVTVFD ERELELLIGG IAEIDIEDWK
710 720 730 740 750
KHTDYRGYQE SDEVIQWFWK CVSEWDNEQR ARLLQFTTGT SRIPVNGFKD
760 770 780 790 800
LQGSDGPRRF TIEKAGEVQQ LPKSHTCFNR VDLPQYVDYD SMKQKLTLAV
EETIGFGQE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18916 Genomic DNA Translation: AAC03223.1 BK006939 Genomic DNA Translation: DAA07785.1 |
PIRi | S43217 |
RefSeqi | NP_011051.3, NM_001179015.3 |
Genome annotation databases
EnsemblFungii | YER125W_mRNA; YER125W; YER125W |
GeneIDi | 856862 |
KEGGi | sce:YER125W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18916 Genomic DNA Translation: AAC03223.1 BK006939 Genomic DNA Translation: DAA07785.1 |
PIRi | S43217 |
RefSeqi | NP_011051.3, NM_001179015.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3OLM | X-ray | 2.50 | A | 384-809 | [»] | |
4LCD | X-ray | 3.10 | A/B | 383-809 | [»] | |
5HPL | X-ray | 2.31 | A/B | 430-809 | [»] | |
SMRi | P39940 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36869, 1260 interactors |
ComplexPortali | CPX-2921, RSP5-BUL1 ubiquitin ligase complex CPX-2923, RSP5-BUL2 ubiquitin ligase complex |
DIPi | DIP-2238N |
ELMi | P39940 |
IntActi | P39940, 189 interactors |
MINTi | P39940 |
STRINGi | 4932.YER125W |
Protein family/group databases
TCDBi | 3.A.31.1.1, the endosomal sorting complexes required for transport iii (escrt-iii) family 8.A.30.1.4, the nedd4-family interacting protein-2 (nedd4) family |
PTM databases
iPTMneti | P39940 |
Proteomic databases
MaxQBi | P39940 |
PaxDbi | P39940 |
PRIDEi | P39940 |
Genome annotation databases
EnsemblFungii | YER125W_mRNA; YER125W; YER125W |
GeneIDi | 856862 |
KEGGi | sce:YER125W |
Organism-specific databases
SGDi | S000000927, RSP5 |
VEuPathDBi | FungiDB:YER125W |
Phylogenomic databases
eggNOGi | KOG0940, Eukaryota |
HOGENOMi | CLU_002173_0_0_1 |
InParanoidi | P39940 |
OMAi | RAHQSRM |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.26, 984 |
Reactomei | R-SCE-8948751, Regulation of PTEN stability and activity R-SCE-9013420, RHOU GTPase cycle R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
EvolutionaryTracei | P39940 |
PROi | PR:P39940 |
RNActi | P39940, protein |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit cd00201, WW, 3 hits |
Gene3Di | 2.60.40.150, 1 hit |
InterProi | View protein in InterPro IPR000008, C2_dom IPR035892, C2_domain_sf IPR024928, E3_ub_ligase_SMURF1 IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase IPR001202, WW_dom IPR036020, WW_dom_sf |
Pfami | View protein in Pfam PF00168, C2, 1 hit PF00632, HECT, 1 hit PF00397, WW, 3 hits |
PIRSFi | PIRSF001569, E3_ub_ligase_SMURF1, 1 hit |
SMARTi | View protein in SMART SM00239, C2, 1 hit SM00119, HECTc, 1 hit SM00456, WW, 3 hits |
SUPFAMi | SSF49562, SSF49562, 1 hit SSF51045, SSF51045, 3 hits SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50004, C2, 1 hit PS50237, HECT, 1 hit PS01159, WW_DOMAIN_1, 3 hits PS50020, WW_DOMAIN_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | RSP5_YEAST | |
Accessioni | P39940Primary (citable) accession number: P39940 Secondary accession number(s): D3DM31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 214 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families