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Entry version 119 (12 Aug 2020)
Sequence version 1 (01 Feb 1995)
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Protein

FMN reductase (NADPH)

Gene

nfrA1

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Reduces FMNH2 to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes.1 Publication

Miscellaneous

Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism. NADPH is more effective as an electron donor than NADH.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.85 µM for NADPH (with nitrofurazone at pH 7 and at 23 degrees Celius)1 Publication
  2. KM=3.9 µM for NADPH (with FMN at pH 7 and at 23 degrees Celius)1 Publication
  3. KM=4.7 µM for FMN (with NADPH at pH 7 and at 23 degrees Celius)1 Publication
  4. KM=16.3 µM for nitrofurazone (with NADPH at pH 7 and at 23 degrees Celius)1 Publication
  1. Vmax=9 µmol/min/mg enzyme with FMN as substrate (with NADPH at pH 7 and at 23 degrees Celius)1 Publication
  2. Vmax=97 µmol/min/mg enzyme with nitrofurazone as substrate (with NADPH at pH 7 and at 23 degrees Celius)1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandFlavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU38110-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
FMN reductase (NADPH) (EC:1.5.1.381 Publication)
Alternative name(s):
NADPH-dependent FMN reductase
NADPH-dependent nitro/flavin reductase
NADPH-dependent nitroreductase
NADPH-dependent oxidoreductase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nfrA1
Synonyms:nfrA, ywcG
Ordered Locus Names:BSU38110
ORF Names:ipa-43d
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002055121 – 249FMN reductase (NADPH)Add BLAST249

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
P39605

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P39605

PRoteomics IDEntifications database

More...
PRIDEi
P39605

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

1 Publication

Protein-protein interaction databases

Molecular INTeraction database

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MINTi
P39605

STRING: functional protein association networks

More...
STRINGi
224308.BSU38110

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P39605

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
P39605

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the flavin oxidoreductase frp family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0778, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P39605

KEGG Orthology (KO)

More...
KOi
K19285

Identification of Orthologs from Complete Genome Data

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OMAi
GMCLGYP

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P39605

Family and domain databases

Conserved Domains Database

More...
CDDi
cd02146, NfsA_FRP, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.109.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016446, Flavin_OxRdtase_Frp
IPR029479, Nitroreductase
IPR000415, Nitroreductase-like

The PANTHER Classification System

More...
PANTHERi
PTHR43425, PTHR43425, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00881, Nitroreductase, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF005426, Frp, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55469, SSF55469, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P39605-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNNTIETILN HRSIRSFTDQ LLTAEEIDTL VKSAQAASTS SYVQAYSIIG
60 70 80 90 100
VSDPEKKREL SVLAGNQPYV EKNGHFFVFC ADLYRHQQLA EEKGEHISEL
110 120 130 140 150
LENTEMFMVS LIDAALAAQN MSIAAESMGL GICYIGGIRN ELDKVTEVLQ
160 170 180 190 200
TPDHVLPLFG LAVGHPANLS GKKPRLPKQA VYHENTYNVN TDDFRHTMNT
210 220 230 240
YDKTISDYYR ERTNGKREET WSDQILNFMK QKPRTYLNDY VKEKGFNKN
Length:249
Mass (Da):28,320
Last modified:February 1, 1995 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA36FDAECEC3692CF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X73124 Genomic DNA Translation: CAA51599.1
AL009126 Genomic DNA Translation: CAB15837.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S39698

NCBI Reference Sequences

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RefSeqi
NP_391690.1, NC_000964.3
WP_003244098.1, NZ_JNCM01000034.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB15837; CAB15837; BSU38110

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
937279

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU38110

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.4125

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA Translation: CAA51599.1
AL009126 Genomic DNA Translation: CAB15837.1
PIRiS39698
RefSeqiNP_391690.1, NC_000964.3
WP_003244098.1, NZ_JNCM01000034.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N2SX-ray1.95A/B/C/D1-249[»]
SMRiP39605
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

MINTiP39605
STRINGi224308.BSU38110

Proteomic databases

jPOSTiP39605
PaxDbiP39605
PRIDEiP39605

Genome annotation databases

EnsemblBacteriaiCAB15837; CAB15837; BSU38110
GeneIDi937279
KEGGibsu:BSU38110
PATRICifig|224308.179.peg.4125

Phylogenomic databases

eggNOGiCOG0778, Bacteria
InParanoidiP39605
KOiK19285
OMAiGMCLGYP
PhylomeDBiP39605

Enzyme and pathway databases

BioCyciBSUB:BSU38110-MONOMER

Miscellaneous databases

EvolutionaryTraceiP39605

Family and domain databases

CDDicd02146, NfsA_FRP, 1 hit
Gene3Di3.40.109.10, 1 hit
InterProiView protein in InterPro
IPR016446, Flavin_OxRdtase_Frp
IPR029479, Nitroreductase
IPR000415, Nitroreductase-like
PANTHERiPTHR43425, PTHR43425, 1 hit
PfamiView protein in Pfam
PF00881, Nitroreductase, 1 hit
PIRSFiPIRSF005426, Frp, 1 hit
SUPFAMiSSF55469, SSF55469, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNFRA1_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P39605
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: August 12, 2020
This is version 119 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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