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UniProtKB - P39795 (TREC_BACSU)
Protein
Trehalose-6-phosphate hydrolase
Gene
treA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Hydrolyzes trehalose-6-phosphate to glucose and glucose 6-phosphate. Can also very effectively hydrolyzes p-nitrophenyl-alpha-D-glucopyranoside, but not lactose, maltose, sucrose or sucrose-6-phosphate. Trehalose is also hydrolyzed, but to a much smaller extent than trehalose-6-phosphate.
2 PublicationsCatalytic activityi
- EC:3.2.1.932 Publications
Activity regulationi
Activity is stimulated by high salt concentrations with different efficiencies depending on the kind of salt. In vitro, inhibited by glucose.1 Publication
pH dependencei
Optimum pH is 4.5.1 Publication
Temperature dependencei
Optimum temperature is 37 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 203 | NucleophileBy similarity | 1 | |
Active sitei | 254 | Proton donorBy similarity | 1 | |
Sitei | 329 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- alpha,alpha-phosphotrehalase activity Source: UniProtKB-EC
- alpha-amylase activity Source: GO_Central
GO - Biological processi
- oligosaccharide catabolic process Source: GO_Central
- trehalose catabolic process Source: InterPro
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Enzyme and pathway databases
BioCyci | BSUB:BSU07810-MONOMER |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Trehalose-6-phosphate hydrolaseCurated (EC:3.2.1.932 Publications)Alternative name(s): Alpha,alpha-phosphotrehalase Phospho-alpha-(1-1)-glucosidase1 Publication |
Gene namesi | Name:treA1 Publication Synonyms:treC Ordered Locus Names:BSU07810 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054320 | 1 – 561 | Trehalose-6-phosphate hydrolaseAdd BLAST | 561 |
Proteomic databases
jPOSTi | P39795 |
PaxDbi | P39795 |
PRIDEi | P39795 |
Expressioni
Inductioni
Induced by trehalose and repressed by glucose, fructose or mannitol.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
InParanoidi | P39795 |
OMAi | PNGEKWA |
PhylomeDBi | P39795 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit 3.90.400.10, 1 hit |
InterProi | View protein in InterPro IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR032091, Malt_amylase_C IPR045857, O16G_dom_2 IPR012769, Trehalose_TreC |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF16657, Malt_amylase_C, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
TIGRFAMsi | TIGR02403, trehalose_treC, 1 hit |
i Sequence
Sequence statusi: Complete.
P39795-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTEQTPWWK KAVVYQIYPK SFNDTTGNGV GDLNGIIEKL DYLKTLQVDV
60 70 80 90 100
LWLTPIYDSP QHDNGYDIRD YYSIYPEYGT MEDFERLVSE AHKRDLKVVM
110 120 130 140 150
DLVVNHTSTE HKWFREAISS IDSPYRDFYI WKKPQENGSV PTNWESKFGG
160 170 180 190 200
SAWELDEASG QYYLHLFDVT QADLNWENEE VRKHVYDMMH FWFEKGIDGF
210 220 230 240 250
RLDVINLISK DQRFPNAEEG DGRSFYTDGP RVHEFLHEMN EKVFSHYDSM
260 270 280 290 300
TVGEMSSTTV DHCIRYTNPD NKELDMTFSF HHLKVDYPNG EKWALAPFDF
310 320 330 340 350
LKLKEILSDW QTGMHAGGGW NALFWCNHDQ PRVVSRYGDD GAYRVKSAKM
360 370 380 390 400
LATAIHMMQG TPYIYQGEEL GMTNPKFTDI SSYRDVESLN MYHAFKEKGM
410 420 430 440 450
ADQDITAILQ AKSRDNSRTP VQWDATENGG FTTGTPWIPV AGNYREINAE
460 470 480 490 500
AALRDQNSVF YHYQKLIQIR KMYDIVTEGT YEIIAKDDPN IFAYLRHGSN
510 520 530 540 550
EKLLVINNFY GTEAAFTLPD SLAPDEWKAE VLLTNDEARE GLQNMTLRPY
560
ESIVYRLTKP C
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 114 – 123 | FREAISSIDS → LRGDLFNRQ in CAA91015 (PubMed:7651129).Curated | 10 | |
Sequence conflicti | 196 | G → E in CAA91015 (PubMed:7651129).Curated | 1 | |
Sequence conflicti | 196 | G → E in CAA56495 (PubMed:7651129).Curated | 1 | |
Sequence conflicti | 288 – 290 | PNG → LTA in CAA91015 (PubMed:7651129).Curated | 3 | |
Sequence conflicti | 288 – 290 | PNG → LTA in CAA56495 (PubMed:7651129).Curated | 3 | |
Sequence conflicti | 386 | V → A in CAA91015 (PubMed:7651129).Curated | 1 | |
Sequence conflicti | 386 | V → A in CAA56495 (PubMed:7651129).Curated | 1 | |
Sequence conflicti | 547 – 561 | LRPYE…LTKPC → VAHMSPLFIV (PubMed:7651129).CuratedAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54245 Genomic DNA Translation: CAA91015.1 X80203 Genomic DNA Translation: CAA56495.1 D83967 Genomic DNA Translation: BAA23408.1 AL009126 Genomic DNA Translation: CAB12610.1 |
PIRi | B69725 |
RefSeqi | NP_388662.1, NC_000964.3 WP_003244272.1, NZ_JNCM01000032.1 |
Genome annotation databases
EnsemblBacteriai | CAB12610; CAB12610; BSU_07810 |
GeneIDi | 939690 |
KEGGi | bsu:BSU07810 |
PATRICi | fig|224308.179.peg.845 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z54245 Genomic DNA Translation: CAA91015.1 X80203 Genomic DNA Translation: CAA56495.1 D83967 Genomic DNA Translation: BAA23408.1 AL009126 Genomic DNA Translation: CAB12610.1 |
PIRi | B69725 |
RefSeqi | NP_388662.1, NC_000964.3 WP_003244272.1, NZ_JNCM01000032.1 |
3D structure databases
AlphaFoldDBi | P39795 |
SMRi | P39795 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU07810 |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Proteomic databases
jPOSTi | P39795 |
PaxDbi | P39795 |
PRIDEi | P39795 |
Genome annotation databases
EnsemblBacteriai | CAB12610; CAB12610; BSU_07810 |
GeneIDi | 939690 |
KEGGi | bsu:BSU07810 |
PATRICi | fig|224308.179.peg.845 |
Phylogenomic databases
eggNOGi | COG0366, Bacteria |
InParanoidi | P39795 |
OMAi | PNGEKWA |
PhylomeDBi | P39795 |
Enzyme and pathway databases
BioCyci | BSUB:BSU07810-MONOMER |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit 3.90.400.10, 1 hit |
InterProi | View protein in InterPro IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF IPR032091, Malt_amylase_C IPR045857, O16G_dom_2 IPR012769, Trehalose_TreC |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF16657, Malt_amylase_C, 1 hit |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
TIGRFAMsi | TIGR02403, trehalose_treC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TREC_BACSU | |
Accessioni | P39795Primary (citable) accession number: P39795 Secondary accession number(s): O34517 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | May 30, 2000 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families