UniProtKB - P39868 (NIA2_BRANA)
Protein
Nitrate reductase [NADH], clone PBNBR1412
Gene
NIA2
Organism
Brassica napus (Rape)
Status
Functioni
Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
Catalytic activityi
- EC:1.7.1.1
Cofactori
Protein has several cofactor binding sites:- FADBy similarityNote: Binds 1 FAD.By similarity
- hemeBy similarityNote: Binds 1 heme group. The heme group is called cytochrome b-557.By similarity
- Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 191 | MolybdenumBy similarity | 1 | |
Metal bindingi | 574 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 597 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 | |
Binding sitei | 728 | FAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 735 | FAD; via pi-pi stackingBy similarity | 1 | |
Binding sitei | 793 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 706 – 709 | FADBy similarity | 4 | |
Nucleotide bindingi | 723 – 727 | FADBy similarity | 5 | |
Nucleotide bindingi | 740 – 742 | FADBy similarity | 3 |
GO - Molecular functioni
- FAD binding Source: UniProtKB
- heme binding Source: InterPro
- molybdenum ion binding Source: UniProtKB
- molybdopterin cofactor binding Source: InterPro
- nitrate reductase (NADH) activity Source: UniProtKB-EC
- nitrate reductase (NADPH) activity Source: InterPro
GO - Biological processi
- nitrate assimilation Source: UniProtKB-KW
- nitric oxide biosynthetic process Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrate assimilation |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NIA2 |
Organismi | Brassica napus (Rape) |
Taxonomic identifieri | 3708 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166052 | 1 – 911 | Nitrate reductase [NADH], clone PBNBR1412Add BLAST | 911 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 430 | InterchainSequence analysis |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Homodimer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 539 – 614 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 654 – 766 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 113 |
Sequence similaritiesi
Belongs to the nitrate reductase family.Curated
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit 3.40.50.80, 1 hit 3.90.420.10, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR014756, Ig_E-set IPR005066, MoCF_OxRdtse_dimer IPR008335, Mopterin_OxRdtase_euk IPR012137, Nitr_rd_NADH IPR001433, OxRdtase_FAD/NAD-bd IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR022407, OxRdtase_Mopterin_BS IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00970, FAD_binding_6, 1 hit PF03404, Mo-co_dimer, 1 hit PF00175, NAD_binding_1, 1 hit PF00174, Oxidored_molyb, 1 hit |
PIRSFi | PIRSF000233, Nitr_rd_NADH, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00363, CYTOCHROMEB5 PR00407, EUMOPTERIN PR00371, FPNCR |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF55856, SSF55856, 1 hit SSF56524, SSF56524, 1 hit SSF63380, SSF63380, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS51384, FAD_FR, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
i Sequence
Sequence statusi: Complete.
P39868-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATSVDNRHY PRLNPAINGV VRSFKPPPIP SPRHQNKTVS FLTEKVIVKE
60 70 80 90 100
TKNDAVDDSY DSSDDEDESH NRNVSYYKEM IRKSHSDVEP SILDSRDEST
110 120 130 140 150
ADNWIHRNSS MVRLTGKHPF NAEPPLPRLM HHGFITPVPL HYVRNHGTVP
160 170 180 190 200
KADWSEWTVE ITGLVKRPAK FTMEELISEF PSREFPVTLV CAGNRRKEQN
210 220 230 240 250
MVKQTIGFNW GSAGVSTSLW KGVPLSEILG RCGIYSRRGG GLNVCFEGAE
260 270 280 290 300
DLPGGGGSKY GTSIKKEMAM DPARDIILAY MQNGELLTPD HGFPVRIIVP
310 320 330 340 350
GFIGGRMVKW LKRIIVTPQE SDSYYHYKDN RVLPSLVDAE LANAEAWWYK
360 370 380 390 400
PEYIINELNI NSVITTPGHQ EILPINAFTT QKPYTLKGYA YSGGGKKVTR
410 420 430 440 450
VEVTLDGGDT WSVCDLDHQE KPNKYGKFWC WCFWSLDVEV LDLLSAKDVA
460 470 480 490 500
VRAWDESFNT QPDKLIWNLM GMMNNCWFRI KTNVCKPHKG EIGIVFEHPT
510 520 530 540 550
RPGNQSGGWM AKERQLEISS ESNPILKKSV SSPFMNTSAK MYSMSEVRKH
560 570 580 590 600
NSVESAWIIV HGHIYDCTRF LKDHPGGSDS ILINAGTDCT EEFEAIHSDK
610 620 630 640 650
AKKLLEDYRI GELITTGYDS SPNVSVHGGS TAVSLLAPIK ELAPSKNIAL
660 670 680 690 700
VNPREKVPVT LIEKTSISHD VRRFRFALPS EDQQLGLPVG KHIFLCANIN
710 720 730 740 750
DKLCLRAYTP TSTVDAVGHI DLVVKVYFKD VHPRFPNGGL MSQHLDSLPI
760 770 780 790 800
GSVLNIKGPL GHIEYLGKGN FMVTGKPKFA KKLAMLAGGP GITPIYQVIQ
810 820 830 840 850
SILSDPEDET EMFVVYANRT EDDILVREEL EGWANKHKDR LKVWYVVEIA
860 870 880 890 900
KEGWNYSTGF ITEPVLREHV PEGLEGESLA LACGPPPMIQ FALQPNLEKM
910
GYNVKEDLLI F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D38220 mRNA Translation: BAA07395.1 |
PIRi | T08108 |
RefSeqi | NP_001303048.1, NM_001316119.1 |
Genome annotation databases
GeneIDi | 106368948 |
KEGGi | bna:106368948 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D38220 mRNA Translation: BAA07395.1 |
PIRi | T08108 |
RefSeqi | NP_001303048.1, NM_001316119.1 |
3D structure databases
SMRi | P39868 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 106368948 |
KEGGi | bna:106368948 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit 3.40.50.80, 1 hit 3.90.420.10, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR014756, Ig_E-set IPR005066, MoCF_OxRdtse_dimer IPR008335, Mopterin_OxRdtase_euk IPR012137, Nitr_rd_NADH IPR001433, OxRdtase_FAD/NAD-bd IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR022407, OxRdtase_Mopterin_BS IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00970, FAD_binding_6, 1 hit PF03404, Mo-co_dimer, 1 hit PF00175, NAD_binding_1, 1 hit PF00174, Oxidored_molyb, 1 hit |
PIRSFi | PIRSF000233, Nitr_rd_NADH, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00363, CYTOCHROMEB5 PR00407, EUMOPTERIN PR00371, FPNCR |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF55856, SSF55856, 1 hit SSF56524, SSF56524, 1 hit SSF63380, SSF63380, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS51384, FAD_FR, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIA2_BRANA | |
Accessioni | P39868Primary (citable) accession number: P39868 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |