We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P39773 (GPMI_BACSU)
Protein
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Gene
gpmI
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Essential for rapid growth and for sporulation. Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.
2 PublicationsCatalytic activityi
- EC:5.4.2.12UniRule annotation
Cofactori
Mn2+2 PublicationsNote: Binds 2 manganese ions per subunit.2 Publications
Activity regulationi
Inhibited during sporulation by acidification of the forespore, thus allowing accumulation of the spore's large depot of 3-phosphoglyceric acid.1 Publication
pH dependencei
Very sensitive to pH. A pH change from 8 to 6 results in greater than 30- to 200-fold decreases in its activity. However, deactivation at pH 6 is reversed by shifting the enzyme to pH 7 or 8.1 Publication
: glycolysis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 12 | Manganese 2UniRule annotation | 1 | |
Active sitei | 62 | Phosphoserine intermediateUniRule annotation | 1 | |
Metal bindingi | 62 | Manganese 2UniRule annotation | 1 | |
Binding sitei | 123 | SubstrateUniRule annotation | 1 | |
Binding sitei | 185 | SubstrateUniRule annotation | 1 | |
Binding sitei | 191 | SubstrateUniRule annotation | 1 | |
Binding sitei | 336 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 403 | Manganese 1UniRule annotation | 1 | |
Metal bindingi | 407 | Manganese 1UniRule annotation | 1 | |
Metal bindingi | 444 | Manganese 2UniRule annotation | 1 | |
Metal bindingi | 445 | Manganese 2UniRule annotation | 1 | |
Metal bindingi | 462 | Manganese 1UniRule annotation | 1 |
GO - Molecular functioni
- 2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity Source: UniProtKB
- manganese ion binding Source: UniProtKB
GO - Biological processi
- cellular carbohydrate metabolic process Source: GO_Central
- glucose catabolic process Source: InterPro
- glycolytic process Source: UniProtKB-UniRule
- regulation of sporulation Source: UniProtKB
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Glycolysis, Sporulation |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU33910-MONOMER |
SABIO-RKi | P39773 |
UniPathwayi | UPA00109;UER00186 |
Names & Taxonomyi
Protein namesi | Recommended name: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase1 Publication (EC:5.4.2.12UniRule annotation)Short name: BPG-independent PGAM1 Publication Short name: Phosphoglyceromutase1 Publication Short name: iPGM1 Publication Alternative name(s): Vegetative protein 107 Short name: VEG107 |
Gene namesi | Name:gpmIUniRule annotation Synonyms:pgm Ordered Locus Names:BSU33910 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000212128 | 2 – 511 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutaseAdd BLAST | 510 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 62 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P39773 |
PaxDbi | P39773 |
PRIDEi | P39773 |
PTM databases
iPTMneti | P39773 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
IntActi | P39773, 1 interactor |
MINTi | P39773 |
STRINGi | 224308.BSU33910 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 153 – 154 | Substrate bindingUniRule annotation | 2 | |
Regioni | 261 – 264 | Substrate bindingUniRule annotation | 4 |
Sequence similaritiesi
Belongs to the BPG-independent phosphoglycerate mutase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0696, Bacteria |
InParanoidi | P39773 |
OMAi | FMDGRDT |
PhylomeDBi | P39773 |
Family and domain databases
CDDi | cd16010, iPGM, 1 hit |
Gene3Di | 3.40.1450.10, 1 hit 3.40.720.10, 1 hit |
HAMAPi | MF_01038, GpmI, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR011258, BPG-indep_PGM_N IPR006124, Metalloenzyme IPR036646, PGAM_B_sf IPR005995, Pgm_bpd_ind |
PANTHERi | PTHR31637, PTHR31637, 1 hit |
Pfami | View protein in Pfam PF06415, iPGM_N, 1 hit PF01676, Metalloenzyme, 1 hit |
PIRSFi | PIRSF001492, IPGAM, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit SSF64158, SSF64158, 1 hit |
TIGRFAMsi | TIGR01307, pgm_bpd_ind, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P39773-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKKPAALII LDGFGLRNET VGNAVALAKK PNFDRYWNQY PHQTLTASGE
60 70 80 90 100
AVGLPEGQMG NSEVGHLNIG AGRIVYQSLT RVNVAIREGE FERNQTFLDA
110 120 130 140 150
ISNAKENNKA LHLFGLLSDG GVHSHINHLF ALLKLAKKEG LTKVYIHGFL
160 170 180 190 200
DGRDVGPQTA KTYINQLNDQ IKEIGVGEIA SISGRYYSMD RDKRWDRVEK
210 220 230 240 250
AYRAMAYGEG PSYRSALDVV DDSYANGIYD EFVIPSVITK ENGEPVAKIQ
260 270 280 290 300
DGDSVIFYNF RPDRAIQISN TFTNKDFRDF DRGENYPKNL YFVCLTHFSE
310 320 330 340 350
TVDGYVAFKP INLDNTVGEV LSQHGLKQLR IAETEKYPHV TFFMSGGREA
360 370 380 390 400
EFPGEERILI NSPKVATYDL KPEMSAYEVK DALVKEIEAD KHDAIILNFA
410 420 430 440 450
NPDMVGHSGM VEPTIKAIEA VDECLGEVVD AILAKGGHAI ITADHGNADI
460 470 480 490 500
LITESGEPHT AHTTNPVPVI VTKEGITLRE GGILGDLAPT LLDLLGVEKP
510
KEMTGTSLIQ K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 233 | V → C in AAA21680 (PubMed:8021172).Curated | 1 | |
Sequence conflicti | 432 – 433 | IL → MV in AAA21680 (PubMed:8021172).Curated | 2 | |
Sequence conflicti | 445 | H → D in AAA21680 (PubMed:8021172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L29475 Genomic DNA Translation: AAA21680.1 AL009126 Genomic DNA Translation: CAB15396.1 |
PIRi | D69675 |
RefSeqi | NP_391271.1, NC_000964.3 WP_003228330.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15396; CAB15396; BSU_33910 |
GeneIDi | 938574 |
KEGGi | bsu:BSU33910 |
PATRICi | fig|224308.179.peg.3676 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L29475 Genomic DNA Translation: AAA21680.1 AL009126 Genomic DNA Translation: CAB15396.1 |
PIRi | D69675 |
RefSeqi | NP_391271.1, NC_000964.3 WP_003228330.1, NZ_JNCM01000033.1 |
3D structure databases
SMRi | P39773 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P39773, 1 interactor |
MINTi | P39773 |
STRINGi | 224308.BSU33910 |
PTM databases
iPTMneti | P39773 |
Proteomic databases
jPOSTi | P39773 |
PaxDbi | P39773 |
PRIDEi | P39773 |
Genome annotation databases
EnsemblBacteriai | CAB15396; CAB15396; BSU_33910 |
GeneIDi | 938574 |
KEGGi | bsu:BSU33910 |
PATRICi | fig|224308.179.peg.3676 |
Phylogenomic databases
eggNOGi | COG0696, Bacteria |
InParanoidi | P39773 |
OMAi | FMDGRDT |
PhylomeDBi | P39773 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00186 |
BioCyci | BSUB:BSU33910-MONOMER |
SABIO-RKi | P39773 |
Family and domain databases
CDDi | cd16010, iPGM, 1 hit |
Gene3Di | 3.40.1450.10, 1 hit 3.40.720.10, 1 hit |
HAMAPi | MF_01038, GpmI, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR011258, BPG-indep_PGM_N IPR006124, Metalloenzyme IPR036646, PGAM_B_sf IPR005995, Pgm_bpd_ind |
PANTHERi | PTHR31637, PTHR31637, 1 hit |
Pfami | View protein in Pfam PF06415, iPGM_N, 1 hit PF01676, Metalloenzyme, 1 hit |
PIRSFi | PIRSF001492, IPGAM, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit SSF64158, SSF64158, 1 hit |
TIGRFAMsi | TIGR01307, pgm_bpd_ind, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GPMI_BACSU | |
Accessioni | P39773Primary (citable) accession number: P39773 Secondary accession number(s): O32250 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families