UniProtKB - P39936 (IF4F2_YEAST)
Protein
Eukaryotic initiation factor 4F subunit p130
Gene
TIF4632
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'-ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4632 is probably essential when TIF4631 is missing.2 Publications
Miscellaneous
Present with 3390 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- ATPase activator activity Source: SGD
- mRNA binding Source: SGD
- translation initiation factor activity Source: SGD
- translation initiation factor binding Source: SGD
GO - Biological processi
- positive regulation of formation of translation preinitiation complex Source: SGD
- regulation of RNA helicase activity Source: SGD
- stress granule assembly Source: SGD
- translational initiation Source: SGD
Keywordsi
Molecular function | Initiation factor, RNA-binding |
Biological process | Protein biosynthesis, Translation regulation |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-166208, mTORC1-mediated signalling R-SCE-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-SCE-72702, Ribosomal scanning and start codon recognition R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic initiation factor 4F subunit p130Short name: eIF-4F p130 Short name: eIF4F p130 Alternative name(s): eIF4G2 mRNA cap-binding protein complex subunit p130 |
Gene namesi | Name:TIF4632 Ordered Locus Names:YGL049C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003017, TIF4632 |
VEuPathDBi | FungiDB:YGL049C |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Other locations
- cytoplasmic stress granule Source: SGD
- eukaryotic translation initiation factor 4F complex Source: SGD
- P-body Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 233 – 236 | RLRK → AVAA in TIF4632-233; abolishes interaction with PAB1 and inhibits poly(A)-dependent translation. 1 Publication | 4 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213332 | 1 – 914 | Eukaryotic initiation factor 4F subunit p130Add BLAST | 914 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 74 | PhosphoserineCombined sources | 1 | |
Modified residuei | 196 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 301 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 503 | PhosphoserineCombined sources | 1 | |
Modified residuei | 913 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P39936 |
PaxDbi | P39936 |
PRIDEi | P39936 |
PTM databases
iPTMneti | P39936 |
Interactioni
Subunit structurei
Component of the eIF4F complex, which composition varies with external and internal environmental conditions. It is composed of at least eIF4A (TIF1/TIF2), eIF4E (TIF45) and eIF4G (TIF4631 or TIF4632) (By similarity).
Interacts with PAT1 in a RNA-dependent manner.
By similarity5 PublicationsBinary interactionsi
P39936
With | #Exp. | IntAct |
---|---|---|
PAB1 [P04147] | 2 | EBI-9006,EBI-12823 |
GO - Molecular functioni
- translation initiation factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 33199, 115 interactors |
ComplexPortali | CPX-431, Eukaryotic translation initiation factor 4F complex, variant TIF4632 |
DIPi | DIP-330N |
ELMi | P39936 |
IntActi | P39936, 34 interactors |
MINTi | P39936 |
STRINGi | 4932.YGL049C |
Miscellaneous databases
RNActi | P39936, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 567 – 810 | MIF4GAdd BLAST | 244 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 201 – 315 | Interaction with PAB1Add BLAST | 115 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 32 – 97 | Asn-richAdd BLAST | 66 | |
Compositional biasi | 459 – 510 | Arg/Ser-richAdd BLAST | 52 | |
Compositional biasi | 840 – 863 | Arg/Ser-richAdd BLAST | 24 |
Sequence similaritiesi
Belongs to the eukaryotic initiation factor 4G family.Curated
Phylogenomic databases
eggNOGi | KOG0401, Eukaryota |
GeneTreei | ENSGT00940000154675 |
HOGENOMi | CLU_006715_1_0_1 |
InParanoidi | P39936 |
OMAi | VEMELWE |
Family and domain databases
Gene3Di | 1.20.970.30, 1 hit 1.25.40.180, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR022745, eIF4G1_eIF4E-bd IPR036211, eIF4G_eIF4E-bd_sf IPR016021, MIF4-like_sf IPR003890, MIF4G-like_typ-3 |
Pfami | View protein in Pfam PF12152, eIF_4G1, 1 hit PF02854, MIF4G, 1 hit |
SMARTi | View protein in SMART SM00543, MIF4G, 1 hit |
SUPFAMi | SSF101489, SSF101489, 1 hit SSF48371, SSF48371, 1 hit |
i Sequence
Sequence statusi: Complete.
P39936-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDQRGPPPP HPQQANGYKK FPPHDNQYSG ANNSQPNNHY NENLYSAREP
60 70 80 90 100
HNNKQYQSKN GKYGTNKYNN RNNSQGNAQY YNNRFNNGYR LNNNDYNPAM
110 120 130 140 150
LPGMQWPANY YAPQMYYIPQ QMVPVASPPY THQPLNTNPE PPSTPKTTKI
160 170 180 190 200
EITTKTGERL NLKKFHEEKK ASKGEEKNDG VEQKSKSGTP FEKEATPVLP
210 220 230 240 250
ANEAVKDTLT ETSNEKSTSE AENTKRLFLE QVRLRKAAME RKKNGLISET
260 270 280 290 300
EKKQETSNHD NTDTTKPNSV IESEPIKEAP KPTGEANEVV IDGKSGASVK
310 320 330 340 350
TPQHVTGSVT KSVTFNEPEN ESSSQDVDEL VKDDDTTEIS DTTGGKTVNK
360 370 380 390 400
SDDETINSVI TTEENTVKET EPSTSDIEMP TVSQLLETLG KAQPISDIYE
410 420 430 440 450
FAYPENVERP DIKYKKPSVK YTYGPTFLLQ FKDKLKFRPD PAWVEAVSSK
460 470 480 490 500
IVIPPHIARN KPKDSGRFGG DFRSPSMRGM DHTSSSRVSS KRRSKRMGDD
510 520 530 540 550
RRSNRGYTSR KDREKAAEKA EEQAPKEEIA PLVPSANRWI PKSRVKKTEK
560 570 580 590 600
KLAPDGKTEL FDKEEVERKM KSLLNKLTLE MFDSISSEIL DIANQSKWED
610 620 630 640 650
DGETLKIVIE QIFHKACDEP HWSSMYAQLC GKVVKDLDPN IKDKENEGKN
660 670 680 690 700
GPKLVLHYLV ARCHEEFEKG WADKLPAGED GNPLEPEMMS DEYYIAAAAK
710 720 730 740 750
RRGLGLVRFI GYLYCLNLLT GKMMFECFRR LMKDLNNDPS EETLESVIEL
760 770 780 790 800
LNTVGEQFEH DKFVTPQATL EGSVLLDNLF MLLQHIIDGG TISNRIKFKL
810 820 830 840 850
IDVKELREIK HWNSAKKDAG PKTIQQIHQE EEQLRQKKNS QRSNSRFNNH
860 870 880 890 900
NQSNSNRYSS NRRNMQNTQR DSFASTKTGS FRNNQRNARK VEEVSQAPRA
910
NMFDALMNND GDSD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L16924 Unassigned DNA Translation: AAA18474.1 Z72571 Genomic DNA Translation: CAA96751.1 BK006941 Genomic DNA Translation: DAA08052.1 |
PIRi | B48086 |
RefSeqi | NP_011466.1, NM_001180914.1 |
Genome annotation databases
EnsemblFungii | YGL049C_mRNA; YGL049C; YGL049C |
GeneIDi | 852833 |
KEGGi | sce:YGL049C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L16924 Unassigned DNA Translation: AAA18474.1 Z72571 Genomic DNA Translation: CAA96751.1 BK006941 Genomic DNA Translation: DAA08052.1 |
PIRi | B48086 |
RefSeqi | NP_011466.1, NM_001180914.1 |
3D structure databases
SMRi | P39936 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33199, 115 interactors |
ComplexPortali | CPX-431, Eukaryotic translation initiation factor 4F complex, variant TIF4632 |
DIPi | DIP-330N |
ELMi | P39936 |
IntActi | P39936, 34 interactors |
MINTi | P39936 |
STRINGi | 4932.YGL049C |
PTM databases
iPTMneti | P39936 |
Proteomic databases
MaxQBi | P39936 |
PaxDbi | P39936 |
PRIDEi | P39936 |
Genome annotation databases
EnsemblFungii | YGL049C_mRNA; YGL049C; YGL049C |
GeneIDi | 852833 |
KEGGi | sce:YGL049C |
Organism-specific databases
SGDi | S000003017, TIF4632 |
VEuPathDBi | FungiDB:YGL049C |
Phylogenomic databases
eggNOGi | KOG0401, Eukaryota |
GeneTreei | ENSGT00940000154675 |
HOGENOMi | CLU_006715_1_0_1 |
InParanoidi | P39936 |
OMAi | VEMELWE |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-166208, mTORC1-mediated signalling R-SCE-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-SCE-72702, Ribosomal scanning and start codon recognition R-SCE-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-SCE-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
PROi | PR:P39936 |
RNActi | P39936, protein |
Family and domain databases
Gene3Di | 1.20.970.30, 1 hit 1.25.40.180, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR022745, eIF4G1_eIF4E-bd IPR036211, eIF4G_eIF4E-bd_sf IPR016021, MIF4-like_sf IPR003890, MIF4G-like_typ-3 |
Pfami | View protein in Pfam PF12152, eIF_4G1, 1 hit PF02854, MIF4G, 1 hit |
SMARTi | View protein in SMART SM00543, MIF4G, 1 hit |
SUPFAMi | SSF101489, SSF101489, 1 hit SSF48371, SSF48371, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IF4F2_YEAST | |
Accessioni | P39936Primary (citable) accession number: P39936 Secondary accession number(s): D6VU91 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families