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UniProtKB - P39847 (PPSC_BACSU)
Protein
Plipastatin synthase subunit C
Gene
ppsC
Organism
Bacillus subtilis (strain 168)
Status
Functioni
This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Glu and Ala/Val as part of the biosynthesis of the lipopeptide antibiotic plipastatin. The Ala/Val residue is further epimerized to the D-isomer form. The activation sites for these amino acids consist of individual domains.
1 PublicationCofactori
pantetheine 4'-phosphateCuratedNote: Binds 2 phosphopantetheines covalently.Curated
GO - Molecular functioni
- acyl carrier activity Source: GO_Central
- ligase activity Source: UniProtKB-KW
- phosphopantetheine binding Source: GO_Central
- transferase activity Source: UniProtKB-KW
GO - Biological processi
- antibiotic biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase, Multifunctional enzyme, Transferase |
Biological process | Antibiotic biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU18320-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Plipastatin synthase subunit C (EC:2.3.1.-)Alternative name(s): Peptide synthase 3 Including the following 2 domains: ATP-dependent glutamate adenylase 2 Short name: GluA 2 Alternative name(s): Glutamate activase 2 ATP-dependent alanine/valine adenylase Short name: Ala/ValA Alternative name(s): Alanine/valine activase |
Gene namesi | Name:ppsC Synonyms:pps3 Ordered Locus Names:BSU18320 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193187 | 1 – 2555 | Plipastatin synthase subunit CAdd BLAST | 2555 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1002 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 | |
Modified residuei | 2038 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Phosphopantetheine, PhosphoproteinProteomic databases
PaxDbi | P39847 |
PRIDEi | P39847 |
Interactioni
Protein-protein interaction databases
IntActi | P39847, 16 interactors |
STRINGi | 224308.BSU18320 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 967 – 1042 | Carrier 1PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 2003 – 2077 | Carrier 2PROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 7 – 306 | Condensation 1Add BLAST | 300 | |
Regioni | 491 – 894 | Adenylation 1Add BLAST | 404 | |
Regioni | 1054 – 1344 | Condensation 2Add BLAST | 291 | |
Regioni | 1532 – 1927 | Adenylation 2Add BLAST | 396 | |
Regioni | 2085 – 2548 | Epimerization 3Add BLAST | 464 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1020, Bacteria |
InParanoidi | P39847 |
OMAi | AEACNFQ |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.30.300.30, 2 hits 3.30.559.10, 3 hits 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR010060, NRPS_synth IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00501, AMP-binding, 2 hits PF13193, AMP-binding_C, 2 hits PF00668, Condensation, 3 hits PF00550, PP-binding, 2 hits |
SMARTi | View protein in SMART SM00823, PKS_PP, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 2 hits TIGR01720, NRPS-para261, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 2 hits PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
P39847-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPQQPEIQDI YPLSFMQEGM LFHSLYDEQS RAYFEQASFT IHGQLDLERF
60 70 80 90 100
QKSMDAVFDR YDIFRTAFIY KNVAKPRQVV LKQRHCPIHI EDISHLNERD
110 120 130 140 150
KEHCTEAFKE QDKSKGFDLQ TDVLMRISIL KWAPDHYVCI WSHHHILMDG
160 170 180 190 200
WCLGIVIKDF LHIYQALGKG QLPDLPPVQP YGTYIKWLMQ QDREEAAEYW
210 220 230 240 250
KKRLQHFEKS TPLPKRTDQI PNGTLQQITF AIPEKETAEL QKIAAASGAT
260 270 280 290 300
LNTVFQALWG IMLQKVNRSS DAVFGSVISG RPSELKDVEN MVGLFINTIP
310 320 330 340 350
IRAQSDSLSF SDLVRRMQKD MNEAEAYSYF PLYDIQAQSA LKQELIDHII
360 370 380 390 400
VFENTPTQQE IEELNQAGSF DFSVKDFEME EVTNYSCSVK VIPGRTLYVR
410 420 430 440 450
IHFQTSAYQP SMMSEIKDYL LHMVSDVISD PSLPVSKMTL LDEDKTRKIV
460 470 480 490 500
SQNNRTVSVS PEAPTLHGLF ERQAAVTPER LAIRFSGGSL TYAELDMYAS
510 520 530 540 550
RLAAHLAARG VTNESIVGVL SERSPDMLIA VLAVLKAGGA YLPLDPAYPK
560 570 580 590 600
ERLSYMLKDS GASLLLTQPG CSAPNFSGET LEVDMTSLEC EEVKRHVSAS
610 620 630 640 650
VSDGSLAYVI YTSGSTGQPK GVAVEHRQAV SFLTGMQHQF RLSEDDIVMV
660 670 680 690 700
KTSFSFDASV WQLFWWALSG ASAYLLPPGW EKDSALIVQA IHQENVTTAH
710 720 730 740 750
FIPAMLNSFL DQAEIERLSD RTSLKRVFAG GEPLAPRTAA RFASVLPQVS
760 770 780 790 800
LIHGYGPTEA TVDAAFYVLD PERDRDRLRI PIGKPVPGAR LYVLDPHLAV
810 820 830 840 850
QPSGVAGELY IAGAGVARGY LNRPALTEER FLEDPFYLGE RMYKTGDVAR
860 870 880 890 900
WLPDGNVEFL GRTDDQVKIR GYRIEPGEIE AALRSIEGVR EAAVTVRTDS
910 920 930 940 950
GEPELCAYVE GLQRNEVRAQ LQRLLPGYMV PAYMIEMEQW PVTPSGKLDR
960 970 980 990 1000
NALPAPGGAA DAETYTAPRN VTEMKLSQLW EDVLKNGPVG IHDNFFDRGG
1010 1020 1030 1040 1050
HSLKATALVS RIAKEFDVQV PLKDVFAHPT VEGLATVIRE GTDSPYEAIK
1060 1070 1080 1090 1100
PAEKQETYPV SSAQKRIYVL QQLEDGGTGY NMPAVLELEG KLNLERMDRA
1110 1120 1130 1140 1150
FKELIKRHES LRTAFEQDAG GDPVQRIHDE VPFTLQTTVL GARTEEEAAA
1160 1170 1180 1190 1200
AFIKPFDLSQ APLFRAQIVK VSDERHLLLV DMHHIISDGV SVNILIREFG
1210 1220 1230 1240 1250
ELYNNRKLPA LRIQYKDYAV WQEGFKTGDA YKTQGAYWLK QLEGELPVLD
1260 1270 1280 1290 1300
LPADHARPPM RSFAGDKVSF TLDQEVTSGL YKLARENGST LYMVLLAAYT
1310 1320 1330 1340 1350
AFLSRLSGQE DIIVGSPIAG RPHKDLEPIL GMFVNTLALR TRPEGGKPFV
1360 1370 1380 1390 1400
QYLQEVRETA MEAFEHQDYP FEELVDKLEL TRDMSRNPLF DVMFVLQNMD
1410 1420 1430 1440 1450
QESLELDELC LKPAANNGHQ TSKFDLTLYA QEQPRGLLTF QMEFSTDLYK
1460 1470 1480 1490 1500
KKTIEKWLQY FNNMLLSIIK DNKAALGTIN ILNEDEAHYL IHELNRTKID
1510 1520 1530 1540 1550
YPRNETISRL FEMQAEQTPN AVAIVSDTQV FTYEDLNSWA NQIASVLQIK
1560 1570 1580 1590 1600
GVGPDSVVAL LTGRTPELIA GMLGILKAGG AYLPIDSNLP VERIAYMLSD
1610 1620 1630 1640 1650
SRAALLLQSE KTEKRLLGIE CEQIIIEDIQ KQGEAKNVES SAGPHSLAYI
1660 1670 1680 1690 1700
IYTSGSTGKP KGVMIEQRSV IRLVKNSNYI TFTPEDRLLM TSSIGFDVGS
1710 1720 1730 1740 1750
FEIFGPLLNG AALHLSDQQT FLDSHQLKRY IEHQGITTIW LTSSLFNHLT
1760 1770 1780 1790 1800
EQNEQTFSQL KHLIIGGEAL SPSHVNRIRN VCPEVSIWNG YGPTENTTFS
1810 1820 1830 1840 1850
TCLHIQKTYE LSIPIGRPVG NSTAFILNQW GVLQPVGAVG ELCVGGDGVA
1860 1870 1880 1890 1900
RGYLGRPDLT KEKFVPHPFA PGDRLYRTGD LARWLSDGTI EYVGRIDDQV
1910 1920 1930 1940 1950
KVRGYRVELG EIETALRQID GVKEAAVLAR TAQTGSKELF GYISVKAGTN
1960 1970 1980 1990 2000
AEQVRSLLAR SLPNYMIPAY IIEMETLPLT SNGKLNRKAL PEPDVASKQT
2010 2020 2030 2040 2050
YIPPRNELEE QLALIWQEVL GIQRIGIEDS FFELGGDSIK ALQVSARLGR
2060 2070 2080 2090 2100
YGLSLQVSDL FRHPKIKDLS PFIRKSERII EQGPIQGDVP WTPVQQWFFS
2110 2120 2130 2140 2150
QDIEERHHFN QSVMLFHSGR LSENALRPAL KKLAEHHDAL RMVYRNDDRR
2160 2170 2180 2190 2200
WIQINQGIHE SQLYSLRISD LSQSESGWET KIKQEVADLQ QSINLQEGPL
2210 2220 2230 2240 2250
LHAALFKTLT GDYLFLAIHH LVVDGVSWRI LLEDLSAGYQ QAAAGQTIQL
2260 2270 2280 2290 2300
PPKTDSYQEY ARRIQEYAQS SKLIREEAYW RSVEEQQAAE LPYEIPHHVN
2310 2320 2330 2340 2350
IDFSKRDSLS FSLTEADTAV LLQNVNHAYG TDTQDILLTA ASLAICEWTG
2360 2370 2380 2390 2400
GSKLRIAMEG HGREHILPEL DISRTVGWFT SMYPALISFE NHRDELGTSV
2410 2420 2430 2440 2450
KTVKDTLGRI PNKGVGYGML KYLTHPENKS ITFSKTPEIS FNYLGQFNDI
2460 2470 2480 2490 2500
ERQDTFRPSS LGSGKDITHT WKREQIIEMS AMAADKKLHF NLSYPPARFH
2510 2520 2530 2540 2550
RNTMEQLINR IEHFLLDIMK HCAGQQKAEK TLSDFSSQSL TAEDLDSISS
LVEEL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13715.1 Z34883 Genomic DNA Translation: CAA84362.1 |
PIRi | C69681 |
RefSeqi | NP_389714.1, NC_000964.3 WP_009967356.1, NZ_CP053102.1 |
Genome annotation databases
EnsemblBacteriai | CAB13715; CAB13715; BSU_18320 |
GeneIDi | 940102 |
KEGGi | bsu:BSU18320 |
PATRICi | fig|224308.179.peg.1999 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL009126 Genomic DNA Translation: CAB13715.1 Z34883 Genomic DNA Translation: CAA84362.1 |
PIRi | C69681 |
RefSeqi | NP_389714.1, NC_000964.3 WP_009967356.1, NZ_CP053102.1 |
3D structure databases
AlphaFoldDBi | P39847 |
SMRi | P39847 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P39847, 16 interactors |
STRINGi | 224308.BSU18320 |
Proteomic databases
PaxDbi | P39847 |
PRIDEi | P39847 |
Genome annotation databases
EnsemblBacteriai | CAB13715; CAB13715; BSU_18320 |
GeneIDi | 940102 |
KEGGi | bsu:BSU18320 |
PATRICi | fig|224308.179.peg.1999 |
Phylogenomic databases
eggNOGi | COG1020, Bacteria |
InParanoidi | P39847 |
OMAi | AEACNFQ |
Enzyme and pathway databases
BioCyci | BSUB:BSU18320-MONOMER |
Family and domain databases
Gene3Di | 1.10.1200.10, 1 hit 3.30.300.30, 2 hits 3.30.559.10, 3 hits 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR010060, NRPS_synth IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site |
Pfami | View protein in Pfam PF00501, AMP-binding, 2 hits PF13193, AMP-binding_C, 2 hits PF00668, Condensation, 3 hits PF00550, PP-binding, 2 hits |
SMARTi | View protein in SMART SM00823, PKS_PP, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 2 hits TIGR01720, NRPS-para261, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 2 hits PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPSC_BACSU | |
Accessioni | P39847Primary (citable) accession number: P39847 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1999 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families