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Protein

Metalloproteinase inhibitor 3

Gene

Timp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partnerBy similarity1
Sitei37Involved in metalloproteinase-bindingBy similarity1

GO - Molecular functioni

  • metalloendopeptidase inhibitor activity Source: GO_Central
  • protease binding Source: GO_Central
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionMetalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Protein family/group databases

MEROPSiI35.003

Names & Taxonomyi

Protein namesi
Recommended name:
Metalloproteinase inhibitor 3
Alternative name(s):
Tissue inhibitor of metalloproteinases 3
Short name:
TIMP-3
Gene namesi
Name:Timp3
Synonyms:Sun, Timp-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:98754 Timp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000003434424 – 211Metalloproteinase inhibitor 3Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 91PROSITE-ProRule annotation
Disulfide bondi26 ↔ 118PROSITE-ProRule annotation
Disulfide bondi36 ↔ 143PROSITE-ProRule annotation
Disulfide bondi145 ↔ 192PROSITE-ProRule annotation
Disulfide bondi150 ↔ 155PROSITE-ProRule annotation
Disulfide bondi163 ↔ 184PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP39876
PeptideAtlasiP39876
PRIDEiP39876

PTM databases

PhosphoSitePlusiP39876

Expressioni

Tissue specificityi

Highest levels are found in kidney, lung and brain followed by ovary and uterus. Low levels are found in bone.

Inductioni

Highly induced by phorbol ester (PMA), EGF and transforming growth factor-beta 1. Also induced by dexamethasone.

Gene expression databases

BgeeiENSMUSG00000020044 Expressed in 295 organ(s), highest expression level in pigmented layer of retina
CleanExiMM_TIMP3
ExpressionAtlasiP39876 baseline and differential
GenevisibleiP39876 MM

Interactioni

Subunit structurei

Interacts with EFEMP1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204204, 1 interactor
STRINGi10090.ENSMUSP00000020234

Structurei

3D structure databases

ProteinModelPortaliP39876
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 143NTRPROSITE-ProRule annotationAdd BLAST120

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 27Involved in metalloproteinase-bindingBy similarity4
Regioni88 – 89Involved in metalloproteinase-bindingBy similarity2
Regioni105 – 188Mediates interaction with EFEMP1By similarityAdd BLAST84

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4745 Eukaryota
ENOG41103NU LUCA
GeneTreeiENSGT00390000004555
HOGENOMiHOG000285981
HOVERGENiHBG068749
InParanoidiP39876
KOiK16866
OMAiCHYLPCF
OrthoDBiEOG091G0NIC
PhylomeDBiP39876
TreeFamiTF317409

Family and domain databases

Gene3Di3.90.370.10, 1 hit
InterProiView protein in InterPro
IPR001134 Netrin_domain
IPR001820 TIMP
IPR008993 TIMP-like_OB-fold
IPR015612 TIMP3
IPR027465 TIMP_C
IPR030490 TIMP_CS
PANTHERiPTHR11844 PTHR11844, 1 hit
PTHR11844:SF22 PTHR11844:SF22, 1 hit
PfamiView protein in Pfam
PF00965 TIMP, 1 hit
SMARTiView protein in SMART
SM00206 NTR, 1 hit
SUPFAMiSSF50242 SSF50242, 1 hit
PROSITEiView protein in PROSITE
PS50189 NTR, 1 hit
PS00288 TIMP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P39876-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTPWLGLVVL LSCWSLGHWG AEACTCSPSH PQDAFCNSDI VIRAKVVGKK
60 70 80 90 100
LVKEGPFGTL VYTIKQMKMY RGFSKMPHVQ YIHTEASESL CGLKLEVNKY
110 120 130 140 150
QYLLTGRVYE GKMYTGLCNF VERWDHLTLS QRKGLNYRYH LGCNCKIKSC
160 170 180 190 200
YYLPCFVTSK NECLWTDMLS NFGYPGYQSK HYACIRQKGG YCSWYRGWAP
210
PDKSISNATD P
Length:211
Mass (Da):24,182
Last modified:February 1, 1995 - v1
Checksum:i184EDF5B8D69B07B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QAB2E9QAB2_MOUSE
Metalloproteinase inhibitor 3
Timp3
50Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31P → S in AAH14713 (PubMed:15489334).Curated1
Sequence conflicti53K → T in AAH14713 (PubMed:15489334).Curated1
Sequence conflicti148K → N in AAH14713 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27424 mRNA Translation: AAA40447.1
Z30970 mRNA Translation: CAA83218.1
L19622 mRNA Translation: AAC37669.1
U26437
, U26433, U26434, U26435, U26436 Genomic DNA Translation: AAA85860.1
AK083905 mRNA Translation: BAC39055.1
BC014713 mRNA Translation: AAH14713.1
CCDSiCCDS24097.1
PIRiA53532
RefSeqiNP_035725.1, NM_011595.2
UniGeneiMm.4871

Genome annotation databases

EnsembliENSMUST00000020234; ENSMUSP00000020234; ENSMUSG00000020044
GeneIDi21859
KEGGimmu:21859
UCSCiuc007gnr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27424 mRNA Translation: AAA40447.1
Z30970 mRNA Translation: CAA83218.1
L19622 mRNA Translation: AAC37669.1
U26437
, U26433, U26434, U26435, U26436 Genomic DNA Translation: AAA85860.1
AK083905 mRNA Translation: BAC39055.1
BC014713 mRNA Translation: AAH14713.1
CCDSiCCDS24097.1
PIRiA53532
RefSeqiNP_035725.1, NM_011595.2
UniGeneiMm.4871

3D structure databases

ProteinModelPortaliP39876
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204204, 1 interactor
STRINGi10090.ENSMUSP00000020234

Protein family/group databases

MEROPSiI35.003

PTM databases

PhosphoSitePlusiP39876

Proteomic databases

PaxDbiP39876
PeptideAtlasiP39876
PRIDEiP39876

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020234; ENSMUSP00000020234; ENSMUSG00000020044
GeneIDi21859
KEGGimmu:21859
UCSCiuc007gnr.1 mouse

Organism-specific databases

CTDi7078
MGIiMGI:98754 Timp3

Phylogenomic databases

eggNOGiKOG4745 Eukaryota
ENOG41103NU LUCA
GeneTreeiENSGT00390000004555
HOGENOMiHOG000285981
HOVERGENiHBG068749
InParanoidiP39876
KOiK16866
OMAiCHYLPCF
OrthoDBiEOG091G0NIC
PhylomeDBiP39876
TreeFamiTF317409

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Miscellaneous databases

PROiPR:P39876
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020044 Expressed in 295 organ(s), highest expression level in pigmented layer of retina
CleanExiMM_TIMP3
ExpressionAtlasiP39876 baseline and differential
GenevisibleiP39876 MM

Family and domain databases

Gene3Di3.90.370.10, 1 hit
InterProiView protein in InterPro
IPR001134 Netrin_domain
IPR001820 TIMP
IPR008993 TIMP-like_OB-fold
IPR015612 TIMP3
IPR027465 TIMP_C
IPR030490 TIMP_CS
PANTHERiPTHR11844 PTHR11844, 1 hit
PTHR11844:SF22 PTHR11844:SF22, 1 hit
PfamiView protein in Pfam
PF00965 TIMP, 1 hit
SMARTiView protein in SMART
SM00206 NTR, 1 hit
SUPFAMiSSF50242 SSF50242, 1 hit
PROSITEiView protein in PROSITE
PS50189 NTR, 1 hit
PS00288 TIMP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTIMP3_MOUSE
AccessioniPrimary (citable) accession number: P39876
Secondary accession number(s): Q91WL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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