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Protein

(R,R)-butanediol dehydrogenase

Gene

BDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NAD-dependent (R,R)-butanediol dehydrogenase which catalyzes oxidation of (R,R)-butane-2,3-diol to (3R)-acetoin, of meso-butanediol to (3S)-acetoin, and reduction of acetoin. Allows the use of 2,3-butanediol as an aerobic carbon source.1 Publication

Miscellaneous

Present with 8730 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=14 mM for (R,R)-butane-2,3-diol1 Publication
  2. KM=65 mM for meso-butanediol1 Publication
  3. KM=57 mM for 1,2-Butanediol1 Publication
  4. KM=4.5 mM for (3R/3S)-acetoin1 Publication
  5. KM=0.55 mM for NAD1 Publication
  6. KM=0.055 mM for NADH1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi39Zinc 1; catalyticBy similarity1
    Metal bindingi73Zinc 1; catalyticBy similarity1
    Metal bindingi103Zinc 2By similarity1
    Metal bindingi120Zinc 2By similarity1
    Metal bindingi123Zinc 2By similarity1
    Metal bindingi131Zinc 2By similarity1
    Metal bindingi173Zinc 1; catalyticBy similarity1

    GO - Molecular functioni

    • (R,R)-butanediol dehydrogenase activity Source: SGD
    • zinc ion binding Source: InterPro

    GO - Biological processi

    • alcohol metabolic process Source: SGD
    • butanediol biosynthetic process Source: SGD

    Keywordsi

    Molecular functionOxidoreductase
    LigandMetal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:YAL060W-MONOMER
    YEAST:YAL060W-MONOMER
    BRENDAi1.1.1.303 984
    1.1.1.4 984

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (R,R)-butanediol dehydrogenase (EC:1.1.1.4)
    Gene namesi
    Name:BDH1
    Synonyms:BDH
    Ordered Locus Names:YAL060W
    ORF Names:FUN49
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome I

    Organism-specific databases

    SGDiS000000056 BDH1

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001607881 – 382(R,R)-butanediol dehydrogenaseAdd BLAST382

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei63PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39714
    PaxDbiP39714
    PRIDEiP39714

    PTM databases

    CarbonylDBiP39714
    iPTMnetiP39714

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi31770, 49 interactors
    DIPiDIP-5356N
    IntActiP39714, 1 interactor
    MINTiP39714
    STRINGi4932.YAL060W

    Structurei

    3D structure databases

    ProteinModelPortaliP39714
    SMRiP39714
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00910000146556
    HOGENOMiHOG000294670
    InParanoidiP39714
    KOiK00004
    OMAiGYPDEIF
    OrthoDBiEOG092C3CGE

    Family and domain databases

    InterProiView protein in InterPro
    IPR013149 ADH_C
    IPR013154 ADH_N
    IPR002328 ADH_Zn_CS
    IPR011032 GroES-like_sf
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF08240 ADH_N, 1 hit
    PF00107 ADH_zinc_N, 1 hit
    SUPFAMiSSF50129 SSF50129, 1 hit
    SSF51735 SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00059 ADH_ZINC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P39714-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MRALAYFKKG DIHFTNDIPR PEIQTDDEVI IDVSWCGICG SDLHEYLDGP
    60 70 80 90 100
    IFMPKDGECH KLSNAALPLA MGHEMSGIVS KVGPKVTKVK VGDHVVVDAA
    110 120 130 140 150
    SSCADLHCWP HSKFYNSKPC DACQRGSENL CTHAGFVGLG VISGGFAEQV
    160 170 180 190 200
    VVSQHHIIPV PKEIPLDVAA LVEPLSVTWH AVKISGFKKG SSALVLGAGP
    210 220 230 240 250
    IGLCTILVLK GMGASKIVVS EIAERRIEMA KKLGVEVFNP SKHGHKSIEI
    260 270 280 290 300
    LRGLTKSHDG FDYSYDCSGI QVTFETSLKA LTFKGTATNI AVWGPKPVPF
    310 320 330 340 350
    QPMDVTLQEK VMTGSIGYVV EDFEEVVRAI HNGDIAMEDC KQLITGKQRI
    360 370 380
    EDGWEKGFQE LMDHKESNVK ILLTPNNHGE MK
    Length:382
    Mass (Da):41,582
    Last modified:July 27, 2011 - v2
    Checksum:i1776FA5968EC1A12
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti322D → A in AAC04974 (PubMed:7731988).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U12980 Genomic DNA Translation: AAC04974.1
    AY692922 Genomic DNA Translation: AAT92941.1
    BK006935 Genomic DNA Translation: DAA06929.2
    PIRiS51962
    RefSeqiNP_009341.2, NM_001178202.2

    Genome annotation databases

    EnsemblFungiiYAL060W; YAL060W; YAL060W
    GeneIDi851239
    KEGGisce:YAL060W

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U12980 Genomic DNA Translation: AAC04974.1
    AY692922 Genomic DNA Translation: AAT92941.1
    BK006935 Genomic DNA Translation: DAA06929.2
    PIRiS51962
    RefSeqiNP_009341.2, NM_001178202.2

    3D structure databases

    ProteinModelPortaliP39714
    SMRiP39714
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31770, 49 interactors
    DIPiDIP-5356N
    IntActiP39714, 1 interactor
    MINTiP39714
    STRINGi4932.YAL060W

    PTM databases

    CarbonylDBiP39714
    iPTMnetiP39714

    Proteomic databases

    MaxQBiP39714
    PaxDbiP39714
    PRIDEiP39714

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYAL060W; YAL060W; YAL060W
    GeneIDi851239
    KEGGisce:YAL060W

    Organism-specific databases

    SGDiS000000056 BDH1

    Phylogenomic databases

    GeneTreeiENSGT00910000146556
    HOGENOMiHOG000294670
    InParanoidiP39714
    KOiK00004
    OMAiGYPDEIF
    OrthoDBiEOG092C3CGE

    Enzyme and pathway databases

    BioCyciMetaCyc:YAL060W-MONOMER
    YEAST:YAL060W-MONOMER
    BRENDAi1.1.1.303 984
    1.1.1.4 984

    Miscellaneous databases

    PROiPR:P39714

    Family and domain databases

    InterProiView protein in InterPro
    IPR013149 ADH_C
    IPR013154 ADH_N
    IPR002328 ADH_Zn_CS
    IPR011032 GroES-like_sf
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF08240 ADH_N, 1 hit
    PF00107 ADH_zinc_N, 1 hit
    SUPFAMiSSF50129 SSF50129, 1 hit
    SSF51735 SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00059 ADH_ZINC, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBDH1_YEAST
    AccessioniPrimary (citable) accession number: P39714
    Secondary accession number(s): D6VPF9, E9P8Z3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: November 7, 2018
    This is version 160 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names
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