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UniProtKB - P39816 (PTW3C_BACSU)
Protein
Putative PTS system glucosamine-specific EIICBA component
Gene
gamP
Organism
Bacillus subtilis (strain 168)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system may be involved in glucosamine transport.
By similarity1 PublicationCatalytic activityi
- Nπ-phospho-L-histidyl-[protein] + N-acetyl-D-glucosamine(out) = L-histidyl-[protein] + N-acetyl-D-glucosamine 6-phosphate(in)By similarityEC:2.7.1.193By similarity
Cofactori
Zn2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 419 | Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 552 | ZincBy similarity | 1 | |
Sitei | 552 | Important for phospho-donor activityBy similarity | 1 | |
Active sitei | 567 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 567 | ZincBy similarity | 1 |
GO - Molecular functioni
- glucose transmembrane transporter activity Source: InterPro
- kinase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro
- protein-phosphocysteine-sugar phosphotransferase activity Source: GO_Central
GO - Biological processi
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU02350-MONOMER |
Protein family/group databases
TCDBi | 4.A.1.1.6, the pts glucose-glucoside (glc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Putative PTS system glucosamine-specific EIICBA component1 PublicationIncluding the following 3 domains: Glucosamine permease IIC component1 Publication Alternative name(s): PTS system glucosamine-specific EIIC component1 Publication Glucosamine-specific phosphotransferase enzyme IIB componentBy similarity (EC:2.7.1.193By similarity) Alternative name(s): PTS system glucosamine-specific EIIB componentBy similarity Glucosamine-specific phosphotransferase enzyme IIA componentBy similarity Alternative name(s): PTS system glucosamine-specific EIIA componentBy similarity |
Gene namesi | Name:gamP1 Publication Synonyms:ybfS, yzfA Ordered Locus Names:BSU02350 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 56 – 76 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 196 – 216 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 243 – 263 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 274 – 294 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 298 – 318 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 350 – 370 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186710 | 1 – 631 | Putative PTS system glucosamine-specific EIICBA componentAdd BLAST | 631 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 419 | Phosphocysteine; by EIIABy similarityCurated | 1 | |
Modified residuei | 567 | Phosphohistidine; by HPrBy similarityCurated | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P39816 |
Expressioni
Inductioni
Expression is repressed by the HTH-type transcriptional regulator GamR.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 382 | PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST | 382 | |
Domaini | 397 – 478 | PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 515 – 619 | PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST | 105 |
Domaini
The PTS EIIC type-1 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-1 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-1 domain.PROSITE-ProRule annotation
The PTS EIIA type-1 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-1 domain.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1263, Bacteria COG1264, Bacteria COG2190, Bacteria |
InParanoidi | P39816 |
OMAi | ETDATIM |
PhylomeDBi | P39816 |
Family and domain databases
CDDi | cd00212, PTS_IIB_glc, 1 hit |
Gene3Di | 2.70.70.10, 1 hit 3.30.1360.60, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR036878, Glu_permease_IIB IPR018113, PTrfase_EIIB_Cys IPR001127, PTS_EIIA_1_perm IPR003352, PTS_EIIC IPR013013, PTS_EIIC_1 IPR001996, PTS_IIB_1 IPR011299, PTS_IIBC_glc |
Pfami | View protein in Pfam PF00358, PTS_EIIA_1, 1 hit PF00367, PTS_EIIB, 1 hit PF02378, PTS_EIIC, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit SSF55604, SSF55604, 1 hit |
TIGRFAMsi | TIGR00826, EIIB_glc, 1 hit TIGR00830, PTBA, 1 hit TIGR02002, PTS-II-BC-glcB, 1 hit |
PROSITEi | View protein in PROSITE PS51093, PTS_EIIA_TYPE_1, 1 hit PS00371, PTS_EIIA_TYPE_1_HIS, 1 hit PS51098, PTS_EIIB_TYPE_1, 1 hit PS01035, PTS_EIIB_TYPE_1_CYS, 1 hit PS51103, PTS_EIIC_TYPE_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P39816-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKKAFQILQ QLGRALMTPV AVLPAAGLLL RFGDKDLLNI PIIKDAGGVV
60 70 80 90 100
FDNLPLIFAV GVAIGLAGGE GVAGLAAVIG YLILTVTLDN MGKLLGLQPP
110 120 130 140 150
YEGAEHLIDM GVFGGIIIGL LAAYLYKRFS SIELHPVLGF FSGKRFVPII
160 170 180 190 200
TSVSSLVIGV IFSFVWPLIQ NGINAASSLI ADSTVGLFFY ATIYRLLIPF
210 220 230 240 250
GLHHIFYTPF YFMMGEYTDP STGNTVTGDL TRFFAGDPTA GRFMMGDFPY
260 270 280 290 300
MIFCLPAVAL AIIHTARPEK KKMISGVMIS AALTSMLTGI TEPVEFSFLF
310 320 330 340 350
VAPVLYLINS ILAGVIFVVC DLFHVRHGYT FSGGGIDYVL NYGLSTNGWV
360 370 380 390 400
VIPVGIVFAF IYYYLFRFAI LKWNLKTPGR ETDEDGQNEE KAPVAKDQLA
410 420 430 440 450
FHVLQALGGQ QNIANLDACI TRLRVTVHQP SQVCKDELKR LGAVGVLEVN
460 470 480 490 500
NNFQAIFGTK SDALKDDIKT IMAGGVPATA AALDTVTDKP LKPDSDETFI
510 520 530 540 550
YPIKGETVSL GDVPDQVFSE KMMGEGFAII PSEGKVVAPA DGEIVSIFPT
560 570 580 590 600
KHAIGFMSAG GTEILIHVGI DTVKLNGEGF EAHVTSGQAV KQGELLLTFD
610 620 630
LNYIKQHAAS AITPVIFTNT SEEDLKHIQM K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006424 Genomic DNA Translation: BAA33132.1 AL009126 Genomic DNA Translation: CAB12029.1 U15147 Genomic DNA Translation: AAA82877.1 |
PIRi | D69750 |
RefSeqi | NP_388117.1, NC_000964.3 WP_003246270.1, NZ_JNCM01000030.1 |
Genome annotation databases
EnsemblBacteriai | CAB12029; CAB12029; BSU_02350 |
GeneIDi | 938418 |
KEGGi | bsu:BSU02350 |
PATRICi | fig|224308.179.peg.241 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB006424 Genomic DNA Translation: BAA33132.1 AL009126 Genomic DNA Translation: CAB12029.1 U15147 Genomic DNA Translation: AAA82877.1 |
PIRi | D69750 |
RefSeqi | NP_388117.1, NC_000964.3 WP_003246270.1, NZ_JNCM01000030.1 |
3D structure databases
AlphaFoldDBi | P39816 |
SMRi | P39816 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU02350 |
Protein family/group databases
TCDBi | 4.A.1.1.6, the pts glucose-glucoside (glc) family |
Proteomic databases
PaxDbi | P39816 |
Genome annotation databases
EnsemblBacteriai | CAB12029; CAB12029; BSU_02350 |
GeneIDi | 938418 |
KEGGi | bsu:BSU02350 |
PATRICi | fig|224308.179.peg.241 |
Phylogenomic databases
eggNOGi | COG1263, Bacteria COG1264, Bacteria COG2190, Bacteria |
InParanoidi | P39816 |
OMAi | ETDATIM |
PhylomeDBi | P39816 |
Enzyme and pathway databases
BioCyci | BSUB:BSU02350-MONOMER |
Family and domain databases
CDDi | cd00212, PTS_IIB_glc, 1 hit |
Gene3Di | 2.70.70.10, 1 hit 3.30.1360.60, 1 hit |
InterProi | View protein in InterPro IPR011055, Dup_hybrid_motif IPR036878, Glu_permease_IIB IPR018113, PTrfase_EIIB_Cys IPR001127, PTS_EIIA_1_perm IPR003352, PTS_EIIC IPR013013, PTS_EIIC_1 IPR001996, PTS_IIB_1 IPR011299, PTS_IIBC_glc |
Pfami | View protein in Pfam PF00358, PTS_EIIA_1, 1 hit PF00367, PTS_EIIB, 1 hit PF02378, PTS_EIIC, 1 hit |
SUPFAMi | SSF51261, SSF51261, 1 hit SSF55604, SSF55604, 1 hit |
TIGRFAMsi | TIGR00826, EIIB_glc, 1 hit TIGR00830, PTBA, 1 hit TIGR02002, PTS-II-BC-glcB, 1 hit |
PROSITEi | View protein in PROSITE PS51093, PTS_EIIA_TYPE_1, 1 hit PS00371, PTS_EIIA_TYPE_1_HIS, 1 hit PS51098, PTS_EIIB_TYPE_1, 1 hit PS01035, PTS_EIIB_TYPE_1_CYS, 1 hit PS51103, PTS_EIIC_TYPE_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PTW3C_BACSU | |
Accessioni | P39816Primary (citable) accession number: P39816 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |