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UniProtKB - P39552 (MST28_YEAST)
Protein
Multicopy suppressor of SEC21 protein 28
Gene
MST28
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in protein trafficking vesicle formation, probably by stabilizing of coatomer at the Golgi membrane and thus allowing the efficient formation of COPI coated vesicles.
1 PublicationMiscellaneous
Members of the DUP240 multigene family are specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240 proteins show no obvious alterations in mating, sporulation and cell growth.
GO - Biological processi
- protein transport Source: UniProtKB-KW
- vesicle-mediated transport Source: UniProtKB-KW
- vesicle organization Source: ComplexPortal
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Multicopy suppressor of SEC21 protein 28Alternative name(s): DUP240 protein MST28 |
Gene namesi | Name:MST28 Ordered Locus Names:YAR033W ORF Names:FUN59 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000079, MST28 |
VEuPathDBi | FungiDB:YAR033W |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 2 Publications
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- COPI-coated vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication
- COPII-coated vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Golgi apparatus
- Golgi apparatus Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- COPI-coated vesicle membrane Source: ComplexPortal
- ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
- integral component of membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 47 | CytoplasmicSequence analysisAdd BLAST | 47 | |
Transmembranei | 48 – 68 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 69 – 72 | ExtracellularSequence analysis | 4 | |
Transmembranei | 73 – 93 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 94 – 234 | CytoplasmicSequence analysisAdd BLAST | 141 |
Keywords - Cellular componenti
Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207526 | 1 – 234 | Multicopy suppressor of SEC21 protein 28Add BLAST | 234 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P39552 |
PaxDbi | P39552 |
PRIDEi | P39552 |
PTM databases
iPTMneti | P39552 |
Interactioni
Subunit structurei
Interacts with MST27. Binds to coatomer proteins of COPI and SEC23/SEC24 of COPII coated vesicles.
1 PublicationBinary interactionsi
P39552
With | #Exp. | IntAct |
---|---|---|
MST27 [P53176] | 4 | EBI-20785,EBI-6205 |
Protein-protein interaction databases
BioGRIDi | 31810, 58 interactors |
ComplexPortali | CPX-1303, MST27-MST28 vesicle formation complex |
DIPi | DIP-1853N |
IntActi | P39552, 6 interactors |
MINTi | P39552 |
STRINGi | 4932.YAR033W |
Miscellaneous databases
RNActi | P39552, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 231 – 234 | COPI binding | 4 |
Sequence similaritiesi
Belongs to the DUP/COS family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SSNW, Eukaryota |
GeneTreei | ENSGT00940000176285 |
HOGENOMi | CLU_081384_0_1_1 |
InParanoidi | P39552 |
Family and domain databases
InterProi | View protein in InterPro IPR001142, DUP/COS |
Pfami | View protein in Pfam PF00674, DUP, 1 hit |
i Sequence
Sequence statusi: Complete.
P39552-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQTPPESTDV KLDTLNEPSA HLIEKNVALP KDIFRSYLSY WIYEIARYTP
60 70 80 90 100
VMILSLVIGV LVLLIIFFND NEACVFNSAI FAFTSLVGLL IILSDGNPKL
110 120 130 140 150
VSRRNFRTEL LVDVITRKPA VEGKEWRIIT YNMNQYLFNH GQWHTPYYFY
160 170 180 190 200
SDEDCYRYFL RLVEGVTPKK QTATSIGNSP VTAKPEDAIE SASPSSRLNY
210 220 230
QNFLLKAAEI ERQAQENYWR RRHPNIDALL KKTE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28920 Genomic DNA Translation: AAC09494.1 BK006935 Genomic DNA Translation: DAA07003.1 |
PIRi | S53484 |
RefSeqi | NP_009419.1, NM_001178225.1 |
Genome annotation databases
EnsemblFungii | YAR033W_mRNA; YAR033W; YAR033W |
GeneIDi | 851284 |
KEGGi | sce:YAR033W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L28920 Genomic DNA Translation: AAC09494.1 BK006935 Genomic DNA Translation: DAA07003.1 |
PIRi | S53484 |
RefSeqi | NP_009419.1, NM_001178225.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 31810, 58 interactors |
ComplexPortali | CPX-1303, MST27-MST28 vesicle formation complex |
DIPi | DIP-1853N |
IntActi | P39552, 6 interactors |
MINTi | P39552 |
STRINGi | 4932.YAR033W |
PTM databases
iPTMneti | P39552 |
Proteomic databases
MaxQBi | P39552 |
PaxDbi | P39552 |
PRIDEi | P39552 |
Genome annotation databases
EnsemblFungii | YAR033W_mRNA; YAR033W; YAR033W |
GeneIDi | 851284 |
KEGGi | sce:YAR033W |
Organism-specific databases
SGDi | S000000079, MST28 |
VEuPathDBi | FungiDB:YAR033W |
Phylogenomic databases
eggNOGi | ENOG502SSNW, Eukaryota |
GeneTreei | ENSGT00940000176285 |
HOGENOMi | CLU_081384_0_1_1 |
InParanoidi | P39552 |
Miscellaneous databases
PROi | PR:P39552 |
RNActi | P39552, protein |
Family and domain databases
InterProi | View protein in InterPro IPR001142, DUP/COS |
Pfami | View protein in Pfam PF00674, DUP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MST28_YEAST | |
Accessioni | P39552Primary (citable) accession number: P39552 Secondary accession number(s): D6VPN3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome I
Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names - SIMILARITY comments
Index of protein domains and families