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Entry version 142 (02 Jun 2021)
Sequence version 1 (01 Feb 1995)
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Protein

Neutral protease B

Gene

nprB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Protease able to cleave casein in vitro.

1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Protease activity can be inhibited in vitro by either a zinc specific chelator, 1,10-phenanthroline, or a metal chelator, EDTA. The enzyme is resistant to phenylmethylsulfonyl fluoride and iodoacetic acid.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Optimum pH is 6.6.1 Publication

Temperature dependencei

Retains 65% activity after treatment at 65 degrees Celsius for 20 minutes.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi365Calcium 1Sequence analysis1
Metal bindingi369Zinc; catalyticPROSITE-ProRule annotation1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei370PROSITE-ProRule annotation1
Metal bindingi373Zinc; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi393Zinc; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi404Calcium 1Sequence analysis1
Metal bindingi404Calcium 2Sequence analysis1
Metal bindingi406Calcium 2; via carbonyl oxygenSequence analysis1
Metal bindingi407Calcium 1Sequence analysis1
Metal bindingi407Calcium 2Sequence analysis1
Metal bindingi409Calcium 1; via carbonyl oxygenSequence analysis1
Metal bindingi412Calcium 1Sequence analysis1
Metal bindingi412Calcium 2Sequence analysis1
Metal bindingi415Calcium 3; via carbonyl oxygenSequence analysis1
Metal bindingi416Calcium 3Sequence analysis1
Metal bindingi419Calcium 3; via carbonyl oxygenSequence analysis1
Metal bindingi422Calcium 3Sequence analysis1
Active sitei453Proton donorPROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU11100-MONOMER

Protein family/group databases

MEROPS protease database

More...
MEROPSi
M04.012

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Neutral protease B1 Publication (EC:3.4.24.-1 Publication)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nprB1 Publication
Ordered Locus Names:BSU11100
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Secreted

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene grow normally in both LB and SG media, and no difference in sporulation frequency can be observed.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 28Sequence analysisAdd BLAST28
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes a propeptide, which is a part of a protein that is cleaved during maturation or activation. Once cleaved, a propeptide generally has no independent biological function.<p><a href='/help/propep' target='_top'>More...</a></p>PropeptideiPRO_000002860829 – 223Activation peptide1 PublicationAdd BLAST195
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_0000028609224 – 538Neutral protease BAdd BLAST315

Keywords - PTMi

Zymogen

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P39899

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU11100

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P39899

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni421 – 441DisorderedSequence analysisAdd BLAST21

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG3227, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P39899

Identification of Orthologs from Complete Genome Data

More...
OMAi
TFDAENM

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P39899

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.390.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011096, FTP_domain
IPR025711, PepSY
IPR023612, Peptidase_M4
IPR027268, Peptidase_M4/M1_CTD_sf
IPR001570, Peptidase_M4_C_domain
IPR013856, Peptidase_M4_domain

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07504, FTP, 1 hit
PF03413, PepSY, 1 hit
PF01447, Peptidase_M4, 1 hit
PF02868, Peptidase_M4_C, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00730, THERMOLYSIN

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00142, ZINC_PROTEASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P39899-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRNLTKTSLL LAGLCTAAQM VFVTHASAEE SIEYDHTYQT PSYIIEKSPQ
60 70 80 90 100
KPVQNTTQKE SLFSYLDKHQ TQFKLKGNAN SHFRVSKTIK DPKTKQTFFK
110 120 130 140 150
LTEVYKGIPI YGFEQAVAMK ENKQVKSFFG KVHPQIKDVS VTPSISEKKA
160 170 180 190 200
IHTARRELEA SIGKIEYLDG EPKGELYIYP HDGEYDLAYL VRLSTSEPEP
210 220 230 240 250
GYWHYFIDAK NGKVIESFNA IHEAAGTGIG VSGDEKSFDV TEQNGRFYLA
260 270 280 290 300
DETRGKGINT FDAKNLNETL FTLLSQLIGY TGKEIVSGTS VFNEPAAVDA
310 320 330 340 350
HANAQAVYDY YSKTFGRDSF DQNGARITST VHVGKQWNNA AWNGVQMVYG
360 370 380 390 400
DGDGSKFKPL SGSLDIVAHE ITHAVTQYSA GLLYQGEPGA LNESISDIMG
410 420 430 440 450
AMADRDDWEI GEDVYTPGIA GDSLRSLEDP SKQGNPDHYS NRYTGTEDYG
460 470 480 490 500
GVHINSSIHN KAAYLLAEGG VHHGVQVEGI GREASEQIYY RALTYYVTAS
510 520 530
TDFSMMKQAA IEAANDLYGE GSKQSASVEK AYEAVGIL
Length:538
Mass (Da):59,336
Last modified:February 1, 1995 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4B7D2B8D8F64F14E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M62845 Genomic DNA Translation: AAA22626.1
Z79580 Genomic DNA Translation: CAB01832.1
Y09476 Genomic DNA Translation: CAA70628.1
AL009126 Genomic DNA Translation: CAB12950.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A41042

NCBI Reference Sequences

More...
RefSeqi
NP_388991.1, NC_000964.3
WP_009966979.1, NZ_JNCM01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB12950; CAB12950; BSU_11100

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
936384

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU11100

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.1192

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62845 Genomic DNA Translation: AAA22626.1
Z79580 Genomic DNA Translation: CAB01832.1
Y09476 Genomic DNA Translation: CAA70628.1
AL009126 Genomic DNA Translation: CAB12950.1
PIRiA41042
RefSeqiNP_388991.1, NC_000964.3
WP_009966979.1, NZ_JNCM01000035.1

3D structure databases

SMRiP39899
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU11100

Protein family/group databases

MEROPSiM04.012

Proteomic databases

PaxDbiP39899

Genome annotation databases

EnsemblBacteriaiCAB12950; CAB12950; BSU_11100
GeneIDi936384
KEGGibsu:BSU11100
PATRICifig|224308.179.peg.1192

Phylogenomic databases

eggNOGiCOG3227, Bacteria
InParanoidiP39899
OMAiTFDAENM
PhylomeDBiP39899

Enzyme and pathway databases

BioCyciBSUB:BSU11100-MONOMER

Family and domain databases

Gene3Di1.10.390.10, 1 hit
InterProiView protein in InterPro
IPR011096, FTP_domain
IPR025711, PepSY
IPR023612, Peptidase_M4
IPR027268, Peptidase_M4/M1_CTD_sf
IPR001570, Peptidase_M4_C_domain
IPR013856, Peptidase_M4_domain
PfamiView protein in Pfam
PF07504, FTP, 1 hit
PF03413, PepSY, 1 hit
PF01447, Peptidase_M4, 1 hit
PF02868, Peptidase_M4_C, 1 hit
PRINTSiPR00730, THERMOLYSIN
PROSITEiView protein in PROSITE
PS00142, ZINC_PROTEASE, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNPRB_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P39899
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 2, 2021
This is version 142 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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