UniProtKB - P39838 (RCSD_ECOLI)
Protein
Phosphotransferase RcsD
Gene
rcsD
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of the Rcs signaling system, which controls transcription of numerous genes. RcsD is a phosphotransfer intermediate between the sensor kinase RcsC and the response regulator RcsB. It acquires a phosphoryl group from RcsC and transfers it to RcsB. The system controls expression of genes involved in colanic acid capsule synthesis, biofilm formation and cell division.UniRule annotation5 Publications
Caution
Contains a histidine kinase domain, but it seems to be non-functional as the highly conserved histidine residue is missing.1 Publication
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- histidine phosphotransfer kinase activity Source: EcoCyc
- phosphorelay sensor kinase activity Source: GO_Central
- phosphotransferase activity, carboxyl group as acceptor Source: UniProtKB-UniRule
GO - Biological processi
- phosphorelay signal transduction system Source: EcoCyc
- protein autophosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12385-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphotransferase RcsDUniRule annotation (EC:2.7.2.-UniRule annotation)Alternative name(s): Phosphotransfer intermediate RcsDUniRule annotation |
Gene namesi | Name:rcsDUniRule annotation Synonyms:yojN, yojP, yojQ Ordered Locus Names:b2216, JW2204 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 21 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 22 – 42 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 43 – 308 | PeriplasmicSequence analysisAdd BLAST | 266 | |
Transmembranei | 309 – 329 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 330 – 890 | CytoplasmicSequence analysisAdd BLAST | 561 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 842 | H → R: Lack of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074916 | 1 – 890 | Phosphotransferase RcsDAdd BLAST | 890 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 842 | PhosphohistidineUniRule annotation1 Publication | 1 |
Post-translational modificationi
Phosphorylated by RcsC.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P39838 |
PaxDbi | P39838 |
PRIDEi | P39838 |
PTM databases
iPTMneti | P39838 |
Interactioni
Subunit structurei
Interacts with RcsC and RcsB. Has a higher affinity for RcsB than for RcsC.
UniRule annotation1 PublicationBinary interactionsi
P39838
With | #Exp. | IntAct |
---|---|---|
rcsB [P0DMC7] | 5 | EBI-556803,EBI-369670 |
Protein-protein interaction databases
BioGRIDi | 4261921, 3 interactors 851058, 1 interactor |
DIPi | DIP-12815N |
IntActi | P39838, 4 interactors |
STRINGi | 511145.b2216 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P39838 |
SMRi | P39838 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P39838 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 803 – 890 | HPtUniRule annotationAdd BLAST | 88 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 468 – 678 | Histidine-like kinaseAdd BLAST | 211 |
Sequence similaritiesi
Belongs to the RcsD family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0642, Bacteria COG2198, Bacteria |
HOGENOMi | CLU_009611_0_0_6 |
Family and domain databases
CDDi | cd00088, HPT, 1 hit |
Gene3Di | 1.20.120.160, 1 hit 3.30.565.10, 1 hit 3.40.50.11620, 1 hit |
HAMAPi | MF_00980, RcsD, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR036641, HPT_dom_sf IPR030861, Ptransferase_RcsD IPR032306, RcsD_ABL IPR038616, RcsD_ABL_sf IPR008207, Sig_transdc_His_kin_Hpt_dom |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF01627, Hpt, 1 hit PF16359, RcsD_ABL, 1 hit |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF47226, SSF47226, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50894, HPT, 1 hit |
i Sequence
Sequence statusi: Complete.
P39838-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRQKETTATT RFSLLPGSIT RFFLLLIIVL LVTMGVMVQS AVNAWLKDKS
60 70 80 90 100
YQIVDITHAI QKRVDNWRYV TWQIYDNIAA TTSPSSGEGL QETRLKQDVY
110 120 130 140 150
YLEKPRRKTE ALIFGSHDNS TLEMTQRMST YLDTLWGAEN VPWSMYYLNG
160 170 180 190 200
QDNSLVLIST LPLKDLTSGF KESTVSDIVD SRRAEMLQQA NALDERESFS
210 220 230 240 250
NMRRLAWQNG HYFTLRTTFN QPGHLATVVA FDLPINDLIP PGMPLDSFRL
260 270 280 290 300
EPDATATGNN DNEKEGTDSV SIHFNSTKIE ISSALNSTDM RLVWQVPYGT
310 320 330 340 350
LLLDTLQNIL LPLLLNIGLL ALALFGYTTF RHFSSRSTEN VPSTAVNNEL
360 370 380 390 400
RILRAINEEI VSLLPLGLLV HDQESNRTVI SNKIADHLLP HLNLQNITTM
410 420 430 440 450
AEQHQGIIQA TINNELYEIR MFRSQVAPRT QIFIIRDQDR EVLVNKKLKQ
460 470 480 490 500
AQRLYEKNQQ GRMIFMKNIG DALKEPAQSL AESAAKLNAP ESKQLANQAD
510 520 530 540 550
VLVRLVDEIQ LANMLADDSW KSETVLFSVQ DLIDEVVPSV LPAIKRKGLQ
560 570 580 590 600
LLINNHLKAH DMRRGDRDAL RRILLLLMQY AVTSTQLGKI TLEVDQDESS
610 620 630 640 650
EDRLTFRILD TGEGVSIHEM DNLHFPFINQ TQNDRYGKAD PLAFWLSDQL
660 670 680 690 700
ARKLGGHLNI KTRDGLGTRY SVHIKMLAAD PEVEEEEERL LDDVCVMVDV
710 720 730 740 750
TSAEIRNIVT RQLENWGATC ITPDERLISQ DYDIFLTDNP SNLTASGLLL
760 770 780 790 800
SDDESGVREI GPGQLCVNFN MSNAMQEAVL QLIEVQLAQE EVTESPLGGD
810 820 830 840 850
ENAQLHASGY YALFVDTVPD DVKRLYTEAA TSDFAALAQT AHRLKGVFAM
860 870 880 890
LNLVPGKQLC ETLEHLIREK DVPGIEKYIS DIDSYVKSLL
Sequence cautioni
The sequence AAA81025 differs from that shown. Reason: Frameshift.Curated
The sequence AAA81026 differs from that shown. Reason: Frameshift.Curated
The sequence AAA81027 differs from that shown. Reason: Frameshift.Curated
The sequence M28242 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | Q → L in AAA81025 (Ref. 4) Curated | 1 | |
Sequence conflicti | 164 | K → E in AAA81026 (Ref. 4) Curated | 1 | |
Sequence conflicti | 174 | T → A in AAA81026 (Ref. 4) Curated | 1 | |
Sequence conflicti | 176 | S → N in AAA81026 (Ref. 4) Curated | 1 | |
Sequence conflicti | 179 | V → G in AAA81026 (Ref. 4) Curated | 1 | |
Sequence conflicti | 184 | A → T in AAA81026 (Ref. 4) Curated | 1 | |
Sequence conflicti | 267 | T → A in AAA81026 (Ref. 4) Curated | 1 | |
Sequence conflicti | 598 – 599 | ES → DA in M28242 (PubMed:2404948).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75276.1 AP009048 Genomic DNA Translation: BAA15999.1 U38659 Genomic DNA Translation: AAA81025.1 Frameshift. U38659 Genomic DNA Translation: AAA81026.1 Frameshift. U38659 Genomic DNA Translation: AAA81027.1 Frameshift. M28242 Genomic DNA No translation available. |
PIRi | F64991 |
RefSeqi | NP_416720.1, NC_000913.3 WP_001249081.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75276; AAC75276; b2216 BAA15999; BAA15999; BAA15999 |
GeneIDi | 946717 |
KEGGi | ecj:JW2204 eco:b2216 |
PATRICi | fig|1411691.4.peg.19 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75276.1 AP009048 Genomic DNA Translation: BAA15999.1 U38659 Genomic DNA Translation: AAA81025.1 Frameshift. U38659 Genomic DNA Translation: AAA81026.1 Frameshift. U38659 Genomic DNA Translation: AAA81027.1 Frameshift. M28242 Genomic DNA No translation available. |
PIRi | F64991 |
RefSeqi | NP_416720.1, NC_000913.3 WP_001249081.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SR2 | NMR | - | A | 775-890 | [»] | |
2KX7 | NMR | - | A | 688-795 | [»] | |
BMRBi | P39838 | |||||
SMRi | P39838 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261921, 3 interactors 851058, 1 interactor |
DIPi | DIP-12815N |
IntActi | P39838, 4 interactors |
STRINGi | 511145.b2216 |
PTM databases
iPTMneti | P39838 |
Proteomic databases
jPOSTi | P39838 |
PaxDbi | P39838 |
PRIDEi | P39838 |
Genome annotation databases
EnsemblBacteriai | AAC75276; AAC75276; b2216 BAA15999; BAA15999; BAA15999 |
GeneIDi | 946717 |
KEGGi | ecj:JW2204 eco:b2216 |
PATRICi | fig|1411691.4.peg.19 |
Organism-specific databases
EchoBASEi | EB2286 |
Phylogenomic databases
eggNOGi | COG0642, Bacteria COG2198, Bacteria |
HOGENOMi | CLU_009611_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12385-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P39838 |
PROi | PR:P39838 |
Family and domain databases
CDDi | cd00088, HPT, 1 hit |
Gene3Di | 1.20.120.160, 1 hit 3.30.565.10, 1 hit 3.40.50.11620, 1 hit |
HAMAPi | MF_00980, RcsD, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR036641, HPT_dom_sf IPR030861, Ptransferase_RcsD IPR032306, RcsD_ABL IPR038616, RcsD_ABL_sf IPR008207, Sig_transdc_His_kin_Hpt_dom |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF01627, Hpt, 1 hit PF16359, RcsD_ABL, 1 hit |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF47226, SSF47226, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50894, HPT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RCSD_ECOLI | |
Accessioni | P39838Primary (citable) accession number: P39838 Secondary accession number(s): P47725, P47726, P76456 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families