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Protein

BolA-like protein 3

Gene

BOL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates [4Fe-4S] cluster insertion into a subset of mitochondrial proteins such as LIAS and succinate dehydrogenase (SDH) (PubMed:27532773, PubMed:27532772). Required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein (PubMed:27532772). Acts together with NFU1, later than BOL1 and GRX5 in the [4Fe-4S] cluster insertion process (PubMed:27532773). Not required for [2Fe-2S] cluster insertion into mitochondrial proteins (PubMed:27532772).2 Publications

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • protein maturation by iron-sulfur cluster transfer Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-28853-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
BolA-like protein 3Curated
Alternative name(s):
Altered inheritance of mitochondria protein 1
Gene namesi
Name:BOL32 Publications
Synonyms:AIM1
Ordered Locus Names:YAL046C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL046C
SGDiS000000044 BOL3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increases frequency of mitochondrial genome loss (PubMed:19300474). Cells show a slight decrease of succinate dehydrogenase (SDH) (PubMed:27532772). Cells lacking BOL1 and BOL3 display defects in a subset of mitochondrial [4Fe-4S] enzymes (PubMed:27532773, PubMed:27532772).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64C → A: Partial loss of function. 1 Publication1
Mutagenesisi101H → A: Loss of function. 1 Publication1
Mutagenesisi101H → C: Dominant negative mutant; displays respiratory defects that are higher than the BOL1 BOL3 double mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002012391 – 118BolA-like protein 3Add BLAST118

Proteomic databases

MaxQBiP39724
PaxDbiP39724
PRIDEiP39724

Interactioni

Subunit structurei

Interacts with NFU1 (PubMed:27532773).1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31781, 111 interactors
IntActiP39724, 10 interactors
MINTiP39724
STRINGi4932.YAL046C

Structurei

3D structure databases

ProteinModelPortaliP39724
SMRiP39724
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BolA/IbaG family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013048
InParanoidiP39724
KOiK22075
OMAiKWHGLQL
OrthoDBiEOG092C5RHO

Family and domain databases

InterProiView protein in InterPro
IPR002634 BolA
IPR036065 BolA-like_sf
PANTHERiPTHR12735 PTHR12735, 1 hit
PfamiView protein in Pfam
PF01722 BolA, 1 hit
SUPFAMiSSF82657 SSF82657, 1 hit

Sequencei

Sequence statusi: Complete.

P39724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLPQTMLRS ISVKHVRWPR ILTGSKLWYS TQMAMTPEEK MITDKLQQEL
60 70 80 90 100
EPEVCKVQDV SGGCGSMFAI NITSKKFNGL SLIKQHQLVN RILRDDISRW
110
HGLQLTTKKS TGKGPASS
Length:118
Mass (Da):13,356
Last modified:February 1, 1995 - v1
Checksum:i244F5FF2052FF410
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA Translation: AAC04985.1
BK006935 Genomic DNA Translation: DAA06940.1
PIRiS51973
RefSeqiNP_009353.1, NM_001178191.1

Genome annotation databases

EnsemblFungiiYAL046C; YAL046C; YAL046C
GeneIDi851251
KEGGisce:YAL046C

Similar proteinsi

Entry informationi

Entry nameiBOL3_YEAST
AccessioniPrimary (citable) accession number: P39724
Secondary accession number(s): D6VPH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

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