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Protein

Transcription factor MBP1

Gene

MBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi36 – 57H-T-H motifPROSITE-ProRule annotationAdd BLAST22

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific Source: SGD

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29472-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MBP1
Alternative name(s):
MBF subunit p120
Gene namesi
Name:MBP1
Ordered Locus Names:YDL056W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

SGDiS000002214 MBP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670621 – 833Transcription factor MBP1Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Modified residuei325PhosphothreonineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1
Modified residuei827PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39678
PaxDbiP39678
PRIDEiP39678

PTM databases

iPTMnetiP39678

Interactioni

Subunit structurei

Component of the transcription complex MCB-binding factor (MBF) composed of SWI6 and MBP1. Interacts with MSA1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SWI6P099594EBI-10485,EBI-18641

Protein-protein interaction databases

BioGridi32002, 156 interactors
ComplexPortaliCPX-950 MBP transcription complex
DIPiDIP-2391N
IntActiP39678, 29 interactors
MINTiP39678
STRINGi4932.YDL056W

Structurei

Secondary structure

1833
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP39678
SMRiP39678
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39678

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 111HTH APSES-typePROSITE-ProRule annotationAdd BLAST107
Repeati394 – 423ANK 1Add BLAST30
Repeati512 – 541ANK 2Add BLAST30

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00530000067565
HOGENOMiHOG000113493
InParanoidiP39678
KOiK06647
OMAiGFGKYQG
OrthoDBiEOG092C16YL

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
3.10.260.10, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR036887 HTH_APSES_sf
IPR018004 KilA_N/APSES_HTH
IPR029793 Mbp1/Res1/Res2
IPR003163 Tscrpt_reg_HTH_APSES-type
PANTHERiPTHR43828:SF1 PTHR43828:SF1, 1 hit
PfamiView protein in Pfam
PF04383 KilA-N, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM01252 KilA-N, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF54616 SSF54616, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS51299 HTH_APSES, 1 hit

Sequencei

Sequence statusi: Complete.

P39678-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNQIYSARY SGVDVYEFIH STGSIMKRKK DDWVNATHIL KAANFAKAKR
60 70 80 90 100
TRILEKEVLK ETHEKVQGGF GKYQGTWVPL NIAKQLAEKF SVYDQLKPLF
110 120 130 140 150
DFTQTDGSAS PPPAPKHHHA SKVDRKKAIR SASTSAIMET KRNNKKAEEN
160 170 180 190 200
QFQSSKILGN PTAAPRKRGR PVGSTRGSRR KLGVNLQRSQ SDMGFPRPAI
210 220 230 240 250
PNSSISTTQL PSIRSTMGPQ SPTLGILEEE RHDSRQQQPQ QNNSAQFKEI
260 270 280 290 300
DLEDGLSSDV EPSQQLQQVF NQNTGFVPQQ QSSLIQTQQT ESMATSVSSS
310 320 330 340 350
PSLPTSPGDF ADSNPFEERF PGGGTSPIIS MIPRYPVTSR PQTSDINDKV
360 370 380 390 400
NKYLSKLVDY FISNEMKSNK SLPQVLLHPP PHSAPYIDAP IDPELHTAFH
410 420 430 440 450
WACSMGNLPI AEALYEAGTS IRSTNSQGQT PLMRSSLFHN SYTRRTFPRI
460 470 480 490 500
FQLLHETVFD IDSQSQTVIH HIVKRKSTTP SAVYYLDVVL SKIKDFSPQY
510 520 530 540 550
RIELLLNTQD KNGDTALHIA SKNGDVVFFN TLVKMGALTT ISNKEGLTAN
560 570 580 590 600
EIMNQQYEQM MIQNGTNQHV NSSNTDLNIH VNTNNIETKN DVNSMVIMSP
610 620 630 640 650
VSPSDYITYP SQIATNISRN IPNVVNSMKQ MASIYNDLHE QHDNEIKSLQ
660 670 680 690 700
KTLKSISKTK IQVSLKTLEV LKESSKDENG EAQTNDDFEI LSRLQEQNTK
710 720 730 740 750
KLRKRLIRYK RLIKQKLEYR QTVLLNKLIE DETQATTNNT VEKDNNTLER
760 770 780 790 800
LELAQELTML QLQRKNKLSS LVKKFEDNAK IHKYRRIIRE GTEMNIEEVD
810 820 830
SSLDVILQTL IANNNKNKGA EQIITISNAN SHA
Length:833
Mass (Da):93,908
Last modified:February 1, 1995 - v1
Checksum:iBB7C35E29802BBD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74158 Genomic DNA Translation: CAA52271.1
Z74104 Genomic DNA Translation: CAA98618.1
U19608 Genomic DNA Translation: AAC49290.1
BK006938 Genomic DNA Translation: DAA11800.1
PIRiA47528
RefSeqiNP_010227.1, NM_001180115.1

Genome annotation databases

EnsemblFungiiYDL056W; YDL056W; YDL056W
GeneIDi851503
KEGGisce:YDL056W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74158 Genomic DNA Translation: CAA52271.1
Z74104 Genomic DNA Translation: CAA98618.1
U19608 Genomic DNA Translation: AAC49290.1
BK006938 Genomic DNA Translation: DAA11800.1
PIRiA47528
RefSeqiNP_010227.1, NM_001180115.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BM8X-ray1.71A4-102[»]
1L3GNMR-A2-124[»]
1MB1X-ray2.10A1-124[»]
ProteinModelPortaliP39678
SMRiP39678
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32002, 156 interactors
ComplexPortaliCPX-950 MBP transcription complex
DIPiDIP-2391N
IntActiP39678, 29 interactors
MINTiP39678
STRINGi4932.YDL056W

PTM databases

iPTMnetiP39678

Proteomic databases

MaxQBiP39678
PaxDbiP39678
PRIDEiP39678

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL056W; YDL056W; YDL056W
GeneIDi851503
KEGGisce:YDL056W

Organism-specific databases

SGDiS000002214 MBP1

Phylogenomic databases

GeneTreeiENSGT00530000067565
HOGENOMiHOG000113493
InParanoidiP39678
KOiK06647
OMAiGFGKYQG
OrthoDBiEOG092C16YL

Enzyme and pathway databases

BioCyciYEAST:G3O-29472-MONOMER

Miscellaneous databases

EvolutionaryTraceiP39678
PROiPR:P39678

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
3.10.260.10, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR036887 HTH_APSES_sf
IPR018004 KilA_N/APSES_HTH
IPR029793 Mbp1/Res1/Res2
IPR003163 Tscrpt_reg_HTH_APSES-type
PANTHERiPTHR43828:SF1 PTHR43828:SF1, 1 hit
PfamiView protein in Pfam
PF04383 KilA-N, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM01252 KilA-N, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF54616 SSF54616, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS51299 HTH_APSES, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMBP1_YEAST
AccessioniPrimary (citable) accession number: P39678
Secondary accession number(s): D6VRU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 7, 2018
This is version 187 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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