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Protein

Homocitrate dehydratase, mitochondrial

Gene

ACO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible dehydration of (R)-homocitrate to cis-homoaconitate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.1 Publication

Miscellaneous

The fermenting yeast S.cerevisiae has 2 aconitases, ACO1 essential for the citric acid cycle, and ACO2 specifically and exclusively contributing to lysine biosynthesis. In contrast, in respiring filamentous fungi the ACO2 homologs (acoB) seem enzymatically inactive and the ACO1 homolog (acoA) is solely responsible for these functions.
Present with 4670 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(R)-2-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 2 of the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Homocitrate synthase, cytosolic isozyme (LYS20), Homocitrate synthase, mitochondrial (LYS21)
  2. Homocitrate dehydratase, mitochondrial (ACO2)
  3. Homoaconitase, mitochondrial (LYS4)
  4. Homoisocitrate dehydrogenase, mitochondrial (LYS12)
  5. Aromatic/aminoadipate aminotransferase 1 (ARO8)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alpha-aminoadipate from 2-oxoglutarate, the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Metal bindingi385Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi448Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi451Iron-sulfur (4Fe-4S)By similarity1
Binding sitei476SubstrateBy similarity1
Binding sitei481SubstrateBy similarity1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • aconitate hydratase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Lysine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YJL200C-MONOMER
ReactomeiR-SCE-71403 Citric acid cycle (TCA cycle)
R-SCE-917937 Iron uptake and transport
UniPathwayiUPA00033; UER00029

Protein family/group databases

MoonProtiP39533

Names & Taxonomyi

Protein namesi
Recommended name:
Homocitrate dehydratase, mitochondrial (EC:4.2.1.-)
Alternative name(s):
Aconitase 2
Gene namesi
Name:ACO2
Ordered Locus Names:YJL200C
ORF Names:J0327
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL200C
SGDiS000003736 ACO2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Has very little effect on growth on nonfermentable carbon sources.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi610K → R: Reduces catalytic activity towards homoaconitate by 45% and increases the activity towards aconitate by a factor 116. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 14MitochondrionSequence analysisAdd BLAST14
ChainiPRO_000007664915 – 789Homocitrate dehydratase, mitochondrialAdd BLAST775

Proteomic databases

MaxQBiP39533
PaxDbiP39533
PRIDEiP39533

PTM databases

iPTMnetiP39533

Expressioni

Inductioni

Constitutively expressed with a small induction when both glutamate and lysine are missing.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi33557, 72 interactors
DIPiDIP-1376N
IntActiP39533, 11 interactors
MINTiP39533
STRINGi4932.YJL200C

Structurei

3D structure databases

ProteinModelPortaliP39533
SMRiP39533
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 191Substrate bindingBy similarity3
Regioni672 – 673Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00920000149090
HOGENOMiHOG000224293
InParanoidiP39533
KOiK17450
OMAiQPRFYGC
OrthoDBiEOG092C0DQS

Family and domain databases

Gene3Di3.20.19.10, 1 hit
3.30.499.10, 2 hits
3.40.1060.10, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR015932 Aconitase_dom2
IPR006248 Aconitase_mito-like
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01340 aconitase_mito, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSANRFYI KRHLATHANM FPSVSKNFQT KVPPYAKLLT NLDKIKQITN
60 70 80 90 100
NAPLTLAEKI LYSHLCDPEE SITSSDLSTI RGNKYLKLNP DRVAMQDASA
110 120 130 140 150
QMALLQFMTT GLNQTSVPAS IHCDHLIVGK DGETKDLPSS IATNQEVFDF
160 170 180 190 200
LESCAKRYGI QFWGPGSGII HQIVLENFSA PGLMMLGTDS HTPNAGGLGA
210 220 230 240 250
IAIGVGGADA VDALTGTPWE LKAPKILGVK LTGKLNGWST PKDVITKLAG
260 270 280 290 300
LLTVRGGTGY IVEYFGEGVS TLSCTGMATI CNMGAEIGAT TSTFPYQEAH
310 320 330 340 350
KRYLQATNRA EVAEAADVAL NKFNFLRADK DAQYDKVIEI DLSAIEPHVN
360 370 380 390 400
GPFTPDLSTP ISQYAEKSLK ENWPQKVSAG LIGSCTNSSY QDMSRVVDLV
410 420 430 440 450
KQASKAGLKP RIPFFVTPGS EQIRATLERD GIIDIFQENG AKVLANACGP
460 470 480 490 500
CIGQWNREDV SKTSKETNTI FTSFNRNFRA RNDGNRNTMN FLTSPEIVTA
510 520 530 540 550
MSYSGDAQFN PLTDSIKLPN GKDFKFQPPK GDELPKRGFE HGRDKFYPEM
560 570 580 590 600
DPKPDSNVEI KVDPNSDRLQ LLEPFKPWNG KELKTNVLLK VEGKCTTDHI
610 620 630 640 650
SAAGVWLKYK GHLENISYNT LIGAQNKETG EVNKAYDLDG TEYDIPGLMM
660 670 680 690 700
KWKSDGRPWT VIAEHNYGEG SAREHAALSP RFLGGEILLV KSFARIHETN
710 720 730 740 750
LKKQGVLPLT FANESDYDKI SSGDVLETLN LVDMIAKDGN NGGEIDVKIT
760 770 780
KPNGESFTIK AKHTMSKDQI DFFKAGSAIN YIGNIRRNE
Length:789
Mass (Da):86,583
Last modified:February 1, 1995 - v1
Checksum:iFABA4FE482D3F993
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77688 Genomic DNA Translation: CAA54757.1
Z49475 Genomic DNA Translation: CAA89495.1
BK006943 Genomic DNA Translation: DAA08609.1
PIRiS46631
RefSeqiNP_012335.1, NM_001181633.1

Genome annotation databases

EnsemblFungiiYJL200C; YJL200C; YJL200C
GeneIDi853230
KEGGisce:YJL200C

Similar proteinsi

Entry informationi

Entry nameiACON2_YEAST
AccessioniPrimary (citable) accession number: P39533
Secondary accession number(s): D6VVZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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